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D2HHP1

- WEE2_AILME

UniProt

D2HHP1 - WEE2_AILME

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Protein
Wee1-like protein kinase 2
Gene
WEE2, WEE1B, PANDA_010640
Organism
Ailuropoda melanoleuca (Giant panda)
Status
Reviewed - Annotation score: 4 out of 5 - Protein inferred from homologyi

Functioni

Oocyte-specific protein tyrosine kinase that phosphorylates and inhibits CDK1 and acts as a key regulator of meiosis during both prophase I and metaphase II. Required to maintain meiotic arrest in oocytes during the germinal vesicle (GV) stage, a long period of quiescence at dictyate prophase I, by phosphorylating CDK1 at 'Tyr-15', leading to inhibit CDK1 activity and prevent meiotic reentry. Also required for metaphase II exit during egg activation by phosphorylating CDK1 at 'Tyr-15', to ensure exit from meiosis in oocytes and promote pronuclear formation By similarity.

Catalytic activityi

ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei243 – 2431ATP By similarity
Active sitei341 – 3411Proton acceptor By similarity
Metal bindingi346 – 3461Magnesium; via carbonyl oxygen By similarity
Metal bindingi382 – 3821Magnesium; via carbonyl oxygen By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi220 – 2289ATP By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. magnesium ion binding Source: InterPro
  3. non-membrane spanning protein tyrosine kinase activity Source: UniProtKB-EC
  4. protein serine/threonine kinase activity Source: InterPro

GO - Biological processi

  1. female meiotic division Source: UniProtKB
  2. female pronucleus assembly Source: UniProtKB
  3. mitotic nuclear division Source: InterPro
  4. negative regulation of cyclin-dependent protein serine/threonine kinase activity Source: Ensembl
  5. negative regulation of oocyte maturation Source: UniProtKB
  6. regulation of meiosis I Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase, Tyrosine-protein kinase

Keywords - Biological processi

Meiosis

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Wee1-like protein kinase 2 (EC:2.7.10.2)
Alternative name(s):
Wee1-like protein kinase 1B
Wee1B kinase
Gene namesi
Name:WEE2
Synonyms:WEE1B
ORF Names:PANDA_010640
OrganismiAiluropoda melanoleuca (Giant panda)
Taxonomic identifieri9646 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCarnivoraCaniformiaUrsidaeAiluropoda
ProteomesiUP000008912: Unplaced

Subcellular locationi

Nucleus By similarity

GO - Cellular componenti

  1. centrosome Source: Ensembl
  2. cytoplasm Source: UniProtKB
  3. nucleus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 565565Wee1-like protein kinase 2
PRO_0000409524Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei76 – 761Phosphoserine By similarity

Post-translational modificationi

Phosphorylation leads to increase its activity By similarity.

Keywords - PTMi

Phosphoprotein

Structurei

3D structure databases

ProteinModelPortaliD2HHP1.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini214 – 492279Protein kinase
Add
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili495 – 52127 Reviewed prediction
Add
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi173 – 1753Nuclear localization signal By similarity
Motifi317 – 33115Nuclear export signal By similarity
Add
BLAST

Sequence similaritiesi

Keywords - Domaini

Coiled coil

Phylogenomic databases

GeneTreeiENSGT00530000063230.
HOGENOMiHOG000004824.
KOiK06632.
OMAiICHKMQS.
OrthoDBiEOG7N63M9.
TreeFamiTF101088.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
IPR017164. Wee1-like_protein_kinase.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
PIRSFiPIRSF037281. Wee1-like_protein_kinase. 1 hit.
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

D2HHP1-1 [UniParc]FASTAAdd to Basket

« Hide

MDDSSINKEL KQKLNFSYCE EESESEGQEA WETRDAHSQI PDRAEGQESE    50
AKFTPPGPPL SSVHEVGTFQ EKTKKSPEQV LMTPVSGFRN YPETPAQPDS 100
RSKLLDCESP FTPKGLLSQS VISSTEKIPS RGSKHLRFTP VPFVDEMTSS 150
ALVNINPFTP ESYRKQFLKS NGKRKTRGDF EEAGPGEGNV EQGLPAKRCV 200
LQETNMASRY EKEFLEVEKI GVGEFGTVYK CIKRLDGCVY AIKRSTKPFA 250
GLSNENLALH EVYAHAVLGH HPHVVRYYSA WAEDDHMIIQ NEYCNGGSLQ 300
TAISENTKSG NHFQEPKLKD ILLQISLGLK YIHSSGMVHL DIKPSNIFIC 350
HKMQSDSPVV PEEIENEADW FLSANVMYKI GDLGHVTSIS KPKVEEGDSR 400
FLANEILQED YQHLPKADIF ALGLTIAVAA GAESLPTNGA AWHHIREGKL 450
PDIPQKLSEE FYNLLKNMIH PDPRERPSAA ALARSRVLRP SLGKAEELQQ 500
QLNLEKFKTA TLERELREAQ QAWFSQEERG DAGVSGTPTG SRSTKRLVGG 550
KSAKSSSFTW GKSSP 565
Length:565
Mass (Da):62,913
Last modified:February 9, 2010 - v1
Checksum:iE5B24F2A1D99F606
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
GL192847 Genomic DNA. Translation: EFB29558.1.
RefSeqiXP_002921735.1. XM_002921689.1.

Genome annotation databases

EnsembliENSAMET00000016662; ENSAMEP00000015995; ENSAMEG00000015162.
GeneIDi100468598.
KEGGiaml:100468598.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
GL192847 Genomic DNA. Translation: EFB29558.1 .
RefSeqi XP_002921735.1. XM_002921689.1.

3D structure databases

ProteinModelPortali D2HHP1.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSAMET00000016662 ; ENSAMEP00000015995 ; ENSAMEG00000015162 .
GeneIDi 100468598.
KEGGi aml:100468598.

Organism-specific databases

CTDi 494551.

Phylogenomic databases

GeneTreei ENSGT00530000063230.
HOGENOMi HOG000004824.
KOi K06632.
OMAi ICHKMQS.
OrthoDBi EOG7N63M9.
TreeFami TF101088.

Family and domain databases

InterProi IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
IPR017164. Wee1-like_protein_kinase.
[Graphical view ]
Pfami PF00069. Pkinase. 1 hit.
[Graphical view ]
PIRSFi PIRSF037281. Wee1-like_protein_kinase. 1 hit.
SUPFAMi SSF56112. SSF56112. 1 hit.
PROSITEi PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "The sequence and de novo assembly of the giant panda genome."
    Li R., Fan W., Tian G., Zhu H., He L., Cai J., Huang Q., Cai Q., Li B., Bai Y., Zhang Z., Zhang Y., Wang W., Li J., Wei F., Li H., Jian M., Li J.
    , Zhang Z., Nielsen R., Li D., Gu W., Yang Z., Xuan Z., Ryder O.A., Leung F.C., Zhou Y., Cao J., Sun X., Fu Y., Fang X., Guo X., Wang B., Hou R., Shen F., Mu B., Ni P., Lin R., Qian W., Wang G., Yu C., Nie W., Wang J., Wu Z., Liang H., Min J., Wu Q., Cheng S., Ruan J., Wang M., Shi Z., Wen M., Liu B., Ren X., Zheng H., Dong D., Cook K., Shan G., Zhang H., Kosiol C., Xie X., Lu Z., Zheng H., Li Y., Steiner C.C., Lam T.T., Lin S., Zhang Q., Li G., Tian J., Gong T., Liu H., Zhang D., Fang L., Ye C., Zhang J., Hu W., Xu A., Ren Y., Zhang G., Bruford M.W., Li Q., Ma L., Guo Y., An N., Hu Y., Zheng Y., Shi Y., Li Z., Liu Q., Chen Y., Zhao J., Qu N., Zhao S., Tian F., Wang X., Wang H., Xu L., Liu X., Vinar T., Wang Y., Lam T.W., Yiu S.M., Liu S., Zhang H., Li D., Huang Y., Wang X., Yang G., Jiang Z., Wang J., Qin N., Li L., Li J., Bolund L., Kristiansen K., Wong G.K., Olson M., Zhang X., Li S., Yang H., Wang J., Wang J.
    Nature 463:311-317(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Entry informationi

Entry nameiWEE2_AILME
AccessioniPrimary (citable) accession number: D2HHP1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 31, 2011
Last sequence update: February 9, 2010
Last modified: September 3, 2014
This is version 33 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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