Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Arginine biosynthesis bifunctional protein ArgJ

Gene

argJ

Organism
Lactococcus lactis subsp. lactis (strain KF147)
Status
Unreviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes two activities which are involved in the cyclic version of arginine biosynthesis: the synthesis of N-acetylglutamate from glutamate and acetyl-CoA as the acetyl donor, and of ornithine by transacetylation between N2-acetylornithine and glutamate.UniRule annotation

Miscellaneous

Some bacteria possess a monofunctional ArgJ, i.e., capable of catalyzing only the fifth step of the arginine biosynthetic pathway.UniRule annotation

Catalytic activityi

Acetyl-CoA + L-glutamate = CoA + N-acetyl-L-glutamate.UniRule annotation
N2-acetyl-L-ornithine + L-glutamate = L-ornithine + N-acetyl-L-glutamate.UniRule annotation

Pathwayi: L-arginine biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes L-ornithine and N-acetyl-L-glutamate from L-glutamate and N(2)-acetyl-L-ornithine (cyclic).UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Arginine biosynthesis bifunctional protein ArgJ (argJ)
This subpathway is part of the pathway L-arginine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-ornithine and N-acetyl-L-glutamate from L-glutamate and N(2)-acetyl-L-ornithine (cyclic), the pathway L-arginine biosynthesis and in Amino-acid biosynthesis.

Pathwayi: L-arginine biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes N(2)-acetyl-L-ornithine from L-glutamate.UniRule annotation
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. Arginine biosynthesis bifunctional protein ArgJ (argJ)
  2. Acetylglutamate kinase (argB)
  3. N-acetyl-gamma-glutamyl-phosphate reductase (argC)
  4. Acetylornithine aminotransferase (argD)
This subpathway is part of the pathway L-arginine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes N(2)-acetyl-L-ornithine from L-glutamate, the pathway L-arginine biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei112Involved in the stabilization of negative charge on the oxyanion by the formation of the oxyanion holeUniRule annotation1
Sitei113Involved in the stabilization of negative charge on the oxyanion by the formation of the oxyanion holeUniRule annotation1
Binding sitei147SubstrateUniRule annotation1
Binding sitei173SubstrateUniRule annotation1
Active sitei184NucleophileUniRule annotation1
Binding sitei184SubstrateUniRule annotation1
Binding sitei270SubstrateUniRule annotation1
Binding sitei391SubstrateUniRule annotation1
Binding sitei396SubstrateUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAcyltransferaseUniRule annotationImported, Transferase
Biological processAmino-acid biosynthesis, Arginine biosynthesisUniRule annotation

Enzyme and pathway databases

UniPathwayiUPA00068; UER00106.
UPA00068; UER00111.

Names & Taxonomyi

Protein namesi
Recommended name:
Arginine biosynthesis bifunctional protein ArgJUniRule annotation
Cleaved into the following 2 chains:
Arginine biosynthesis bifunctional protein ArgJ alpha chainUniRule annotation
Arginine biosynthesis bifunctional protein ArgJ beta chainUniRule annotation
Including the following 2 domains:
Glutamate N-acetyltransferaseUniRule annotation (EC:2.3.1.35UniRule annotation)
Alternative name(s):
Ornithine acetyltransferaseUniRule annotation
Short name:
OATaseUniRule annotation
Ornithine transacetylaseUniRule annotation
Amino-acid acetyltransferaseUniRule annotation (EC:2.3.1.1UniRule annotation)
Alternative name(s):
N-acetylglutamate synthaseUniRule annotation
Short name:
AGSaseUniRule annotation
Gene namesi
Name:argJUniRule annotationImported
Ordered Locus Names:LLKF_0818Imported
OrganismiLactococcus lactis subsp. lactis (strain KF147)Imported
Taxonomic identifieri684738 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeLactococcus
Proteomesi
  • UP000001886 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

CytoplasmUniRule annotation

PTM / Processingi

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei183 – 184Cleavage; by autolysisUniRule annotation2

Keywords - PTMi

Autocatalytic cleavageUniRule annotation

Interactioni

Subunit structurei

Heterotetramer of two alpha and two beta chains.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliD2BPS8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the ArgJ family.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000022797.
KOiK00620.
OMAiVIGRPYP.

Family and domain databases

CDDicd02152. OAT. 1 hit.
Gene3Di3.60.70.12. 1 hit.
HAMAPiMF_01106. ArgJ. 1 hit.
InterProiView protein in InterPro
IPR002813. Arg_biosynth_ArgJ.
IPR016117. ArgJ-like_dom.
PANTHERiPTHR23100. PTHR23100. 1 hit.
PfamiView protein in Pfam
PF01960. ArgJ. 1 hit.
SUPFAMiSSF56266. SSF56266. 1 hit.
TIGRFAMsiTIGR00120. ArgJ. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

D2BPS8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKEIKGTIAS PKGFLADAVH AQLKYKNLDL GLILSQVPAA IAGVFTTNKV
60 70 80 90 100
CAAPVLIDRQ IVKNGQARAI ICNSAVANAV TGEQGYANAL KTQKLLAEKF
110 120 130 140 150
ELKAEEVAVC STGVIGVQLP MEKIATGISK LSQNEGTAAY FAKAILTTDT
160 170 180 190 200
QTKTINFEAE IGGQIVNMAG VCKGSGMIHP NMATMLAFIT TDAKIAQALL
210 220 230 240 250
QKTLSEIIET TFNQITVDGD TSTNDTVLLM ANGQAKNNEI LEGSSDYLLF
260 270 280 290 300
KEMLAKVCQS LAKQIAADGE GATKLIEVTV KGAPNDLAAR FIAKKIVGSS
310 320 330 340 350
LVKTAIFGAD PNWGRIISSI GQVANFEVSD IELKLQDELV LYHSTPVDFD
360 370 380 390
AAFLSEKLKE DKIEIIADLN AGSGLGQAWG CDLTYKYVEI NALYTS
Length:396
Mass (Da):42,130
Last modified:February 9, 2010 - v1
Checksum:i51534E125DABCBF7
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001834 Genomic DNA. Translation: ADA64553.1.
RefSeqiWP_011835544.1. NC_013656.1.

Genome annotation databases

EnsemblBacteriaiADA64553; ADA64553; LLKF_0818.
KEGGillk:LLKF_0818.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiD2BPS8_LACLK
AccessioniPrimary (citable) accession number: D2BPS8
Entry historyiIntegrated into UniProtKB/TrEMBL: February 9, 2010
Last sequence update: February 9, 2010
Last modified: June 7, 2017
This is version 61 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteomeImported, Multifunctional enzymeUniRule annotation