ribBA

Functionⁱ

Catalyzes the conversion of D-ribulose 5-phosphate to formate and 3,4-dihydroxy-2-butanone 4-phosphate.UniRule annotationSAAS annotation

Catalyzes the conversion of GTP to 2,5-diamino-6-ribosylamino-4(3H)-pyrimidinone 5'-phosphate (DARP), formate and pyrophosphate.UniRule annotation

Catalytic activityⁱ

D-ribulose 5-phosphate = formate + L-3,4-dihydroxybutan-2-one 4-phosphate.UniRule annotation

GTP + 3 H₂O = formate + 2,5-diamino-6-hydroxy-4-(5-phospho-D-ribosylamino)pyrimidine + diphosphate.UniRule annotationSAAS annotation

Cofactorⁱ

Mg²⁺UniRule annotation, Mn²⁺UniRule annotationNote: Binds 2 divalent metal cations per subunit. Magnesium or manganese.UniRule annotation

Pathwayⁱ: riboflavin biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes 2-hydroxy-3-oxobutyl phosphate from D-ribulose 5-phosphate.UniRule annotation
Proteins known to be involved in this subpathway in this organism are:

Riboflavin biosynthesis protein RibBA (ribBA)

This subpathway is part of the pathway riboflavin biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 2-hydroxy-3-oxobutyl phosphate from D-ribulose 5-phosphate, the pathway riboflavin biosynthesis and in Cofactor biosynthesis.

Pathwayⁱ: riboflavin biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes 5-amino-6-(D-ribitylamino)uracil from GTP.SAAS annotation
Proteins known to be involved in the 4 steps of the subpathway in this organism are:

Riboflavin biosynthesis protein RibBA (ribBA)
Riboflavin biosynthesis protein RibD (Acfer_0353)
Riboflavin biosynthesis protein RibD (Acfer_0353)
no protein annotated in this organism

This subpathway is part of the pathway riboflavin biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 5-amino-6-(D-ribitylamino)uracil from GTP, the pathway riboflavin biosynthesis and in Cofactor biosynthesis.

Sites

Feature key	Position(s)	DescriptionActions	Length
Metal bindingⁱ	32	Magnesium or manganese 1UniRule annotation	1
Metal bindingⁱ	32	Magnesium or manganese 2UniRule annotation	1
Binding siteⁱ	36	D-ribulose 5-phosphateUniRule annotation	1
Siteⁱ	129	Essential for DHBP synthase activityUniRule annotation	1
Metal bindingⁱ	146	Magnesium or manganese 2UniRule annotation	1
Binding siteⁱ	167	D-ribulose 5-phosphateUniRule annotation	1
Siteⁱ	167	Essential for DHBP synthase activityUniRule annotation	1
Metal bindingⁱ	260	Zinc; catalyticUniRule annotation	1
Metal bindingⁱ	271	Zinc; catalyticUniRule annotation	1
Metal bindingⁱ	273	Zinc; catalyticUniRule annotation	1
Binding siteⁱ	276	GTPUniRule annotation	1
Binding siteⁱ	320	GTPUniRule annotation	1
Active siteⁱ	332	Proton acceptor; for GTP cyclohydrolase activityUniRule annotation	1
Active siteⁱ	334	Nucleophile; for GTP cyclohydrolase activityUniRule annotation	1
Binding siteⁱ	355	GTPUniRule annotation	1
Binding siteⁱ	360	GTPUniRule annotation	1

Regions

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Nucleotide bindingⁱ	255 – 259	GTPUniRule annotation		5
Nucleotide bindingⁱ	298 – 300	GTPUniRule annotation		3

GO - Molecular functionⁱ

3,4-dihydroxy-2-butanone-4-phosphate synthase activity Source: UniProtKB-UniRule
GTP binding Source: UniProtKB-KW
GTP cyclohydrolase II activity Source: UniProtKB-UniRule
magnesium ion binding Source: UniProtKB-UniRule
manganese ion binding Source: UniProtKB-UniRule
zinc ion binding Source: UniProtKB-UniRule

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

riboflavin biosynthetic process Source: UniProtKB-UniRule

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	HydrolaseUniRule annotation, LyaseUniRule annotationSAAS annotation, Multifunctional enzymeUniRule annotation
Biological process	Riboflavin biosynthesisUniRule annotationSAAS annotation
Ligand	GTP-bindingUniRule annotationSAAS annotation, MagnesiumUniRule annotation, ManganeseUniRule annotation, Metal-bindingUniRule annotationSAAS annotation, Nucleotide-binding, ZincUniRule annotationSAAS annotation

Enzyme and pathway databases

BioCycⁱ	AFER591001:G1GH7-375-MONOMER.
UniPathwayⁱ	UPA00275; UER00399. UPA00275; UER00400.

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Riboflavin biosynthesis protein RibBAUniRule annotation Including the following 2 domains: 3,4-dihydroxy-2-butanone 4-phosphate synthaseUniRule annotation (EC:4.1.99.12UniRule annotation) Short name: DHBP synthaseUniRule annotation GTP cyclohydrolase-2UniRule annotation (EC:3.5.4.25UniRule annotation) Alternative name(s): GTP cyclohydrolase IIUniRule annotation
Gene namesⁱ	Name:ribBAUniRule annotation Ordered Locus Names:Acfer_0355Imported
Organismⁱ	Acidaminococcus fermentans (strain ATCC 25085 / DSM 20731 / VR4)Imported
Taxonomic identifierⁱ	591001 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Bacteria › Terrabacteria group › Firmicutes › Negativicutes › Acidaminococcales › Acidaminococcaceae › Acidaminococcus › Acidaminococcus fermentans
Proteomesⁱ	UP000001902 Componentⁱ: Chromosome

Interactionⁱ

Protein-protein interaction databases

STRINGⁱ	591001.Acfer_0355.

STRINGⁱ

591001.Acfer_0355.

Family & Domainsⁱ

Domains and Repeats

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Domainⁱ	211 – 376	GTP_cyclohydro2InterPro annotationAdd BLAST		166

Region

Feature key	Position(s)	DescriptionActions	Length
Regionⁱ	1 – 204	DHBP synthaseUniRule annotationAdd BLAST	204
Regionⁱ	31 – 32	D-ribulose 5-phosphate bindingUniRule annotation	2
Regionⁱ	143 – 147	D-ribulose 5-phosphate bindingUniRule annotation	5
Regionⁱ	205 – 408	GTP cyclohydrolase IIUniRule annotationAdd BLAST	204

Sequence similaritiesⁱ

In the C-terminal section; belongs to the GTP cyclohydrolase II family.UniRule annotationSAAS annotation

In the N-terminal section; belongs to the DHBP synthase family.UniRule annotationSAAS annotation

Phylogenomic databases

eggNOGⁱ	ENOG4105C66. Bacteria. COG0108. LUCA. COG0807. LUCA.
HOGENOMⁱ	HOG000115440.
KEGG Orthology (KO) More...KOⁱ	K14652.
OMAⁱ	GCTTGIS.
Database of Orthologous Groups More...OrthoDBⁱ	POG091H008U.

Family and domain databases

Conserved Domains Database More...CDDⁱ	cd00641. GTP_cyclohydro2. 1 hit.
Gene3Dⁱ	3.40.50.10990. 1 hit.
HAMAPⁱ	MF_00179. RibA. 1 hit. MF_00180. RibB. 1 hit. MF_01283. RibBA. 1 hit.
InterProⁱ	View protein in InterPro IPR017945. DHBP_synth_RibB-like_a/b_dom. IPR000422. DHBP_synthase_RibB. IPR032677. GTP_cyclohydro_II. IPR000926. RibA. IPR036144. RibA-like_sf. IPR016299. Riboflavin_synth_RibBA.
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF00926. DHBP_synthase. 1 hit. PF00925. GTP_cyclohydro2. 1 hit.
SUPFAMⁱ	SSF142695. SSF142695. 1 hit. SSF55821. SSF55821. 1 hit.
TIGRFAMsⁱ	TIGR00505. ribA. 1 hit. TIGR00506. ribB. 1 hit.

Sequenceⁱ

Sequence statusⁱ: Complete.

D2RNY5-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MDKKFQFAPI EQAIEDIRAG KMVLVTDDPD RENEGDLIMS AEYVTPDDIN 
        60         70         80         90        100
FMATHAKGLI CMPCDGAILD RLQMEPMVAN NTDNHETAFT VSIDHKDTTT 
       110        120        130        140        150
GISAVERAYT IKKCTDESAK PEDFRRPGHV FPLRSREGGV LRRTGHTETT 
       160        170        180        190        200
TDLCRLAGLK PVGICCEIMS ADGTMARTPE LIEFARKFNL TFITVADLIA 
       210        220        230        240        250
YRKKNEKMVH RIANVALPSK YGTFRAIGYE NDLDDKCHVA IIKGDVAGKK 
       260        270        280        290        300
DVLVRVHSEC LTGDALGSLR CDCGDQLATS LKMIEKEGCG VVLYMQQEGR 
       310        320        330        340        350
GIGLANKLRA YELQDQGLDT VDANVKLGFK PDMRDYGIGA QILADLGLTS 
       360        370        380        390        400
IRLMTNNPAK RAGLSGYGIT ITETVPIIMK DNCYNHRYMV TKQVRMGHEL 

HEDVEDAK

Length:408

Mass (Da):45,199

Last modified:March 2, 2010 - v1

Checksum:ⁱE9C03148F8FE9109

GO

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	CP001859 Genomic DNA. Translation: ADB46761.1.
NCBI Reference Sequences More...RefSeqⁱ	WP_012937751.1. NC_013740.1.

Genome annotation databases

EnsemblBacteriaⁱ	ADB46761; ADB46761; Acfer_0355.
KEGGⁱ	afn:Acfer_0355.

Protein	Similar proteins	Organisms	Length	Cluster ID	Cluster name	Size
D2RNY5	A0A1I5I1C1 UPI00089CF505 UPI00094E1FDC UPI0008D9CABD	Acidaminococcus fermentans Acidaminococcus massiliensis Acidaminococcus timonensis	408	UniRef90_D2RNY5	Cluster: Riboflavin biosynthesis protein RibBA	5

Protein	Similar proteins	Organisms	Length	Cluster ID	Cluster name	Size
D2RNY5	UPI00094E1FDC A0A1I5I1C1 UPI00089CF505 UPI0008D9CABD A0A2D0NDW4 UPI000B83CE19 A0A1I7BC21 A0A1I0XN71 A0A1I2WVQ9 A0A1I5JPQ1 +54	Acidaminococcus massiliensis Acidaminococcus fermentans Acidaminococcus timonensis Lewinella nigricans DSM 23189 = NBRC 102662 Algoriphagus locisalis Algoriphagus aquimarinus Algoriphagus hitonicola Algoriphagus ornithinivorans Algoriphagus sp. Algoriphagus resistens And more	408	UniRef50_D2RNY5	Cluster: Riboflavin biosynthesis protein RibBA	65

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	CP001859 Genomic DNA. Translation: ADB46761.1.
NCBI Reference Sequences More...RefSeqⁱ	WP_012937751.1. NC_013740.1.

3D structure databases

ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

STRINGⁱ	591001.Acfer_0355.

STRINGⁱ

591001.Acfer_0355.

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

EnsemblBacteriaⁱ	ADB46761; ADB46761; Acfer_0355.
KEGGⁱ	afn:Acfer_0355.

Phylogenomic databases

eggNOGⁱ	ENOG4105C66. Bacteria. COG0108. LUCA. COG0807. LUCA.
HOGENOMⁱ	HOG000115440.
KEGG Orthology (KO) More...KOⁱ	K14652.
OMAⁱ	GCTTGIS.
Database of Orthologous Groups More...OrthoDBⁱ	POG091H008U.

Enzyme and pathway databases

UniPathwayⁱ	UPA00275; UER00399. UPA00275; UER00400.
BioCycⁱ	AFER591001:G1GH7-375-MONOMER.

Family and domain databases

Conserved Domains Database More...CDDⁱ	cd00641. GTP_cyclohydro2. 1 hit.
Gene3Dⁱ	3.40.50.10990. 1 hit.
HAMAPⁱ	MF_00179. RibA. 1 hit. MF_00180. RibB. 1 hit. MF_01283. RibBA. 1 hit.
InterProⁱ	View protein in InterPro IPR017945. DHBP_synth_RibB-like_a/b_dom. IPR000422. DHBP_synthase_RibB. IPR032677. GTP_cyclohydro_II. IPR000926. RibA. IPR036144. RibA-like_sf. IPR016299. Riboflavin_synth_RibBA.
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF00926. DHBP_synthase. 1 hit. PF00925. GTP_cyclohydro2. 1 hit.
SUPFAMⁱ	SSF142695. SSF142695. 1 hit. SSF55821. SSF55821. 1 hit.
TIGRFAMsⁱ	TIGR00505. ribA. 1 hit. TIGR00506. ribB. 1 hit.
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	D2RNY5_ACIFV
Accessionⁱ	D2RNY5Primary (citable) accession number: D2RNY5
Entry historyⁱ	Integrated into UniProtKB/TrEMBL:	March 2, 2010
	Last sequence update:	March 2, 2010
	Last modified:	March 28, 2018
	This is version 66 of the entry and version 1 of the sequence. See complete history.
Entry statusⁱ	Unreviewed (UniProtKB/TrEMBL)

Miscellaneousⁱ

Keywords - Technical termⁱ

Complete proteome, Reference proteomeImported

UniProtKB

UniParc

Help

UniRef

Proteomes

Annotation systems

Supporting data

UniProtKB - D2RNY5 (D2RNY5_ACIFV)

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Riboflavin biosynthesis protein RibBA

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">‘Function’</a> section describes the metabolic pathway(s) associated with a protein.<p><a href='/help/pathway' target='_top'>More...</a></p>Pathwayi: riboflavin biosynthesis

<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">‘Function’</a> section describes the metabolic pathway(s) associated with a protein.<p><a href='/help/pathway' target='_top'>More...</a></p>Pathwayi: riboflavin biosynthesis

Sites

Regions

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Biological processi

Enzyme and pathway databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

Protein-protein interaction databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

Region

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Phylogenomic databases

Family and domain databases

Sequence databases

Genome annotation databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

Protocols and materials databases

Genome annotation databases

Phylogenomic databases

Enzyme and pathway databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

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Functionⁱ

Pathwayⁱ: riboflavin biosynthesis

Pathwayⁱ: riboflavin biosynthesis

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Interactionⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ