D2HHP1 (WEE2_AILME) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Wee1-like protein kinase 2
EC=2.7.10.2

Alternative name(s):
Wee1-like protein kinase 1B
Wee1B kinase

Gene names

Name:	WEE2
Synonyms:	WEE1B
ORF Names:	PANDA_010640

Organism

Ailuropoda melanoleuca (Giant panda) [Reference proteome]

Taxonomic identifier

9646 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Laurasiatheria › Carnivora › Caniformia › Ursidae › Ailuropoda

Protein attributes

Sequence length	565 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Oocyte-specific protein tyrosine kinase that phosphorylates and inhibits CDK1 and acts as a key regulator of meiosis during both prophase I and metaphase II. Required to maintain meiotic arrest in oocytes during the germinal vesicle (GV) stage, a long period of quiescence at dictyate prophase I, by phosphorylating CDK1 at 'Tyr-15', leading to inhibit CDK1 activity and prevent meiotic reentry. Also required for metaphase II exit during egg activation by phosphorylating CDK1 at 'Tyr-15', to ensure exit from meiosis in oocytes and promote pronuclear formation By similarity.
Catalytic activity	ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.
Subcellular location	Nucleus By similarity.
Post-translational modification	Phosphorylation leads to increase its activity By similarity.
Sequence similarities	Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. WEE1 subfamily. Contains 1 protein kinase domain.

Ontologies

Keywords
Biological process	Meiosis
Cellular component	Nucleus
Domain	Coiled coil
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Kinase Transferase Tyrosine-protein kinase
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	egg activation Inferred from sequence or structural similarity. Source: UniProtKB female meiosis Inferred from sequence or structural similarity. Source: UniProtKB female pronucleus assembly Inferred from sequence or structural similarity. Source: UniProtKB meiotic metaphase II Inferred from sequence or structural similarity. Source: UniProtKB meiotic prophase I Inferred from sequence or structural similarity. Source: UniProtKB mitosis Inferred from electronic annotation. Source: InterPro negative regulation of oocyte development Inferred from sequence or structural similarity. Source: UniProtKB regulation of meiosis I Inferred from sequence or structural similarity. Source: UniProtKB
Cellular_component	centrosome Inferred from electronic annotation. Source: Compara cytoplasm Inferred from sequence or structural similarity. Source: UniProtKB nucleus Inferred from sequence or structural similarity. Source: UniProtKB
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW magnesium ion binding Inferred from electronic annotation. Source: InterPro non-membrane spanning protein tyrosine kinase activity Inferred from electronic annotation. Source: EC protein serine/threonine kinase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 565	565	Wee1-like protein kinase 2		PRO_0000409524
Regions
	Domain	214 – 492	279	Protein kinase
	Nucleotide binding	220 – 228	9	ATP By similarity
	Coiled coil	495 – 521	27	Potential
	Motif	173 – 175	3	Nuclear localization signal By similarity
	Motif	317 – 331	15	Nuclear export signal By similarity
Sites
	Active site	341	1	Proton acceptor By similarity
	Metal binding	346	1	Magnesium; via carbonyl oxygen By similarity
	Metal binding	382	1	Magnesium; via carbonyl oxygen By similarity
	Binding site	243	1	ATP By similarity
Amino acid modifications
	Modified residue	76	1	Phosphoserine By similarity

Sequences

	Sequence		Length	Mass (Da)
	D2HHP1 [UniParc]. Last modified February 9, 2010. Version 1. Checksum: E5B24F2A1D99F606	FASTA	565	62,913
	10 20 30 40 50 60 MDDSSINKEL KQKLNFSYCE EESESEGQEA WETRDAHSQI PDRAEGQESE AKFTPPGPPL 70 80 90 100 110 120 SSVHEVGTFQ EKTKKSPEQV LMTPVSGFRN YPETPAQPDS RSKLLDCESP FTPKGLLSQS 130 140 150 160 170 180 VISSTEKIPS RGSKHLRFTP VPFVDEMTSS ALVNINPFTP ESYRKQFLKS NGKRKTRGDF 190 200 210 220 230 240 EEAGPGEGNV EQGLPAKRCV LQETNMASRY EKEFLEVEKI GVGEFGTVYK CIKRLDGCVY 250 260 270 280 290 300 AIKRSTKPFA GLSNENLALH EVYAHAVLGH HPHVVRYYSA WAEDDHMIIQ NEYCNGGSLQ 310 320 330 340 350 360 TAISENTKSG NHFQEPKLKD ILLQISLGLK YIHSSGMVHL DIKPSNIFIC HKMQSDSPVV 370 380 390 400 410 420 PEEIENEADW FLSANVMYKI GDLGHVTSIS KPKVEEGDSR FLANEILQED YQHLPKADIF 430 440 450 460 470 480 ALGLTIAVAA GAESLPTNGA AWHHIREGKL PDIPQKLSEE FYNLLKNMIH PDPRERPSAA 490 500 510 520 530 540 ALARSRVLRP SLGKAEELQQ QLNLEKFKTA TLERELREAQ QAWFSQEERG DAGVSGTPTG 550 560 SRSTKRLVGG KSAKSSSFTW GKSSP

References

[1]

"The sequence and de novo assembly of the giant panda genome." Li R., Fan W., Tian G., Zhu H., He L., Cai J., Huang Q., Cai Q., Li B., Bai Y., Zhang Z., Zhang Y., Wang W., Li J., Wei F., Li H., Jian M., Li J. , Zhang Z., Nielsen R., Li D., Gu W., Yang Z., Xuan Z., Ryder O.A., Leung F.C., Zhou Y., Cao J., Sun X., Fu Y., Fang X., Guo X., Wang B., Hou R., Shen F., Mu B., Ni P., Lin R., Qian W., Wang G., Yu C., Nie W., Wang J., Wu Z., Liang H., Min J., Wu Q., Cheng S., Ruan J., Wang M., Shi Z., Wen M., Liu B., Ren X., Zheng H., Dong D., Cook K., Shan G., Zhang H., Kosiol C., Xie X., Lu Z., Zheng H., Li Y., Steiner C.C., Lam T.T., Lin S., Zhang Q., Li G., Tian J., Gong T., Liu H., Zhang D., Fang L., Ye C., Zhang J., Hu W., Xu A., Ren Y., Zhang G., Bruford M.W., Li Q., Ma L., Guo Y., An N., Hu Y., Zheng Y., Shi Y., Li Z., Liu Q., Chen Y., Zhao J., Qu N., Zhao S., Tian F., Wang X., Wang H., Xu L., Liu X., Vinar T., Wang Y., Lam T.W., Yiu S.M., Liu S., Zhang H., Li D., Huang Y., Wang X., Yang G., Jiang Z., Wang J., Qin N., Li L., Li J., Bolund L., Kristiansen K., Wong G.K., Olson M., Zhang X., Li S., Yang H., Wang J., Wang J. Nature 463:311-317(2010) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	GL192847 Genomic DNA. Translation: EFB29558.1.
RefSeq	XP_002921735.1. XM_002921689.1.
3D structure databases
ProteinModelPortal	D2HHP1.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSAMET00000016662; ENSAMEP00000015995; ENSAMEG00000015162.
GeneID	100468598.
KEGG	aml:100468598.
Organism-specific databases
CTD	494551.
Phylogenomic databases
GeneTree	ENSGT00530000063230.
HOGENOM	HOG000004824.
KO	K06632.
OMA	ANVMYKI.
Family and domain databases
InterPro	IPR011009. Kinase-like_dom. IPR000719. Prot_kinase_cat_dom. IPR017441. Protein_kinase_ATP_BS. IPR008271. Ser/Thr_kinase_AS. IPR017164. Wee1-like_protein_kinase. [Graphical view]
Pfam	PF00069. Pkinase. 1 hit. [Graphical view]
PIRSF	PIRSF037281. Wee1-like_protein_kinase. 1 hit.
SUPFAM	SSF56112. Kinase_like. 1 hit.
PROSITE	PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

WEE2_AILME

Accession

Primary (citable) accession number: D2HHP1

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 31, 2011
Last sequence update:	February 9, 2010
Last modified:	March 6, 2013
This is version 23 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program