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D2HHP1

- WEE2_AILME

UniProt

D2HHP1 - WEE2_AILME

Protein

Wee1-like protein kinase 2

Gene

WEE2

Organism
Ailuropoda melanoleuca (Giant panda)
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi
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    • History
      Entry version 34 (01 Oct 2014)
      Sequence version 1 (09 Feb 2010)
      Previous versions | rss
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    Functioni

    Oocyte-specific protein tyrosine kinase that phosphorylates and inhibits CDK1 and acts as a key regulator of meiosis during both prophase I and metaphase II. Required to maintain meiotic arrest in oocytes during the germinal vesicle (GV) stage, a long period of quiescence at dictyate prophase I, by phosphorylating CDK1 at 'Tyr-15', leading to inhibit CDK1 activity and prevent meiotic reentry. Also required for metaphase II exit during egg activation by phosphorylating CDK1 at 'Tyr-15', to ensure exit from meiosis in oocytes and promote pronuclear formation By similarity.By similarity

    Catalytic activityi

    ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.PROSITE-ProRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei243 – 2431ATPPROSITE-ProRule annotation
    Active sitei341 – 3411Proton acceptorPROSITE-ProRule annotation
    Metal bindingi346 – 3461Magnesium; via carbonyl oxygenBy similarity
    Metal bindingi382 – 3821Magnesium; via carbonyl oxygenBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi220 – 2289ATPPROSITE-ProRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. magnesium ion binding Source: InterPro
    3. non-membrane spanning protein tyrosine kinase activity Source: UniProtKB-EC
    4. protein serine/threonine kinase activity Source: InterPro

    GO - Biological processi

    1. female meiotic division Source: UniProtKB
    2. female pronucleus assembly Source: UniProtKB
    3. mitotic nuclear division Source: InterPro
    4. negative regulation of cyclin-dependent protein serine/threonine kinase activity Source: Ensembl
    5. negative regulation of oocyte maturation Source: UniProtKB
    6. regulation of meiosis I Source: UniProtKB

    Keywords - Molecular functioni

    Kinase, Transferase, Tyrosine-protein kinase

    Keywords - Biological processi

    Meiosis

    Keywords - Ligandi

    ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Wee1-like protein kinase 2 (EC:2.7.10.2)
    Alternative name(s):
    Wee1-like protein kinase 1B
    Wee1B kinase
    Gene namesi
    Name:WEE2
    Synonyms:WEE1B
    ORF Names:PANDA_010640
    OrganismiAiluropoda melanoleuca (Giant panda)
    Taxonomic identifieri9646 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCarnivoraCaniformiaUrsidaeAiluropoda
    ProteomesiUP000008912: Unplaced

    Subcellular locationi

    Nucleus By similarity

    GO - Cellular componenti

    1. centrosome Source: Ensembl
    2. cytoplasm Source: UniProtKB
    3. nucleus Source: UniProtKB

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 565565Wee1-like protein kinase 2PRO_0000409524Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei76 – 761PhosphoserineBy similarity

    Post-translational modificationi

    Phosphorylation leads to increase its activity.By similarity

    Keywords - PTMi

    Phosphoprotein

    Structurei

    3D structure databases

    ProteinModelPortaliD2HHP1.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini214 – 492279Protein kinasePROSITE-ProRule annotationAdd
    BLAST

    Coiled coil

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Coiled coili495 – 52127Sequence AnalysisAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi173 – 1753Nuclear localization signalBy similarity
    Motifi317 – 33115Nuclear export signalBy similarityAdd
    BLAST

    Sequence similaritiesi

    Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. WEE1 subfamily.PROSITE-ProRule annotation
    Contains 1 protein kinase domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Coiled coil

    Phylogenomic databases

    GeneTreeiENSGT00530000063230.
    HOGENOMiHOG000004824.
    KOiK06632.
    OMAiICHKMQS.
    OrthoDBiEOG7N63M9.
    TreeFamiTF101088.

    Family and domain databases

    InterProiIPR011009. Kinase-like_dom.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR008271. Ser/Thr_kinase_AS.
    IPR017164. Wee1-like_protein_kinase.
    [Graphical view]
    PfamiPF00069. Pkinase. 1 hit.
    [Graphical view]
    PIRSFiPIRSF037281. Wee1-like_protein_kinase. 1 hit.
    SUPFAMiSSF56112. SSF56112. 1 hit.
    PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    D2HHP1-1 [UniParc]FASTAAdd to Basket

    « Hide

    MDDSSINKEL KQKLNFSYCE EESESEGQEA WETRDAHSQI PDRAEGQESE    50
    AKFTPPGPPL SSVHEVGTFQ EKTKKSPEQV LMTPVSGFRN YPETPAQPDS 100
    RSKLLDCESP FTPKGLLSQS VISSTEKIPS RGSKHLRFTP VPFVDEMTSS 150
    ALVNINPFTP ESYRKQFLKS NGKRKTRGDF EEAGPGEGNV EQGLPAKRCV 200
    LQETNMASRY EKEFLEVEKI GVGEFGTVYK CIKRLDGCVY AIKRSTKPFA 250
    GLSNENLALH EVYAHAVLGH HPHVVRYYSA WAEDDHMIIQ NEYCNGGSLQ 300
    TAISENTKSG NHFQEPKLKD ILLQISLGLK YIHSSGMVHL DIKPSNIFIC 350
    HKMQSDSPVV PEEIENEADW FLSANVMYKI GDLGHVTSIS KPKVEEGDSR 400
    FLANEILQED YQHLPKADIF ALGLTIAVAA GAESLPTNGA AWHHIREGKL 450
    PDIPQKLSEE FYNLLKNMIH PDPRERPSAA ALARSRVLRP SLGKAEELQQ 500
    QLNLEKFKTA TLERELREAQ QAWFSQEERG DAGVSGTPTG SRSTKRLVGG 550
    KSAKSSSFTW GKSSP 565
    Length:565
    Mass (Da):62,913
    Last modified:February 9, 2010 - v1
    Checksum:iE5B24F2A1D99F606
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    GL192847 Genomic DNA. Translation: EFB29558.1.
    RefSeqiXP_002921735.1. XM_002921689.1.

    Genome annotation databases

    EnsembliENSAMET00000016662; ENSAMEP00000015995; ENSAMEG00000015162.
    GeneIDi100468598.
    KEGGiaml:100468598.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    GL192847 Genomic DNA. Translation: EFB29558.1 .
    RefSeqi XP_002921735.1. XM_002921689.1.

    3D structure databases

    ProteinModelPortali D2HHP1.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSAMET00000016662 ; ENSAMEP00000015995 ; ENSAMEG00000015162 .
    GeneIDi 100468598.
    KEGGi aml:100468598.

    Organism-specific databases

    CTDi 494551.

    Phylogenomic databases

    GeneTreei ENSGT00530000063230.
    HOGENOMi HOG000004824.
    KOi K06632.
    OMAi ICHKMQS.
    OrthoDBi EOG7N63M9.
    TreeFami TF101088.

    Family and domain databases

    InterProi IPR011009. Kinase-like_dom.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR008271. Ser/Thr_kinase_AS.
    IPR017164. Wee1-like_protein_kinase.
    [Graphical view ]
    Pfami PF00069. Pkinase. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF037281. Wee1-like_protein_kinase. 1 hit.
    SUPFAMi SSF56112. SSF56112. 1 hit.
    PROSITEi PS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The sequence and de novo assembly of the giant panda genome."
      Li R., Fan W., Tian G., Zhu H., He L., Cai J., Huang Q., Cai Q., Li B., Bai Y., Zhang Z., Zhang Y., Wang W., Li J., Wei F., Li H., Jian M., Li J.
      , Zhang Z., Nielsen R., Li D., Gu W., Yang Z., Xuan Z., Ryder O.A., Leung F.C., Zhou Y., Cao J., Sun X., Fu Y., Fang X., Guo X., Wang B., Hou R., Shen F., Mu B., Ni P., Lin R., Qian W., Wang G., Yu C., Nie W., Wang J., Wu Z., Liang H., Min J., Wu Q., Cheng S., Ruan J., Wang M., Shi Z., Wen M., Liu B., Ren X., Zheng H., Dong D., Cook K., Shan G., Zhang H., Kosiol C., Xie X., Lu Z., Zheng H., Li Y., Steiner C.C., Lam T.T., Lin S., Zhang Q., Li G., Tian J., Gong T., Liu H., Zhang D., Fang L., Ye C., Zhang J., Hu W., Xu A., Ren Y., Zhang G., Bruford M.W., Li Q., Ma L., Guo Y., An N., Hu Y., Zheng Y., Shi Y., Li Z., Liu Q., Chen Y., Zhao J., Qu N., Zhao S., Tian F., Wang X., Wang H., Xu L., Liu X., Vinar T., Wang Y., Lam T.W., Yiu S.M., Liu S., Zhang H., Li D., Huang Y., Wang X., Yang G., Jiang Z., Wang J., Qin N., Li L., Li J., Bolund L., Kristiansen K., Wong G.K., Olson M., Zhang X., Li S., Yang H., Wang J., Wang J.
      Nature 463:311-317(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

    Entry informationi

    Entry nameiWEE2_AILME
    AccessioniPrimary (citable) accession number: D2HHP1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 31, 2011
    Last sequence update: February 9, 2010
    Last modified: October 1, 2014
    This is version 34 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3