D2QQG4 (D2QQG4_SPILD) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
Isocitrate dehydrogenase [NADP] PIRNR PIRNR000108
EC=1.1.1.42 PIRNR PIRNR000108

Gene names

Ordered Locus Names:

Slin_4767 EMBL ADB40745.1

Organism

Spirosoma linguale (strain ATCC 33905 / DSM 74 / LMG 10896) [Complete proteome] [HAMAP] EMBL ADB40745.1

Taxonomic identifier

504472 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Bacteroidetes/Chlorobi group › Bacteroidetes › Cytophagia › Cytophagales › Cytophagaceae › Spirosoma › Spirosoma linguale

Protein attributes

Sequence length	405 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Isocitrate + NADP+ = 2-oxoglutarate + CO2 + NADPH. PIRNR PIRNR000108
Cofactor	Binds 1 magnesium or manganese ion per subunit By similarity. PIRNR PIRNR000108 PIRSR PIRSR000108-3
Sequence similarities	Belongs to the isocitrate and isopropylmalate dehydrogenases family. PIRNR PIRNR000108 RuleBase RU004443

Ontologies

Keywords
Biological process	Tricarboxylic acid cycle PIRNR PIRNR000108
Ligand	Magnesium PIRNR PIRNR000108 PIRSR PIRSR000108-3 Manganese PIRNR PIRNR000108 PIRSR PIRSR000108-3 Metal-binding PIRNR PIRNR000108 PIRSR PIRSR000108-3 NADP PIRNR PIRNR000108 PIRSR PIRSR000108-4
Molecular function	Oxidoreductase PIRNR PIRNR000108 RuleBase RU004444 EMBL ADB40745.1
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	isocitrate metabolic process Inferred from electronic annotation. Source: InterPro tricarboxylic acid cycle Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	NAD binding Inferred from electronic annotation. Source: InterPro isocitrate dehydrogenase (NADP+) activity Inferred from electronic annotation. Source: EC magnesium ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Regions
	Nucleotide binding	75 – 77	3	NADP By similarity PIRSR PIRSR000108-4
	Nucleotide binding	303 – 308	6	NADP By similarity PIRSR PIRSR000108-4
	Region	94 – 100	7	Substrate binding By similarity PIRSR PIRSR000108-2
Sites
	Metal binding	245	1	Magnesium or manganese By similarity PIRSR PIRSR000108-3
	Metal binding	268	1	Magnesium or manganese By similarity PIRSR PIRSR000108-3
	Binding site	77	1	Substrate By similarity PIRSR PIRSR000108-2
	Binding site	82	1	NADP By similarity PIRSR PIRSR000108-4
	Binding site	109	1	Substrate By similarity PIRSR PIRSR000108-2
	Binding site	132	1	Substrate By similarity PIRSR PIRSR000108-2
	Binding site	253	1	NADP By similarity PIRSR PIRSR000108-4
	Binding site	321	1	NADP; via amide nitrogen and carbonyl oxygen By similarity PIRSR PIRSR000108-4
	Site	139	1	Critical for catalysis By similarity PIRSR PIRSR000108-1
	Site	210	1	Critical for catalysis By similarity PIRSR PIRSR000108-1

Sequences

	Sequence		Length	Mass (Da)
	D2QQG4 [UniParc]. Last modified March 2, 2010. Version 1. Checksum: D6A889B983932A49	FASTA	405	45,537
	10 20 30 40 50 60 MEKIKVANPV VELDGDEMTR IIWKFIKDKL ILPYLDLDIK YYDLGIEYRD ETNDQVTIDA 70 80 90 100 110 120 ANAIKEYGVG IKCATITPDE DRVKEFNLKQ MWKSPNGTIR NILDGTVFRE PIVMNNVPRL 130 140 150 160 170 180 VTNWKAPIIV GRHAFGDQYR ATDFLVPGKG KLTMKFEGED GTVLEYDVFN FPGAGVAMGM 190 200 210 220 230 240 YNLDESIRGF ARACFNMALQ KNWPLYLSTK NTILKKYDGR FKDIFQEIYD AEFAGKVHYE 250 260 270 280 290 300 HRLIDDMVAS ALKWEGNFVW ACKNYDGDVQ SDTVAQGFGS LGLMTSVLVT PDGKTMEAEA 310 320 330 340 350 360 AHGTVTRHYR EYQKGNKTST NPIASIYAWT RGLAFRGQLD GNQPLIDFAN ALEAVCVETV 370 380 390 400 ESGKMTKDLA LSAYPVGTKL TAGEHYLNTE DFLEALDTNL QAKLA

References

[1]

"The complete chromosome of Spirosoma linguale DSM 74." US DOE Joint Genome Institute (JGI-PGF) Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Kyrpides N., Mavromatis K., Mikhailova N., Ovchinnikova G., Saunders E., Brettin T., Detter J.C., Han C., Larimer F. , Land M., Hauser L., Markowitz V., Cheng J.-F., Hugenholtz P., Woyke T., Wu D., Tindal B., Schutze A., Schneider S., Goker M., Klenk H.-P., Eisen J.A. Submitted (SEP-2009) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 33905 / DSM 74 / LMG 10896.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001769 Genomic DNA. Translation: ADB40745.1.
RefSeq	YP_003389544.1. NC_013730.1.
3D structure databases
ProteinModelPortal	D2QQG4.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ADB40745; ADB40745; Slin_4767.
GeneID	8728531.
KEGG	sli:Slin_4767.
PATRIC	32439483. VBISpiLin97822_4764.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HOG000019858.
KO	K00031.
OMA	QHQQGKP.
Enzyme and pathway databases
BioCyc	SLIN504472:GHKB-5234-MONOMER.
Family and domain databases
Gene3D	3.40.718.10. 1 hit.
InterPro	IPR019818. IsoCit/isopropylmalate_DH_CS. IPR004790. Isocitrate_DH_NADP. IPR024084. IsoPropMal-DH-like_dom. [Graphical view]
PANTHER	PTHR11822. PTHR11822. 1 hit.
Pfam	PF00180. Iso_dh. 1 hit. [Graphical view]
PIRSF	PIRSF000108. IDH_NADP. 1 hit.
TIGRFAMs	TIGR00127. nadp_idh_euk. 1 hit.
PROSITE	PS00470. IDH_IMDH. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

D2QQG4_SPILD

Accession

Primary (citable) accession number: D2QQG4

Entry history

Integrated into UniProtKB/TrEMBL:	March 2, 2010
Last sequence update:	March 2, 2010
Last modified:	May 1, 2013
This is version 31 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)