SubmitCancel

Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

D2WL32

- GLGB3_ARATH

UniProt

D2WL32 - GLGB3_ARATH

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

1,4-alpha-glucan-branching enzyme 3, chloroplastic/amyloplastic

Gene
SBE3, BE1, EMB2729, At3g20440, MQC12.20
Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Catalyzes the formation of the alpha-1,6-glucosidic linkages in starch by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position. Essential during embryogenesis.2 Publications

Catalytic activityi

Transfers a segment of a (1->4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei612 – 6121Proton donor By similarity

GO - Molecular functioni

  1. 1,4-alpha-glucan branching enzyme activity Source: UniProtKB-EC
  2. cation binding Source: InterPro

GO - Biological processi

  1. carbohydrate metabolic process Source: TAIR
  2. post-embryonic development Source: TAIR
  3. starch biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Enzyme and pathway databases

BioCyciARA:AT3G20440-MONOMER.
ARA:GQT-615-MONOMER.
UniPathwayiUPA00152.

Protein family/group databases

CAZyiCBM48. Carbohydrate-Binding Module Family 48.
GH13. Glycoside Hydrolase Family 13.

Names & Taxonomyi

Protein namesi
Recommended name:
1,4-alpha-glucan-branching enzyme 3, chloroplastic/amyloplastic (EC:2.4.1.18)
Short name:
AtSBE III
Alternative name(s):
Branching enzyme 1
Short name:
AtBE1
Protein EMBRYO DEFECTIVE 2729
Starch-branching enzyme 3
Gene namesi
Name:SBE3
Synonyms:BE1, EMB2729
Ordered Locus Names:At3g20440
ORF Names:MQC12.20
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 3

Organism-specific databases

TAIRiAT3G20440.

Subcellular locationi

Plastidchloroplast stroma. Plastidamyloplast By similarity 3 Publications

GO - Cellular componenti

  1. amyloplast Source: UniProtKB-SubCell
  2. chloroplast Source: TAIR
  3. chloroplast stroma Source: UniProtKB-SubCell
  4. plastid Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Amyloplast, Chloroplast, Plastid

Pathology & Biotechi

Disruption phenotypei

Embryolethal. Embryo developmental arrests at the heart stage associated with reduced cell divisions and abnormal cell differentiation, thereby leading to defects in setting up the shoot apical meristem, embryonic vascular tissues and cotyledons. Modified starch composition. This phenotype is enhanced when associated with SBE2.1 and SBE2.2 disruptions.2 Publications

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi366 – 3661E → K in be1-3; reduced level of BE1 transcription and various developmental defects during post-germination growth such as shorter primary roots, delayed greening of cotyledons and leaf development, yellowish and small leaves leading to smaller plants with altered petals and sepals in flowers. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 4949Chloroplast Reviewed predictionAdd
BLAST
Chaini50 – 8998501,4-alpha-glucan-branching enzyme 3, chloroplastic/amyloplasticPRO_0000415337Add
BLAST

Proteomic databases

PRIDEiD2WL32.

Expressioni

Tissue specificityi

Mostly expressed in flowers and inflorescence, and, to a lower extent, in seedlings, roots, stems, leaves, siliques and seeds.2 Publications

Interactioni

Subunit structurei

Monomer By similarity.

Protein-protein interaction databases

STRINGi3702.AT3G20440.2-P.

Structurei

3D structure databases

ProteinModelPortaliD2WL32.
SMRiD2WL32. Positions 336-892.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiCOG0296.
HOGENOMiHOG000175159.
InParanoidiQ8GWK4.
OMAiRSISKRI.

Family and domain databases

Gene3Di2.60.40.10. 1 hit.
2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
InterProiIPR006048. A-amylase_b_C.
IPR015902. Glyco_hydro_13.
IPR013780. Glyco_hydro_13_b.
IPR006047. Glyco_hydro_13_cat_dom.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR013783. Ig-like_fold.
[Graphical view]
PANTHERiPTHR10357. PTHR10357. 1 hit.
PfamiPF00128. Alpha-amylase. 1 hit.
PF02806. Alpha-amylase_C. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 1 hit.

Sequences (3)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 3 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: D2WL32-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MVSLSNQTRF SFHPNNLVVS EKRRLGISGV NFPRKIKLKI TCFAAERPRQ    50
EKQKKKSQSQ STSDAEAGVD PVGFLTRLGI ADRIFAQFLR ERHKALKDLK 100
DEIFKRHFDF RDFASGFELL GMHRHMEHRV DFMDWGPGSR YGAIIGDFNG 150
WSPTENAARE GLFGHDDYGY WFIILEDKLR EGEEPDELYF QQYNYVDDYD 200
KGDSGVSAEE IFQKANDEYW EPGEDRFIKN RFEVPAKLYE QMFGPNSPQT 250
LEELGDIPDA ETRYKQWKEE HKDDPPSNLP PCDIIDKGQG KPYDIFNVVT 300
SPEWTKKFYE KEPPIPYWLE TRKGRKAWLQ KYIPAVPHGS KYRLYFNTPD 350
GPLERVPAWA TYVQPEDEGK QAYAIHWEPS PEAAYKWKYS KPKVPESLRI 400
YECHVGISGS EPKVSTFEEF TKKVLPHVKR AGYNAIQLIG VPEHKDYFTV 450
GYRVTNFFAA SSRYGTPDDF KRLVDEAHGL GLLVFLDIVH SYAAADQMVG 500
LSLFDGSNDC YFHYGKRGHH KHWGTRMFKY GDLDVLHFLI SNLNWWITEY 550
QVDGYQFHSL ASMIYTHNGF ASFNNDLDDY CNQYVDRDAL MYLILANEIL 600
HVQHPNIITI AEDATYYPGL CEPVSQGGLG FDYYVNLSAS EMWVSLLDNV 650
PDNEWSMSKI VSTLVANKEY ADKMLSYAEN HNQSISGGRS FAEILFGGVD 700
NGSPGGKELL DRGISLHKMI RLITFTSGGR AYLNFMGNEF GHPERVEFPT 750
QSNNFSFSLA NRRWDLLESG VHHHLFSFDK ELMDLDKSKG ILSRGLPSIH 800
HVNDANMVIS FSRGPFLFIF NFHPSNSYEK YDVGVEEAGE YTMILNSDEV 850
KYGGQGIVTE DHYLQRSISK RIDGQRNCLE VFLPSRTAQV YKLTRILRI 899
Length:899
Mass (Da):103,525
Last modified:March 2, 2010 - v1
Checksum:i6C047303FD5D924E
GO
Isoform 2 (identifier: D2WL32-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     424-453: Missing.

Note: No experimental confirmation available.

Show »
Length:869
Mass (Da):100,101
Checksum:iAC9CB03532EBDD4C
GO
Isoform 3 (identifier: D2WL32-3) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     365-365: P → PGMTAF
     780-780: K → KVS
     881-889: Missing.

Note: No experimental confirmation available.

Show »
Length:897
Mass (Da):103,219
Checksum:i0BD1979DB44C356F
GO

Sequence cautioni

The sequence BAB02827.1 differs from that shown. Reason: Erroneous gene model prediction.

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei365 – 3651P → PGMTAF in isoform 3. VSP_042216
Alternative sequencei424 – 45330Missing in isoform 2. VSP_042217Add
BLAST
Alternative sequencei780 – 7801K → KVS in isoform 3. VSP_042218
Alternative sequencei881 – 8899Missing in isoform 3. VSP_042219

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
GQ374452 mRNA. Translation: ADB29066.1.
AB024036 Genomic DNA. Translation: BAB02827.1. Sequence problems.
CP002686 Genomic DNA. Translation: AEE76378.1.
CP002686 Genomic DNA. Translation: AEE76379.1.
CP002686 Genomic DNA. Translation: AEE76380.1.
AK118785 mRNA. Translation: BAC43378.1.
RefSeqiNP_001154629.1. NM_001161157.1. [D2WL32-1]
NP_001189940.1. NM_001203011.1. [D2WL32-3]
NP_188679.2. NM_112935.2. [D2WL32-2]
UniGeneiAt.38239.

Genome annotation databases

EnsemblPlantsiAT3G20440.2; AT3G20440.2; AT3G20440. [D2WL32-1]
GeneIDi821589.
KEGGiath:AT3G20440.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
GQ374452 mRNA. Translation: ADB29066.1 .
AB024036 Genomic DNA. Translation: BAB02827.1 . Sequence problems.
CP002686 Genomic DNA. Translation: AEE76378.1 .
CP002686 Genomic DNA. Translation: AEE76379.1 .
CP002686 Genomic DNA. Translation: AEE76380.1 .
AK118785 mRNA. Translation: BAC43378.1 .
RefSeqi NP_001154629.1. NM_001161157.1. [D2WL32-1 ]
NP_001189940.1. NM_001203011.1. [D2WL32-3 ]
NP_188679.2. NM_112935.2. [D2WL32-2 ]
UniGenei At.38239.

3D structure databases

ProteinModelPortali D2WL32.
SMRi D2WL32. Positions 336-892.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 3702.AT3G20440.2-P.

Protein family/group databases

CAZyi CBM48. Carbohydrate-Binding Module Family 48.
GH13. Glycoside Hydrolase Family 13.

Proteomic databases

PRIDEi D2WL32.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT3G20440.2 ; AT3G20440.2 ; AT3G20440 . [D2WL32-1 ]
GeneIDi 821589.
KEGGi ath:AT3G20440.

Organism-specific databases

TAIRi AT3G20440.

Phylogenomic databases

eggNOGi COG0296.
HOGENOMi HOG000175159.
InParanoidi Q8GWK4.
OMAi RSISKRI.

Enzyme and pathway databases

UniPathwayi UPA00152 .
BioCyci ARA:AT3G20440-MONOMER.
ARA:GQT-615-MONOMER.

Family and domain databases

Gene3Di 2.60.40.10. 1 hit.
2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
InterProi IPR006048. A-amylase_b_C.
IPR015902. Glyco_hydro_13.
IPR013780. Glyco_hydro_13_b.
IPR006047. Glyco_hydro_13_cat_dom.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR013783. Ig-like_fold.
[Graphical view ]
PANTHERi PTHR10357. PTHR10357. 1 hit.
Pfami PF00128. Alpha-amylase. 1 hit.
PF02806. Alpha-amylase_C. 1 hit.
[Graphical view ]
SUPFAMi SSF51445. SSF51445. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The Arabidopsis BE1 gene, encoding a putative glycoside hydrolase localized in plastids, plays crucial roles during embryogenesis and carbohydrate metabolism."
    Wang X., Xue L., Sun J., Zuo J.
    J. Integr. Plant Biol. 52:273-288(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, MUTAGENESIS OF GLU-366, DISRUPTION PHENOTYPE.
    Strain: cv. Columbia.
  2. "Structural analysis of Arabidopsis thaliana chromosome 3. I. Sequence features of the regions of 4,504,864 bp covered by sixty P1 and TAC clones."
    Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Tabata S.
    DNA Res. 7:131-135(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  3. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Strain: cv. Columbia.
  5. "Mutants of Arabidopsis lacking starch branching enzyme II substitute plastidial starch synthesis by cytoplasmic maltose accumulation."
    Dumez S., Wattebled F., Dauvillee D., Delvalle D., Planchot V., Ball S.G., D'Hulst C.
    Plant Cell 18:2694-2709(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DISRUPTION PHENOTYPE.
    Strain: cv. Columbia and cv. Wassilewskija.
  6. "Three orthologs in rice, Arabidopsis, and Populus encoding starch branching enzymes (SBEs) are different from other SBE gene families in plants."
    Han Y., Sun F.-J., Rosales-Mendoza S., Korban S.S.
    Gene 401:123-130(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY, GENE FAMILY, NOMENCLATURE.
    Strain: cv. Columbia.
  7. "Sorting signals, N-terminal modifications and abundance of the chloroplast proteome."
    Zybailov B., Rutschow H., Friso G., Rudella A., Emanuelsson O., Sun Q., van Wijk K.J.
    PLoS ONE 3:E1994-E1994(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    Strain: cv. Columbia.
  8. "Megadalton complexes in the chloroplast stroma of Arabidopsis thaliana characterized by size exclusion chromatography, mass spectrometry, and hierarchical clustering."
    Olinares P.D., Ponnala L., van Wijk K.J.
    Mol. Cell. Proteomics 9:1594-1615(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    Strain: cv. Columbia.

Entry informationi

Entry nameiGLGB3_ARATH
AccessioniPrimary (citable) accession number: D2WL32
Secondary accession number(s): F4JEP1, Q8GWK4, Q9LTP8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 25, 2012
Last sequence update: March 2, 2010
Last modified: June 11, 2014
This is version 43 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  3. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  4. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi