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D2WKD8

- AT1A2_PIG

UniProt

D2WKD8 - AT1A2_PIG

Protein

Sodium/potassium-transporting ATPase subunit alpha-2

Gene

ATP1A2

Organism
Sus scrofa (Pig)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 37 (01 Oct 2014)
      Sequence version 1 (02 Mar 2010)
      Previous versions | rss
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    Functioni

    This is the catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. This action creates the electrochemical gradient of sodium and potassium, providing the energy for active transport of various nutrients By similarity.By similarity

    Catalytic activityi

    ATP + H2O + Na+(In) + K+(Out) = ADP + phosphate + Na+(Out) + K+(In).

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei374 – 37414-aspartylphosphate intermediateBy similarity
    Metal bindingi714 – 7141MagnesiumBy similarity
    Metal bindingi718 – 7181MagnesiumBy similarity

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. metal ion binding Source: UniProtKB-KW
    3. sodium:potassium-exchanging ATPase activity Source: UniProtKB-EC

    GO - Biological processi

    1. adult locomotory behavior Source: Ensembl
    2. ATP biosynthetic process Source: InterPro
    3. locomotion Source: Ensembl
    4. negative regulation of cytosolic calcium ion concentration Source: Ensembl
    5. negative regulation of heart contraction Source: Ensembl
    6. negative regulation of striated muscle contraction Source: Ensembl
    7. neurotransmitter uptake Source: Ensembl
    8. regulation of blood pressure Source: Ensembl
    9. regulation of respiratory gaseous exchange by neurological system process Source: Ensembl
    10. regulation of smooth muscle contraction Source: Ensembl
    11. regulation of the force of heart contraction Source: Ensembl
    12. regulation of vasoconstriction Source: Ensembl
    13. visual learning Source: Ensembl

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Biological processi

    Ion transport, Potassium transport, Sodium transport, Sodium/potassium transport, Transport

    Keywords - Ligandi

    ATP-binding, Magnesium, Metal-binding, Nucleotide-binding, Potassium, Sodium

    Enzyme and pathway databases

    ReactomeiREACT_209531. Ion transport by P-type ATPases.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Sodium/potassium-transporting ATPase subunit alpha-2 (EC:3.6.3.9)
    Short name:
    Na(+)/K(+) ATPase alpha-2 subunit
    Alternative name(s):
    Sodium pump subunit alpha-2
    Gene namesi
    Name:ATP1A2
    OrganismiSus scrofa (Pig)
    Taxonomic identifieri9823 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaSuinaSuidaeSus
    ProteomesiUP000008227: Chromosome 4

    Subcellular locationi

    Membrane By similarity; Multi-pass membrane protein By similarity. Cell membrane By similarity; Multi-pass membrane protein By similarity

    GO - Cellular componenti

    1. cytoplasm Source: Ensembl
    2. integral component of membrane Source: UniProtKB-KW
    3. T-tubule Source: Ensembl

    Keywords - Cellular componenti

    Cell membrane, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Propeptidei1 – 55By similarityPRO_0000411080
    Chaini6 – 10201015Sodium/potassium-transporting ATPase subunit alpha-2PRO_0000411081Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei570 – 5701PhosphothreonineBy similarity
    Modified residuei587 – 5871PhosphoserineBy similarity
    Modified residuei940 – 9401Phosphoserine; by PKABy similarity

    Keywords - PTMi

    Phosphoprotein

    Interactioni

    Subunit structurei

    Composed of three subunits: alpha (catalytic), beta and gamma.By similarity

    Structurei

    Secondary structure

    1
    1020
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi385 – 3917
    Beta strandi394 – 3974
    Helixi414 – 42512
    Helixi449 – 46214
    Helixi465 – 4706
    Beta strandi474 – 4785
    Beta strandi487 – 4926
    Beta strandi499 – 5068
    Helixi508 – 5136
    Beta strandi515 – 5206
    Beta strandi523 – 5264
    Helixi529 – 54416
    Beta strandi548 – 55710
    Turni559 – 5613
    Turni570 – 5734
    Beta strandi578 – 59013

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1Q3IX-ray2.60A384-590[»]
    ProteinModelPortaliD2WKD8.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiD2WKD8.

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini6 – 8580CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini107 – 12923ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini151 – 286136CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini307 – 31812ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini337 – 769433CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini790 – 79910ExtracellularSequence Analysis
    Topological domaini821 – 84020CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini864 – 91552ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini936 – 94813CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini968 – 98215ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini1004 – 102017CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei86 – 10621HelicalSequence AnalysisAdd
    BLAST
    Transmembranei130 – 15021HelicalSequence AnalysisAdd
    BLAST
    Transmembranei287 – 30620HelicalSequence AnalysisAdd
    BLAST
    Transmembranei319 – 33618HelicalSequence AnalysisAdd
    BLAST
    Transmembranei770 – 78920HelicalSequence AnalysisAdd
    BLAST
    Transmembranei800 – 82021HelicalSequence AnalysisAdd
    BLAST
    Transmembranei841 – 86323HelicalSequence AnalysisAdd
    BLAST
    Transmembranei916 – 93520HelicalSequence AnalysisAdd
    BLAST
    Transmembranei949 – 96719HelicalSequence AnalysisAdd
    BLAST
    Transmembranei983 – 100321HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni80 – 823Interaction with phosphoinositide-3 kinaseBy similarity

    Sequence similaritiesi

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    GeneTreeiENSGT00560000076866.
    KOiK01539.
    OMAiIINIPLP.
    OrthoDBiEOG7327N0.
    TreeFamiTF312838.

    Family and domain databases

    Gene3Di1.20.1110.10. 2 hits.
    2.70.150.10. 2 hits.
    3.40.1110.10. 1 hit.
    InterProiIPR006068. ATPase_P-typ_cation-transptr_C.
    IPR004014. ATPase_P-typ_cation-transptr_N.
    IPR023299. ATPase_P-typ_cyto_domN.
    IPR005775. ATPase_P-typ_Na/K_IIC.
    IPR018303. ATPase_P-typ_P_site.
    IPR023298. ATPase_P-typ_TM_dom.
    IPR008250. ATPase_P-typ_transduc_dom_A.
    IPR001757. Cation_transp_P_typ_ATPase.
    IPR023214. HAD-like_dom.
    [Graphical view]
    PfamiPF00689. Cation_ATPase_C. 1 hit.
    PF00690. Cation_ATPase_N. 1 hit.
    PF00122. E1-E2_ATPase. 1 hit.
    PF00702. Hydrolase. 1 hit.
    [Graphical view]
    PRINTSiPR00119. CATATPASE.
    SMARTiSM00831. Cation_ATPase_N. 1 hit.
    [Graphical view]
    SUPFAMiSSF56784. SSF56784. 2 hits.
    SSF81660. SSF81660. 1 hit.
    TIGRFAMsiTIGR01106. ATPase-IIC_X-K. 1 hit.
    TIGR01494. ATPase_P-type. 2 hits.
    PROSITEiPS00154. ATPASE_E1_E2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    D2WKD8-1 [UniParc]FASTAAdd to Basket

    « Hide

    MGRGAGREYS PAATTAENGG GKKKQKEKEL DELKKEVAMD DHKLSLDELG     50
    RKYQVDLSKG LTNQRAQDIL ARDGPNALTP PPTTPEWVKF CRQLFGGFSI 100
    LLWIGAILCF LAYGIQAAME DEPSNDNLYL GVVLAAVVIV TGCFSYYQEA 150
    KSSKIMDSFK NMVPQQALVV REGEKMQINA EEVVVGDLVE VKGGDRVPAD 200
    LRIISSHGCK VDNSSLTGES EPQTRSPEFT HENPLETRNI CFFSTNCVEG 250
    TARGIVIATG DRTVMGRIAT LASGLEVGRT PIAMEIEHFI QLITGVAVFL 300
    GVSFFVLSLI LGYSWLEAVI FLIGIIVANV PEGLLATVTV CLTLTAKRMA 350
    RKNCLVKNLE AVETLGSTST ICSDKTGTLT QNRMTVAHMW FDNQIHEADT 400
    TEDQSGATFD KRSPTWTALS RIAGLCNRAV FKAGQENISV SKRDTAGDAS 450
    ESALLKCIEL SCGSVRKMRD RNPKVAEIPF NSTNKYQLSI HEREDNPQSH 500
    VLVMKGAPER ILDRCSSILV QGKEIPLDKE MQDAFQNAYL ELGGLGERVL 550
    GFCQLNLPSG KFPRGFKFDT DELNFPTEKL CFVGLMSMID PPRAAVPDAV 600
    GKCRSAGIKV IMVTGDHPIT AKAIAKGVGI ISEGNETVED IAARLNIPVS 650
    QVNPREAKAC VVHGSDLKDM TSEQLDEILK NHTEIVFART SPQQKLIIVE 700
    GCQRQGAIVA VTGDGVNDSP ALKKADIGIA MGIAGSDVSK QAADMILLDD 750
    NFASIVTGVE EGRLIFDNLK KSIAYTLTSN IPEITPFLLF IIANIPLPLG 800
    TVTILCIDLG TDMVPAISLA YEAAESDIMK RQPRNPQTDK LVNERLISMA 850
    YGQIGMIQAL GGFFTYFVIL AENGFLPSRL LGIRLDWDDR SMNDLEDSYG 900
    QEWTYEQRKV VEFTCHTAFF ASIVVVQWAD LIICKTRRNS VFQQGMKNKI 950
    LIFGLLEETA LAAFLSYCPG MGVALRMYPL KVTWWFCAFP YSLLIFIYDE 1000
    VRKLILRRYP GGWVEKETYY 1020
    Length:1,020
    Mass (Da):112,208
    Last modified:March 2, 2010 - v1
    Checksum:i0DFB46632BBA2E2D
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    GQ340776 mRNA. Translation: ADB19854.1.
    CU138477 Genomic DNA. No translation available.
    RefSeqiNP_001165012.1. NM_001171541.1.
    UniGeneiSsc.14403.

    Genome annotation databases

    EnsembliENSSSCT00000007004; ENSSSCP00000006814; ENSSSCG00000006391.
    GeneIDi396828.
    KEGGissc:396828.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    GQ340776 mRNA. Translation: ADB19854.1 .
    CU138477 Genomic DNA. No translation available.
    RefSeqi NP_001165012.1. NM_001171541.1.
    UniGenei Ssc.14403.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1Q3I X-ray 2.60 A 384-590 [» ]
    ProteinModelPortali D2WKD8.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSSSCT00000007004 ; ENSSSCP00000006814 ; ENSSSCG00000006391 .
    GeneIDi 396828.
    KEGGi ssc:396828.

    Organism-specific databases

    CTDi 477.

    Phylogenomic databases

    GeneTreei ENSGT00560000076866.
    KOi K01539.
    OMAi IINIPLP.
    OrthoDBi EOG7327N0.
    TreeFami TF312838.

    Enzyme and pathway databases

    Reactomei REACT_209531. Ion transport by P-type ATPases.

    Miscellaneous databases

    EvolutionaryTracei D2WKD8.

    Family and domain databases

    Gene3Di 1.20.1110.10. 2 hits.
    2.70.150.10. 2 hits.
    3.40.1110.10. 1 hit.
    InterProi IPR006068. ATPase_P-typ_cation-transptr_C.
    IPR004014. ATPase_P-typ_cation-transptr_N.
    IPR023299. ATPase_P-typ_cyto_domN.
    IPR005775. ATPase_P-typ_Na/K_IIC.
    IPR018303. ATPase_P-typ_P_site.
    IPR023298. ATPase_P-typ_TM_dom.
    IPR008250. ATPase_P-typ_transduc_dom_A.
    IPR001757. Cation_transp_P_typ_ATPase.
    IPR023214. HAD-like_dom.
    [Graphical view ]
    Pfami PF00689. Cation_ATPase_C. 1 hit.
    PF00690. Cation_ATPase_N. 1 hit.
    PF00122. E1-E2_ATPase. 1 hit.
    PF00702. Hydrolase. 1 hit.
    [Graphical view ]
    PRINTSi PR00119. CATATPASE.
    SMARTi SM00831. Cation_ATPase_N. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56784. SSF56784. 2 hits.
    SSF81660. SSF81660. 1 hit.
    TIGRFAMsi TIGR01106. ATPase-IIC_X-K. 1 hit.
    TIGR01494. ATPase_P-type. 2 hits.
    PROSITEi PS00154. ATPASE_E1_E2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning of Porcine ATP1A2 mRNA."
      Henriksen C., Bendixen C., Andersen J.P., Vilsen B., Larsen K.
      Submitted (JUL-2009) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. Porcine genome sequencing project
      Submitted (NOV-2009) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    3. "The crystallographic structure of Na,K-ATPase N-domain at 2.6A resolution."
      Hakansson K.O.
      J. Mol. Biol. 332:1175-1182(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.60 ANGSTROMS) OF 384-590.

    Entry informationi

    Entry nameiAT1A2_PIG
    AccessioniPrimary (citable) accession number: D2WKD8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 27, 2011
    Last sequence update: March 2, 2010
    Last modified: October 1, 2014
    This is version 37 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3