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D2QEF5 (D2QEF5_SPILD) Unreviewed, UniProtKB/TrEMBL

Last modified February 19, 2014. Version 33. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Lipoyl synthase HAMAP-Rule MF_00206

EC=2.8.1.8 HAMAP-Rule MF_00206
Alternative name(s):
Lip-syn HAMAP-Rule MF_00206
Lipoate synthase HAMAP-Rule MF_00206
Lipoic acid synthase HAMAP-Rule MF_00206
Sulfur insertion protein LipA HAMAP-Rule MF_00206
Gene names
Name:lipA HAMAP-Rule MF_00206
Ordered Locus Names:Slin_0446 EMBL ADB36510.1
OrganismSpirosoma linguale (strain ATCC 33905 / DSM 74 / LMG 10896) [Complete proteome] [HAMAP] EMBL ADB36510.1
Taxonomic identifier504472 [NCBI]
Taxonomic lineageBacteriaBacteroidetesCytophagiaCytophagalesCytophagaceaeSpirosoma

Protein attributes

Sequence length302 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives By similarity. HAMAP-Rule MF_00206

Catalytic activity

Protein N(6)-(octanoyl)lysine + 2 sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = protein N(6)-(lipoyl)lysine + 2 (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine. HAMAP-Rule MF_00206

Cofactor

Binds 2 4Fe-4S clusters per subunit. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity. HAMAP-Rule MF_00206

Pathway

Protein modification; protein lipoylation via endogenous pathway; protein N(6)-(lipoyl)lysine from octanoyl-[acyl-carrier-protein]: step 2/2. HAMAP-Rule MF_00206

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00206.

Sequence similarities

Belongs to the radical SAM superfamily. Lipoyl synthase family. HAMAP-Rule MF_00206

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Sites

Metal binding561Iron-sulfur 1 (4Fe-4S) By similarity HAMAP-Rule MF_00206
Metal binding611Iron-sulfur 1 (4Fe-4S) By similarity HAMAP-Rule MF_00206
Metal binding671Iron-sulfur 1 (4Fe-4S) By similarity HAMAP-Rule MF_00206
Metal binding821Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity HAMAP-Rule MF_00206
Metal binding861Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity HAMAP-Rule MF_00206
Metal binding891Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity HAMAP-Rule MF_00206

Sequences

Sequence LengthMass (Da)Tools
D2QEF5 [UniParc].

Last modified March 2, 2010. Version 1.
Checksum: F74A0C77BC178855

FASTA30234,321
        10         20         30         40         50         60 
MIELPVIPSE QQRNRAGADA APRPKRPDWL RVKLPIGPEY AKVRKLVDEH KLHTICESGN 

        70         80         90        100        110        120 
CPNMGECWGA GTATFMILGN VCTRSCTFCA VATGRPNEYD TDEPRRVAEA IVLMKVKHAV 

       130        140        150        160        170        180 
LTSVNRDELK DRGAEIWYQT VRLIKEASPA TTIETLIPDT KGNWEALERM ISAGQEVVSH 

       190        200        210        220        230        240 
NMETVERLYR RVRPQARYER SLEQIRRTKD YGQRTKSGIM LGLGETKDEV VKAMDDLAAN 

       250        260        270        280        290        300 
GLDILTLGQY LQPTKMHHEV IEWIHPETFA MYREEGLRRG LKYVESGPLV RSSYHAEKHV 


NV 

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Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP001769 Genomic DNA. Translation: ADB36510.1.
RefSeqYP_003385309.1. NC_013730.1.

3D structure databases

ProteinModelPortalD2QEF5.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaADB36510; ADB36510; Slin_0446.
GeneID8724174.
KEGGsli:Slin_0446.
PATRIC32430793. VBISpiLin97822_0453.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMHOG000235997.
KOK03644.
OMAEEYVTPE.

Enzyme and pathway databases

BioCycSLIN504472:GHKB-446-MONOMER.
UniPathwayUPA00538; UER00593.

Family and domain databases

Gene3D3.20.20.70. 1 hit.
HAMAPMF_00206. Lipoyl_synth.
InterProIPR013785. Aldolase_TIM.
IPR006638. Elp3/MiaB/NifB.
IPR003698. Lipoyl_synth.
IPR007197. rSAM.
[Graphical view]
PANTHERPTHR10949. PTHR10949. 1 hit.
PfamPF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFPIRSF005963. Lipoyl_synth. 1 hit.
SMARTSM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsTIGR00510. lipA. 1 hit.
ProtoNetSearch...

Entry information

Entry nameD2QEF5_SPILD
AccessionPrimary (citable) accession number: D2QEF5
Entry history
Integrated into UniProtKB/TrEMBL: March 2, 2010
Last sequence update: March 2, 2010
Last modified: February 19, 2014
This is version 33 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)