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D2S6E3 (PSA_GEOOG) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 27. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Proteasome subunit alpha

EC=3.4.25.1
Alternative name(s):
20S proteasome alpha subunit
Proteasome core protein PrcA
Gene names
Name:prcA
Ordered Locus Names:Gobs_2683
OrganismGeodermatophilus obscurus (strain ATCC 25078 / DSM 43160 / JCM 3152 / G-20) [Complete proteome] [HAMAP]
Taxonomic identifier526225 [NCBI]
Taxonomic lineageBacteriaActinobacteriaActinobacteridaeActinomycetalesFrankineaeGeodermatophilaceaeGeodermatophilus

Protein attributes

Sequence length287 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Component of the proteasome core, a large protease complex with broad specificity involved in protein degradation By similarity. HAMAP-Rule MF_00289

Catalytic activity

Cleavage of peptide bonds with very broad specificity. HAMAP-Rule MF_00289

Enzyme regulation

The formation of the proteasomal ATPase ARC-20S proteasome complex, likely via the docking of the C-termini of ARC into the intersubunit pockets in the alpha-rings, may trigger opening of the gate for substrate entry. Interconversion between the open-gate and close-gate conformations leads to a dynamic regulation of the 20S proteasome proteolysis activity By similarity. HAMAP-Rule MF_00289

Pathway

Protein degradation; proteasomal Pup-dependent pathway. HAMAP-Rule MF_00289

Subunit structure

The 20S proteasome core is composed of 14 alpha and 14 beta subunits that assemble into four stacked heptameric rings, resulting in a barrel-shaped structure. The two inner rings, each composed of seven catalytic beta subunits, are sandwiched by two outer rings, each composed of seven alpha subunits. The catalytic chamber with the active sites is on the inside of the barrel. Has a gated structure, the ends of the cylinder being occluded by the N-termini of the alpha-subunits. Is capped by the proteasome-associated ATPase, ARC By similarity.

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00289.

Sequence similarities

Belongs to the peptidase T1A family.

Ontologies

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 287287Proteasome subunit alpha HAMAP-Rule MF_00289
PRO_0000397140

Sequences

Sequence LengthMass (Da)Tools
D2S6E3 [UniParc].

Last modified March 2, 2010. Version 1.
Checksum: 8B93882485BE6385

FASTA28730,406
        10         20         30         40         50         60 
MTMPYYASPE QLMRDKSEYA RKGISRGRSV AVVTYADGVL FIAENPSSTL HKVGELYDRI 

        70         80         90        100        110        120 
GFAAVGRYSE FESLRVAGVR LADVRGYSYN RRDVTGRVIA NAYAQTLGEV FTQQMKPFEV 

       130        140        150        160        170        180 
ELCVAEVGET PETDQLYRLT FDGSVVDEPD FVVMGGQAEA VSANLREHFL PGMGLAEALR 

       190        200        210        220        230        240 
VGVQALSAVS PATSAGNGGP ALLTAEQLEV AVLDRRRPKR AFRRIAGAAL RPLLDRQEED 

       250        260        270        280 
GVVAGEEPHT AAHAPSVPQP GAPAGLGDPG APDTGGTAGS GGEAPTT 

« Hide

References

[1]"The complete genome of Geodermatophilus obscurus DSM 43160."
US DOE Joint Genome Institute (JGI-PGF)
Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Kyrpides N., Mavromatis K., Ivanova N., Munk A.C., Brettin T., Detter J.C., Han C., Larimer F., Land M. expand/collapse author list , Hauser L., Markowitz V., Cheng J.-F., Hugenholtz P., Woyke T., Wu D., Jando M., Schneider S., Klenk H.-P., Eisen J.A.
Submitted (JAN-2010) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 25078 / DSM 43160 / JCM 3152 / G-20.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP001867 Genomic DNA. Translation: ADB75317.1.
RefSeqYP_003409688.1. NC_013757.1.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaADB75317; ADB75317; Gobs_2683.
GeneID8754355.
KEGGgob:Gobs_2683.
PATRIC32174492. VBIGeoObs49253_2659.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMHOG000245319.
KOK03432.
OMAGRYNEFE.

Enzyme and pathway databases

BioCycGOBS526225:GI00-2712-MONOMER.
UniPathwayUPA00997.

Family and domain databases

Gene3D3.60.20.10. 1 hit.
HAMAPMF_00289_B. Proteasome_A_B.
InterProIPR029055. Ntn_hydrolases_N.
IPR022296. Proteasome_asu_bac.
IPR023332. Proteasome_suA-type.
IPR001353. Proteasome_sua/b.
[Graphical view]
PfamPF00227. Proteasome. 1 hit.
[Graphical view]
SUPFAMSSF56235. SSF56235. 1 hit.
TIGRFAMsTIGR03691. 20S_bact_alpha. 1 hit.
PROSITEPS51475. PROTEASOME_ALPHA_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePSA_GEOOG
AccessionPrimary (citable) accession number: D2S6E3
Entry history
Integrated into UniProtKB/Swiss-Prot: August 10, 2010
Last sequence update: March 2, 2010
Last modified: June 11, 2014
This is version 27 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Peptidase families

Classification of peptidase families and list of entries

PATHWAY comments

Index of metabolic and biosynthesis pathways