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D2QEF5

- D2QEF5_SPILD

UniProt

D2QEF5 - D2QEF5_SPILD

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Protein
Lipoyl synthase
Gene
lipA, Slin_0446
Organism
Spirosoma linguale (strain ATCC 33905 / DSM 74 / LMG 10896)
Status
Unreviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives By similarity.UniRule annotation

Catalytic activityi

Protein N(6)-(octanoyl)lysine + 2 sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = protein N(6)-(lipoyl)lysine + 2 (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.UniRule annotation

Cofactori

Binds 2 4Fe-4S clusters per subunit. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi56 – 561Iron-sulfur 1 (4Fe-4S) By similarityUniRule annotation
Metal bindingi61 – 611Iron-sulfur 1 (4Fe-4S) By similarityUniRule annotation
Metal bindingi67 – 671Iron-sulfur 1 (4Fe-4S) By similarityUniRule annotation
Metal bindingi82 – 821Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarityUniRule annotation
Metal bindingi86 – 861Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarityUniRule annotation
Metal bindingi89 – 891Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarityUniRule annotation

GO - Molecular functioni

  1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-HAMAP
  2. lipoate synthase activity Source: UniProtKB-HAMAP
  3. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. protein lipoylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

TransferaseUniRule annotationImported

Keywords - Ligandi

4Fe-4SUniRule annotation, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionineUniRule annotation

Enzyme and pathway databases

BioCyciSLIN504472:GHKB-446-MONOMER.
UniPathwayiUPA00538; UER00593.

Names & Taxonomyi

Protein namesi
Recommended name:
Lipoyl synthaseUniRule annotation (EC:2.8.1.8UniRule annotation)
Alternative name(s):
Lip-syn
Lipoate synthase
Lipoic acid synthase
Sulfur insertion protein LipA
Gene namesi
Name:lipAUniRule annotation
Ordered Locus Names:Slin_0446Imported
OrganismiSpirosoma linguale (strain ATCC 33905 / DSM 74 / LMG 10896)Imported
Taxonomic identifieri504472 [NCBI]
Taxonomic lineageiBacteriaBacteroidetesCytophagiaCytophagalesCytophagaceaeSpirosoma
ProteomesiUP000002028: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

CytoplasmUniRule annotation

Structurei

3D structure databases

ProteinModelPortaliD2QEF5.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

HOGENOMiHOG000235997.
KOiK03644.
OMAiPNIAECF.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_00206. Lipoyl_synth.
InterProiIPR013785. Aldolase_TIM.
IPR006638. Elp3/MiaB/NifB.
IPR003698. Lipoyl_synth.
IPR007197. rSAM.
[Graphical view]
PANTHERiPTHR10949. PTHR10949. 1 hit.
PfamiPF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFiPIRSF005963. Lipoyl_synth. 1 hit.
SMARTiSM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00510. lipA. 1 hit.

Sequencei

Sequence statusi: Complete.

D2QEF5-1 [UniParc]FASTAAdd to Basket

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MIELPVIPSE QQRNRAGADA APRPKRPDWL RVKLPIGPEY AKVRKLVDEH    50
KLHTICESGN CPNMGECWGA GTATFMILGN VCTRSCTFCA VATGRPNEYD 100
TDEPRRVAEA IVLMKVKHAV LTSVNRDELK DRGAEIWYQT VRLIKEASPA 150
TTIETLIPDT KGNWEALERM ISAGQEVVSH NMETVERLYR RVRPQARYER 200
SLEQIRRTKD YGQRTKSGIM LGLGETKDEV VKAMDDLAAN GLDILTLGQY 250
LQPTKMHHEV IEWIHPETFA MYREEGLRRG LKYVESGPLV RSSYHAEKHV 300
NV 302
Length:302
Mass (Da):34,321
Last modified:March 2, 2010 - v1
Checksum:iF74A0C77BC178855
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP001769 Genomic DNA. Translation: ADB36510.1.
RefSeqiYP_003385309.1. NC_013730.1.

Genome annotation databases

EnsemblBacteriaiADB36510; ADB36510; Slin_0446.
GeneIDi8724174.
KEGGisli:Slin_0446.
PATRICi32430793. VBISpiLin97822_0453.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP001769 Genomic DNA. Translation: ADB36510.1 .
RefSeqi YP_003385309.1. NC_013730.1.

3D structure databases

ProteinModelPortali D2QEF5.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ADB36510 ; ADB36510 ; Slin_0446 .
GeneIDi 8724174.
KEGGi sli:Slin_0446.
PATRICi 32430793. VBISpiLin97822_0453.

Phylogenomic databases

HOGENOMi HOG000235997.
KOi K03644.
OMAi PNIAECF.

Enzyme and pathway databases

UniPathwayi UPA00538 ; UER00593 .
BioCyci SLIN504472:GHKB-446-MONOMER.

Family and domain databases

Gene3Di 3.20.20.70. 1 hit.
HAMAPi MF_00206. Lipoyl_synth.
InterProi IPR013785. Aldolase_TIM.
IPR006638. Elp3/MiaB/NifB.
IPR003698. Lipoyl_synth.
IPR007197. rSAM.
[Graphical view ]
PANTHERi PTHR10949. PTHR10949. 1 hit.
Pfami PF04055. Radical_SAM. 1 hit.
[Graphical view ]
PIRSFi PIRSF005963. Lipoyl_synth. 1 hit.
SMARTi SM00729. Elp3. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR00510. lipA. 1 hit.
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 33905 / DSM 74 / LMG 10896.

Entry informationi

Entry nameiD2QEF5_SPILD
AccessioniPrimary (citable) accession number: D2QEF5
Entry historyi
Integrated into UniProtKB/TrEMBL: March 2, 2010
Last sequence update: March 2, 2010
Last modified: June 11, 2014
This is version 35 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome

External Data

Dasty 3

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