ribBA

Functionⁱ

Catalyzes the conversion of D-ribulose 5-phosphate to formate and 3,4-dihydroxy-2-butanone 4-phosphate.UniRule annotationSAAS annotation

Catalyzes the conversion of GTP to 2,5-diamino-6-ribosylamino-4(3H)-pyrimidinone 5'-phosphate (DARP), formate and pyrophosphate.UniRule annotation

Catalytic activityⁱ

D-ribulose 5-phosphate = formate + L-3,4-dihydroxybutan-2-one 4-phosphate.UniRule annotation

GTP + 3 H₂O = formate + 2,5-diamino-6-hydroxy-4-(5-phospho-D-ribosylamino)pyrimidine + diphosphate.UniRule annotationSAAS annotation

Cofactorⁱ

Mg²⁺UniRule annotation, Mn²⁺UniRule annotationNote: Binds 2 divalent metal cations per subunit. Magnesium or manganese.UniRule annotation

Pathwayⁱ: riboflavin biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes 2-hydroxy-3-oxobutyl phosphate from D-ribulose 5-phosphate.UniRule annotation
Proteins known to be involved in this subpathway in this organism are:

Riboflavin biosynthesis protein RibBA (ribBA)

This subpathway is part of the pathway riboflavin biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 2-hydroxy-3-oxobutyl phosphate from D-ribulose 5-phosphate, the pathway riboflavin biosynthesis and in Cofactor biosynthesis.

Pathwayⁱ: riboflavin biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes 5-amino-6-(D-ribitylamino)uracil from GTP.SAAS annotation
Proteins known to be involved in the 4 steps of the subpathway in this organism are:

Riboflavin biosynthesis protein RibBA (ribBA)
Riboflavin biosynthesis protein RibD (Acfer_0353)
Riboflavin biosynthesis protein RibD (Acfer_0353)
no protein annotated in this organism

This subpathway is part of the pathway riboflavin biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 5-amino-6-(D-ribitylamino)uracil from GTP, the pathway riboflavin biosynthesis and in Cofactor biosynthesis.

Sites

Feature key	Position(s)	DescriptionActions	Length
Metal bindingⁱ	32	Magnesium or manganese 1UniRule annotation	1
Metal bindingⁱ	32	Magnesium or manganese 2UniRule annotation	1
Binding siteⁱ	36	D-ribulose 5-phosphateUniRule annotation	1
Siteⁱ	129	Essential for DHBP synthase activityUniRule annotation	1
Metal bindingⁱ	146	Magnesium or manganese 2UniRule annotation	1
Binding siteⁱ	167	D-ribulose 5-phosphateUniRule annotation	1
Siteⁱ	167	Essential for DHBP synthase activityUniRule annotation	1
Metal bindingⁱ	260	Zinc; catalyticUniRule annotation	1
Metal bindingⁱ	271	Zinc; catalyticUniRule annotation	1
Metal bindingⁱ	273	Zinc; catalyticUniRule annotation	1
Binding siteⁱ	276	GTPUniRule annotation	1
Binding siteⁱ	320	GTPUniRule annotation	1
Active siteⁱ	332	Proton acceptor; for GTP cyclohydrolase activityUniRule annotation	1
Active siteⁱ	334	Nucleophile; for GTP cyclohydrolase activityUniRule annotation	1
Binding siteⁱ	355	GTPUniRule annotation	1
Binding siteⁱ	360	GTPUniRule annotation	1

Regions

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Nucleotide bindingⁱ	255 – 259	GTPUniRule annotation		5
Nucleotide bindingⁱ	298 – 300	GTPUniRule annotation		3

GO - Molecular functionⁱ

3,4-dihydroxy-2-butanone-4-phosphate synthase activity Source: UniProtKB-UniRule
GTP binding Source: UniProtKB-KW
GTP cyclohydrolase II activity Source: UniProtKB-UniRule
magnesium ion binding Source: UniProtKB-UniRule
manganese ion binding Source: UniProtKB-UniRule
zinc ion binding Source: UniProtKB-UniRule

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

riboflavin biosynthetic process Source: UniProtKB-UniRule

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	HydrolaseUniRule annotationSAAS annotation, LyaseUniRule annotation, Multifunctional enzymeUniRule annotation
Biological process	Riboflavin biosynthesisUniRule annotationSAAS annotation
Ligand	GTP-bindingUniRule annotationSAAS annotation, MagnesiumUniRule annotation, ManganeseUniRule annotation, Metal-bindingUniRule annotationSAAS annotation, Nucleotide-binding, ZincUniRule annotationSAAS annotation

Enzyme and pathway databases

BioCycⁱ	AFER591001:G1GH7-375-MONOMER
UniPathwayⁱ	UPA00275; UER00399 UPA00275; UER00400

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Riboflavin biosynthesis protein RibBAUniRule annotation Including the following 2 domains: GTP cyclohydrolase-2UniRule annotation (EC:3.5.4.25UniRule annotation) Alternative name(s): GTP cyclohydrolase IIUniRule annotation 3,4-dihydroxy-2-butanone 4-phosphate synthaseUniRule annotation (EC:4.1.99.12UniRule annotation) Short name: DHBP synthaseUniRule annotation
Gene namesⁱ	Name:ribBAUniRule annotation Ordered Locus Names:Acfer_0355Imported
Organismⁱ	Acidaminococcus fermentans (strain ATCC 25085 / DSM 20731 / VR4)Imported
Taxonomic identifierⁱ	591001 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Bacteria › Terrabacteria group › Firmicutes › Negativicutes › Acidaminococcales › Acidaminococcaceae › Acidaminococcus › Acidaminococcus fermentans
Proteomesⁱ	UP000001902 Componentⁱ: Chromosome

Interactionⁱ

Protein-protein interaction databases

STRINGⁱ	591001.Acfer_0355

STRINGⁱ

591001.Acfer_0355

Family & Domainsⁱ

Domains and Repeats

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Domainⁱ	211 – 376	GTP_cyclohydro2InterPro annotationAdd BLAST		166

Region

Feature key	Position(s)	DescriptionActions	Length
Regionⁱ	1 – 204	DHBP synthaseUniRule annotationAdd BLAST	204
Regionⁱ	31 – 32	D-ribulose 5-phosphate bindingUniRule annotation	2
Regionⁱ	143 – 147	D-ribulose 5-phosphate bindingUniRule annotation	5
Regionⁱ	205 – 408	GTP cyclohydrolase IIUniRule annotationAdd BLAST	204

Sequence similaritiesⁱ

In the C-terminal section; belongs to the GTP cyclohydrolase II family.UniRule annotationSAAS annotation

In the N-terminal section; belongs to the DHBP synthase family.UniRule annotationSAAS annotation

Phylogenomic databases

eggNOGⁱ	ENOG4105C66 Bacteria COG0108 LUCA COG0807 LUCA
HOGENOMⁱ	HOG000115440
KEGG Orthology (KO) More...KOⁱ	K14652
OMAⁱ	GCTTGIS
Database of Orthologous Groups More...OrthoDBⁱ	POG091H008U

Family and domain databases

Conserved Domains Database More...CDDⁱ	cd00641 GTP_cyclohydro2, 1 hit
Gene3Dⁱ	3.40.50.10990, 1 hit
HAMAPⁱ	MF_00179 RibA, 1 hit MF_00180 RibB, 1 hit MF_01283 RibBA, 1 hit
InterProⁱ	View protein in InterPro IPR017945 DHBP_synth_RibB-like_a/b_dom IPR000422 DHBP_synthase_RibB IPR032677 GTP_cyclohydro_II IPR000926 RibA IPR036144 RibA-like_sf IPR016299 Riboflavin_synth_RibBA
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF00926 DHBP_synthase, 1 hit PF00925 GTP_cyclohydro2, 1 hit
SUPFAMⁱ	SSF142695 SSF142695, 1 hit SSF55821 SSF55821, 1 hit
TIGRFAMsⁱ	TIGR00505 ribA, 1 hit TIGR00506 ribB, 1 hit

Sequenceⁱ

Sequence statusⁱ: Complete.

D2RNY5-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MDKKFQFAPI EQAIEDIRAG KMVLVTDDPD RENEGDLIMS AEYVTPDDIN 
        60         70         80         90        100
FMATHAKGLI CMPCDGAILD RLQMEPMVAN NTDNHETAFT VSIDHKDTTT 
       110        120        130        140        150
GISAVERAYT IKKCTDESAK PEDFRRPGHV FPLRSREGGV LRRTGHTETT 
       160        170        180        190        200
TDLCRLAGLK PVGICCEIMS ADGTMARTPE LIEFARKFNL TFITVADLIA 
       210        220        230        240        250
YRKKNEKMVH RIANVALPSK YGTFRAIGYE NDLDDKCHVA IIKGDVAGKK 
       260        270        280        290        300
DVLVRVHSEC LTGDALGSLR CDCGDQLATS LKMIEKEGCG VVLYMQQEGR 
       310        320        330        340        350
GIGLANKLRA YELQDQGLDT VDANVKLGFK PDMRDYGIGA QILADLGLTS 
       360        370        380        390        400
IRLMTNNPAK RAGLSGYGIT ITETVPIIMK DNCYNHRYMV TKQVRMGHEL 

HEDVEDAK

Length:408

Mass (Da):45,199

Last modified:March 2, 2010 - v1

Checksum:ⁱE9C03148F8FE9109

GO

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	CP001859 Genomic DNA Translation: ADB46761.1
NCBI Reference Sequences More...RefSeqⁱ	WP_012937751.1, NC_013740.1

Genome annotation databases

EnsemblBacteriaⁱ	ADB46761; ADB46761; Acfer_0355
KEGGⁱ	afn:Acfer_0355

Protein	Similar proteins		Species	Score	Length
D2RNY5	Riboflavin biosynthesis protein RibBA		ACIFE		408
UPI00094E1FDC		Acidaminococcus massiliensis		408
UPI0008D9CABD		Acidaminococcus timonensis		407
UPI00089CF505		ACIFE		408

Protein	Similar proteins		Species	Score	Length
D2RNY5	Riboflavin biosynthesis protein RibBA		BACSU		398
Riboflavin biosynthesis protein RibBA		Bacillus subtilis subsp. subtilis		398
Riboflavin biosynthesis protein RibBA		BACIU		398
Riboflavin biosynthesis protein RibBA		Bacillus subtilis BEST7613		398
UPI000B8BC13D		Bacillus sp. LYLB4		400
+3845

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	CP001859 Genomic DNA Translation: ADB46761.1
NCBI Reference Sequences More...RefSeqⁱ	WP_012937751.1, NC_013740.1

3D structure databases

ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

STRINGⁱ	591001.Acfer_0355

STRINGⁱ

591001.Acfer_0355

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

EnsemblBacteriaⁱ	ADB46761; ADB46761; Acfer_0355
KEGGⁱ	afn:Acfer_0355

Phylogenomic databases

eggNOGⁱ	ENOG4105C66 Bacteria COG0108 LUCA COG0807 LUCA
HOGENOMⁱ	HOG000115440
KEGG Orthology (KO) More...KOⁱ	K14652
OMAⁱ	GCTTGIS
Database of Orthologous Groups More...OrthoDBⁱ	POG091H008U

Enzyme and pathway databases

UniPathwayⁱ	UPA00275; UER00399 UPA00275; UER00400
BioCycⁱ	AFER591001:G1GH7-375-MONOMER

Family and domain databases

Conserved Domains Database More...CDDⁱ	cd00641 GTP_cyclohydro2, 1 hit
Gene3Dⁱ	3.40.50.10990, 1 hit
HAMAPⁱ	MF_00179 RibA, 1 hit MF_00180 RibB, 1 hit MF_01283 RibBA, 1 hit
InterProⁱ	View protein in InterPro IPR017945 DHBP_synth_RibB-like_a/b_dom IPR000422 DHBP_synthase_RibB IPR032677 GTP_cyclohydro_II IPR000926 RibA IPR036144 RibA-like_sf IPR016299 Riboflavin_synth_RibBA
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF00926 DHBP_synthase, 1 hit PF00925 GTP_cyclohydro2, 1 hit
SUPFAMⁱ	SSF142695 SSF142695, 1 hit SSF55821 SSF55821, 1 hit
TIGRFAMsⁱ	TIGR00505 ribA, 1 hit TIGR00506 ribB, 1 hit
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	D2RNY5_ACIFV
Accessionⁱ	D2RNY5Primary (citable) accession number: D2RNY5
Entry historyⁱ	Integrated into UniProtKB/TrEMBL:	March 2, 2010
	Last sequence update:	March 2, 2010
	Last modified:	May 23, 2018
	This is version 68 of the entry and version 1 of the sequence. See complete history.
Entry statusⁱ	Unreviewed (UniProtKB/TrEMBL)

Miscellaneousⁱ

Keywords - Technical termⁱ

Complete proteome, Reference proteomeImported

UniProtKB

UniParc

Help

UniRef

Proteomes

Annotation systems

Supporting data

UniProtKB - D2RNY5 (D2RNY5_ACIFV)

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Riboflavin biosynthesis protein RibBA

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">'Function'</a> section describes the metabolic pathway(s) associated with a protein.<p><a href='/help/pathway' target='_top'>More...</a></p>Pathwayi: riboflavin biosynthesis

<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">'Function'</a> section describes the metabolic pathway(s) associated with a protein.<p><a href='/help/pathway' target='_top'>More...</a></p>Pathwayi: riboflavin biosynthesis

Sites

Regions

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Biological processi

Enzyme and pathway databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

Protein-protein interaction databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

Region

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Phylogenomic databases

Family and domain databases

Sequence databases

Genome annotation databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

Protocols and materials databases

Genome annotation databases

Phylogenomic databases

Enzyme and pathway databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

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Functionⁱ

Pathwayⁱ: riboflavin biosynthesis

Pathwayⁱ: riboflavin biosynthesis

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Interactionⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ