Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

D2RKY0

- D2RKY0_ACIFV

UniProt

D2RKY0 - D2RKY0_ACIFV

Protein

Proline--tRNA ligase

Gene

proS

Organism
Acidaminococcus fermentans (strain ATCC 25085 / DSM 20731 / VR4)
Status
Unreviewed - Annotation score: 2 out of 5- Protein inferred from homologyi
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 35 (01 Oct 2014)
      Sequence version 1 (02 Mar 2010)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction: proline is first activated by ATP to form Pro-AMP and then transferred to the acceptor end of tRNA(Pro). As ProRS can inadvertently accommodate and process non-cognate amino acids such as alanine and cysteine, to avoid such errors it has two additional distinct editing activities against alanine. One activity is designated as 'pretransfer' editing and involves the tRNA(Pro)-independent hydrolysis of activated Ala-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Ala-tRNA(Pro). The misacylated Cys-tRNA(Pro) is not edited by ProRS.UniRule annotationSAAS annotation

    Catalytic activityi

    ATP + L-proline + tRNA(Pro) = AMP + diphosphate + L-prolyl-tRNA(Pro).UniRule annotationSAAS annotation

    GO - Molecular functioni

    1. aminoacyl-tRNA editing activity Source: InterPro
    2. ATP binding Source: UniProtKB-KW
    3. proline-tRNA ligase activity Source: UniProtKB-EC

    GO - Biological processi

    1. prolyl-tRNA aminoacylation Source: InterPro

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetaseUniRule annotationSAAS annotationImported, Ligase

    Keywords - Biological processi

    Protein biosynthesisUniRule annotationSAAS annotation

    Keywords - Ligandi

    ATP-bindingUniRule annotationSAAS annotation, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciAFER591001:GHUL-1427-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Proline--tRNA ligaseUniRule annotation (EC:6.1.1.15UniRule annotation)
    Alternative name(s):
    Prolyl-tRNA synthetaseUniRule annotation
    Gene namesi
    Name:proSUniRule annotation
    Ordered Locus Names:Acfer_1372Imported
    OrganismiAcidaminococcus fermentans (strain ATCC 25085 / DSM 20731 / VR4)Imported
    Taxonomic identifieri591001 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesNegativicutesSelenomonadalesAcidaminococcaceaeAcidaminococcus
    ProteomesiUP000001902: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotationSAAS annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    CytoplasmUniRule annotationSAAS annotation

    Interactioni

    Subunit structurei

    Homodimer.UniRule annotationSAAS annotation

    Family & Domainsi

    Domaini

    Consists of three domains: the N-terminal catalytic domain, the editing domain and the C-terminal anticodon-binding domain.UniRule annotation

    Sequence similaritiesi

    Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 1 subfamily.UniRule annotation

    Phylogenomic databases

    HOGENOMiHOG000076893.
    KOiK01881.
    OMAiMHQAYCN.

    Family and domain databases

    Gene3Di3.40.50.800. 1 hit.
    3.90.960.10. 1 hit.
    HAMAPiMF_01569. Pro_tRNA_synth_type1.
    InterProiIPR002314. aa-tRNA-synt_IIb_cons-dom.
    IPR006195. aa-tRNA-synth_II.
    IPR004154. Anticodon-bd.
    IPR002316. Pro-tRNA-ligase_IIa.
    IPR004500. Pro-tRNA-synth_IIa_bac-type.
    IPR023717. Pro-tRNA-Synthase_IIa_type1.
    IPR007214. YbaK/aa-tRNA-synth-assoc-dom.
    [Graphical view]
    PANTHERiPTHR11451:SF3. PTHR11451:SF3. 1 hit.
    PfamiPF03129. HGTP_anticodon. 1 hit.
    PF00587. tRNA-synt_2b. 1 hit.
    PF04073. tRNA_edit. 1 hit.
    [Graphical view]
    PRINTSiPR01046. TRNASYNTHPRO.
    SUPFAMiSSF52954. SSF52954. 1 hit.
    SSF55826. SSF55826. 1 hit.
    TIGRFAMsiTIGR00409. proS_fam_II. 1 hit.
    PROSITEiPS50862. AA_TRNA_LIGASE_II. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    D2RKY0-1 [UniParc]FASTAAdd to Basket

    « Hide

    MRVSQLYAPT LREVPAEAVI ESHKLMLRAG YIRKVAGGLY SYLPLAWRSL    50
    RKIEEIIREE MVPTGAQEIM MPIVQPSEIW QESGRWPAYG AEMFKLKDRH 100
    GRDFCLGPTH EELVTTLVRD DLRSYRQLPV TLYQIQSKFR DEKRPRFGLM 150
    RSREFIMKDA YSFDKDAEGL DKSYQDEYDA YSRIFTRCGL DYRPVEADSG 200
    AIGGKGSHEF MALADSGEAG IVYCDTCEYA ADVEKAECAA IEAPAEEPKE 250
    LEKKATPDCS TIEAVCQYLG SPIEKSVKAV AFVTDEGKLV LCFVRGDKEV 300
    NDTKVVNAVG CNEVDMAPDD LIREAGTVPG FMGPIGLNKD KAIILIDHTV 350
    MHMHNVCCGA NEKDVHYVNA EPSRDFVYTQ VADISTAVAG DKCPHCDGHL 400
    KEARGIEVGQ VFKLNTKYSE ALHATYLDVD GKEHPLVMGC YGIGVGRTLA 450
    AAIEQHHDKD GIIMPRNIAP YQVMVLAMNV KDEESWQKAV EIHDALNQAG 500
    IDTLLDDRDE RAGVKFKDAD LIGCPLRIVI GPKTMKRNAM ETKLRCNGEM 550
    ADVSFEGDWI QAIRDILDKA L 571
    Length:571
    Mass (Da):63,702
    Last modified:March 2, 2010 - v1
    Checksum:i7BE96F8A2F657E42
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP001859 Genomic DNA. Translation: ADB47732.1.
    RefSeqiWP_012938717.1. NC_013740.1.
    YP_003399047.1. NC_013740.1.

    Genome annotation databases

    EnsemblBacteriaiADB47732; ADB47732; Acfer_1372.
    GeneIDi8737857.
    KEGGiafn:Acfer_1372.
    PATRICi31909273. VBIAciFer109666_1348.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP001859 Genomic DNA. Translation: ADB47732.1 .
    RefSeqi WP_012938717.1. NC_013740.1.
    YP_003399047.1. NC_013740.1.

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ADB47732 ; ADB47732 ; Acfer_1372 .
    GeneIDi 8737857.
    KEGGi afn:Acfer_1372.
    PATRICi 31909273. VBIAciFer109666_1348.

    Phylogenomic databases

    HOGENOMi HOG000076893.
    KOi K01881.
    OMAi MHQAYCN.

    Enzyme and pathway databases

    BioCyci AFER591001:GHUL-1427-MONOMER.

    Family and domain databases

    Gene3Di 3.40.50.800. 1 hit.
    3.90.960.10. 1 hit.
    HAMAPi MF_01569. Pro_tRNA_synth_type1.
    InterProi IPR002314. aa-tRNA-synt_IIb_cons-dom.
    IPR006195. aa-tRNA-synth_II.
    IPR004154. Anticodon-bd.
    IPR002316. Pro-tRNA-ligase_IIa.
    IPR004500. Pro-tRNA-synth_IIa_bac-type.
    IPR023717. Pro-tRNA-Synthase_IIa_type1.
    IPR007214. YbaK/aa-tRNA-synth-assoc-dom.
    [Graphical view ]
    PANTHERi PTHR11451:SF3. PTHR11451:SF3. 1 hit.
    Pfami PF03129. HGTP_anticodon. 1 hit.
    PF00587. tRNA-synt_2b. 1 hit.
    PF04073. tRNA_edit. 1 hit.
    [Graphical view ]
    PRINTSi PR01046. TRNASYNTHPRO.
    SUPFAMi SSF52954. SSF52954. 1 hit.
    SSF55826. SSF55826. 1 hit.
    TIGRFAMsi TIGR00409. proS_fam_II. 1 hit.
    PROSITEi PS50862. AA_TRNA_LIGASE_II. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 25085 / DSM 20731 / VR4Imported.

    Entry informationi

    Entry nameiD2RKY0_ACIFV
    AccessioniPrimary (citable) accession number: D2RKY0
    Entry historyi
    Integrated into UniProtKB/TrEMBL: March 2, 2010
    Last sequence update: March 2, 2010
    Last modified: October 1, 2014
    This is version 35 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiUnreviewed (UniProtKB/TrEMBL)

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteomeImported

    External Data

    Dasty 3