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D2HBJ8 (UBP44_AILME) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 29. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Ubiquitin carboxyl-terminal hydrolase 44

EC=3.4.19.12
Alternative name(s):
Deubiquitinating enzyme 44
Ubiquitin thioesterase 44
Ubiquitin-specific-processing protease 44
Gene names
Name:USP44
ORF Names:PANDA_007904
OrganismAiluropoda melanoleuca (Giant panda) [Reference proteome]
Taxonomic identifier9646 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCarnivoraCaniformiaUrsidaeAiluropoda

Protein attributes

Sequence length711 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Deubiquitinase that plays a key regulatory role in the spindle assembly checkpoint or mitotic checkpoint by preventing premature anaphase onset. Acts by specifically mediating deubiquitination of CDC20, a negative regulator of the anaphase promoting complex/cyclosome (APC/C). Deubiquitination of CDC20 leads to stabilize the MAD2L1-CDC20-APC/C ternary complex (also named mitotic checkpoint complex), thereby preventing premature activation of the APC/C. Promotes association of MAD2L1 with CDC20 and reinforces the spindle assembly checkpoint. Acts as a negative regulator of histone H2B (H2BK120ub1) ubiquitination By similarity.

Catalytic activity

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Subcellular location

Nucleus By similarity. Note: Peaks in interphase, with relatively low levels maintained throughout mitosis By similarity.

Post-translational modification

Dephosphorylated by CTDP1 By similarity.

Ubiquitinated; undergoes both 'Lys-48'- and 'Lys-63'-linked polyubiquitination and is degraded by the proteasome By similarity.

Sequence similarities

Belongs to the peptidase C19 family. USP44 subfamily.

Contains 1 UBP-type zinc finger.

Contains 1 USP domain.

Ontologies

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 711711Ubiquitin carboxyl-terminal hydrolase 44
PRO_0000395810

Regions

Domain272 – 677406USP
Zinc finger27 – 8862UBP-type

Sites

Active site2811Nucleophile By similarity
Active site6351Proton acceptor By similarity

Sequences

Sequence LengthMass (Da)Tools
D2HBJ8 [UniParc].

Last modified February 9, 2010. Version 1.
Checksum: 00C0350EA2E949E1

FASTA71181,364
        10         20         30         40         50         60 
MLTMDKCKHI GQLRLAQDHS ILNPQKWHCV DCNTTESIWA CLSCSHVACG RYIEEHALKH 

        70         80         90        100        110        120 
FQESSHPVAL EVNEMYVFCY LCDDYVLNDN ATGDLKLLRS MLSAIKSQNY QCTTRSGRVL 

       130        140        150        160        170        180 
RSMGTSDDTY YLHDGTQSLL QNEDQMYTAL WHRRRILMSK IFRTWFEQSP TGRKRQEEQF 

       190        200        210        220        230        240 
QEKIAKREVK KRRQELEYQV KAELETIHPR KSLRLQGLAQ STTVEIVPVQ VPLQTPASPA 

       250        260        270        280        290        300 
KDKVVSTSED VRLKKASDSS GKRRPIVTPG VTGLRNLGNT CYMNSVLQVL SHLLIFRQCF 

       310        320        330        340        350        360 
LKLDLNQWLA VTASDKTRSP YKHPSITDTV YQMNECQETD TGSAPSRHPS LSLGLSGGAS 

       370        380        390        400        410        420 
KSRKMELIQP REPSSQYISL CHELHTLFQV MWSGKWALVS PFAMLHSVWR LIPAFRGYAQ 

       430        440        450        460        470        480 
QDAQEFLCEL LDKIQHELET TGTRLPALIP TSQRKLIKQV LNVVNNIFHG QLLSQVTCLA 

       490        500        510        520        530        540 
CDNKSNTIEP FWDLSLEFPE RYQCNGKDIA SQPCRVTEML AKFTETEALE GKIYVCDHCN 

       550        560        570        580        590        600 
SKRRRFSSKS VVLTEAQKQL MICHLPQVLR LHLKRFRWSG RNNREKIGVH VGFEEILNME 

       610        620        630        640        650        660 
PYCCRESLKS LRPECFIYDL SAVVMHHGKG FGSGHYTAYC YNSEGGFWVH CNDSKLSMCT 

       670        680        690        700        710 
MDEVCKAQAY ILFYTQRVTE NGHSKLLPPE LLSGSQHPNE EADTSSNEIL S 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
GL192660 Genomic DNA. Translation: EFB15980.1.
RefSeqXP_002919284.1. XM_002919238.1.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSAMET00000002387; ENSAMEP00000002292; ENSAMEG00000002189.
GeneID100470180.
KEGGaml:100470180.

Organism-specific databases

CTD84101.

Phylogenomic databases

GeneTreeENSGT00750000117363.
HOGENOMHOG000015084.
KOK11834.
OMAAIKSQNY.
OrthoDBEOG7JX33S.
TreeFamTF315281.

Family and domain databases

Gene3D3.30.40.10. 1 hit.
InterProIPR018200. Pept_C19ubi-hydrolase_C_CS.
IPR001394. Peptidase_C19_UCH.
IPR028889. UCH/PAN2.
IPR013083. Znf_RING/FYVE/PHD.
IPR001607. Znf_UBP.
[Graphical view]
PfamPF00443. UCH. 1 hit.
PF02148. zf-UBP. 1 hit.
[Graphical view]
SMARTSM00290. ZnF_UBP. 1 hit.
[Graphical view]
PROSITEPS00972. USP_1. 1 hit.
PS00973. USP_2. 1 hit.
PS50235. USP_3. 1 hit.
PS50271. ZF_UBP. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameUBP44_AILME
AccessionPrimary (citable) accession number: D2HBJ8
Entry history
Integrated into UniProtKB/Swiss-Prot: July 13, 2010
Last sequence update: February 9, 2010
Last modified: April 16, 2014
This is version 29 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Peptidase families

Classification of peptidase families and list of entries