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D2H526 (CDK12_AILME) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 35. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Cyclin-dependent kinase 12

EC=2.7.11.22
EC=2.7.11.23
Alternative name(s):
Cell division protein kinase 12
Gene names
Name:CDK12
ORF Names:PANDA_004952
OrganismAiluropoda melanoleuca (Giant panda) [Reference proteome]
Taxonomic identifier9646 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCarnivoraCaniformiaUrsidaeAiluropoda

Protein attributes

Sequence length1491 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Cyclin-dependent kinase that phosphorylates the C-terminal domain (CTD) of the large subunit of RNA polymerase II (POLR2A), thereby acting as a key regulator of transcription elongation. Regulates the expression of genes involved in DNA repair and is required for the maintenance of genomic stability. Preferentially phosphorylates 'Ser-5' in CTD repeats that are already phosphorylated at 'Ser-7', but can also phosphorylate 'Ser-2'. Required for RNA splicing, possibly by phosphorylating SRSF1/SF2. Involved in regulation of MAP kinase activity, possibly leading to affect the response to estrogen inhibitors By similarity.

Catalytic activity

ATP + [DNA-directed RNA polymerase] = ADP + [DNA-directed RNA polymerase] phosphate.

ATP + a protein = ADP + a phosphoprotein.

Subunit structure

Interacts with CCNL1 and CCNL2 By similarity.

Subcellular location

Nucleus By similarity. Nucleus speckle By similarity. Note: Colocalized with nuclear speckles throughout interphase By similarity.

Post-translational modification

Phosphorylation at Thr-894 increases kinase activity By similarity.

Sequence similarities

Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.

Contains 1 protein kinase domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 14911491Cyclin-dependent kinase 12
PRO_0000406957

Regions

Domain728 – 1021294Protein kinase
Nucleotide binding734 – 7429ATP By similarity
Nucleotide binding815 – 8206ATP By similarity
Compositional bias137 – 395259Ser-rich
Compositional bias408 – 4147Poly-Ala
Compositional bias527 – 708182Pro-rich
Compositional bias1239 – 128143Pro-rich

Sites

Active site8601Proton acceptor By similarity
Binding site7571ATP By similarity
Binding site10411ATP By similarity

Amino acid modifications

Modified residue571Phosphothreonine By similarity
Modified residue731Phosphotyrosine By similarity
Modified residue2371Phosphoserine By similarity
Modified residue2501Phosphoserine By similarity
Modified residue2751Phosphoserine By similarity
Modified residue2771Phosphoserine By similarity
Modified residue3021Phosphoserine By similarity
Modified residue3041Phosphoserine By similarity
Modified residue3111Phosphoserine By similarity
Modified residue3131Phosphoserine By similarity
Modified residue3191Phosphoserine By similarity
Modified residue3241Phosphoserine By similarity
Modified residue3261Phosphoserine By similarity
Modified residue3331Phosphoserine By similarity
Modified residue3341Phosphoserine By similarity
Modified residue3351Phosphoserine By similarity
Modified residue3391Phosphoserine By similarity
Modified residue3461Phosphoserine By similarity
Modified residue3841Phosphoserine By similarity
Modified residue3861Phosphoserine By similarity
Modified residue4011Phosphoserine By similarity
Modified residue4211Phosphoserine By similarity
Modified residue4241Phosphoserine By similarity
Modified residue5151Phosphothreonine By similarity
Modified residue6821Phosphoserine By similarity
Modified residue6861Phosphoserine By similarity
Modified residue6931Phosphothreonine By similarity
Modified residue8941Phosphothreonine By similarity
Modified residue10541Phosphoserine By similarity
Modified residue10841Phosphoserine By similarity
Modified residue12451Phosphothreonine By similarity

Sequences

Sequence LengthMass (Da)Tools
D2H526 [UniParc].

Last modified February 9, 2010. Version 1.
Checksum: BA53304B554D1195

FASTA1,491164,479
        10         20         30         40         50         60 
MPNPERHGGK KDGSGGASGT LQPSSGGGSS NSRERHRLVS KHKRHKSKHS KDMGLVTPEA 

        70         80         90        100        110        120 
APLGTIIKPL VEYDDISSDS DTFSDDMAFK LDRRENDERR GTDRSDRLHK HRHHQHRRSR 

       130        140        150        160        170        180 
DLLKTKQTEK EKNQEVSSKS GSMKDRISGS SKRSNEENED YGKAQISKSS SNKESRSSKL 

       190        200        210        220        230        240 
HKEKTRKERE LKSGHKDRSK SHRKRETPKS YKTVDSPKRR SRSPHRKWSD SPKQDDSPSG 

       250        260        270        280        290        300 
ASYGQDYDLS PPRSHTSSNY DSYKKSPGST SRRQSISPPY KEPSAYQSST RSPSPYSRRQ 

       310        320        330        340        350        360 
RSVSPYSRRR SSSYERSGSY SGRSPSPYGR RRSSSPFMSK RSLSRSPLPS RKSMKSRSRS 

       370        380        390        400        410        420 
PAYSRHSSSH SKKKRSGSRS RHSSISPVRL PLNSSLGAEL SRKKKERAAA AAAAKMDGKE 

       430        440        450        460        470        480 
SKGSPIFLPR KENSLVEAKD SGLESKKLTR GVKLEKSAPD TELVNIPHLN TEVKNSLDTG 

       490        500        510        520        530        540 
KVKLDENSEK HPIKDLKAQG SRDSKPIALK EEIVTPKETE TSEKETPPPV PAVTSPPPPL 

       550        560        570        580        590        600 
PTTSPPPQTP PLPPLPPLPA IPQQPPLPPP QPAFSHVLAS STSTLPPSTH PRTSTLSSQA 

       610        620        630        640        650        660 
NSQPLAQVSV KTQVSVTAAI PHLKTSTLPP LPLPPLLPGD DDMDSPKETP PSKPVKKEKE 

       670        680        690        700        710        720 
QRPRHLLTDL PLPPELPGGD PSPPDSPEPK AVTPPQQPYK KRPKICCPRY GERRQTESDW 

       730        740        750        760        770        780 
GKRCVDKFDI IGIIGEGTYG QVYKAKDKDT GELVALKKVR LDNEKEGFPI TAIREIKILR 

       790        800        810        820        830        840 
QLIHRSVVNM KEIVTDKQDA LDFKKDKGAF YLVFEYMDHD LMGLLESGLV HFSEDHIKSF 

       850        860        870        880        890        900 
MKQLMEGLDY CHKKNFLHRD IKCSNILLNN SGQIKLADFG LARLYNSEES RPYTNKVITL 

       910        920        930        940        950        960 
WYRPPELLLG EERYTPAIDV WSCGCILGEL FTKKPIFQAN LELAQLELIS RLCGSPCPAV 

       970        980        990       1000       1010       1020 
WPDVIKLPYF NTMKPKKQYR RRLREEFSFI PSAALDLLDH MLTLDPSKRC TAEQTLQSDF 

      1030       1040       1050       1060       1070       1080 
LKDVELSKMD PPDLPHWQDC HELWSKKRRR QRQSGVVIEE PPPSKASRKE TTSGTSAEPV 

      1090       1100       1110       1120       1130       1140 
KNSSPAPPQP ASGKVEPGTG DAIGLGDITQ QLNQSELAVL LNLLQSQTDL SIPQMAQLLN 

      1150       1160       1170       1180       1190       1200 
IHSNPEMQQQ LEALNQSISA LTEATSQQQD SEHMAPEESL KEAPPALVVQ PSAEQTTSEA 

      1210       1220       1230       1240       1250       1260 
SSTPADMQNM LAVLLSQLMK TQEPAGSLEE NNSDKNSGPQ GPRRTPTMPQ EEAAACPPHI 

      1270       1280       1290       1300       1310       1320 
LPPEKRPPEP PGPPPPPPPP PLIEGDLSSA PQELNPAVTA ALLQLLSQPE AEPPGHLPHE 

      1330       1340       1350       1360       1370       1380 
HQALRPMEYS TRPHPNRTYG NTDGPETGFS ATDTDERNSG PALTESLTQT LVKNRTFSGS 

      1390       1400       1410       1420       1430       1440 
VSHLGESSSY QGTGSVQFPG DQDLRFARVP LPLHSVVGQP FLKAEGSSNS VVHAETKLQN 

      1450       1460       1470       1480       1490 
YGELGPGTTG ASSSGAGLNW GGSAQSSAYG KLYRGPTRVP PRGGRGRGVP Y 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
GL192497 Genomic DNA. Translation: EFB19924.1.
RefSeqXP_002916839.1. XM_002916793.1.

3D structure databases

ProteinModelPortalD2H526.
ModBaseSearch...
MobiDBSearch...

Proteomic databases

PRIDED2H526.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSAMET00000004348; ENSAMEP00000004181; ENSAMEG00000003944.
GeneID100467815.
KEGGaml:100467815.

Organism-specific databases

CTD51755.

Phylogenomic databases

GeneTreeENSGT00740000114964.
HOGENOMHOG000049118.
KOK08819.
OMAEEIATPK.
OrthoDBEOG76DTSM.
TreeFamTF101060.

Family and domain databases

InterProIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMSSF56112. SSF56112. 1 hit.
PROSITEPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameCDK12_AILME
AccessionPrimary (citable) accession number: D2H526
Entry history
Integrated into UniProtKB/Swiss-Prot: April 5, 2011
Last sequence update: February 9, 2010
Last modified: June 11, 2014
This is version 35 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families