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Protein

Wee1-like protein kinase 2

Gene

WEE2

Organism
Ailuropoda melanoleuca (Giant panda)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Oocyte-specific protein tyrosine kinase that phosphorylates and inhibits CDK1 and acts as a key regulator of meiosis during both prophase I and metaphase II. Required to maintain meiotic arrest in oocytes during the germinal vesicle (GV) stage, a long period of quiescence at dictyate prophase I, by phosphorylating CDK1 at 'Tyr-15', leading to inhibit CDK1 activity and prevent meiotic reentry. Also required for metaphase II exit during egg activation by phosphorylating CDK1 at 'Tyr-15', to ensure exit from meiosis in oocytes and promote pronuclear formation (By similarity).By similarity

Catalytic activityi

ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei243ATPPROSITE-ProRule annotation1
Active sitei341Proton acceptorPROSITE-ProRule annotation1
Metal bindingi346Magnesium; via carbonyl oxygenBy similarity1
Metal bindingi382Magnesium; via carbonyl oxygenBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi220 – 228ATPPROSITE-ProRule annotation9

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase, Tyrosine-protein kinase

Keywords - Biological processi

Meiosis

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Wee1-like protein kinase 2 (EC:2.7.10.2)
Alternative name(s):
Wee1-like protein kinase 1B
Wee1B kinase
Gene namesi
Name:WEE2
Synonyms:WEE1B
ORF Names:PANDA_010640
OrganismiAiluropoda melanoleuca (Giant panda)
Taxonomic identifieri9646 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCarnivoraCaniformiaUrsidaeAiluropoda
Proteomesi
  • UP000008912 Componenti: Unassembled WGS sequence

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004095241 – 565Wee1-like protein kinase 2Add BLAST565

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei76PhosphoserineBy similarity1

Post-translational modificationi

Phosphorylation leads to increase its activity.By similarity

Keywords - PTMi

Phosphoprotein

Interactioni

Protein-protein interaction databases

STRINGi9646.ENSAMEP00000015995.

Structurei

3D structure databases

ProteinModelPortaliD2HHP1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini214 – 492Protein kinasePROSITE-ProRule annotationAdd BLAST279

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili495 – 521Sequence analysisAdd BLAST27

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi173 – 175Nuclear localization signalBy similarity3
Motifi317 – 331Nuclear export signalBy similarityAdd BLAST15

Sequence similaritiesi

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. WEE1 subfamily.PROSITE-ProRule annotation
Contains 1 protein kinase domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG0601. Eukaryota.
ENOG410XS1M. LUCA.
GeneTreeiENSGT00530000063230.
HOGENOMiHOG000004824.
InParanoidiD2HHP1.
KOiK06632.
OMAiICHKMQS.
OrthoDBiEOG091G049H.
TreeFamiTF101088.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
IPR017164. Wee1-like_protein_kinase.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
PIRSFiPIRSF037281. Wee1-like_protein_kinase. 1 hit.
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

D2HHP1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDDSSINKEL KQKLNFSYCE EESESEGQEA WETRDAHSQI PDRAEGQESE
60 70 80 90 100
AKFTPPGPPL SSVHEVGTFQ EKTKKSPEQV LMTPVSGFRN YPETPAQPDS
110 120 130 140 150
RSKLLDCESP FTPKGLLSQS VISSTEKIPS RGSKHLRFTP VPFVDEMTSS
160 170 180 190 200
ALVNINPFTP ESYRKQFLKS NGKRKTRGDF EEAGPGEGNV EQGLPAKRCV
210 220 230 240 250
LQETNMASRY EKEFLEVEKI GVGEFGTVYK CIKRLDGCVY AIKRSTKPFA
260 270 280 290 300
GLSNENLALH EVYAHAVLGH HPHVVRYYSA WAEDDHMIIQ NEYCNGGSLQ
310 320 330 340 350
TAISENTKSG NHFQEPKLKD ILLQISLGLK YIHSSGMVHL DIKPSNIFIC
360 370 380 390 400
HKMQSDSPVV PEEIENEADW FLSANVMYKI GDLGHVTSIS KPKVEEGDSR
410 420 430 440 450
FLANEILQED YQHLPKADIF ALGLTIAVAA GAESLPTNGA AWHHIREGKL
460 470 480 490 500
PDIPQKLSEE FYNLLKNMIH PDPRERPSAA ALARSRVLRP SLGKAEELQQ
510 520 530 540 550
QLNLEKFKTA TLERELREAQ QAWFSQEERG DAGVSGTPTG SRSTKRLVGG
560
KSAKSSSFTW GKSSP
Length:565
Mass (Da):62,913
Last modified:February 9, 2010 - v1
Checksum:iE5B24F2A1D99F606
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
GL192847 Genomic DNA. Translation: EFB29558.1.
RefSeqiXP_002921735.1. XM_002921689.2.

Genome annotation databases

EnsembliENSAMET00000016662; ENSAMEP00000015995; ENSAMEG00000015162.
GeneIDi100468598.
KEGGiaml:100468598.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
GL192847 Genomic DNA. Translation: EFB29558.1.
RefSeqiXP_002921735.1. XM_002921689.2.

3D structure databases

ProteinModelPortaliD2HHP1.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9646.ENSAMEP00000015995.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSAMET00000016662; ENSAMEP00000015995; ENSAMEG00000015162.
GeneIDi100468598.
KEGGiaml:100468598.

Organism-specific databases

CTDi494551.

Phylogenomic databases

eggNOGiKOG0601. Eukaryota.
ENOG410XS1M. LUCA.
GeneTreeiENSGT00530000063230.
HOGENOMiHOG000004824.
InParanoidiD2HHP1.
KOiK06632.
OMAiICHKMQS.
OrthoDBiEOG091G049H.
TreeFamiTF101088.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
IPR017164. Wee1-like_protein_kinase.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
PIRSFiPIRSF037281. Wee1-like_protein_kinase. 1 hit.
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiWEE2_AILME
AccessioniPrimary (citable) accession number: D2HHP1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 31, 2011
Last sequence update: February 9, 2010
Last modified: September 7, 2016
This is version 44 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.