D2NRR5 (D2NRR5_ROTMD) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
tRNA pseudouridine synthase A HAMAP-Rule MF_00171
EC=5.4.99.12 HAMAP-Rule MF_00171

Alternative name(s):
tRNA pseudouridine(38-40) synthase HAMAP-Rule MF_00171
tRNA pseudouridylate synthase I HAMAP-Rule MF_00171
tRNA-uridine isomerase I HAMAP-Rule MF_00171

Gene names

Name:	truA HAMAP-Rule MF_00171
Ordered Locus Names:	RMDY18_05090

Organism

Rothia mucilaginosa (strain DY-18) (Stomatococcus mucilaginosus) [Complete proteome] [HAMAP]

Taxonomic identifier

680646 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Actinobacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Micrococcineae › Micrococcaceae › Rothia › Rothia mucilaginosa

Protein attributes

Sequence length	348 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Formation of pseudouridine at positions 38, 39 and 40 in the anticodon stem and loop of transfer RNAs By similarity. HAMAP-Rule MF_00171 SAAS SAAS020094
Catalytic activity	tRNA uridine = tRNA pseudouridine. RuleBase RU003792 tRNA uridine(38-40) = tRNA pseudouridine(38-40). HAMAP-Rule MF_00171 SAAS SAAS020094
Subunit structure	Homodimer By similarity. HAMAP-Rule MF_00171
Sequence similarities	Belongs to the tRNA pseudouridine synthase TruA family. HAMAP-Rule MF_00171 RuleBase RU003792

Ontologies

Keywords
Biological process	tRNA processing HAMAP-Rule MF_00171 SAAS SAAS020094 RuleBase RU003792
Molecular function	Isomerase HAMAP-Rule MF_00171 SAAS SAAS020094 RuleBase RU003792
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	tRNA pseudouridine synthesis Inferred from electronic annotation. Source: HAMAP
Molecular_function	RNA binding Inferred from electronic annotation. Source: InterPro pseudouridine synthase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Sites
	Active site	96	1	Nucleophile By similarity HAMAP-Rule MF_00171
	Binding site	187	1	Substrate By similarity HAMAP-Rule MF_00171

Sequences

	Sequence		Length	Mass (Da)
	D2NRR5 [UniParc]. Last modified March 2, 2010. Version 1. Checksum: AD367ED3C0F33A0D	FASTA	348	38,696
	10 20 30 40 50 60 MELFLRAPSL QEPCQCFRTL SPLKGTPMQN ATALENHEEQ LFIATHRVRI DCAYDGSLFS 70 80 90 100 110 120 GWAAQPNLVT VQGVIENALE LILRAPHRVV VAGRTDAGVH AEAQTLHCDL SDEDWTRLQG 130 140 150 160 170 180 RTGEDDPTAA LGRRLTGALQ RCLYDAEEQL NLPKNVRGIL QGAIQIHRVA EVPFDFDARF 190 200 210 220 230 240 SATGRRYVYR IADGAADGLN PLHRTYTWAV PEHLDCADLN ESAQQLLGLR DFLSFCKPRE 250 260 270 280 290 300 GATTIRELRE LSFTRTESAL IEVCVVADAF CHHMVRSLVG ALVLYGTGKR DAAWLRERIE 310 320 330 340 NPGREASLTL APPHALALAE IYYPAPELYG EQAERARAKR EDDEAQSA

References

[1]	"Complete genome sequence of Rothia mucilaginosa DJ." Yamane K., Nambu T., Mashimo C., Sugimori C., Yamanaka T., Leung K., Fukushima H. Submitted (JUL-2009) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DY-18.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AP011540 Genomic DNA. Translation: BAI64341.1.
RefSeq	YP_003362161.1. NC_013715.1.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	BAI64341; BAI64341; RMDY18_05090.
GeneID	8693518.
KEGG	rmu:RMDY18_05090.
PATRIC	35271763. VBIRotMuc152186_0437.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HOG000248674.
KO	K06173.
OMA	VYLVGAP.
Enzyme and pathway databases
BioCyc	RMUC680646:GH63-548-MONOMER.
Family and domain databases
Gene3D	3.30.70.580. 2 hits. 3.30.70.660. 1 hit.
HAMAP	MF_00171. TruA.
InterPro	IPR020103. PsdUridine_synth_cat_dom. IPR001406. PsdUridine_synth_TruA. IPR020097. PsdUridine_synth_TruA_a/b_dom. IPR020095. PsdUridine_synth_TruA_C. IPR020094. PsdUridine_synth_TruA_N. [Graphical view]
PANTHER	PTHR11142. PTHR11142. 1 hit.
Pfam	PF01416. PseudoU_synth_1. 2 hits. [Graphical view]
SUPFAM	SSF55120. PsdUridine_synth_cat_dom. 1 hit.
TIGRFAMs	TIGR00071. hisT_truA. 1 hit.
ProtoNet	Search...

Entry information

Entry name

D2NRR5_ROTMD

Accession

Primary (citable) accession number: D2NRR5

Entry history

Integrated into UniProtKB/TrEMBL:	March 2, 2010
Last sequence update:	March 2, 2010
Last modified:	May 1, 2013
This is version 26 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)