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D2TRT2 (APHA_CITRI) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 26. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Class B acid phosphatase

Short name=CBAP
EC=3.1.3.2
Gene names
Name:aphA
Ordered Locus Names:ROD_36361
OrganismCitrobacter rodentium (strain ICC168) (Citrobacter freundii biotype 4280) [Complete proteome] [HAMAP]
Taxonomic identifier637910 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeCitrobacter

Protein attributes

Sequence length237 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceInferred from homology

General annotation (Comments)

Function

Dephosphorylates several organic phosphate monoesters. Also has a phosphotransferase activity catalyzing the transfer of low-energy phosphate groups from organic phosphate monoesters to free hydroxyl groups of various organic compounds By similarity.

Catalytic activity

A phosphate monoester + H2O = an alcohol + phosphate.

Cofactor

Binds 1 magnesium ion per subunit By similarity.

Subunit structure

Homotetramer By similarity.

Subcellular location

Periplasm By similarity.

Sequence similarities

Belongs to the class B bacterial acid phosphatase family.

Ontologies

Keywords
   Cellular componentPeriplasm
   DomainSignal
   LigandMagnesium
Metal-binding
   Molecular functionHydrolase
   Technical termComplete proteome
Gene Ontology (GO)
   Cellular_componentouter membrane-bounded periplasmic space

Inferred from electronic annotation. Source: InterPro

   Molecular_functionacid phosphatase activity

Inferred from electronic annotation. Source: UniProtKB-EC

metal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2525 Potential
Chain26 – 237212Class B acid phosphatase
PRO_5000566034

Regions

Region137 – 1382Substrate binding By similarity

Sites

Active site691Nucleophile By similarity
Active site711Proton donor By similarity
Metal binding691Magnesium By similarity
Metal binding711Magnesium; via carbonyl oxygen By similarity
Metal binding1921Magnesium By similarity
Binding site1771Substrate By similarity

Sequences

Sequence LengthMass (Da)Tools
D2TRT2 [UniParc].

Last modified March 2, 2010. Version 1.
Checksum: 3BF9EA0904C88479

FASTA23726,157
        10         20         30         40         50         60 
MRKVSLALSA ACLLFTLNYT ASALASSPSP LNPGTNVAKL AEQAPVHWVS VAQIEKSLTG 

        70         80         90        100        110        120 
RPPMAVGFDI DDTVLFSSPG FWRGKKMYSP DSEEYLKNPA FWEKMNNGWD EFSIPKEVAR 

       130        140        150        160        170        180 
QLIALHLRRG DSIFFVTGRS QTSNERVSKT LADDFLIPAT VMNPVIFAGD KPGQNSKIQW 

       190        200        210        220        230 
LQAKNIRIFY GDSDNDIAAA RELGIRGIRI LRASNSTYKP LPQAGAFGEE VIVNSEY 

« Hide

References

[1]"The Citrobacter rodentium genome sequence reveals convergent evolution with human pathogenic Escherichia coli."
Petty N.K., Bulgin R., Crepin V.F., Cerdeno-Tarraga A.M., Schroeder G.N., Quail M.A., Lennard N., Corton C., Barron A., Clark L., Toribio A.L., Parkhill J., Dougan G., Frankel G., Thomson N.R.
J. Bacteriol. 192:525-538(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ICC168.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
FN543502 Genomic DNA. Translation: CBG90344.1.
RefSeqYP_003367081.1. NC_013716.1.

3D structure databases

ProteinModelPortalD2TRT2.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCBG90344; CBG90344; ROD_36361.
GeneID8711915.
KEGGcro:ROD_36361.
PATRIC32030966. VBICitRod33214_3468.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMHOG000270623.
KOK03788.
OMAPEFWEKM.

Enzyme and pathway databases

BioCycCROD637910:GJIG-3659-MONOMER.

Family and domain databases

Gene3D3.40.50.1000. 1 hit.
InterProIPR005519. Acid_phosphat_B.
IPR023214. HAD-like_dom.
IPR010025. HAD-SF_ppase_IIIB_AphA.
[Graphical view]
PfamPF03767. Acid_phosphat_B. 1 hit.
[Graphical view]
PIRSFPIRSF017818. Acid_Ptase_B. 1 hit.
SUPFAMSSF56784. SSF56784. 1 hit.
TIGRFAMsTIGR01672. AphA. 1 hit.
ProtoNetSearch...

Entry information

Entry nameAPHA_CITRI
AccessionPrimary (citable) accession number: D2TRT2
Entry history
Integrated into UniProtKB/Swiss-Prot: January 25, 2012
Last sequence update: March 2, 2010
Last modified: April 16, 2014
This is version 26 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families