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D2HHP1

- WEE2_AILME

UniProt

D2HHP1 - WEE2_AILME

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Protein

Wee1-like protein kinase 2

Gene

WEE2

Organism
Ailuropoda melanoleuca (Giant panda)
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi

Functioni

Oocyte-specific protein tyrosine kinase that phosphorylates and inhibits CDK1 and acts as a key regulator of meiosis during both prophase I and metaphase II. Required to maintain meiotic arrest in oocytes during the germinal vesicle (GV) stage, a long period of quiescence at dictyate prophase I, by phosphorylating CDK1 at 'Tyr-15', leading to inhibit CDK1 activity and prevent meiotic reentry. Also required for metaphase II exit during egg activation by phosphorylating CDK1 at 'Tyr-15', to ensure exit from meiosis in oocytes and promote pronuclear formation (By similarity).By similarity

Catalytic activityi

ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei243 – 2431ATPPROSITE-ProRule annotation
Active sitei341 – 3411Proton acceptorPROSITE-ProRule annotation
Metal bindingi346 – 3461Magnesium; via carbonyl oxygenBy similarity
Metal bindingi382 – 3821Magnesium; via carbonyl oxygenBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi220 – 2289ATPPROSITE-ProRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. magnesium ion binding Source: InterPro
  3. non-membrane spanning protein tyrosine kinase activity Source: UniProtKB-EC
  4. protein serine/threonine kinase activity Source: InterPro

GO - Biological processi

  1. female meiotic division Source: UniProtKB
  2. female pronucleus assembly Source: UniProtKB
  3. mitotic nuclear division Source: InterPro
  4. negative regulation of cyclin-dependent protein serine/threonine kinase activity Source: Ensembl
  5. negative regulation of oocyte maturation Source: UniProtKB
  6. regulation of meiosis I Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase, Tyrosine-protein kinase

Keywords - Biological processi

Meiosis

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Wee1-like protein kinase 2 (EC:2.7.10.2)
Alternative name(s):
Wee1-like protein kinase 1B
Wee1B kinase
Gene namesi
Name:WEE2
Synonyms:WEE1B
ORF Names:PANDA_010640
OrganismiAiluropoda melanoleuca (Giant panda)
Taxonomic identifieri9646 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCarnivoraCaniformiaUrsidaeAiluropoda
ProteomesiUP000008912: Unplaced

Subcellular locationi

Nucleus By similarity

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB
  2. nucleus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 565565Wee1-like protein kinase 2PRO_0000409524Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei76 – 761PhosphoserineBy similarity

Post-translational modificationi

Phosphorylation leads to increase its activity.By similarity

Keywords - PTMi

Phosphoprotein

Structurei

3D structure databases

ProteinModelPortaliD2HHP1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini214 – 492279Protein kinasePROSITE-ProRule annotationAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili495 – 52127Sequence AnalysisAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi173 – 1753Nuclear localization signalBy similarity
Motifi317 – 33115Nuclear export signalBy similarityAdd
BLAST

Sequence similaritiesi

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. WEE1 subfamily.PROSITE-ProRule annotation
Contains 1 protein kinase domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil

Phylogenomic databases

GeneTreeiENSGT00530000063230.
HOGENOMiHOG000004824.
InParanoidiD2HHP1.
KOiK06632.
OMAiICHKMQS.
OrthoDBiEOG7N63M9.
TreeFamiTF101088.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
IPR017164. Wee1-like_protein_kinase.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
PIRSFiPIRSF037281. Wee1-like_protein_kinase. 1 hit.
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

D2HHP1-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MDDSSINKEL KQKLNFSYCE EESESEGQEA WETRDAHSQI PDRAEGQESE
60 70 80 90 100
AKFTPPGPPL SSVHEVGTFQ EKTKKSPEQV LMTPVSGFRN YPETPAQPDS
110 120 130 140 150
RSKLLDCESP FTPKGLLSQS VISSTEKIPS RGSKHLRFTP VPFVDEMTSS
160 170 180 190 200
ALVNINPFTP ESYRKQFLKS NGKRKTRGDF EEAGPGEGNV EQGLPAKRCV
210 220 230 240 250
LQETNMASRY EKEFLEVEKI GVGEFGTVYK CIKRLDGCVY AIKRSTKPFA
260 270 280 290 300
GLSNENLALH EVYAHAVLGH HPHVVRYYSA WAEDDHMIIQ NEYCNGGSLQ
310 320 330 340 350
TAISENTKSG NHFQEPKLKD ILLQISLGLK YIHSSGMVHL DIKPSNIFIC
360 370 380 390 400
HKMQSDSPVV PEEIENEADW FLSANVMYKI GDLGHVTSIS KPKVEEGDSR
410 420 430 440 450
FLANEILQED YQHLPKADIF ALGLTIAVAA GAESLPTNGA AWHHIREGKL
460 470 480 490 500
PDIPQKLSEE FYNLLKNMIH PDPRERPSAA ALARSRVLRP SLGKAEELQQ
510 520 530 540 550
QLNLEKFKTA TLERELREAQ QAWFSQEERG DAGVSGTPTG SRSTKRLVGG
560
KSAKSSSFTW GKSSP
Length:565
Mass (Da):62,913
Last modified:February 9, 2010 - v1
Checksum:iE5B24F2A1D99F606
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
GL192847 Genomic DNA. Translation: EFB29558.1.
RefSeqiXP_002921735.1. XM_002921689.1.

Genome annotation databases

EnsembliENSAMET00000016662; ENSAMEP00000015995; ENSAMEG00000015162.
GeneIDi100468598.
KEGGiaml:100468598.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
GL192847 Genomic DNA. Translation: EFB29558.1 .
RefSeqi XP_002921735.1. XM_002921689.1.

3D structure databases

ProteinModelPortali D2HHP1.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSAMET00000016662 ; ENSAMEP00000015995 ; ENSAMEG00000015162 .
GeneIDi 100468598.
KEGGi aml:100468598.

Organism-specific databases

CTDi 494551.

Phylogenomic databases

GeneTreei ENSGT00530000063230.
HOGENOMi HOG000004824.
InParanoidi D2HHP1.
KOi K06632.
OMAi ICHKMQS.
OrthoDBi EOG7N63M9.
TreeFami TF101088.

Family and domain databases

InterProi IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
IPR017164. Wee1-like_protein_kinase.
[Graphical view ]
Pfami PF00069. Pkinase. 1 hit.
[Graphical view ]
PIRSFi PIRSF037281. Wee1-like_protein_kinase. 1 hit.
SUPFAMi SSF56112. SSF56112. 1 hit.
PROSITEi PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "The sequence and de novo assembly of the giant panda genome."
    Li R., Fan W., Tian G., Zhu H., He L., Cai J., Huang Q., Cai Q., Li B., Bai Y., Zhang Z., Zhang Y., Wang W., Li J., Wei F., Li H., Jian M., Li J.
    , Zhang Z., Nielsen R., Li D., Gu W., Yang Z., Xuan Z., Ryder O.A., Leung F.C., Zhou Y., Cao J., Sun X., Fu Y., Fang X., Guo X., Wang B., Hou R., Shen F., Mu B., Ni P., Lin R., Qian W., Wang G., Yu C., Nie W., Wang J., Wu Z., Liang H., Min J., Wu Q., Cheng S., Ruan J., Wang M., Shi Z., Wen M., Liu B., Ren X., Zheng H., Dong D., Cook K., Shan G., Zhang H., Kosiol C., Xie X., Lu Z., Zheng H., Li Y., Steiner C.C., Lam T.T., Lin S., Zhang Q., Li G., Tian J., Gong T., Liu H., Zhang D., Fang L., Ye C., Zhang J., Hu W., Xu A., Ren Y., Zhang G., Bruford M.W., Li Q., Ma L., Guo Y., An N., Hu Y., Zheng Y., Shi Y., Li Z., Liu Q., Chen Y., Zhao J., Qu N., Zhao S., Tian F., Wang X., Wang H., Xu L., Liu X., Vinar T., Wang Y., Lam T.W., Yiu S.M., Liu S., Zhang H., Li D., Huang Y., Wang X., Yang G., Jiang Z., Wang J., Qin N., Li L., Li J., Bolund L., Kristiansen K., Wong G.K., Olson M., Zhang X., Li S., Yang H., Wang J., Wang J.
    Nature 463:311-317(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Entry informationi

Entry nameiWEE2_AILME
AccessioniPrimary (citable) accession number: D2HHP1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 31, 2011
Last sequence update: February 9, 2010
Last modified: October 29, 2014
This is version 35 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3