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D2HBV9 (PORED_AILME) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 23. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Polyprenol reductase

EC=1.3.1.94
Alternative name(s):
3-oxo-5-alpha-steroid 4-dehydrogenase 3
EC=1.3.1.22
Steroid 5-alpha-reductase 3
Short name=S5AR 3
Short name=SR type 3
Gene names
Name:SRD5A3
ORF Names:PANDA_008038
OrganismAiluropoda melanoleuca (Giant panda) [Reference proteome]
Taxonomic identifier9646 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCarnivoraCaniformiaUrsidaeAiluropoda

Protein attributes

Sequence length318 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Plays a key role in early steps of protein N-linked glycosylation by being required for the conversion of polyprenol into dolichol. Dolichols are required for the synthesis of dolichol-linked monosaccharides and the oligosaccharide precursor used for N-glycosylation. Acts as a polyprenol reductase that promotes the reduction of the alpha-isoprene unit of polyprenols into dolichols in a NADP-dependent mechanism. Also able to convert testosterone (T) into 5-alpha-dihydrotestosterone (DHT) By similarity.

Catalytic activity

Ditrans,polycis-dolichol + NADP+ = ditrans,polycis-polyprenol + NADPH.

A 3-oxo-5-alpha-steroid + NADP+ = a 3-oxo-Delta(4)-steroid + NADPH.

Pathway

Protein modification; protein glycosylation.

Subcellular location

Endoplasmic reticulum membrane; Multi-pass membrane protein By similarity.

Sequence similarities

Belongs to the steroid 5-alpha reductase family. Polyprenol reductase subfamily.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 318318Polyprenol reductase
PRO_0000398648

Regions

Topological domain1 – 1919Cytoplasmic Potential
Transmembrane20 – 4021Helical; Potential
Topological domain41 – 8040Lumenal Potential
Transmembrane81 – 10121Helical; Potential
Topological domain102 – 11918Cytoplasmic Potential
Transmembrane120 – 14021Helical; Potential
Topological domain141 – 15616Lumenal Potential
Transmembrane157 – 17721Helical; Potential
Topological domain178 – 19417Cytoplasmic Potential
Transmembrane195 – 21521Helical; Potential
Topological domain216 – 26550Lumenal Potential
Transmembrane266 – 28621Helical; Potential
Topological domain287 – 31832Cytoplasmic Potential

Sequences

Sequence LengthMass (Da)Tools
D2HBV9 [UniParc].

Last modified February 9, 2010. Version 1.
Checksum: B0CD88A5D8E2667D

FASTA31836,775
        10         20         30         40         50         60 
MAPWAAAQLW ALNPLRALWL TLAAAFLLTL LLQLVPPGLL PGCALFQDLI RYGKTKREGQ 

        70         80         90        100        110        120 
SRPAVCRVFD VPKRYFSHFY IISALWNGFL LWHLTQSVFL GVPFPNWLHG LLRILGASQF 

       130        140        150        160        170        180 
QGGELALSAF LVLVFLWLHS LRRLFECFYV SVFSNTVIHI VQYCFGLVYY VLTGLTVLSQ 

       190        200        210        220        230        240 
VPMDGRNAYV IGKNLLMQAR WFHILGMLMF IWSSVHQYKC HVILGNLRKN KAGVVIHCNH 

       250        260        270        280        290        300 
RIPFGDWFEY VSSPNYLAEL MIYISMAVTF GFHNLTWWLV VTYVFFSQAL SAFLSHKFYK 

       310 
SKFVSYPKHR KAFLPFLF 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
GL192668 Genomic DNA. Translation: EFB15787.1.
RefSeqXP_002919412.1. XM_002919366.1.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID100463734.
KEGGaml:100463734.

Organism-specific databases

CTD79644.

Phylogenomic databases

HOGENOMHOG000018885.
KOK12345.

Enzyme and pathway databases

UniPathwayUPA00378.

Family and domain databases

InterProIPR001104. 3-oxo-5_a-steroid_4-DH_C.
[Graphical view]
PfamPF02544. Steroid_dh. 1 hit.
[Graphical view]
PROSITEPS50244. S5A_REDUCTASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePORED_AILME
AccessionPrimary (citable) accession number: D2HBV9
Entry history
Integrated into UniProtKB/Swiss-Prot: October 5, 2010
Last sequence update: February 9, 2010
Last modified: February 19, 2014
This is version 23 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways