SubmitCancel

Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

D2HBJ8

- UBP44_AILME

UniProt

D2HBJ8 - UBP44_AILME

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein
Ubiquitin carboxyl-terminal hydrolase 44
Gene
USP44, PANDA_007904
Organism
Ailuropoda melanoleuca (Giant panda)
Status
Reviewed - Annotation score: 4 out of 5 - Protein inferred from homologyi

Functioni

Deubiquitinase that plays a key regulatory role in the spindle assembly checkpoint or mitotic checkpoint by preventing premature anaphase onset. Acts by specifically mediating deubiquitination of CDC20, a negative regulator of the anaphase promoting complex/cyclosome (APC/C). Deubiquitination of CDC20 leads to stabilize the MAD2L1-CDC20-APC/C ternary complex (also named mitotic checkpoint complex), thereby preventing premature activation of the APC/C. Promotes association of MAD2L1 with CDC20 and reinforces the spindle assembly checkpoint. Acts as a negative regulator of histone H2B (H2BK120ub1) ubiquitination By similarity.

Catalytic activityi

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei281 – 2811Nucleophile By similarity
Active sitei635 – 6351Proton acceptor By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri27 – 8862UBP-type
Add
BLAST

GO - Molecular functioni

  1. ubiquitin thiolesterase activity Source: UniProtKB
  2. ubiquitin-specific protease activity Source: UniProtKB
  3. zinc ion binding Source: InterPro
Complete GO annotation...

GO - Biological processi

  1. anaphase Source: UniProtKB
  2. mitotic nuclear division Source: UniProtKB-KW
  3. negative regulation of mitotic anaphase-promoting complex activity Source: UniProtKB
  4. proteasome-mediated ubiquitin-dependent protein catabolic process Source: UniProtKB
  5. protein deubiquitination Source: UniProtKB
  6. regulation of mitotic cell cycle spindle assembly checkpoint Source: UniProtKB
  7. regulation of spindle checkpoint Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Thiol protease

Keywords - Biological processi

Cell cycle, Cell division, Mitosis, Ubl conjugation pathway

Keywords - Ligandi

Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin carboxyl-terminal hydrolase 44 (EC:3.4.19.12)
Alternative name(s):
Deubiquitinating enzyme 44
Ubiquitin thioesterase 44
Ubiquitin-specific-processing protease 44
Gene namesi
Name:USP44
ORF Names:PANDA_007904
OrganismiAiluropoda melanoleuca (Giant panda)
Taxonomic identifieri9646 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCarnivoraCaniformiaUrsidaeAiluropoda
ProteomesiUP000008912: Unplaced

Subcellular locationi

Nucleus By similarity
Note: Peaks in interphase, with relatively low levels maintained throughout mitosis By similarity.

GO - Cellular componenti

  1. nucleus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 711711Ubiquitin carboxyl-terminal hydrolase 44
PRO_0000395810Add
BLAST

Post-translational modificationi

Dephosphorylated by CTDP1 By similarity.
Ubiquitinated; undergoes both 'Lys-48'- and 'Lys-63'-linked polyubiquitination and is degraded by the proteasome By similarity.

Keywords - PTMi

Ubl conjugation

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini272 – 677406USP
Add
BLAST

Sequence similaritiesi

Contains 1 USP domain.

Keywords - Domaini

Zinc-finger

Phylogenomic databases

GeneTreeiENSGT00750000117363.
HOGENOMiHOG000015084.
KOiK11834.
OMAiAIKSQNY.
OrthoDBiEOG7JX33S.
TreeFamiTF315281.

Family and domain databases

Gene3Di3.30.40.10. 1 hit.
InterProiIPR018200. Pept_C19ubi-hydrolase_C_CS.
IPR001394. Peptidase_C19_UCH.
IPR028889. UCH/PAN2.
IPR013083. Znf_RING/FYVE/PHD.
IPR001607. Znf_UBP.
[Graphical view]
PfamiPF00443. UCH. 1 hit.
PF02148. zf-UBP. 1 hit.
[Graphical view]
SMARTiSM00290. ZnF_UBP. 1 hit.
[Graphical view]
PROSITEiPS00972. USP_1. 1 hit.
PS00973. USP_2. 1 hit.
PS50235. USP_3. 1 hit.
PS50271. ZF_UBP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

D2HBJ8-1 [UniParc]FASTAAdd to Basket

« Hide

MLTMDKCKHI GQLRLAQDHS ILNPQKWHCV DCNTTESIWA CLSCSHVACG    50
RYIEEHALKH FQESSHPVAL EVNEMYVFCY LCDDYVLNDN ATGDLKLLRS 100
MLSAIKSQNY QCTTRSGRVL RSMGTSDDTY YLHDGTQSLL QNEDQMYTAL 150
WHRRRILMSK IFRTWFEQSP TGRKRQEEQF QEKIAKREVK KRRQELEYQV 200
KAELETIHPR KSLRLQGLAQ STTVEIVPVQ VPLQTPASPA KDKVVSTSED 250
VRLKKASDSS GKRRPIVTPG VTGLRNLGNT CYMNSVLQVL SHLLIFRQCF 300
LKLDLNQWLA VTASDKTRSP YKHPSITDTV YQMNECQETD TGSAPSRHPS 350
LSLGLSGGAS KSRKMELIQP REPSSQYISL CHELHTLFQV MWSGKWALVS 400
PFAMLHSVWR LIPAFRGYAQ QDAQEFLCEL LDKIQHELET TGTRLPALIP 450
TSQRKLIKQV LNVVNNIFHG QLLSQVTCLA CDNKSNTIEP FWDLSLEFPE 500
RYQCNGKDIA SQPCRVTEML AKFTETEALE GKIYVCDHCN SKRRRFSSKS 550
VVLTEAQKQL MICHLPQVLR LHLKRFRWSG RNNREKIGVH VGFEEILNME 600
PYCCRESLKS LRPECFIYDL SAVVMHHGKG FGSGHYTAYC YNSEGGFWVH 650
CNDSKLSMCT MDEVCKAQAY ILFYTQRVTE NGHSKLLPPE LLSGSQHPNE 700
EADTSSNEIL S 711
Length:711
Mass (Da):81,364
Last modified:February 9, 2010 - v1
Checksum:i00C0350EA2E949E1
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
GL192660 Genomic DNA. Translation: EFB15980.1.
RefSeqiXP_002919284.1. XM_002919238.1.

Genome annotation databases

EnsembliENSAMET00000002387; ENSAMEP00000002292; ENSAMEG00000002189.
GeneIDi100470180.
KEGGiaml:100470180.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
GL192660 Genomic DNA. Translation: EFB15980.1 .
RefSeqi XP_002919284.1. XM_002919238.1.

3D structure databases

ModBasei Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSAMET00000002387 ; ENSAMEP00000002292 ; ENSAMEG00000002189 .
GeneIDi 100470180.
KEGGi aml:100470180.

Organism-specific databases

CTDi 84101.

Phylogenomic databases

GeneTreei ENSGT00750000117363.
HOGENOMi HOG000015084.
KOi K11834.
OMAi AIKSQNY.
OrthoDBi EOG7JX33S.
TreeFami TF315281.

Family and domain databases

Gene3Di 3.30.40.10. 1 hit.
InterProi IPR018200. Pept_C19ubi-hydrolase_C_CS.
IPR001394. Peptidase_C19_UCH.
IPR028889. UCH/PAN2.
IPR013083. Znf_RING/FYVE/PHD.
IPR001607. Znf_UBP.
[Graphical view ]
Pfami PF00443. UCH. 1 hit.
PF02148. zf-UBP. 1 hit.
[Graphical view ]
SMARTi SM00290. ZnF_UBP. 1 hit.
[Graphical view ]
PROSITEi PS00972. USP_1. 1 hit.
PS00973. USP_2. 1 hit.
PS50235. USP_3. 1 hit.
PS50271. ZF_UBP. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "The sequence and de novo assembly of the giant panda genome."
    Li R., Fan W., Tian G., Zhu H., He L., Cai J., Huang Q., Cai Q., Li B., Bai Y., Zhang Z., Zhang Y., Wang W., Li J., Wei F., Li H., Jian M., Li J.
    , Zhang Z., Nielsen R., Li D., Gu W., Yang Z., Xuan Z., Ryder O.A., Leung F.C., Zhou Y., Cao J., Sun X., Fu Y., Fang X., Guo X., Wang B., Hou R., Shen F., Mu B., Ni P., Lin R., Qian W., Wang G., Yu C., Nie W., Wang J., Wu Z., Liang H., Min J., Wu Q., Cheng S., Ruan J., Wang M., Shi Z., Wen M., Liu B., Ren X., Zheng H., Dong D., Cook K., Shan G., Zhang H., Kosiol C., Xie X., Lu Z., Zheng H., Li Y., Steiner C.C., Lam T.T., Lin S., Zhang Q., Li G., Tian J., Gong T., Liu H., Zhang D., Fang L., Ye C., Zhang J., Hu W., Xu A., Ren Y., Zhang G., Bruford M.W., Li Q., Ma L., Guo Y., An N., Hu Y., Zheng Y., Shi Y., Li Z., Liu Q., Chen Y., Zhao J., Qu N., Zhao S., Tian F., Wang X., Wang H., Xu L., Liu X., Vinar T., Wang Y., Lam T.W., Yiu S.M., Liu S., Zhang H., Li D., Huang Y., Wang X., Yang G., Jiang Z., Wang J., Qin N., Li L., Li J., Bolund L., Kristiansen K., Wong G.K., Olson M., Zhang X., Li S., Yang H., Wang J., Wang J.
    Nature 463:311-317(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Entry informationi

Entry nameiUBP44_AILME
AccessioniPrimary (citable) accession number: D2HBJ8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 13, 2010
Last sequence update: February 9, 2010
Last modified: September 3, 2014
This is version 30 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi