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Protein

3-isopropylmalate dehydratase small subunit

Gene

leuD

Organism
Rothia mucilaginosa (strain DY-18) (Stomatococcus mucilaginosus)
Status
Unreviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate.UniRule annotationSAAS annotation

Catalytic activityi

(2R,3S)-3-isopropylmalate = (2S)-2-isopropylmalate.UniRule annotationSAAS annotation

Pathway:iL-leucine biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes L-leucine from 3-methyl-2-oxobutanoate.UniRule annotationSAAS annotation
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. 2-isopropylmalate synthase (leuA)
  2. 3-isopropylmalate dehydratase small subunit (leuD), 3-isopropylmalate dehydratase large subunit (leuC)
  3. no protein annotated in this organism
  4. Branched-chain-amino-acid aminotransferase (RMDY18_07670)
This subpathway is part of the pathway L-leucine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-leucine from 3-methyl-2-oxobutanoate, the pathway L-leucine biosynthesis and in Amino-acid biosynthesis.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

LyaseUniRule annotationSAAS annotation

Keywords - Biological processi

Amino-acid biosynthesis, Branched-chain amino acid biosynthesis, Leucine biosynthesisUniRule annotationSAAS annotation

Enzyme and pathway databases

BioCyciRMUC680646:GH63-796-MONOMER.
UniPathwayiUPA00048; UER00071.

Names & Taxonomyi

Protein namesi
Recommended name:
3-isopropylmalate dehydratase small subunitUniRule annotation (EC:4.2.1.33UniRule annotation)
Alternative name(s):
Alpha-IPM isomeraseUniRule annotation
Isopropylmalate isomeraseUniRule annotation
Gene namesi
Name:leuDUniRule annotation
Ordered Locus Names:RMDY18_08080Imported
OrganismiRothia mucilaginosa (strain DY-18) (Stomatococcus mucilaginosus)Imported
Taxonomic identifieri680646 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaMicrococcalesMicrococcaceaeRothia
ProteomesiUP000001883 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Interactioni

Subunit structurei

Heterodimer of LeuC and LeuD.UniRule annotationSAAS annotation

Protein-protein interaction databases

STRINGi680646.RMDY18_08080.

Family & Domainsi

Sequence similaritiesi

Belongs to the LeuD family. LeuD type 1 subfamily.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000222939.
KOiK01704.
OMAiAFTTHTG.
OrthoDBiEOG661HCP.

Family and domain databases

Gene3Di3.20.19.10. 1 hit.
HAMAPiMF_01031. LeuD_type1.
InterProiIPR004431. 3-IsopropMal_deHydase_ssu.
IPR015937. Acoase/IPM_deHydtase.
IPR015928. Aconitase/3IPM_dehydase_swvl.
IPR000573. AconitaseA/IPMdHydase_ssu_swvl.
[Graphical view]
PANTHERiPTHR11670. PTHR11670. 1 hit.
PfamiPF00694. Aconitase_C. 1 hit.
[Graphical view]
SUPFAMiSSF52016. SSF52016. 1 hit.
TIGRFAMsiTIGR00171. leuD. 1 hit.

Sequencei

Sequence statusi: Complete.

D2NSL4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEPIIKHTGI GVPLRSSNVD TDQIIPAVYL KRVTKTGFDD ALFANWRKDP
60 70 80 90 100
EFILNQEPYK HGSVLVAGPD FGTGSSREHA VWALRDYGFK VVISARFADI
110 120 130 140 150
FRGNSGKQGL VTAQVAQDDI ELIWKALEHE PGTEITVDLE SKTVTVGALV
160 170 180 190
VPFEIDDYVR WRLMEGLDDI GLTLQNLEAI EAYEAKRPSW MPHTQEL
Length:197
Mass (Da):22,079
Last modified:March 2, 2010 - v1
Checksum:iB716420E1EC915DF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP011540 Genomic DNA. Translation: BAI64640.1.
RefSeqiWP_012903330.1. NC_013715.1.

Genome annotation databases

EnsemblBacteriaiBAI64640; BAI64640; RMDY18_08080.
KEGGirmu:RMDY18_08080.
PATRICi35272301. VBIRotMuc152186_0697.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP011540 Genomic DNA. Translation: BAI64640.1.
RefSeqiWP_012903330.1. NC_013715.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi680646.RMDY18_08080.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAI64640; BAI64640; RMDY18_08080.
KEGGirmu:RMDY18_08080.
PATRICi35272301. VBIRotMuc152186_0697.

Phylogenomic databases

HOGENOMiHOG000222939.
KOiK01704.
OMAiAFTTHTG.
OrthoDBiEOG661HCP.

Enzyme and pathway databases

UniPathwayiUPA00048; UER00071.
BioCyciRMUC680646:GH63-796-MONOMER.

Family and domain databases

Gene3Di3.20.19.10. 1 hit.
HAMAPiMF_01031. LeuD_type1.
InterProiIPR004431. 3-IsopropMal_deHydase_ssu.
IPR015937. Acoase/IPM_deHydtase.
IPR015928. Aconitase/3IPM_dehydase_swvl.
IPR000573. AconitaseA/IPMdHydase_ssu_swvl.
[Graphical view]
PANTHERiPTHR11670. PTHR11670. 1 hit.
PfamiPF00694. Aconitase_C. 1 hit.
[Graphical view]
SUPFAMiSSF52016. SSF52016. 1 hit.
TIGRFAMsiTIGR00171. leuD. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Complete genome sequence of Rothia mucilaginosa DJ."
    Yamane K., Nambu T., Mashimo C., Sugimori C., Yamanaka T., Leung K., Fukushima H.
    Submitted (JUL-2009) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: DY-18Imported.

Entry informationi

Entry nameiD2NSL4_ROTMD
AccessioniPrimary (citable) accession number: D2NSL4
Entry historyi
Integrated into UniProtKB/TrEMBL: March 2, 2010
Last sequence update: March 2, 2010
Last modified: July 22, 2015
This is version 42 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteomeImported

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.