D2TRT2 (APHA_CITRI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Class B acid phosphatase
Short name=CBAP
EC=3.1.3.2

Gene names

Name:	aphA
Ordered Locus Names:	ROD_36361

Organism

Citrobacter rodentium (strain ICC168) (Citrobacter freundii biotype 4280) [Complete proteome] [HAMAP]

Taxonomic identifier

637910 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Citrobacter › Citrobacter rodentium

Protein attributes

Sequence length	237 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Dephosphorylates several organic phosphate monoesters. Also has a phosphotransferase activity catalyzing the transfer of low-energy phosphate groups from organic phosphate monoesters to free hydroxyl groups of various organic compounds By similarity.
Catalytic activity	A phosphate monoester + H2O = an alcohol + phosphate.
Cofactor	Binds 1 magnesium ion per subunit By similarity.
Subunit structure	Homotetramer By similarity.
Subcellular location	Periplasm By similarity.
Sequence similarities	Belongs to the class B bacterial acid phosphatase family.

Ontologies

Keywords
Cellular component	Periplasm
Domain	Signal
Ligand	Magnesium Metal-binding
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Cellular_component	outer membrane-bounded periplasmic space Inferred from electronic annotation. Source: InterPro
Molecular_function	acid phosphatase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 25	25	Potential
	Chain	26 – 237	212	Class B acid phosphatase		PRO_5000566034
Regions
	Region	137 – 138	2	Substrate binding By similarity
Sites
	Active site	69	1	Nucleophile By similarity
	Active site	71	1	Proton donor By similarity
	Metal binding	69	1	Magnesium By similarity
	Metal binding	71	1	Magnesium; via carbonyl oxygen By similarity
	Metal binding	192	1	Magnesium By similarity
	Binding site	177	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	D2TRT2 [UniParc]. Last modified March 2, 2010. Version 1. Checksum: 3BF9EA0904C88479	FASTA	237	26,157
	10 20 30 40 50 60 MRKVSLALSA ACLLFTLNYT ASALASSPSP LNPGTNVAKL AEQAPVHWVS VAQIEKSLTG 70 80 90 100 110 120 RPPMAVGFDI DDTVLFSSPG FWRGKKMYSP DSEEYLKNPA FWEKMNNGWD EFSIPKEVAR 130 140 150 160 170 180 QLIALHLRRG DSIFFVTGRS QTSNERVSKT LADDFLIPAT VMNPVIFAGD KPGQNSKIQW 190 200 210 220 230 LQAKNIRIFY GDSDNDIAAA RELGIRGIRI LRASNSTYKP LPQAGAFGEE VIVNSEY

References

[1]

"The Citrobacter rodentium genome sequence reveals convergent evolution with human pathogenic Escherichia coli." Petty N.K., Bulgin R., Crepin V.F., Cerdeno-Tarraga A.M., Schroeder G.N., Quail M.A., Lennard N., Corton C., Barron A., Clark L., Toribio A.L., Parkhill J., Dougan G., Frankel G., Thomson N.R. J. Bacteriol. 192:525-538(2010) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ICC168.

Cross-references

Sequence databases
EMBL GenBank DDBJ	FN543502 Genomic DNA. Translation: CBG90344.1.
RefSeq	YP_003367081.1. NC_013716.1.
3D structure databases
ProteinModelPortal	D2TRT2.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	CBG90344; CBG90344; ROD_36361.
GeneID	8711915.
KEGG	cro:ROD_36361.
PATRIC	32030966. VBICitRod33214_3468.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HOG000270623.
KO	K03788.
OMA	PEFWEKM.
Enzyme and pathway databases
BioCyc	CROD637910:GJIG-3659-MONOMER.
Family and domain databases
Gene3D	3.40.50.1000. 1 hit.
InterPro	IPR005519. Acid_phosphat_B. IPR023214. HAD-like_dom. IPR010025. HAD-SF_ppase_IIIB_AphA. [Graphical view]
Pfam	PF03767. Acid_phosphat_B. 1 hit. [Graphical view]
PIRSF	PIRSF017818. Acid_Ptase_B. 1 hit.
SUPFAM	SSF56784. HAD-like_dom. 1 hit.
TIGRFAMs	TIGR01672. AphA. 1 hit.
ProtoNet	Search...

Entry information

Entry name

APHA_CITRI

Accession

Primary (citable) accession number: D2TRT2

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 25, 2012
Last sequence update:	March 2, 2010
Last modified:	May 1, 2013
This is version 23 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program