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D2WKD8

- AT1A2_PIG

UniProt

D2WKD8 - AT1A2_PIG

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Protein

Sodium/potassium-transporting ATPase subunit alpha-2

Gene

ATP1A2

Organism
Sus scrofa (Pig)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

This is the catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. This action creates the electrochemical gradient of sodium and potassium, providing the energy for active transport of various nutrients (By similarity).By similarity

Catalytic activityi

ATP + H2O + Na+(In) + K+(Out) = ADP + phosphate + Na+(Out) + K+(In).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei374 – 37414-aspartylphosphate intermediateBy similarity
Metal bindingi714 – 7141MagnesiumBy similarity
Metal bindingi718 – 7181MagnesiumBy similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. metal ion binding Source: UniProtKB-KW
  3. sodium:potassium-exchanging ATPase activity Source: UniProtKB-EC

GO - Biological processi

  1. adult locomotory behavior Source: Ensembl
  2. ATP biosynthetic process Source: InterPro
  3. locomotion Source: Ensembl
  4. negative regulation of cytosolic calcium ion concentration Source: Ensembl
  5. negative regulation of heart contraction Source: Ensembl
  6. negative regulation of striated muscle contraction Source: Ensembl
  7. neurotransmitter uptake Source: Ensembl
  8. regulation of blood pressure Source: Ensembl
  9. regulation of respiratory gaseous exchange by neurological system process Source: Ensembl
  10. regulation of smooth muscle contraction Source: Ensembl
  11. regulation of the force of heart contraction Source: Ensembl
  12. regulation of vasoconstriction Source: Ensembl
  13. visual learning Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Ion transport, Potassium transport, Sodium transport, Sodium/potassium transport, Transport

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding, Potassium, Sodium

Enzyme and pathway databases

ReactomeiREACT_209531. Ion transport by P-type ATPases.

Names & Taxonomyi

Protein namesi
Recommended name:
Sodium/potassium-transporting ATPase subunit alpha-2 (EC:3.6.3.9)
Short name:
Na(+)/K(+) ATPase alpha-2 subunit
Alternative name(s):
Sodium pump subunit alpha-2
Gene namesi
Name:ATP1A2
OrganismiSus scrofa (Pig)
Taxonomic identifieri9823 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaSuinaSuidaeSus
ProteomesiUP000008227: Chromosome 4

Subcellular locationi

Membrane By similarity; Multi-pass membrane protein By similarity. Cell membrane By similarity; Multi-pass membrane protein By similarity

GO - Cellular componenti

  1. cytoplasm Source: Ensembl
  2. integral component of membrane Source: UniProtKB-KW
  3. sarcolemma Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Propeptidei1 – 55By similarityPRO_0000411080
Chaini6 – 10201015Sodium/potassium-transporting ATPase subunit alpha-2PRO_0000411081Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei570 – 5701PhosphothreonineBy similarity
Modified residuei587 – 5871PhosphoserineBy similarity
Modified residuei940 – 9401Phosphoserine; by PKABy similarity

Keywords - PTMi

Phosphoprotein

Interactioni

Subunit structurei

Composed of three subunits: alpha (catalytic), beta and gamma.By similarity

Structurei

Secondary structure

1
1020
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi385 – 3917Combined sources
Beta strandi394 – 3974Combined sources
Helixi414 – 42512Combined sources
Helixi449 – 46214Combined sources
Helixi465 – 4706Combined sources
Beta strandi474 – 4785Combined sources
Beta strandi487 – 4926Combined sources
Beta strandi499 – 5068Combined sources
Helixi508 – 5136Combined sources
Beta strandi515 – 5206Combined sources
Beta strandi523 – 5264Combined sources
Helixi529 – 54416Combined sources
Beta strandi548 – 55710Combined sources
Turni559 – 5613Combined sources
Turni570 – 5734Combined sources
Beta strandi578 – 59013Combined sources

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1Q3IX-ray2.60A384-590[»]
ProteinModelPortaliD2WKD8.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiD2WKD8.

Topological domain

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini6 – 8580CytoplasmicSequence AnalysisAdd
BLAST
Topological domaini107 – 12923ExtracellularSequence AnalysisAdd
BLAST
Topological domaini151 – 286136CytoplasmicSequence AnalysisAdd
BLAST
Topological domaini307 – 31812ExtracellularSequence AnalysisAdd
BLAST
Topological domaini337 – 769433CytoplasmicSequence AnalysisAdd
BLAST
Topological domaini790 – 79910ExtracellularSequence Analysis
Topological domaini821 – 84020CytoplasmicSequence AnalysisAdd
BLAST
Topological domaini864 – 91552ExtracellularSequence AnalysisAdd
BLAST
Topological domaini936 – 94813CytoplasmicSequence AnalysisAdd
BLAST
Topological domaini968 – 98215ExtracellularSequence AnalysisAdd
BLAST
Topological domaini1004 – 102017CytoplasmicSequence AnalysisAdd
BLAST

Transmembrane

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei86 – 10621HelicalSequence AnalysisAdd
BLAST
Transmembranei130 – 15021HelicalSequence AnalysisAdd
BLAST
Transmembranei287 – 30620HelicalSequence AnalysisAdd
BLAST
Transmembranei319 – 33618HelicalSequence AnalysisAdd
BLAST
Transmembranei770 – 78920HelicalSequence AnalysisAdd
BLAST
Transmembranei800 – 82021HelicalSequence AnalysisAdd
BLAST
Transmembranei841 – 86323HelicalSequence AnalysisAdd
BLAST
Transmembranei916 – 93520HelicalSequence AnalysisAdd
BLAST
Transmembranei949 – 96719HelicalSequence AnalysisAdd
BLAST
Transmembranei983 – 100321HelicalSequence AnalysisAdd
BLAST

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni80 – 823Interaction with phosphoinositide-3 kinaseBy similarity

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00760000119003.
InParanoidiD2WKD8.
KOiK01539.
OMAiIINIPLP.
OrthoDBiEOG7327N0.
TreeFamiTF312838.

Family and domain databases

Gene3Di1.20.1110.10. 2 hits.
2.70.150.10. 2 hits.
3.40.1110.10. 1 hit.
InterProiIPR006068. ATPase_P-typ_cation-transptr_C.
IPR004014. ATPase_P-typ_cation-transptr_N.
IPR023299. ATPase_P-typ_cyto_domN.
IPR005775. ATPase_P-typ_Na/K_IIC.
IPR018303. ATPase_P-typ_P_site.
IPR023298. ATPase_P-typ_TM_dom.
IPR008250. ATPase_P-typ_transduc_dom_A.
IPR001757. Cation_transp_P_typ_ATPase.
IPR023214. HAD-like_dom.
[Graphical view]
PfamiPF00689. Cation_ATPase_C. 1 hit.
PF00690. Cation_ATPase_N. 1 hit.
PF00122. E1-E2_ATPase. 1 hit.
PF00702. Hydrolase. 1 hit.
[Graphical view]
PRINTSiPR00119. CATATPASE.
SMARTiSM00831. Cation_ATPase_N. 1 hit.
[Graphical view]
SUPFAMiSSF56784. SSF56784. 2 hits.
SSF81660. SSF81660. 1 hit.
TIGRFAMsiTIGR01106. ATPase-IIC_X-K. 1 hit.
TIGR01494. ATPase_P-type. 2 hits.
PROSITEiPS00154. ATPASE_E1_E2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

D2WKD8-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MGRGAGREYS PAATTAENGG GKKKQKEKEL DELKKEVAMD DHKLSLDELG
60 70 80 90 100
RKYQVDLSKG LTNQRAQDIL ARDGPNALTP PPTTPEWVKF CRQLFGGFSI
110 120 130 140 150
LLWIGAILCF LAYGIQAAME DEPSNDNLYL GVVLAAVVIV TGCFSYYQEA
160 170 180 190 200
KSSKIMDSFK NMVPQQALVV REGEKMQINA EEVVVGDLVE VKGGDRVPAD
210 220 230 240 250
LRIISSHGCK VDNSSLTGES EPQTRSPEFT HENPLETRNI CFFSTNCVEG
260 270 280 290 300
TARGIVIATG DRTVMGRIAT LASGLEVGRT PIAMEIEHFI QLITGVAVFL
310 320 330 340 350
GVSFFVLSLI LGYSWLEAVI FLIGIIVANV PEGLLATVTV CLTLTAKRMA
360 370 380 390 400
RKNCLVKNLE AVETLGSTST ICSDKTGTLT QNRMTVAHMW FDNQIHEADT
410 420 430 440 450
TEDQSGATFD KRSPTWTALS RIAGLCNRAV FKAGQENISV SKRDTAGDAS
460 470 480 490 500
ESALLKCIEL SCGSVRKMRD RNPKVAEIPF NSTNKYQLSI HEREDNPQSH
510 520 530 540 550
VLVMKGAPER ILDRCSSILV QGKEIPLDKE MQDAFQNAYL ELGGLGERVL
560 570 580 590 600
GFCQLNLPSG KFPRGFKFDT DELNFPTEKL CFVGLMSMID PPRAAVPDAV
610 620 630 640 650
GKCRSAGIKV IMVTGDHPIT AKAIAKGVGI ISEGNETVED IAARLNIPVS
660 670 680 690 700
QVNPREAKAC VVHGSDLKDM TSEQLDEILK NHTEIVFART SPQQKLIIVE
710 720 730 740 750
GCQRQGAIVA VTGDGVNDSP ALKKADIGIA MGIAGSDVSK QAADMILLDD
760 770 780 790 800
NFASIVTGVE EGRLIFDNLK KSIAYTLTSN IPEITPFLLF IIANIPLPLG
810 820 830 840 850
TVTILCIDLG TDMVPAISLA YEAAESDIMK RQPRNPQTDK LVNERLISMA
860 870 880 890 900
YGQIGMIQAL GGFFTYFVIL AENGFLPSRL LGIRLDWDDR SMNDLEDSYG
910 920 930 940 950
QEWTYEQRKV VEFTCHTAFF ASIVVVQWAD LIICKTRRNS VFQQGMKNKI
960 970 980 990 1000
LIFGLLEETA LAAFLSYCPG MGVALRMYPL KVTWWFCAFP YSLLIFIYDE
1010 1020
VRKLILRRYP GGWVEKETYY
Length:1,020
Mass (Da):112,208
Last modified:March 2, 2010 - v1
Checksum:i0DFB46632BBA2E2D
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
GQ340776 mRNA. Translation: ADB19854.1.
CU138477 Genomic DNA. No translation available.
RefSeqiNP_001165012.1. NM_001171541.1.
UniGeneiSsc.14403.

Genome annotation databases

EnsembliENSSSCT00000007004; ENSSSCP00000006814; ENSSSCG00000006391.
GeneIDi396828.
KEGGissc:396828.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
GQ340776 mRNA. Translation: ADB19854.1 .
CU138477 Genomic DNA. No translation available.
RefSeqi NP_001165012.1. NM_001171541.1.
UniGenei Ssc.14403.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1Q3I X-ray 2.60 A 384-590 [» ]
ProteinModelPortali D2WKD8.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSSSCT00000007004 ; ENSSSCP00000006814 ; ENSSSCG00000006391 .
GeneIDi 396828.
KEGGi ssc:396828.

Organism-specific databases

CTDi 477.

Phylogenomic databases

GeneTreei ENSGT00760000119003.
InParanoidi D2WKD8.
KOi K01539.
OMAi IINIPLP.
OrthoDBi EOG7327N0.
TreeFami TF312838.

Enzyme and pathway databases

Reactomei REACT_209531. Ion transport by P-type ATPases.

Miscellaneous databases

EvolutionaryTracei D2WKD8.

Family and domain databases

Gene3Di 1.20.1110.10. 2 hits.
2.70.150.10. 2 hits.
3.40.1110.10. 1 hit.
InterProi IPR006068. ATPase_P-typ_cation-transptr_C.
IPR004014. ATPase_P-typ_cation-transptr_N.
IPR023299. ATPase_P-typ_cyto_domN.
IPR005775. ATPase_P-typ_Na/K_IIC.
IPR018303. ATPase_P-typ_P_site.
IPR023298. ATPase_P-typ_TM_dom.
IPR008250. ATPase_P-typ_transduc_dom_A.
IPR001757. Cation_transp_P_typ_ATPase.
IPR023214. HAD-like_dom.
[Graphical view ]
Pfami PF00689. Cation_ATPase_C. 1 hit.
PF00690. Cation_ATPase_N. 1 hit.
PF00122. E1-E2_ATPase. 1 hit.
PF00702. Hydrolase. 1 hit.
[Graphical view ]
PRINTSi PR00119. CATATPASE.
SMARTi SM00831. Cation_ATPase_N. 1 hit.
[Graphical view ]
SUPFAMi SSF56784. SSF56784. 2 hits.
SSF81660. SSF81660. 1 hit.
TIGRFAMsi TIGR01106. ATPase-IIC_X-K. 1 hit.
TIGR01494. ATPase_P-type. 2 hits.
PROSITEi PS00154. ATPASE_E1_E2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning of Porcine ATP1A2 mRNA."
    Henriksen C., Bendixen C., Andersen J.P., Vilsen B., Larsen K.
    Submitted (JUL-2009) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. Porcine genome sequencing project
    Submitted (NOV-2009) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  3. "The crystallographic structure of Na,K-ATPase N-domain at 2.6A resolution."
    Hakansson K.O.
    J. Mol. Biol. 332:1175-1182(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.60 ANGSTROMS) OF 384-590.

Entry informationi

Entry nameiAT1A2_PIG
AccessioniPrimary (citable) accession number: D2WKD8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 27, 2011
Last sequence update: March 2, 2010
Last modified: October 29, 2014
This is version 38 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3