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D2HBV9

- PORED_AILME

UniProt

D2HBV9 - PORED_AILME

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Protein

Polyprenol reductase

Gene

SRD5A3

Organism
Ailuropoda melanoleuca (Giant panda)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Plays a key role in early steps of protein N-linked glycosylation by being required for the conversion of polyprenol into dolichol. Dolichols are required for the synthesis of dolichol-linked monosaccharides and the oligosaccharide precursor used for N-glycosylation. Acts as a polyprenol reductase that promotes the reduction of the alpha-isoprene unit of polyprenols into dolichols in a NADP-dependent mechanism. Also able to convert testosterone (T) into 5-alpha-dihydrotestosterone (DHT) (By similarity).By similarity

Catalytic activityi

Ditrans,polycis-dolichol + NADP+ = ditrans,polycis-polyprenol + NADPH.
A 3-oxo-5-alpha-steroid + NADP+ = a 3-oxo-Delta(4)-steroid + NADPH.

Pathwayi

GO - Molecular functioni

  1. 3-oxo-5-alpha-steroid 4-dehydrogenase activity Source: UniProtKB
  2. cholestenone 5-alpha-reductase activity Source: UniProtKB-EC
  3. oxidoreductase activity, acting on the CH-CH group of donors, NAD or NADP as acceptor Source: UniProtKB

GO - Biological processi

  1. dolichol-linked oligosaccharide biosynthetic process Source: UniProtKB
  2. dolichol metabolic process Source: UniProtKB
  3. polyprenol catabolic process Source: UniProtKB
  4. protein glycosylation Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

NADP

Enzyme and pathway databases

UniPathwayiUPA00378.

Names & Taxonomyi

Protein namesi
Recommended name:
Polyprenol reductase (EC:1.3.1.94)
Alternative name(s):
3-oxo-5-alpha-steroid 4-dehydrogenase 3 (EC:1.3.1.22)
Steroid 5-alpha-reductase 3
Short name:
S5AR 3
Short name:
SR type 3
Gene namesi
Name:SRD5A3
ORF Names:PANDA_008038
OrganismiAiluropoda melanoleuca (Giant panda)
Taxonomic identifieri9646 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCarnivoraCaniformiaUrsidaeAiluropoda
ProteomesiUP000008912: Unplaced

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 1919CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei20 – 4021HelicalSequence AnalysisAdd
BLAST
Topological domaini41 – 8040LumenalSequence AnalysisAdd
BLAST
Transmembranei81 – 10121HelicalSequence AnalysisAdd
BLAST
Topological domaini102 – 11918CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei120 – 14021HelicalSequence AnalysisAdd
BLAST
Topological domaini141 – 15616LumenalSequence AnalysisAdd
BLAST
Transmembranei157 – 17721HelicalSequence AnalysisAdd
BLAST
Topological domaini178 – 19417CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei195 – 21521HelicalSequence AnalysisAdd
BLAST
Topological domaini216 – 26550LumenalSequence AnalysisAdd
BLAST
Transmembranei266 – 28621HelicalSequence AnalysisAdd
BLAST
Topological domaini287 – 31832CytoplasmicSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. endoplasmic reticulum Source: UniProtKB
  2. integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 318318Polyprenol reductasePRO_0000398648Add
BLAST

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

HOGENOMiHOG000018885.
InParanoidiD2HBV9.
KOiK12345.

Family and domain databases

InterProiIPR001104. 3-oxo-5_a-steroid_4-DH_C.
[Graphical view]
PfamiPF02544. Steroid_dh. 1 hit.
[Graphical view]
PROSITEiPS50244. S5A_REDUCTASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

D2HBV9-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MAPWAAAQLW ALNPLRALWL TLAAAFLLTL LLQLVPPGLL PGCALFQDLI
60 70 80 90 100
RYGKTKREGQ SRPAVCRVFD VPKRYFSHFY IISALWNGFL LWHLTQSVFL
110 120 130 140 150
GVPFPNWLHG LLRILGASQF QGGELALSAF LVLVFLWLHS LRRLFECFYV
160 170 180 190 200
SVFSNTVIHI VQYCFGLVYY VLTGLTVLSQ VPMDGRNAYV IGKNLLMQAR
210 220 230 240 250
WFHILGMLMF IWSSVHQYKC HVILGNLRKN KAGVVIHCNH RIPFGDWFEY
260 270 280 290 300
VSSPNYLAEL MIYISMAVTF GFHNLTWWLV VTYVFFSQAL SAFLSHKFYK
310
SKFVSYPKHR KAFLPFLF
Length:318
Mass (Da):36,775
Last modified:February 9, 2010 - v1
Checksum:iB0CD88A5D8E2667D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
GL192668 Genomic DNA. Translation: EFB15787.1.
RefSeqiXP_002919412.1. XM_002919366.1.

Genome annotation databases

GeneIDi100463734.
KEGGiaml:100463734.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
GL192668 Genomic DNA. Translation: EFB15787.1 .
RefSeqi XP_002919412.1. XM_002919366.1.

3D structure databases

ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 100463734.
KEGGi aml:100463734.

Organism-specific databases

CTDi 79644.

Phylogenomic databases

HOGENOMi HOG000018885.
InParanoidi D2HBV9.
KOi K12345.

Enzyme and pathway databases

UniPathwayi UPA00378 .

Family and domain databases

InterProi IPR001104. 3-oxo-5_a-steroid_4-DH_C.
[Graphical view ]
Pfami PF02544. Steroid_dh. 1 hit.
[Graphical view ]
PROSITEi PS50244. S5A_REDUCTASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "The sequence and de novo assembly of the giant panda genome."
    Li R., Fan W., Tian G., Zhu H., He L., Cai J., Huang Q., Cai Q., Li B., Bai Y., Zhang Z., Zhang Y., Wang W., Li J., Wei F., Li H., Jian M., Li J.
    , Zhang Z., Nielsen R., Li D., Gu W., Yang Z., Xuan Z., Ryder O.A., Leung F.C., Zhou Y., Cao J., Sun X., Fu Y., Fang X., Guo X., Wang B., Hou R., Shen F., Mu B., Ni P., Lin R., Qian W., Wang G., Yu C., Nie W., Wang J., Wu Z., Liang H., Min J., Wu Q., Cheng S., Ruan J., Wang M., Shi Z., Wen M., Liu B., Ren X., Zheng H., Dong D., Cook K., Shan G., Zhang H., Kosiol C., Xie X., Lu Z., Zheng H., Li Y., Steiner C.C., Lam T.T., Lin S., Zhang Q., Li G., Tian J., Gong T., Liu H., Zhang D., Fang L., Ye C., Zhang J., Hu W., Xu A., Ren Y., Zhang G., Bruford M.W., Li Q., Ma L., Guo Y., An N., Hu Y., Zheng Y., Shi Y., Li Z., Liu Q., Chen Y., Zhao J., Qu N., Zhao S., Tian F., Wang X., Wang H., Xu L., Liu X., Vinar T., Wang Y., Lam T.W., Yiu S.M., Liu S., Zhang H., Li D., Huang Y., Wang X., Yang G., Jiang Z., Wang J., Qin N., Li L., Li J., Bolund L., Kristiansen K., Wong G.K., Olson M., Zhang X., Li S., Yang H., Wang J., Wang J.
    Nature 463:311-317(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Entry informationi

Entry nameiPORED_AILME
AccessioniPrimary (citable) accession number: D2HBV9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 5, 2010
Last sequence update: February 9, 2010
Last modified: October 29, 2014
This is version 25 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3