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D2RC91 (D2RC91_GARV4) Unreviewed, UniProtKB/TrEMBL

Last modified June 11, 2014. Version 33. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry infoCustomize order

Names and origin

Protein namesRecommended name:
D-alanine--D-alanine ligase HAMAP-Rule MF_00047

EC=6.3.2.4 HAMAP-Rule MF_00047
Alternative name(s):
D-Ala-D-Ala ligase HAMAP-Rule MF_00047
D-alanylalanine synthetase HAMAP-Rule MF_00047
Gene names
Name:ddl HAMAP-Rule MF_00047
Ordered Locus Names:HMPREF0424_1233 EMBL ADB14041.1
OrganismGardnerella vaginalis (strain 409-05) [Complete proteome] [HAMAP] EMBL ADB14041.1
Taxonomic identifier553190 [NCBI]
Taxonomic lineageBacteriaActinobacteriaActinobacteridaeBifidobacterialesBifidobacteriaceaeGardnerella

Protein attributes

Sequence length380 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Cell wall formation By similarity. HAMAP-Rule MF_00047 SAAS SAAS011095

Catalytic activity

ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine. HAMAP-Rule MF_00047 SAAS SAAS011095

Cofactor

Binds 2 magnesium or manganese ions per subunit By similarity. HAMAP-Rule MF_00047 SAAS SAAS011095

Pathway

Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP-Rule MF_00047 SAAS SAAS011095

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00047 SAAS SAAS011095.

Sequence similarities

Belongs to the D-alanine--D-alanine ligase family. HAMAP-Rule MF_00047

Contains 1 ATP-grasp domain. HAMAP-Rule MF_00047

Contains ATP-grasp domain. SAAS SAAS011095

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Regions

Domain150 – 376227ATP-grasp By similarity HAMAP-Rule MF_00047
Nucleotide binding192 – 24958ATP By similarity HAMAP-Rule MF_00047

Sites

Metal binding3301Magnesium or manganese 1 By similarity HAMAP-Rule MF_00047
Metal binding3431Magnesium or manganese 1 By similarity HAMAP-Rule MF_00047
Metal binding3431Magnesium or manganese 2 By similarity HAMAP-Rule MF_00047
Metal binding3451Magnesium or manganese 2 By similarity HAMAP-Rule MF_00047

Sequences

Sequence LengthMass (Da)Tools
D2RC91 [UniParc].

Last modified March 2, 2010. Version 1.
Checksum: 4A0BE9B630852FAA

FASTA38041,170
        10         20         30         40         50         60 
MTKKRVVFLY GGKADEHSIS CISAAGVLSA VDENRFDIVR IAITKQGEWI VGGEDPRNFH 

        70         80         90        100        110        120 
MEEGELPVIT KTAGTRDVVL DPAKGADGFL ARENDGTLTS LGHIDAVFPV LHGPNGEDGT 

       130        140        150        160        170        180 
LQGLLEMMQV PYVGCGVLAS AACMDKYYAK QLFKAAGIDV APGIALDARK FASDAENRFD 

       190        200        210        220        230        240 
AYAEEILAQV EAAKLEYPLF VKPSRAGSSF GVTKVESRDA KALAEAIFEA SEHDWRVLVE 

       250        260        270        280        290        300 
QGIDAREIEC AVLAARDGEE PKASWPGEVV LDKREAGEDQ FYDFDSKYMD SAASHVEVPA 

       310        320        330        340        350        360 
NLPAETLERV RKTALAAFKA ADGRGLSRVD SFVTKDGRVI LNEINTMPGF TPISMYPKAW 

       370        380 
EATGISYSDL ITKLIEGVLK 

« Hide

References

[1]"Comparative genomics of Gardnerella vaginalis strains reveals substantial differences in metabolic and virulence potential."
Yeoman C.J., Yildirim S., Thomas S.M., Durkin A.S., Torralba M., Sutton G., Buhay C.J., Ding Y., Dugan-Rocha S.P., Muzny D.M., Qin X., Gibbs R.A., Leigh S.R., Stumpf R., White B.A., Highlander S.K., Nelson K.E., Wilson B.A.
PLoS ONE 5:E12411-E12411(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 409-05.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP001849 Genomic DNA. Translation: ADB14041.1.
RefSeqYP_003374426.1. NC_013721.1.

3D structure databases

ProteinModelPortalD2RC91.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaADB14041; ADB14041; HMPREF0424_1233.
GeneID8709920.
KEGGgva:HMPREF0424_1233.
PATRIC32148266. VBIGarVag136958_1087.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMHOG000011593.
KOK01921.
OMAIECGVLG.
OrthoDBEOG64BQ73.

Enzyme and pathway databases

BioCycGVAG553190:GJPT-1227-MONOMER.
UniPathwayUPA00219.

Family and domain databases

Gene3D3.30.1490.20. 1 hit.
3.30.470.20. 2 hits.
3.40.50.20. 1 hit.
HAMAPMF_00047. Dala_Dala_lig.
InterProIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR000291. D-Ala_lig_Van_CS.
IPR005905. D_ala_D_ala.
IPR011095. Dala_Dala_lig_C.
IPR011127. Dala_Dala_lig_N.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PANTHERPTHR23132. PTHR23132. 1 hit.
PfamPF07478. Dala_Dala_lig_C. 1 hit.
PF01820. Dala_Dala_lig_N. 1 hit.
[Graphical view]
SUPFAMSSF52440. SSF52440. 1 hit.
TIGRFAMsTIGR01205. D_ala_D_alaTIGR. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
PS00843. DALA_DALA_LIGASE_1. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameD2RC91_GARV4
AccessionPrimary (citable) accession number: D2RC91
Entry history
Integrated into UniProtKB/TrEMBL: March 2, 2010
Last sequence update: March 2, 2010
Last modified: June 11, 2014
This is version 33 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)