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Protein

1,4-alpha-glucan-branching enzyme 3, chloroplastic/amyloplastic

Gene

SBE3

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the formation of the alpha-1,6-glucosidic linkages in starch by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position. Essential during embryogenesis.2 Publications

Catalytic activityi

Transfers a segment of a (1->4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei612 – 6121Proton donorBy similarity

GO - Molecular functioni

  1. 1,4-alpha-glucan branching enzyme activity Source: UniProtKB-EC
  2. cation binding Source: InterPro

GO - Biological processi

  1. carbohydrate metabolic process Source: TAIR
  2. glycogen biosynthetic process Source: InterPro
  3. post-embryonic development Source: TAIR
  4. starch biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Enzyme and pathway databases

BioCyciARA:AT3G20440-MONOMER.
ARA:GQT-615-MONOMER.
UniPathwayiUPA00152.

Protein family/group databases

CAZyiCBM48. Carbohydrate-Binding Module Family 48.
GH13. Glycoside Hydrolase Family 13.

Names & Taxonomyi

Protein namesi
Recommended name:
1,4-alpha-glucan-branching enzyme 3, chloroplastic/amyloplastic (EC:2.4.1.18)
Short name:
AtSBE III
Alternative name(s):
Branching enzyme 1
Short name:
AtBE1
Protein EMBRYO DEFECTIVE 2729
Starch-branching enzyme 3
Gene namesi
Name:SBE3
Synonyms:BE1, EMB2729
Ordered Locus Names:At3g20440
ORF Names:MQC12.20
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 3

Organism-specific databases

TAIRiAT3G20440.

Subcellular locationi

Plastidchloroplast stroma 3 Publications. Plastidamyloplast By similarity

GO - Cellular componenti

  1. amyloplast Source: UniProtKB-SubCell
  2. chloroplast Source: TAIR
  3. chloroplast stroma Source: UniProtKB-SubCell
  4. plastid Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Amyloplast, Chloroplast, Plastid

Pathology & Biotechi

Disruption phenotypei

Embryolethal. Embryo developmental arrests at the heart stage associated with reduced cell divisions and abnormal cell differentiation, thereby leading to defects in setting up the shoot apical meristem, embryonic vascular tissues and cotyledons. Modified starch composition. This phenotype is enhanced when associated with SBE2.1 and SBE2.2 disruptions.2 Publications

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi366 – 3661E → K in be1-3; reduced level of BE1 transcription and various developmental defects during post-germination growth such as shorter primary roots, delayed greening of cotyledons and leaf development, yellowish and small leaves leading to smaller plants with altered petals and sepals in flowers. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 4949ChloroplastSequence AnalysisAdd
BLAST
Chaini50 – 8998501,4-alpha-glucan-branching enzyme 3, chloroplastic/amyloplasticPRO_0000415337Add
BLAST

Proteomic databases

PRIDEiD2WL32.

Expressioni

Tissue specificityi

Mostly expressed in flowers and inflorescence, and, to a lower extent, in seedlings, roots, stems, leaves, siliques and seeds.2 Publications

Interactioni

Subunit structurei

Monomer.By similarity

Protein-protein interaction databases

STRINGi3702.AT3G20440.2-P.

Structurei

3D structure databases

ProteinModelPortaliD2WL32.
SMRiD2WL32. Positions 336-892.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiCOG0296.
HOGENOMiHOG000175159.
InParanoidiD2WL32.
OMAiNWWVTEY.

Family and domain databases

Gene3Di2.60.40.10. 1 hit.
2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
InterProiIPR006048. A-amylase_b_C.
IPR015902. Glyco_hydro_13.
IPR013780. Glyco_hydro_13_b.
IPR006047. Glyco_hydro_13_cat_dom.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR013783. Ig-like_fold.
[Graphical view]
PANTHERiPTHR10357. PTHR10357. 1 hit.
PfamiPF00128. Alpha-amylase. 1 hit.
PF02806. Alpha-amylase_C. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 1 hit.

Sequences (3)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: D2WL32-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MVSLSNQTRF SFHPNNLVVS EKRRLGISGV NFPRKIKLKI TCFAAERPRQ
60 70 80 90 100
EKQKKKSQSQ STSDAEAGVD PVGFLTRLGI ADRIFAQFLR ERHKALKDLK
110 120 130 140 150
DEIFKRHFDF RDFASGFELL GMHRHMEHRV DFMDWGPGSR YGAIIGDFNG
160 170 180 190 200
WSPTENAARE GLFGHDDYGY WFIILEDKLR EGEEPDELYF QQYNYVDDYD
210 220 230 240 250
KGDSGVSAEE IFQKANDEYW EPGEDRFIKN RFEVPAKLYE QMFGPNSPQT
260 270 280 290 300
LEELGDIPDA ETRYKQWKEE HKDDPPSNLP PCDIIDKGQG KPYDIFNVVT
310 320 330 340 350
SPEWTKKFYE KEPPIPYWLE TRKGRKAWLQ KYIPAVPHGS KYRLYFNTPD
360 370 380 390 400
GPLERVPAWA TYVQPEDEGK QAYAIHWEPS PEAAYKWKYS KPKVPESLRI
410 420 430 440 450
YECHVGISGS EPKVSTFEEF TKKVLPHVKR AGYNAIQLIG VPEHKDYFTV
460 470 480 490 500
GYRVTNFFAA SSRYGTPDDF KRLVDEAHGL GLLVFLDIVH SYAAADQMVG
510 520 530 540 550
LSLFDGSNDC YFHYGKRGHH KHWGTRMFKY GDLDVLHFLI SNLNWWITEY
560 570 580 590 600
QVDGYQFHSL ASMIYTHNGF ASFNNDLDDY CNQYVDRDAL MYLILANEIL
610 620 630 640 650
HVQHPNIITI AEDATYYPGL CEPVSQGGLG FDYYVNLSAS EMWVSLLDNV
660 670 680 690 700
PDNEWSMSKI VSTLVANKEY ADKMLSYAEN HNQSISGGRS FAEILFGGVD
710 720 730 740 750
NGSPGGKELL DRGISLHKMI RLITFTSGGR AYLNFMGNEF GHPERVEFPT
760 770 780 790 800
QSNNFSFSLA NRRWDLLESG VHHHLFSFDK ELMDLDKSKG ILSRGLPSIH
810 820 830 840 850
HVNDANMVIS FSRGPFLFIF NFHPSNSYEK YDVGVEEAGE YTMILNSDEV
860 870 880 890
KYGGQGIVTE DHYLQRSISK RIDGQRNCLE VFLPSRTAQV YKLTRILRI
Length:899
Mass (Da):103,525
Last modified:March 2, 2010 - v1
Checksum:i6C047303FD5D924E
GO
Isoform 2 (identifier: D2WL32-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     424-453: Missing.

Note: No experimental confirmation available.

Show »
Length:869
Mass (Da):100,101
Checksum:iAC9CB03532EBDD4C
GO
Isoform 3 (identifier: D2WL32-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     365-365: P → PGMTAF
     780-780: K → KVS
     881-889: Missing.

Note: No experimental confirmation available.

Show »
Length:897
Mass (Da):103,219
Checksum:i0BD1979DB44C356F
GO

Sequence cautioni

The sequence BAB02827.1 differs from that shown. Reason: Erroneous gene model prediction. Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei365 – 3651P → PGMTAF in isoform 3. CuratedVSP_042216
Alternative sequencei424 – 45330Missing in isoform 2. 1 PublicationVSP_042217Add
BLAST
Alternative sequencei780 – 7801K → KVS in isoform 3. CuratedVSP_042218
Alternative sequencei881 – 8899Missing in isoform 3. CuratedVSP_042219

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
GQ374452 mRNA. Translation: ADB29066.1.
AB024036 Genomic DNA. Translation: BAB02827.1. Sequence problems.
CP002686 Genomic DNA. Translation: AEE76378.1.
CP002686 Genomic DNA. Translation: AEE76379.1.
CP002686 Genomic DNA. Translation: AEE76380.1.
AK118785 mRNA. Translation: BAC43378.1.
RefSeqiNP_001154629.1. NM_001161157.1. [D2WL32-1]
NP_001189940.1. NM_001203011.1. [D2WL32-3]
NP_188679.2. NM_112935.2. [D2WL32-2]
UniGeneiAt.38239.

Genome annotation databases

EnsemblPlantsiAT3G20440.2; AT3G20440.2; AT3G20440. [D2WL32-1]
GeneIDi821589.
KEGGiath:AT3G20440.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
GQ374452 mRNA. Translation: ADB29066.1.
AB024036 Genomic DNA. Translation: BAB02827.1. Sequence problems.
CP002686 Genomic DNA. Translation: AEE76378.1.
CP002686 Genomic DNA. Translation: AEE76379.1.
CP002686 Genomic DNA. Translation: AEE76380.1.
AK118785 mRNA. Translation: BAC43378.1.
RefSeqiNP_001154629.1. NM_001161157.1. [D2WL32-1]
NP_001189940.1. NM_001203011.1. [D2WL32-3]
NP_188679.2. NM_112935.2. [D2WL32-2]
UniGeneiAt.38239.

3D structure databases

ProteinModelPortaliD2WL32.
SMRiD2WL32. Positions 336-892.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi3702.AT3G20440.2-P.

Protein family/group databases

CAZyiCBM48. Carbohydrate-Binding Module Family 48.
GH13. Glycoside Hydrolase Family 13.

Proteomic databases

PRIDEiD2WL32.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT3G20440.2; AT3G20440.2; AT3G20440. [D2WL32-1]
GeneIDi821589.
KEGGiath:AT3G20440.

Organism-specific databases

TAIRiAT3G20440.

Phylogenomic databases

eggNOGiCOG0296.
HOGENOMiHOG000175159.
InParanoidiD2WL32.
OMAiNWWVTEY.

Enzyme and pathway databases

UniPathwayiUPA00152.
BioCyciARA:AT3G20440-MONOMER.
ARA:GQT-615-MONOMER.

Family and domain databases

Gene3Di2.60.40.10. 1 hit.
2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
InterProiIPR006048. A-amylase_b_C.
IPR015902. Glyco_hydro_13.
IPR013780. Glyco_hydro_13_b.
IPR006047. Glyco_hydro_13_cat_dom.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR013783. Ig-like_fold.
[Graphical view]
PANTHERiPTHR10357. PTHR10357. 1 hit.
PfamiPF00128. Alpha-amylase. 1 hit.
PF02806. Alpha-amylase_C. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The Arabidopsis BE1 gene, encoding a putative glycoside hydrolase localized in plastids, plays crucial roles during embryogenesis and carbohydrate metabolism."
    Wang X., Xue L., Sun J., Zuo J.
    J. Integr. Plant Biol. 52:273-288(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, MUTAGENESIS OF GLU-366, DISRUPTION PHENOTYPE.
    Strain: cv. Columbia.
  2. "Structural analysis of Arabidopsis thaliana chromosome 3. I. Sequence features of the regions of 4,504,864 bp covered by sixty P1 and TAC clones."
    Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Tabata S.
    DNA Res. 7:131-135(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  3. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Strain: cv. Columbia.
  5. "Mutants of Arabidopsis lacking starch branching enzyme II substitute plastidial starch synthesis by cytoplasmic maltose accumulation."
    Dumez S., Wattebled F., Dauvillee D., Delvalle D., Planchot V., Ball S.G., D'Hulst C.
    Plant Cell 18:2694-2709(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DISRUPTION PHENOTYPE.
    Strain: cv. Columbia and cv. Wassilewskija.
  6. "Three orthologs in rice, Arabidopsis, and Populus encoding starch branching enzymes (SBEs) are different from other SBE gene families in plants."
    Han Y., Sun F.-J., Rosales-Mendoza S., Korban S.S.
    Gene 401:123-130(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY, GENE FAMILY, NOMENCLATURE.
    Strain: cv. Columbia.
  7. "Sorting signals, N-terminal modifications and abundance of the chloroplast proteome."
    Zybailov B., Rutschow H., Friso G., Rudella A., Emanuelsson O., Sun Q., van Wijk K.J.
    PLoS ONE 3:E1994-E1994(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    Strain: cv. Columbia.
  8. "Megadalton complexes in the chloroplast stroma of Arabidopsis thaliana characterized by size exclusion chromatography, mass spectrometry, and hierarchical clustering."
    Olinares P.D., Ponnala L., van Wijk K.J.
    Mol. Cell. Proteomics 9:1594-1615(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    Strain: cv. Columbia.

Entry informationi

Entry nameiGLGB3_ARATH
AccessioniPrimary (citable) accession number: D2WL32
Secondary accession number(s): F4JEP1, Q8GWK4, Q9LTP8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 25, 2012
Last sequence update: March 2, 2010
Last modified: March 4, 2015
This is version 47 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  3. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  4. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.