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D2UGX8 (D2UGX8_XANAP) Unreviewed, UniProtKB/TrEMBL

Last modified May 1, 2013. Version 26. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Formamidopyrimidine-DNA glycosylase HAMAP-Rule MF_00103
Short name=Fapy-DNA glycosylase HAMAP-Rule MF_00103
EC=3.2.2.23 HAMAP-Rule MF_00103
Alternative name(s):
DNA-(apurinic or apyrimidinic site) lyase MutM HAMAP-Rule MF_00103
Gene names
Name:mutM HAMAP-Rule MF_00103 EMBL CBA17639.1
Synonyms:fpg HAMAP-Rule MF_00103
Ordered Locus Names:XALC_3160 EMBL CBA17639.1, XALc_3160
OrganismXanthomonas albilineans (strain GPE PC73 / CFBP 7063) [Complete proteome] [HAMAP] EMBL CBA17639.1
Taxonomic identifier380358 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Gammaproteobacteria › Xanthomonadales › Xanthomonadaceae › Xanthomonas ›

Protein attributes

Sequence length270 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Involved in base excision repair of DNA damaged by oxidation or by mutagenic agents. Acts as DNA glycosylase that recognizes and removes damaged bases. Has a preference for oxidized purines, such as 7,8-dihydro-8-oxoguanine (8-oxoG). Has AP (apurinic/apyrimidinic) lyase activity and introduces nicks in the DNA strand. Cleaves the DNA backbone by beta-delta elimination to generate a single-strand break at the site of the removed base with both 3'- and 5'-phosphates By similarity. HAMAP-Rule MF_00103 SAAS SAAS015886

Catalytic activity

Hydrolysis of DNA containing ring-opened 7-methylguanine residues, releasing 2,6-diamino-4-hydroxy-5-(N-methyl)formamidopyrimidine. HAMAP-Rule MF_00103 SAAS SAAS015886

The C-O-P bond 3' to the apurinic or apyrimidinic site in DNA is broken by a beta-elimination reaction, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate. HAMAP-Rule MF_00103 SAAS SAAS015886

Cofactor

Binds 1 zinc ion per subunit By similarity. HAMAP-Rule MF_00103 SAAS SAAS015886

Subunit structure

Monomer By similarity. HAMAP-Rule MF_00103 SAAS SAAS015886

Sequence similarities

Belongs to the FPG family. HAMAP-Rule MF_00103

Contains 1 FPG-type zinc finger. HAMAP-Rule MF_00103 SAAS SAAS015886

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Regions

Zinc finger236 – 27035FPG-type By similarity HAMAP-Rule MF_00103

Sites

Active site21Schiff-base intermediate with DNA By similarity HAMAP-Rule MF_00103
Active site31Proton donor By similarity HAMAP-Rule MF_00103
Active site581Proton donor; for beta-elimination activity By similarity HAMAP-Rule MF_00103
Active site2601Proton donor; for delta-elimination activity By similarity HAMAP-Rule MF_00103
Binding site911DNA By similarity HAMAP-Rule MF_00103
Binding site1101DNA By similarity HAMAP-Rule MF_00103
Binding site1511DNA By similarity HAMAP-Rule MF_00103

Sequences

Sequence LengthMass (Da)Tools
D2UGX8 [UniParc].

Last modified March 2, 2010. Version 1.
Checksum: 5342FB56BD265CF3

FASTA27029,850
        10         20         30         40         50         60 
MPELPEVETT RRGLEPHLAG RRIHGVILRR PDLRWPIPPE VTQQLPGQRI AGIHRRAKYL 

        70         80         90        100        110        120 
LLETAAGSAL LHLGMSGSLR VLPGDTPPRT HDHVDMSLED GRVLRFNDPR RFGCLLWQAP 

       130        140        150        160        170        180 
GTLHPLLAEL GPEPLSDAFD GDYLFQLSRG RKAPVKTFLM DQRIVVGVGN IYAAESLFQA 

       190        200        210        220        230        240 
GISPLRAAGE VSRARYARLA EATKAILGYA IQRGGTTLRD FISPDGAPGY FEQELSVYGR 

       250        260        270 
EGEACKRCGR LLRHASIGQR ASVWCGHCQR

« Hide

References

[1]"The complete genome sequence of Xanthomonas albilineans provides new insights into the reductive genome evolution of the xylem-limited Xanthomonadaceae."
Pieretti I., Royer M., Barbe V., Carrere S., Koebnik R., Cociancich S., Couloux A., Darrasse A., Gouzy J., Jacques M.A., Lauber E., Manceau C., Mangenot S., Poussier S., Segurens B., Szurek B., Verdier V., Arlat M., Rott P.
BMC Genomics 10:616-616(2009)
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: GPE PC73 / CFBP 7063.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		FP565176 Genomic DNA. Translation: CBA17639.1.
RefSeqYP_003377633.1. NC_013722.1.

3D structure databases

ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID8702337.
KEGGxal:XALc_3160.
PATRIC32548843. VBIXanAlb89132_3141.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMHOG000020881.
KOK10563.

Family and domain databases

HAMAPMF_00103. Fapy_DNA_glycosyl.
InterProIPR015886. DNA_glyclase/AP_lyase_DNA-bd.
IPR015887. DNA_glyclase_Znf_dom_DNA_BS.
IPR000191. DNA_glycosylase/AP_lyase.
IPR012319. DNA_glycosylase/AP_lyase_cat.
IPR020629. Formamido-pyr_DNA_Glyclase.
IPR010979. Ribosomal_S13-like_H2TH.
IPR000214. Znf_DNA_glyclase/AP_lyase.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PfamPF01149. Fapy_DNA_glyco. 1 hit.
PF06831. H2TH. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
SMARTSM00898. Fapy_DNA_glyco. 1 hit.
[Graphical view]
SUPFAMSSF81624. Form_DNAglyc_cat. 1 hit.
SSF46946. Ribosomal_H2TH. 1 hit.
TIGRFAMsTIGR00577. fpg. 1 hit.
PROSITEPS51068. FPG_CAT. 1 hit.
PS01242. ZF_FPG_1. 1 hit.
PS51066. ZF_FPG_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameD2UGX8_XANAP
AccessionPrimary (citable) accession number: D2UGX8
Entry history
Integrated into UniProtKB/TrEMBL: March 2, 2010
Last sequence update: March 2, 2010
Last modified: May 1, 2013
This is version 26 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info