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D2TRT2

- APHA_CITRI

UniProt

D2TRT2 - APHA_CITRI

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Protein

Class B acid phosphatase

Gene

aphA

Organism
Citrobacter rodentium (strain ICC168) (Citrobacter freundii biotype 4280)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Dephosphorylates several organic phosphate monoesters. Also has a phosphotransferase activity catalyzing the transfer of low-energy phosphate groups from organic phosphate monoesters to free hydroxyl groups of various organic compounds (By similarity).By similarity

Catalytic activityi

A phosphate monoester + H2O = an alcohol + phosphate.By similarity

Cofactori

Binds 1 magnesium ion per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei69 – 691NucleophileBy similarity
Metal bindingi69 – 691MagnesiumBy similarity
Active sitei71 – 711Proton donorBy similarity
Metal bindingi71 – 711Magnesium; via carbonyl oxygenBy similarity
Binding sitei177 – 1771SubstrateBy similarity
Metal bindingi192 – 1921MagnesiumBy similarity

GO - Molecular functioni

  1. acid phosphatase activity Source: UniProtKB-EC
  2. metal ion binding Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

BioCyciCROD637910:GJIG-3659-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Class B acid phosphataseImportedBy similarity (EC:3.1.3.2ImportedBy similarity)
Short name:
CBAPBy similarity
Gene namesi
Name:aphAImportedBy similarity
Ordered Locus Names:ROD_36361
OrganismiCitrobacter rodentium (strain ICC168) (Citrobacter freundii biotype 4280)
Taxonomic identifieri637910 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeCitrobacter
ProteomesiUP000001889: Chromosome

Subcellular locationi

Periplasm By similarity

GO - Cellular componenti

  1. outer membrane-bounded periplasmic space Source: InterPro
Complete GO annotation...

Keywords - Cellular componenti

Periplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2525ImportedSequence AnalysisAdd
BLAST
Chaini26 – 237212Class B acid phosphataseSequence AnalysisPRO_5000566034Add
BLAST

Interactioni

Subunit structurei

Homotetramer.By similarity

Structurei

3D structure databases

ProteinModelPortaliD2TRT2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni137 – 1382Substrate bindingBy similarity

Sequence similaritiesi

Belongs to the class B bacterial acid phosphatase family.By similarity

Keywords - Domaini

Signal

Phylogenomic databases

HOGENOMiHOG000270623.
KOiK03788.
OMAiPEFWEKM.

Family and domain databases

Gene3Di3.40.50.1000. 1 hit.
InterProiIPR005519. Acid_phosphat_B.
IPR023214. HAD-like_dom.
IPR010025. HAD-SF_ppase_IIIB_AphA.
[Graphical view]
PfamiPF03767. Acid_phosphat_B. 1 hit.
[Graphical view]
PIRSFiPIRSF017818. Acid_Ptase_B. 1 hit.
SUPFAMiSSF56784. SSF56784. 1 hit.
TIGRFAMsiTIGR01672. AphA. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

D2TRT2-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MRKVSLALSA ACLLFTLNYT ASALASSPSP LNPGTNVAKL AEQAPVHWVS
60 70 80 90 100
VAQIEKSLTG RPPMAVGFDI DDTVLFSSPG FWRGKKMYSP DSEEYLKNPA
110 120 130 140 150
FWEKMNNGWD EFSIPKEVAR QLIALHLRRG DSIFFVTGRS QTSNERVSKT
160 170 180 190 200
LADDFLIPAT VMNPVIFAGD KPGQNSKIQW LQAKNIRIFY GDSDNDIAAA
210 220 230
RELGIRGIRI LRASNSTYKP LPQAGAFGEE VIVNSEY
Length:237
Mass (Da):26,157
Last modified:March 2, 2010 - v1
Checksum:i3BF9EA0904C88479
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
FN543502 Genomic DNA. Translation: CBG90344.1.
RefSeqiWP_012907668.1. NC_013716.1.
YP_003367081.1. NC_013716.1.

Genome annotation databases

EnsemblBacteriaiCBG90344; CBG90344; ROD_36361.
GeneIDi8711915.
KEGGicro:ROD_36361.
PATRICi32030966. VBICitRod33214_3468.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
FN543502 Genomic DNA. Translation: CBG90344.1 .
RefSeqi WP_012907668.1. NC_013716.1.
YP_003367081.1. NC_013716.1.

3D structure databases

ProteinModelPortali D2TRT2.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CBG90344 ; CBG90344 ; ROD_36361 .
GeneIDi 8711915.
KEGGi cro:ROD_36361.
PATRICi 32030966. VBICitRod33214_3468.

Phylogenomic databases

HOGENOMi HOG000270623.
KOi K03788.
OMAi PEFWEKM.

Enzyme and pathway databases

BioCyci CROD637910:GJIG-3659-MONOMER.

Family and domain databases

Gene3Di 3.40.50.1000. 1 hit.
InterProi IPR005519. Acid_phosphat_B.
IPR023214. HAD-like_dom.
IPR010025. HAD-SF_ppase_IIIB_AphA.
[Graphical view ]
Pfami PF03767. Acid_phosphat_B. 1 hit.
[Graphical view ]
PIRSFi PIRSF017818. Acid_Ptase_B. 1 hit.
SUPFAMi SSF56784. SSF56784. 1 hit.
TIGRFAMsi TIGR01672. AphA. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "The Citrobacter rodentium genome sequence reveals convergent evolution with human pathogenic Escherichia coli."
    Petty N.K., Bulgin R., Crepin V.F., Cerdeno-Tarraga A.M., Schroeder G.N., Quail M.A., Lennard N., Corton C., Barron A., Clark L., Toribio A.L., Parkhill J., Dougan G., Frankel G., Thomson N.R.
    J. Bacteriol. 192:525-538(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ICC1681 Publication.

Entry informationi

Entry nameiAPHA_CITRI
AccessioniPrimary (citable) accession number: D2TRT2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 25, 2012
Last sequence update: March 2, 2010
Last modified: October 29, 2014
This is version 28 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3