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Protein

Proline--tRNA ligase

Gene

proS

Organism
Acidaminococcus fermentans (strain ATCC 25085 / DSM 20731 / VR4)
Status
Unreviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction: proline is first activated by ATP to form Pro-AMP and then transferred to the acceptor end of tRNA(Pro). As ProRS can inadvertently accommodate and process non-cognate amino acids such as alanine and cysteine, to avoid such errors it has two additional distinct editing activities against alanine. One activity is designated as 'pretransfer' editing and involves the tRNA(Pro)-independent hydrolysis of activated Ala-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Ala-tRNA(Pro). The misacylated Cys-tRNA(Pro) is not edited by ProRS.UniRule annotationSAAS annotation

Catalytic activityi

ATP + L-proline + tRNA(Pro) = AMP + diphosphate + L-prolyl-tRNA(Pro).UniRule annotationSAAS annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetaseUniRule annotationSAAS annotation, Ligase

Keywords - Biological processi

Protein biosynthesisUniRule annotationSAAS annotation

Keywords - Ligandi

ATP-bindingUniRule annotationSAAS annotation, Nucleotide-binding

Enzyme and pathway databases

BioCyciAFER591001:GHUL-1427-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Proline--tRNA ligaseUniRule annotationSAAS annotation (EC:6.1.1.15UniRule annotationSAAS annotation)
Alternative name(s):
Prolyl-tRNA synthetaseUniRule annotation
Gene namesi
Name:proSUniRule annotation
Ordered Locus Names:Acfer_1372Imported
OrganismiAcidaminococcus fermentans (strain ATCC 25085 / DSM 20731 / VR4)Imported
Taxonomic identifieri591001 [NCBI]
Taxonomic lineageiBacteriaFirmicutesNegativicutesSelenomonadalesAcidaminococcaceaeAcidaminococcus
ProteomesiUP000001902 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotationSAAS annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

CytoplasmUniRule annotationSAAS annotation

Interactioni

Subunit structurei

Homodimer.UniRule annotationSAAS annotation

Protein-protein interaction databases

STRINGi591001.Acfer_1372.

Family & Domainsi

Domaini

Consists of three domains: the N-terminal catalytic domain, the editing domain and the C-terminal anticodon-binding domain.UniRule annotation

Sequence similaritiesi

Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 1 subfamily.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000076893.
KOiK01881.
OMAiIQPAELW.

Family and domain databases

Gene3Di3.40.50.800. 1 hit.
3.90.960.10. 1 hit.
HAMAPiMF_01569. Pro_tRNA_synth_type1.
InterProiIPR002314. aa-tRNA-synt_IIb_cons-dom.
IPR006195. aa-tRNA-synth_II.
IPR004154. Anticodon-bd.
IPR002316. Pro-tRNA-ligase_IIa.
IPR004500. Pro-tRNA-synth_IIa_bac-type.
IPR023717. Pro-tRNA-Synthase_IIa_type1.
IPR007214. YbaK/aa-tRNA-synth-assoc-dom.
[Graphical view]
PANTHERiPTHR11451:SF3. PTHR11451:SF3. 1 hit.
PfamiPF03129. HGTP_anticodon. 1 hit.
PF00587. tRNA-synt_2b. 1 hit.
PF04073. tRNA_edit. 1 hit.
[Graphical view]
PRINTSiPR01046. TRNASYNTHPRO.
SUPFAMiSSF52954. SSF52954. 1 hit.
SSF55826. SSF55826. 1 hit.
TIGRFAMsiTIGR00409. proS_fam_II. 1 hit.
PROSITEiPS50862. AA_TRNA_LIGASE_II. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

D2RKY0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRVSQLYAPT LREVPAEAVI ESHKLMLRAG YIRKVAGGLY SYLPLAWRSL
60 70 80 90 100
RKIEEIIREE MVPTGAQEIM MPIVQPSEIW QESGRWPAYG AEMFKLKDRH
110 120 130 140 150
GRDFCLGPTH EELVTTLVRD DLRSYRQLPV TLYQIQSKFR DEKRPRFGLM
160 170 180 190 200
RSREFIMKDA YSFDKDAEGL DKSYQDEYDA YSRIFTRCGL DYRPVEADSG
210 220 230 240 250
AIGGKGSHEF MALADSGEAG IVYCDTCEYA ADVEKAECAA IEAPAEEPKE
260 270 280 290 300
LEKKATPDCS TIEAVCQYLG SPIEKSVKAV AFVTDEGKLV LCFVRGDKEV
310 320 330 340 350
NDTKVVNAVG CNEVDMAPDD LIREAGTVPG FMGPIGLNKD KAIILIDHTV
360 370 380 390 400
MHMHNVCCGA NEKDVHYVNA EPSRDFVYTQ VADISTAVAG DKCPHCDGHL
410 420 430 440 450
KEARGIEVGQ VFKLNTKYSE ALHATYLDVD GKEHPLVMGC YGIGVGRTLA
460 470 480 490 500
AAIEQHHDKD GIIMPRNIAP YQVMVLAMNV KDEESWQKAV EIHDALNQAG
510 520 530 540 550
IDTLLDDRDE RAGVKFKDAD LIGCPLRIVI GPKTMKRNAM ETKLRCNGEM
560 570
ADVSFEGDWI QAIRDILDKA L
Length:571
Mass (Da):63,702
Last modified:March 2, 2010 - v1
Checksum:i7BE96F8A2F657E42
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001859 Genomic DNA. Translation: ADB47732.1.
RefSeqiWP_012938717.1. NC_013740.1.

Genome annotation databases

EnsemblBacteriaiADB47732; ADB47732; Acfer_1372.
KEGGiafn:Acfer_1372.
PATRICi31909273. VBIAciFer109666_1348.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001859 Genomic DNA. Translation: ADB47732.1.
RefSeqiWP_012938717.1. NC_013740.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi591001.Acfer_1372.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiADB47732; ADB47732; Acfer_1372.
KEGGiafn:Acfer_1372.
PATRICi31909273. VBIAciFer109666_1348.

Phylogenomic databases

HOGENOMiHOG000076893.
KOiK01881.
OMAiIQPAELW.

Enzyme and pathway databases

BioCyciAFER591001:GHUL-1427-MONOMER.

Family and domain databases

Gene3Di3.40.50.800. 1 hit.
3.90.960.10. 1 hit.
HAMAPiMF_01569. Pro_tRNA_synth_type1.
InterProiIPR002314. aa-tRNA-synt_IIb_cons-dom.
IPR006195. aa-tRNA-synth_II.
IPR004154. Anticodon-bd.
IPR002316. Pro-tRNA-ligase_IIa.
IPR004500. Pro-tRNA-synth_IIa_bac-type.
IPR023717. Pro-tRNA-Synthase_IIa_type1.
IPR007214. YbaK/aa-tRNA-synth-assoc-dom.
[Graphical view]
PANTHERiPTHR11451:SF3. PTHR11451:SF3. 1 hit.
PfamiPF03129. HGTP_anticodon. 1 hit.
PF00587. tRNA-synt_2b. 1 hit.
PF04073. tRNA_edit. 1 hit.
[Graphical view]
PRINTSiPR01046. TRNASYNTHPRO.
SUPFAMiSSF52954. SSF52954. 1 hit.
SSF55826. SSF55826. 1 hit.
TIGRFAMsiTIGR00409. proS_fam_II. 1 hit.
PROSITEiPS50862. AA_TRNA_LIGASE_II. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 25085 / DSM 20731 / VR4Imported.

Entry informationi

Entry nameiD2RKY0_ACIFV
AccessioniPrimary (citable) accession number: D2RKY0
Entry historyi
Integrated into UniProtKB/TrEMBL: March 2, 2010
Last sequence update: March 2, 2010
Last modified: July 22, 2015
This is version 44 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteomeImported

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.