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D2RFU2 (D2RFU2_ARCPA) Unreviewed, UniProtKB/TrEMBL

Last modified May 1, 2013. Version 30. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
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Protein attributes

Sequence length632 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

DNA gyrase negatively supercoils closed circular double-stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings By similarity. HAMAP-Rule MF_01898 RuleBase RU003363

Catalytic activity

ATP-dependent breakage, passage and rejoining of double-stranded DNA. HAMAP-Rule MF_01898 RuleBase RU003363

Cofactor

Magnesium. Binds two Mg2+ per subunit. The magnesium ions form salt bridges with both the protein and the DNA. Can also accept other divalent metal cations, such as Mn2+ and Ca2+ By similarity. HAMAP-Rule MF_01898

Subunit structure

Heterotetramer, composed of two GyrA and two GyrB chains. Within the heterotetramer, GyrA contains the active site tyrosine that forms a covalent intermediate with the DNA, while GyrB contributes the cofactor binding sites and catalyzes ATP hydrolysis By similarity. HAMAP-Rule MF_01898

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_01898.

Sequence similarities

Belongs to the type II topoisomerase family. HAMAP-Rule MF_01898 RuleBase RU000380

Contains 1 Toprim domain. HAMAP-Rule MF_01898

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Regions

Domain419 – 533115Toprim By similarity HAMAP-Rule MF_01898

Sites

Metal binding4251Magnesium 1; catalytic By similarity HAMAP-Rule MF_01898
Metal binding4981Magnesium 1; catalytic By similarity HAMAP-Rule MF_01898
Metal binding4981Magnesium 2 By similarity HAMAP-Rule MF_01898
Metal binding5001Magnesium 2 By similarity HAMAP-Rule MF_01898
Site4501Interaction with DNA By similarity HAMAP-Rule MF_01898
Site4531Interaction with DNA By similarity HAMAP-Rule MF_01898

Sequences

Sequence LengthMass (Da)Tools
D2RFU2 [UniParc].

Last modified March 2, 2010. Version 1.
Checksum: FE241AE80D8F7551

FASTA63271,239
        10         20         30         40         50         60 
MPISSTFLAM SDYTAEAIEV LDDIQAVRKR PGMYVGSTGS RGFHHLLWEI VDNSIDEALA 

        70         80         90        100        110        120 
GYCNRIVVTL HKDGSASVED NGRGIPVEEH PIFKKPALEI VMTKLHAGGK FSKKAYKVSG 

       130        140        150        160        170        180 
GLHGVGLSVV NALSEWLEVW VKRNGKIYYQ RYERGKPVTP LKVVGETKET GTKIRFKPDP 

       190        200        210        220        230        240 
EIFEVKEFDY DVVAERLREL AFLNKGLRIK LIDERTGKVK EFYSEEGIVG FVKYLNKGKD 

       250        260        270        280        290        300 
VITDVVYISG EKNGINVEIA FQFTNEDFEN LLAFANNIHT IEGGTHVSGF RAGLTRAINE 

       310        320        330        340        350        360 
YGKKHVKKFT PISGSDIREG LTAVLSVKVP EPQFEGQTKT KLTNSDVKTV VESLVYNNFL 

       370        380        390        400        410        420 
RWLEEHPSEA QRIIEKCLLA MKAREMARRA RELVKRKAEI SLTLPGKLAD CSSKNVEERE 

       430        440        450        460        470        480 
LFIVEGESAG GSAKQARDRR FQAVLPIRGK IINVEKAGML RALKNEEIKA IASAIGGGIG 

       490        500        510        520        530        540 
KDFDLSKVRY GKIIIMTDAD VDGAHIRTLL LTFFYRYMRP LIEAGMVYIA LPPLYRVKKG 

       550        560        570        580        590        600 
DKVYYVYSEK ELRDVLKKIG KAEVQRFKGL GEMNPEQLWE TAMNPKTRKL VKVTIEDALI 

       610        620        630 
AEKMFRILMG ENVDERKEFI LNHAKEVKNL DV

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Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP001857 Genomic DNA. Translation: ADB57167.1.
RefSeqYP_003399840.1. NC_013741.1.

3D structure databases

ProteinModelPortalD2RFU2.
ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaADB57167; ADB57167; Arcpr_0091.
GeneID8738737.
KEGGapo:Arcpr_0091.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMHOG000075156.
KOK02470.
OMAIFETTEF.

Enzyme and pathway databases

BioCycAPRO572546:GJI1-93-MONOMER.

Family and domain databases

Gene3D3.30.230.10. 1 hit.
3.30.565.10. 1 hit.
3.40.50.670. 1 hit.
HAMAPMF_01898. GyrB.
InterProIPR002288. DNA_gyrase_B_C.
IPR011557. GyrB.
IPR003594. HATPase_ATP-bd.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR014721. Ribosomal_S5_D2-typ_fold_subgr.
IPR001241. Topo_IIA.
IPR013506. Topo_IIA_bsu_dom2.
IPR013759. Topo_IIA_cen_dom.
IPR013760. Topo_IIA_like_dom.
IPR006171. Toprim_domain.
[Graphical view]
PfamPF00204. DNA_gyraseB. 1 hit.
PF00986. DNA_gyraseB_C. 1 hit.
PF02518. HATPase_c. 1 hit.
PF01751. Toprim. 1 hit.
[Graphical view]
PRINTSPR00418. TPI2FAMILY.
SMARTSM00387. HATPase_c. 1 hit.
SM00433. TOP2c. 1 hit.
[Graphical view]
SUPFAMSSF55874. ATP_bd_ATPase. 1 hit.
SSF54211. Ribosomal_S5_D2-typ_fold. 1 hit.
SSF56719. Topo_IIA_cen. 1 hit.
TIGRFAMsTIGR01059. gyrB. 1 hit.
ProtoNetSearch...

Entry information

Entry nameD2RFU2_ARCPA
AccessionPrimary (citable) accession number: D2RFU2
Entry history
Integrated into UniProtKB/TrEMBL: March 2, 2010
Last sequence update: March 2, 2010
Last modified: May 1, 2013
This is version 30 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)
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