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D2QEF5

- D2QEF5_SPILD

UniProt

D2QEF5 - D2QEF5_SPILD

Protein

Lipoyl synthase

Gene

lipA

Organism
Spirosoma linguale (strain ATCC 33905 / DSM 74 / LMG 10896)
Status
Unreviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 36 (01 Oct 2014)
      Sequence version 1 (02 Mar 2010)
      Previous versions | rss
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    Functioni

    Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives.UniRule annotation

    Catalytic activityi

    Protein N(6)-(octanoyl)lysine + 2 sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = protein N(6)-(lipoyl)lysine + 2 (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.UniRule annotation

    Cofactori

    Binds 2 4Fe-4S clusters per subunit. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi56 – 561Iron-sulfur 1 (4Fe-4S)UniRule annotation
    Metal bindingi61 – 611Iron-sulfur 1 (4Fe-4S)UniRule annotation
    Metal bindingi67 – 671Iron-sulfur 1 (4Fe-4S)UniRule annotation
    Metal bindingi82 – 821Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation
    Metal bindingi86 – 861Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation
    Metal bindingi89 – 891Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation

    GO - Molecular functioni

    1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-HAMAP
    2. lipoate synthase activity Source: UniProtKB-HAMAP
    3. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. protein lipoylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    TransferaseUniRule annotationImported

    Keywords - Ligandi

    4Fe-4SUniRule annotation, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionineUniRule annotation

    Enzyme and pathway databases

    BioCyciSLIN504472:GHKB-446-MONOMER.
    UniPathwayiUPA00538; UER00593.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Lipoyl synthaseUniRule annotation (EC:2.8.1.8UniRule annotation)
    Alternative name(s):
    Lip-synUniRule annotation
    Lipoate synthaseUniRule annotation
    Lipoic acid synthaseUniRule annotation
    Sulfur insertion protein LipAUniRule annotation
    Gene namesi
    Name:lipAUniRule annotation
    Ordered Locus Names:Slin_0446Imported
    OrganismiSpirosoma linguale (strain ATCC 33905 / DSM 74 / LMG 10896)Imported
    Taxonomic identifieri504472 [NCBI]
    Taxonomic lineageiBacteriaBacteroidetesCytophagiaCytophagalesCytophagaceaeSpirosoma
    ProteomesiUP000002028: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    CytoplasmUniRule annotation

    Structurei

    3D structure databases

    ProteinModelPortaliD2QEF5.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the radical SAM superfamily. Lipoyl synthase family.UniRule annotation

    Phylogenomic databases

    HOGENOMiHOG000235997.
    KOiK03644.
    OMAiPNIAECF.

    Family and domain databases

    Gene3Di3.20.20.70. 1 hit.
    HAMAPiMF_00206. Lipoyl_synth.
    InterProiIPR013785. Aldolase_TIM.
    IPR006638. Elp3/MiaB/NifB.
    IPR003698. Lipoyl_synth.
    IPR007197. rSAM.
    [Graphical view]
    PANTHERiPTHR10949. PTHR10949. 1 hit.
    PfamiPF04055. Radical_SAM. 1 hit.
    [Graphical view]
    PIRSFiPIRSF005963. Lipoyl_synth. 1 hit.
    SMARTiSM00729. Elp3. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR00510. lipA. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    D2QEF5-1 [UniParc]FASTAAdd to Basket

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    MIELPVIPSE QQRNRAGADA APRPKRPDWL RVKLPIGPEY AKVRKLVDEH    50
    KLHTICESGN CPNMGECWGA GTATFMILGN VCTRSCTFCA VATGRPNEYD 100
    TDEPRRVAEA IVLMKVKHAV LTSVNRDELK DRGAEIWYQT VRLIKEASPA 150
    TTIETLIPDT KGNWEALERM ISAGQEVVSH NMETVERLYR RVRPQARYER 200
    SLEQIRRTKD YGQRTKSGIM LGLGETKDEV VKAMDDLAAN GLDILTLGQY 250
    LQPTKMHHEV IEWIHPETFA MYREEGLRRG LKYVESGPLV RSSYHAEKHV 300
    NV 302
    Length:302
    Mass (Da):34,321
    Last modified:March 2, 2010 - v1
    Checksum:iF74A0C77BC178855
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP001769 Genomic DNA. Translation: ADB36510.1.
    RefSeqiYP_003385309.1. NC_013730.1.

    Genome annotation databases

    EnsemblBacteriaiADB36510; ADB36510; Slin_0446.
    GeneIDi8724174.
    KEGGisli:Slin_0446.
    PATRICi32430793. VBISpiLin97822_0453.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP001769 Genomic DNA. Translation: ADB36510.1 .
    RefSeqi YP_003385309.1. NC_013730.1.

    3D structure databases

    ProteinModelPortali D2QEF5.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ADB36510 ; ADB36510 ; Slin_0446 .
    GeneIDi 8724174.
    KEGGi sli:Slin_0446.
    PATRICi 32430793. VBISpiLin97822_0453.

    Phylogenomic databases

    HOGENOMi HOG000235997.
    KOi K03644.
    OMAi PNIAECF.

    Enzyme and pathway databases

    UniPathwayi UPA00538 ; UER00593 .
    BioCyci SLIN504472:GHKB-446-MONOMER.

    Family and domain databases

    Gene3Di 3.20.20.70. 1 hit.
    HAMAPi MF_00206. Lipoyl_synth.
    InterProi IPR013785. Aldolase_TIM.
    IPR006638. Elp3/MiaB/NifB.
    IPR003698. Lipoyl_synth.
    IPR007197. rSAM.
    [Graphical view ]
    PANTHERi PTHR10949. PTHR10949. 1 hit.
    Pfami PF04055. Radical_SAM. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF005963. Lipoyl_synth. 1 hit.
    SMARTi SM00729. Elp3. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR00510. lipA. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 33905 / DSM 74 / LMG 10896Imported.

    Entry informationi

    Entry nameiD2QEF5_SPILD
    AccessioniPrimary (citable) accession number: D2QEF5
    Entry historyi
    Integrated into UniProtKB/TrEMBL: March 2, 2010
    Last sequence update: March 2, 2010
    Last modified: October 1, 2014
    This is version 36 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiUnreviewed (UniProtKB/TrEMBL)

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteomeImported

    External Data

    Dasty 3