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D2QEF5

- D2QEF5_SPILD

UniProt

D2QEF5 - D2QEF5_SPILD

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Protein

Lipoyl synthase

Gene

lipA

Organism
Spirosoma linguale (strain ATCC 33905 / DSM 74 / LMG 10896)
Status
Unreviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives.UniRule annotation

Catalytic activityi

Protein N(6)-(octanoyl)lysine + 2 sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = protein N(6)-(lipoyl)lysine + 2 (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.UniRule annotation

Cofactori

Binds 2 4Fe-4S clusters per subunit. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi56 – 561Iron-sulfur 1 (4Fe-4S)UniRule annotation
Metal bindingi61 – 611Iron-sulfur 1 (4Fe-4S)UniRule annotation
Metal bindingi67 – 671Iron-sulfur 1 (4Fe-4S)UniRule annotation
Metal bindingi82 – 821Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation
Metal bindingi86 – 861Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation
Metal bindingi89 – 891Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation

GO - Molecular functioni

  1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-HAMAP
  2. lipoate synthase activity Source: UniProtKB-HAMAP
  3. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. protein lipoylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

TransferaseUniRule annotationImported

Keywords - Ligandi

4Fe-4SUniRule annotation, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionineUniRule annotation

Enzyme and pathway databases

BioCyciSLIN504472:GHKB-446-MONOMER.
UniPathwayiUPA00538; UER00593.

Names & Taxonomyi

Protein namesi
Recommended name:
Lipoyl synthaseUniRule annotation (EC:2.8.1.8UniRule annotation)
Alternative name(s):
Lip-synUniRule annotation
Lipoate synthaseUniRule annotation
Lipoic acid synthaseUniRule annotation
Sulfur insertion protein LipAUniRule annotation
Gene namesi
Name:lipAUniRule annotation
Ordered Locus Names:Slin_0446Imported
OrganismiSpirosoma linguale (strain ATCC 33905 / DSM 74 / LMG 10896)Imported
Taxonomic identifieri504472 [NCBI]
Taxonomic lineageiBacteriaBacteroidetesCytophagiaCytophagalesCytophagaceaeSpirosoma
ProteomesiUP000002028: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

CytoplasmUniRule annotation

Structurei

3D structure databases

ProteinModelPortaliD2QEF5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the radical SAM superfamily. Lipoyl synthase family.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000235997.
KOiK03644.
OMAiPNIAECF.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_00206. Lipoyl_synth.
InterProiIPR013785. Aldolase_TIM.
IPR006638. Elp3/MiaB/NifB.
IPR003698. Lipoyl_synth.
IPR007197. rSAM.
[Graphical view]
PANTHERiPTHR10949. PTHR10949. 1 hit.
PfamiPF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFiPIRSF005963. Lipoyl_synth. 1 hit.
SMARTiSM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00510. lipA. 1 hit.

Sequencei

Sequence statusi: Complete.

D2QEF5-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MIELPVIPSE QQRNRAGADA APRPKRPDWL RVKLPIGPEY AKVRKLVDEH
60 70 80 90 100
KLHTICESGN CPNMGECWGA GTATFMILGN VCTRSCTFCA VATGRPNEYD
110 120 130 140 150
TDEPRRVAEA IVLMKVKHAV LTSVNRDELK DRGAEIWYQT VRLIKEASPA
160 170 180 190 200
TTIETLIPDT KGNWEALERM ISAGQEVVSH NMETVERLYR RVRPQARYER
210 220 230 240 250
SLEQIRRTKD YGQRTKSGIM LGLGETKDEV VKAMDDLAAN GLDILTLGQY
260 270 280 290 300
LQPTKMHHEV IEWIHPETFA MYREEGLRRG LKYVESGPLV RSSYHAEKHV

NV
Length:302
Mass (Da):34,321
Last modified:March 2, 2010 - v1
Checksum:iF74A0C77BC178855
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP001769 Genomic DNA. Translation: ADB36510.1.
RefSeqiWP_012925062.1. NC_013730.1.
YP_003385309.1. NC_013730.1.

Genome annotation databases

EnsemblBacteriaiADB36510; ADB36510; Slin_0446.
GeneIDi8724174.
KEGGisli:Slin_0446.
PATRICi32430793. VBISpiLin97822_0453.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP001769 Genomic DNA. Translation: ADB36510.1 .
RefSeqi WP_012925062.1. NC_013730.1.
YP_003385309.1. NC_013730.1.

3D structure databases

ProteinModelPortali D2QEF5.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ADB36510 ; ADB36510 ; Slin_0446 .
GeneIDi 8724174.
KEGGi sli:Slin_0446.
PATRICi 32430793. VBISpiLin97822_0453.

Phylogenomic databases

HOGENOMi HOG000235997.
KOi K03644.
OMAi PNIAECF.

Enzyme and pathway databases

UniPathwayi UPA00538 ; UER00593 .
BioCyci SLIN504472:GHKB-446-MONOMER.

Family and domain databases

Gene3Di 3.20.20.70. 1 hit.
HAMAPi MF_00206. Lipoyl_synth.
InterProi IPR013785. Aldolase_TIM.
IPR006638. Elp3/MiaB/NifB.
IPR003698. Lipoyl_synth.
IPR007197. rSAM.
[Graphical view ]
PANTHERi PTHR10949. PTHR10949. 1 hit.
Pfami PF04055. Radical_SAM. 1 hit.
[Graphical view ]
PIRSFi PIRSF005963. Lipoyl_synth. 1 hit.
SMARTi SM00729. Elp3. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR00510. lipA. 1 hit.
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 33905 / DSM 74 / LMG 10896Imported.

Entry informationi

Entry nameiD2QEF5_SPILD
AccessioniPrimary (citable) accession number: D2QEF5
Entry historyi
Integrated into UniProtKB/TrEMBL: March 2, 2010
Last sequence update: March 2, 2010
Last modified: October 29, 2014
This is version 37 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteomeImported

External Data

Dasty 3