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D2BPS8

- D2BPS8_LACLK

UniProt

D2BPS8 - D2BPS8_LACLK

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Protein

Arginine biosynthesis bifunctional protein ArgJ

Gene
argJ, LLKF_0818
Organism
Lactococcus lactis subsp. lactis (strain KF147)
Status
Unreviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes two activities which are involved in the cyclic version of arginine biosynthesis: the synthesis of N-acetylglutamate from glutamate and acetyl-CoA as the acetyl donor, and of ornithine by transacetylation between N(2)-acetylornithine and glutamate By similarity.UniRule annotation

Catalytic activityi

Acetyl-CoA + L-glutamate = CoA + N-acetyl-L-glutamate.UniRule annotation
N(2)-acetyl-L-ornithine + L-glutamate = L-ornithine + N-acetyl-L-glutamate.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei112 – 1121Involved in the stabilization of negative charge on the oxyanion by the formation of the oxyanion hole By similarityUniRule annotation
Sitei113 – 1131Involved in the stabilization of negative charge on the oxyanion by the formation of the oxyanion hole By similarityUniRule annotation
Binding sitei147 – 1471Substrate By similarityUniRule annotation
Binding sitei173 – 1731Substrate By similarityUniRule annotation
Sitei183 – 1842Cleavage; by autolysis By similarityUniRule annotation
Active sitei184 – 1841Nucleophile By similarityUniRule annotation
Binding sitei184 – 1841Substrate By similarityUniRule annotation
Binding sitei270 – 2701Substrate By similarityUniRule annotation
Binding sitei391 – 3911Substrate By similarityUniRule annotation
Binding sitei396 – 3961Substrate By similarityUniRule annotation

GO - Molecular functioni

  1. acetyl-CoA:L-glutamate N-acetyltransferase activity Source: UniProtKB-HAMAP
  2. glutamate N-acetyltransferase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. arginine biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

AcyltransferaseUniRule annotationImported, Transferase

Keywords - Biological processi

Amino-acid biosynthesis, Arginine biosynthesisUniRule annotation

Enzyme and pathway databases

BioCyciLLAC684738:GI3F-858-MONOMER.
UniPathwayiUPA00068; UER00106.
UPA00068; UER00111.

Protein family/group databases

MEROPSiT05.001.

Names & Taxonomyi

Protein namesi
Recommended name:
Arginine biosynthesis bifunctional protein ArgJUniRule annotation
Gene namesi
Name:argJUniRule annotationImported
Ordered Locus Names:LLKF_0818Imported
OrganismiLactococcus lactis subsp. lactis (strain KF147)Imported
Taxonomic identifieri684738 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeLactococcus
ProteomesiUP000001886: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

CytoplasmUniRule annotation

PTM / Processingi

Keywords - PTMi

Autocatalytic cleavageUniRule annotation

Interactioni

Subunit structurei

Heterotetramer of two alpha and two beta chains By similarity.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliD2BPS8.
SMRiD2BPS8. Positions 184-394.

Family & Domainsi

Sequence similaritiesi

Belongs to the ArgJ family.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000022797.
KOiK00620.
OMAiVTVHIAG.
OrthoDBiEOG6P8TQQ.

Family and domain databases

Gene3Di3.60.70.12. 1 hit.
HAMAPiMF_01106. ArgJ.
InterProiIPR002813. Arg_biosynth_ArgJ.
IPR016117. ArgJ-like_dom.
[Graphical view]
PANTHERiPTHR23100. PTHR23100. 1 hit.
PfamiPF01960. ArgJ. 1 hit.
[Graphical view]
SUPFAMiSSF56266. SSF56266. 1 hit.
TIGRFAMsiTIGR00120. ArgJ. 1 hit.

Sequencei

Sequence statusi: Complete.

D2BPS8-1 [UniParc]FASTAAdd to Basket

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MKEIKGTIAS PKGFLADAVH AQLKYKNLDL GLILSQVPAA IAGVFTTNKV    50
CAAPVLIDRQ IVKNGQARAI ICNSAVANAV TGEQGYANAL KTQKLLAEKF 100
ELKAEEVAVC STGVIGVQLP MEKIATGISK LSQNEGTAAY FAKAILTTDT 150
QTKTINFEAE IGGQIVNMAG VCKGSGMIHP NMATMLAFIT TDAKIAQALL 200
QKTLSEIIET TFNQITVDGD TSTNDTVLLM ANGQAKNNEI LEGSSDYLLF 250
KEMLAKVCQS LAKQIAADGE GATKLIEVTV KGAPNDLAAR FIAKKIVGSS 300
LVKTAIFGAD PNWGRIISSI GQVANFEVSD IELKLQDELV LYHSTPVDFD 350
AAFLSEKLKE DKIEIIADLN AGSGLGQAWG CDLTYKYVEI NALYTS 396
Length:396
Mass (Da):42,130
Last modified:February 9, 2010 - v1
Checksum:i51534E125DABCBF7
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP001834 Genomic DNA. Translation: ADA64553.1.
RefSeqiYP_003353274.1. NC_013656.1.

Genome annotation databases

EnsemblBacteriaiADA64553; ADA64553; LLKF_0818.
GeneIDi8678239.
KEGGillk:LLKF_0818.
PATRICi32250113. VBILacLac141273_0830.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP001834 Genomic DNA. Translation: ADA64553.1 .
RefSeqi YP_003353274.1. NC_013656.1.

3D structure databases

ProteinModelPortali D2BPS8.
SMRi D2BPS8. Positions 184-394.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

MEROPSi T05.001.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ADA64553 ; ADA64553 ; LLKF_0818 .
GeneIDi 8678239.
KEGGi llk:LLKF_0818.
PATRICi 32250113. VBILacLac141273_0830.

Phylogenomic databases

HOGENOMi HOG000022797.
KOi K00620.
OMAi VTVHIAG.
OrthoDBi EOG6P8TQQ.

Enzyme and pathway databases

UniPathwayi UPA00068 ; UER00106 .
UPA00068 ; UER00111 .
BioCyci LLAC684738:GI3F-858-MONOMER.

Family and domain databases

Gene3Di 3.60.70.12. 1 hit.
HAMAPi MF_01106. ArgJ.
InterProi IPR002813. Arg_biosynth_ArgJ.
IPR016117. ArgJ-like_dom.
[Graphical view ]
PANTHERi PTHR23100. PTHR23100. 1 hit.
Pfami PF01960. ArgJ. 1 hit.
[Graphical view ]
SUPFAMi SSF56266. SSF56266. 1 hit.
TIGRFAMsi TIGR00120. ArgJ. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Genome-scale genotype-phenotype matching of two Lactococcus lactis isolates from plants identifies mechanisms of adaptation to the plant niche."
    Siezen R.J., Starrenburg M.J., Boekhorst J., Renckens B., Molenaar D., van Hylckama Vlieg J.E.
    Appl. Environ. Microbiol. 74:424-436(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: KF147Imported.

Entry informationi

Entry nameiD2BPS8_LACLK
AccessioniPrimary (citable) accession number: D2BPS8
Entry historyi
Integrated into UniProtKB/TrEMBL: February 9, 2010
Last sequence update: February 9, 2010
Last modified: September 3, 2014
This is version 36 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Miscellaneous

Some bacteria possess a monofunctional ArgJ, i.e., capable of catalyzing only the fifth step of the arginine biosynthetic pathway By similarity.UniRule annotation

Keywords - Technical termi

Complete proteome, Multifunctional enzymeUniRule annotation

External Data

Dasty 3

Similar proteinsi