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Protein

Sodium/potassium-transporting ATPase subunit alpha-2

Gene

ATP1A2

Organism
Sus scrofa (Pig)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

This is the catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. This action creates the electrochemical gradient of sodium and potassium, providing the energy for active transport of various nutrients (By similarity).By similarity

Catalytic activityi

ATP + H2O + Na+(In) + K+(Out) = ADP + phosphate + Na+(Out) + K+(In).

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei3744-aspartylphosphate intermediateBy similarity1
Metal bindingi714MagnesiumBy similarity1
Metal bindingi718MagnesiumBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Ion transport, Potassium transport, Sodium transport, Sodium/potassium transport, Transport

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding, Potassium, Sodium

Enzyme and pathway databases

ReactomeiR-SSC-5578775. Ion homeostasis.
R-SSC-936837. Ion transport by P-type ATPases.

Names & Taxonomyi

Protein namesi
Recommended name:
Sodium/potassium-transporting ATPase subunit alpha-2 (EC:3.6.3.9)
Short name:
Na(+)/K(+) ATPase alpha-2 subunit
Alternative name(s):
Sodium pump subunit alpha-2
Gene namesi
Name:ATP1A2
OrganismiSus scrofa (Pig)
Taxonomic identifieri9823 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaSuinaSuidaeSus
Proteomesi
  • UP000008227 Componenti: Chromosome 4

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini6 – 85CytoplasmicSequence analysisAdd BLAST80
Transmembranei86 – 106HelicalSequence analysisAdd BLAST21
Topological domaini107 – 129ExtracellularSequence analysisAdd BLAST23
Transmembranei130 – 150HelicalSequence analysisAdd BLAST21
Topological domaini151 – 286CytoplasmicSequence analysisAdd BLAST136
Transmembranei287 – 306HelicalSequence analysisAdd BLAST20
Topological domaini307 – 318ExtracellularSequence analysisAdd BLAST12
Transmembranei319 – 336HelicalSequence analysisAdd BLAST18
Topological domaini337 – 769CytoplasmicSequence analysisAdd BLAST433
Transmembranei770 – 789HelicalSequence analysisAdd BLAST20
Topological domaini790 – 799ExtracellularSequence analysis10
Transmembranei800 – 820HelicalSequence analysisAdd BLAST21
Topological domaini821 – 840CytoplasmicSequence analysisAdd BLAST20
Transmembranei841 – 863HelicalSequence analysisAdd BLAST23
Topological domaini864 – 915ExtracellularSequence analysisAdd BLAST52
Transmembranei916 – 935HelicalSequence analysisAdd BLAST20
Topological domaini936 – 948CytoplasmicSequence analysisAdd BLAST13
Transmembranei949 – 967HelicalSequence analysisAdd BLAST19
Topological domaini968 – 982ExtracellularSequence analysisAdd BLAST15
Transmembranei983 – 1003HelicalSequence analysisAdd BLAST21
Topological domaini1004 – 1020CytoplasmicSequence analysisAdd BLAST17

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
PropeptideiPRO_00004110802 – 5By similarity4
ChainiPRO_00004110816 – 1020Sodium/potassium-transporting ATPase subunit alpha-2Add BLAST1015

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei10PhosphoserineBy similarity1
Modified residuei45PhosphoserineBy similarity1
Modified residuei226PhosphoserineBy similarity1
Modified residuei273PhosphoserineBy similarity1
Modified residuei439PhosphoserineBy similarity1
Modified residuei450PhosphoserineBy similarity1
Modified residuei559PhosphoserineBy similarity1
Modified residuei570PhosphothreonineBy similarity1
Modified residuei587PhosphoserineBy similarity1
Modified residuei672PhosphoserineBy similarity1
Modified residuei826PhosphoserineBy similarity1
Modified residuei940Phosphoserine; by PKABy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiD2WKD8.
PeptideAtlasiD2WKD8.
PRIDEiD2WKD8.

Expressioni

Gene expression databases

BgeeiENSSSCG00000006391.
GenevisibleiD2WKD8. SS.

Interactioni

Subunit structurei

Composed of three subunits: alpha (catalytic), beta and gamma.By similarity

Protein-protein interaction databases

STRINGi9823.ENSSSCP00000006814.

Structurei

Secondary structure

11020
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi385 – 391Combined sources7
Beta strandi394 – 397Combined sources4
Helixi414 – 425Combined sources12
Helixi449 – 462Combined sources14
Helixi465 – 470Combined sources6
Beta strandi474 – 478Combined sources5
Beta strandi487 – 492Combined sources6
Beta strandi499 – 506Combined sources8
Helixi508 – 513Combined sources6
Beta strandi515 – 520Combined sources6
Beta strandi523 – 526Combined sources4
Helixi529 – 544Combined sources16
Beta strandi548 – 557Combined sources10
Turni559 – 561Combined sources3
Turni570 – 573Combined sources4
Beta strandi578 – 590Combined sources13

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1Q3IX-ray2.60A384-590[»]
ProteinModelPortaliD2WKD8.
SMRiD2WKD8.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiD2WKD8.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni80 – 82Interaction with phosphoinositide-3 kinaseBy similarity3

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0203. Eukaryota.
COG0474. LUCA.
GeneTreeiENSGT00850000132256.
InParanoidiD2WKD8.
KOiK01539.
OMAiMREAFNN.
OrthoDBiEOG091G01BB.
TreeFamiTF312838.

Family and domain databases

Gene3Di1.20.1110.10. 2 hits.
2.70.150.10. 2 hits.
3.40.1110.10. 1 hit.
InterProiIPR006068. ATPase_P-typ_cation-transptr_C.
IPR004014. ATPase_P-typ_cation-transptr_N.
IPR023299. ATPase_P-typ_cyto_domN.
IPR018303. ATPase_P-typ_P_site.
IPR023298. ATPase_P-typ_TM_dom.
IPR008250. ATPase_P-typ_transduc_dom_A.
IPR023214. HAD-like_dom.
IPR005775. P-type_ATPase_IIC.
IPR001757. P_typ_ATPase.
[Graphical view]
PfamiPF00689. Cation_ATPase_C. 1 hit.
PF00690. Cation_ATPase_N. 1 hit.
PF00122. E1-E2_ATPase. 1 hit.
[Graphical view]
SMARTiSM00831. Cation_ATPase_N. 1 hit.
[Graphical view]
SUPFAMiSSF56784. SSF56784. 2 hits.
SSF81660. SSF81660. 1 hit.
TIGRFAMsiTIGR01106. ATPase-IIC_X-K. 1 hit.
TIGR01494. ATPase_P-type. 2 hits.
PROSITEiPS00154. ATPASE_E1_E2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

D2WKD8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGRGAGREYS PAATTAENGG GKKKQKEKEL DELKKEVAMD DHKLSLDELG
60 70 80 90 100
RKYQVDLSKG LTNQRAQDIL ARDGPNALTP PPTTPEWVKF CRQLFGGFSI
110 120 130 140 150
LLWIGAILCF LAYGIQAAME DEPSNDNLYL GVVLAAVVIV TGCFSYYQEA
160 170 180 190 200
KSSKIMDSFK NMVPQQALVV REGEKMQINA EEVVVGDLVE VKGGDRVPAD
210 220 230 240 250
LRIISSHGCK VDNSSLTGES EPQTRSPEFT HENPLETRNI CFFSTNCVEG
260 270 280 290 300
TARGIVIATG DRTVMGRIAT LASGLEVGRT PIAMEIEHFI QLITGVAVFL
310 320 330 340 350
GVSFFVLSLI LGYSWLEAVI FLIGIIVANV PEGLLATVTV CLTLTAKRMA
360 370 380 390 400
RKNCLVKNLE AVETLGSTST ICSDKTGTLT QNRMTVAHMW FDNQIHEADT
410 420 430 440 450
TEDQSGATFD KRSPTWTALS RIAGLCNRAV FKAGQENISV SKRDTAGDAS
460 470 480 490 500
ESALLKCIEL SCGSVRKMRD RNPKVAEIPF NSTNKYQLSI HEREDNPQSH
510 520 530 540 550
VLVMKGAPER ILDRCSSILV QGKEIPLDKE MQDAFQNAYL ELGGLGERVL
560 570 580 590 600
GFCQLNLPSG KFPRGFKFDT DELNFPTEKL CFVGLMSMID PPRAAVPDAV
610 620 630 640 650
GKCRSAGIKV IMVTGDHPIT AKAIAKGVGI ISEGNETVED IAARLNIPVS
660 670 680 690 700
QVNPREAKAC VVHGSDLKDM TSEQLDEILK NHTEIVFART SPQQKLIIVE
710 720 730 740 750
GCQRQGAIVA VTGDGVNDSP ALKKADIGIA MGIAGSDVSK QAADMILLDD
760 770 780 790 800
NFASIVTGVE EGRLIFDNLK KSIAYTLTSN IPEITPFLLF IIANIPLPLG
810 820 830 840 850
TVTILCIDLG TDMVPAISLA YEAAESDIMK RQPRNPQTDK LVNERLISMA
860 870 880 890 900
YGQIGMIQAL GGFFTYFVIL AENGFLPSRL LGIRLDWDDR SMNDLEDSYG
910 920 930 940 950
QEWTYEQRKV VEFTCHTAFF ASIVVVQWAD LIICKTRRNS VFQQGMKNKI
960 970 980 990 1000
LIFGLLEETA LAAFLSYCPG MGVALRMYPL KVTWWFCAFP YSLLIFIYDE
1010 1020
VRKLILRRYP GGWVEKETYY
Length:1,020
Mass (Da):112,208
Last modified:March 2, 2010 - v1
Checksum:i0DFB46632BBA2E2D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
GQ340776 mRNA. Translation: ADB19854.1.
CU138477 Genomic DNA. No translation available.
RefSeqiNP_001165012.1. NM_001171541.1.
XP_013852329.1. XM_013996875.1.
UniGeneiSsc.14403.

Genome annotation databases

EnsembliENSSSCT00000007004; ENSSSCP00000006814; ENSSSCG00000006391.
GeneIDi396828.
KEGGissc:396828.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
GQ340776 mRNA. Translation: ADB19854.1.
CU138477 Genomic DNA. No translation available.
RefSeqiNP_001165012.1. NM_001171541.1.
XP_013852329.1. XM_013996875.1.
UniGeneiSsc.14403.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1Q3IX-ray2.60A384-590[»]
ProteinModelPortaliD2WKD8.
SMRiD2WKD8.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9823.ENSSSCP00000006814.

Proteomic databases

PaxDbiD2WKD8.
PeptideAtlasiD2WKD8.
PRIDEiD2WKD8.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSSSCT00000007004; ENSSSCP00000006814; ENSSSCG00000006391.
GeneIDi396828.
KEGGissc:396828.

Organism-specific databases

CTDi477.

Phylogenomic databases

eggNOGiKOG0203. Eukaryota.
COG0474. LUCA.
GeneTreeiENSGT00850000132256.
InParanoidiD2WKD8.
KOiK01539.
OMAiMREAFNN.
OrthoDBiEOG091G01BB.
TreeFamiTF312838.

Enzyme and pathway databases

ReactomeiR-SSC-5578775. Ion homeostasis.
R-SSC-936837. Ion transport by P-type ATPases.

Miscellaneous databases

EvolutionaryTraceiD2WKD8.

Gene expression databases

BgeeiENSSSCG00000006391.
GenevisibleiD2WKD8. SS.

Family and domain databases

Gene3Di1.20.1110.10. 2 hits.
2.70.150.10. 2 hits.
3.40.1110.10. 1 hit.
InterProiIPR006068. ATPase_P-typ_cation-transptr_C.
IPR004014. ATPase_P-typ_cation-transptr_N.
IPR023299. ATPase_P-typ_cyto_domN.
IPR018303. ATPase_P-typ_P_site.
IPR023298. ATPase_P-typ_TM_dom.
IPR008250. ATPase_P-typ_transduc_dom_A.
IPR023214. HAD-like_dom.
IPR005775. P-type_ATPase_IIC.
IPR001757. P_typ_ATPase.
[Graphical view]
PfamiPF00689. Cation_ATPase_C. 1 hit.
PF00690. Cation_ATPase_N. 1 hit.
PF00122. E1-E2_ATPase. 1 hit.
[Graphical view]
SMARTiSM00831. Cation_ATPase_N. 1 hit.
[Graphical view]
SUPFAMiSSF56784. SSF56784. 2 hits.
SSF81660. SSF81660. 1 hit.
TIGRFAMsiTIGR01106. ATPase-IIC_X-K. 1 hit.
TIGR01494. ATPase_P-type. 2 hits.
PROSITEiPS00154. ATPASE_E1_E2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiAT1A2_PIG
AccessioniPrimary (citable) accession number: D2WKD8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 27, 2011
Last sequence update: March 2, 2010
Last modified: November 2, 2016
This is version 55 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.