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Protein
Submitted name:

Alpha-amylase

Gene

amyL

Organism
Lactococcus lactis subsp. lactis (strain KF147)
Status
Unreviewed-Annotation score: Annotation score: 1 out of 5-Protein predictedi

Functioni

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi102Calcium 1UniRule annotation1
Metal bindingi181Calcium 2UniRule annotation1
Metal bindingi192Calcium 1UniRule annotation1
Metal bindingi198Calcium 1UniRule annotation1
Metal bindingi200Calcium 2UniRule annotation1
Active sitei229NucleophileUniRule annotation1
Metal bindingi233Calcium 1; via carbonyl oxygenUniRule annotation1
Active sitei259Proton donorUniRule annotation1
Metal bindingi298Calcium 3; via carbonyl oxygenUniRule annotation1
Metal bindingi402Calcium 3UniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionGlycosidaseImported, Hydrolase
LigandCalciumUniRule annotation, Metal-bindingUniRule annotation

Protein family/group databases

CAZyiGH13. Glycoside Hydrolase Family 13.

Names & Taxonomyi

Protein namesi
Submitted name:
Alpha-amylaseImported (EC:3.2.1.1Imported)
Gene namesi
Name:amyLImported
Ordered Locus Names:LLKF_1316Imported
OrganismiLactococcus lactis subsp. lactis (strain KF147)Imported
Taxonomic identifieri684738 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeLactococcus
Proteomesi
  • UP000001886 Componenti: Chromosome

Structurei

3D structure databases

ProteinModelPortaliD2BR19.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini2 – 386AamyInterPro annotationAdd BLAST385

Phylogenomic databases

HOGENOMiHOG000094847.
KOiK01176.
OMAiFFHWYYP.

Family and domain databases

Gene3Di2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
InterProiView protein in InterPro
IPR013776. A-amylase_thermo.
IPR006047. Glyco_hydro_13_cat_dom.
IPR013780. Glyco_hydro_b.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
PfamiView protein in Pfam
PF00128. Alpha-amylase. 1 hit.
PIRSFiPIRSF001021. Alph-amls_thrmst. 1 hit.
SMARTiView protein in SMART
SM00642. Aamy. 1 hit.
SUPFAMiSSF51445. SSF51445. 1 hit.

Sequencei

Sequence statusi: Complete.

D2BR19-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKTILQAFEW YLPSDSQHWN NIKENIPDLG KLGFSGLWLP PASKAASGVE
60 70 80 90 100
DVGYGTYDLF DLGEFDQKGT IPTKYGTKDE YLDLINTLHH NNIEVYADIV
110 120 130 140 150
FNHMMGADET ETIEADIKAE DNHLHNIENN KTVEVWTKFT FPGRQGKYDN
160 170 180 190 200
YIWTWHNFTG IDYDERKNQE EILEFEGHEW DENVDSENNN FDYLMGADLD
210 220 230 240 250
FSVSETVEQL EKWGHWFSEM TKIDGFRLDA IKHIDFKYFD KWLEQRAKQL
260 270 280 290 300
DKKLFIVGEY WSDDLGKLEY YLEQSGDKIQ LFDVPLHFNM KEASSSNGEF
310 320 330 340 350
DMRTLFDHTL TASQPELSVT FVDNHDTQEG QALESWIPAW FKEHAYSLIL
360 370 380 390 400
LRKKGTPTVF WGDLYGIHSR NVNPVGDNLR TMIALRKDSE FLRENDYFDH
410 420 430 440 450
PDIIGWTNIL KIDNKEYGLS CILTNKNGGS KYMIIDKAYA GKVYIDLFGR
460 470 480 490
HEIPITLDQN GGAEFYVNDG SVSVWVDKEI VSKIDQMNFQ N
Length:491
Mass (Da):57,088
Last modified:February 9, 2010 - v1
Checksum:iBFAF30BEFF2DF7D5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001834 Genomic DNA. Translation: ADA64994.1.
RefSeqiWP_012897858.1. NC_013656.1.

Genome annotation databases

EnsemblBacteriaiADA64994; ADA64994; LLKF_1316.
KEGGillk:LLKF_1316.
PATRICi32251126. VBILacLac141273_1335.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001834 Genomic DNA. Translation: ADA64994.1.
RefSeqiWP_012897858.1. NC_013656.1.

3D structure databases

ProteinModelPortaliD2BR19.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

CAZyiGH13. Glycoside Hydrolase Family 13.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiADA64994; ADA64994; LLKF_1316.
KEGGillk:LLKF_1316.
PATRICi32251126. VBILacLac141273_1335.

Phylogenomic databases

HOGENOMiHOG000094847.
KOiK01176.
OMAiFFHWYYP.

Family and domain databases

Gene3Di2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
InterProiView protein in InterPro
IPR013776. A-amylase_thermo.
IPR006047. Glyco_hydro_13_cat_dom.
IPR013780. Glyco_hydro_b.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
PfamiView protein in Pfam
PF00128. Alpha-amylase. 1 hit.
PIRSFiPIRSF001021. Alph-amls_thrmst. 1 hit.
SMARTiView protein in SMART
SM00642. Aamy. 1 hit.
SUPFAMiSSF51445. SSF51445. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiD2BR19_LACLK
AccessioniPrimary (citable) accession number: D2BR19
Entry historyiIntegrated into UniProtKB/TrEMBL: February 9, 2010
Last sequence update: February 9, 2010
Last modified: February 15, 2017
This is version 43 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteomeImported

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.