thyA

Functionⁱ

Catalyzes the reductive methylation of 2'-deoxyuridine-5'-monophosphate (dUMP) to 2'-deoxythymidine-5'-monophosphate (dTMP) while utilizing 5,10-methylenetetrahydrofolate (mTHF) as the methyl donor and reductant in the reaction, yielding dihydrofolate (DHF) as a by-product. This enzymatic reaction provides an intracellular de novo source of dTMP, an essential precursor for DNA biosynthesis.UniRule annotation

Catalytic activityⁱ

5,10-methylenetetrahydrofolate + dUMP = dihydrofolate + dTMP.UniRule annotationSAAS annotation

Pathwayⁱ: dTTP biosynthesis

This protein is involved in the pathway dTTP biosynthesis, which is part of Pyrimidine metabolism.UniRule annotationSAAS annotation
View all proteins of this organism that are known to be involved in the pathway dTTP biosynthesis and in Pyrimidine metabolism.

Sites

Feature key	Position(s)	DescriptionActions	Length
Active siteⁱ	153	PROSITE-ProRule annotation	1
Active siteⁱ	153	NucleophileUniRule annotation	1
Binding siteⁱ	181	5,10-methylenetetrahydrofolateUniRule annotation	1
Binding siteⁱ	189	dUMPUniRule annotation	1
Binding siteⁱ	278	5,10-methylenetetrahydrofolate; via carbonyl oxygenUniRule annotation	1

Regions

Feature key	Position(s)	DescriptionActions	Length
Nucleotide bindingⁱ	132 – 133	dUMP; shared with dimeric partnerUniRule annotation	2
Nucleotide bindingⁱ	178 – 181	dUMPUniRule annotation	4
Nucleotide bindingⁱ	219 – 221	dUMPUniRule annotation	3

GO - Molecular functionⁱ

thymidylate synthase activity Source: UniProtKB-UniRule

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

dTMP biosynthetic process Source: UniProtKB-UniRule
dTTP biosynthetic process Source: UniProtKB-UniPathway

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	MethyltransferaseUniRule annotationSAAS annotationImported, Transferase
Biological process	Nucleotide biosynthesisUniRule annotationSAAS annotation

Enzyme and pathway databases

UniPathwayⁱ	UPA00575.

UniPathwayⁱ

UPA00575.

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Thymidylate synthaseUniRule annotationSAAS annotation (EC:2.1.1.45UniRule annotation) Short name: TSUniRule annotation Short name: TSaseUniRule annotation
Gene namesⁱ	Name:thyAUniRule annotationImported Ordered Locus Names:LLKF_1662Imported
Organismⁱ	Lactococcus lactis subsp. lactis (strain KF147)Imported
Taxonomic identifierⁱ	684738 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Bacteria › Terrabacteria group › Firmicutes › Bacilli › Lactobacillales › Streptococcaceae › Lactococcus › Lactococcus lactis › Lactococcus lactis subsp. lactis
Proteomesⁱ	UP000001886 Componentⁱ: Chromosome

Subcellular locationⁱ

Cytoplasm
UniRule annotation
Automatic assertion according to rulesⁱ
- HAMAP-Rule:MF_00008

GO - Cellular componentⁱ

cytoplasm Source: UniProtKB-SubCell

View the complete GO annotation on QuickGO ...

Keywords - Cellular componentⁱ

CytoplasmUniRule annotation

Interactionⁱ

Subunit structureⁱ

Homodimer.UniRule annotationSAAS annotation

Structureⁱ

3D structure databases

ProteinModelPortalⁱ	D2BKH9.
SMRⁱ	D2BKH9.
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Domains and Repeats

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Domainⁱ	8 – 279	Thymidylat_syntInterPro annotationAdd BLAST		272

Sequence similaritiesⁱ

Belongs to the thymidylate synthase family. Bacterial-type ThyA subfamily.UniRule annotation

Phylogenomic databases

HOGENOMⁱ	HOG000257898.
KEGG Orthology (KO) More...KOⁱ	K00560.
OMAⁱ	WNEWEVG.

Family and domain databases

Conserved Domains Database More...CDDⁱ	cd00351. TS_Pyrimidine_HMase. 1 hit.
Gene3Dⁱ	3.30.572.10. 1 hit.
HAMAPⁱ	MF_00008. Thymidy_synth_bact. 1 hit.
InterProⁱ	View protein in InterPro IPR023451. Thymidate_synth/dCMP_Mease. IPR036926. Thymidate_synth/dCMP_Mease_sf. IPR000398. Thymidylate_synthase. IPR020940. Thymidylate_synthase_AS.
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF00303. Thymidylat_synt. 1 hit.
PRINTSⁱ	PR00108. THYMDSNTHASE.
SUPFAMⁱ	SSF55831. SSF55831. 1 hit.
PROSITEⁱ	View protein in PROSITE PS00091. THYMIDYLATE_SYNTHASE. 1 hit.

Sequenceⁱ

Sequence statusⁱ: Complete.

D2BKH9-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MTYADKIFKQ NIQNILDNGV FSENARPKYK DGQTANSKYV TGSFVTYDLQ 
        60         70         80         90        100
KGEFPITTLR PIPIKSAIKE LMWIYQDQTS ELAILEEKYG VKYWGEWGIG 
       110        120        130        140        150
DGTIGQRYGA TVKKYNIIGK LLDGLAKNPW NRRNIINLWQ YEDFEETEGL 
       160        170        180        190        200
LPCAFQTMFD VRREQDGQIY LDATLIQRSN DMLVAHHINA MQYVALQMMI 
       210        220        230        240        250
AKHFSWKVGK FFYFVNNLHI YDNQFEQANE LVKRTASDKE PRLVLNVPDG 
       260        270 
TNFFDIKPED FELVDYEPVK PQLKFDLAI

Length:279

Mass (Da):32,499

Last modified:February 9, 2010 - v1

Checksum:ⁱCE0B97935D8DA1F8

GO

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	CP001834 Genomic DNA. Translation: ADA65285.1.
NCBI Reference Sequences More...RefSeqⁱ	WP_003130015.1. NC_013656.1.

Genome annotation databases

EnsemblBacteriaⁱ	ADA65285; ADA65285; LLKF_1662.
KEGGⁱ	llk:LLKF_1662.

Protein	Similar proteins	Organisms	Length	Cluster ID	Cluster name	Size
D2BKH9	P19368 A0A2A8DDY7 Q548I9 A0A199YPI2 G6FAQ2 T0V9V4 U5PPN2	Lactococcus lactis subsp. lactis (strain IL1403) (Streptococcus lactis) Lactococcus lactis Lactococcus lactis subsp. lactis (Streptococcus lactis) Lactococcus lactis RTB018 Lactococcus lactis subsp. lactis CNCM I-1631 Lactococcus lactis subsp. lactis bv. diacetylactis str. TIFN2 Lactococcus lactis subsp. lactis KLDS 4.0325	279	UniRef100_P19368	Cluster: Thymidylate synthase	8

Protein	Similar proteins	Organisms	Length	Cluster ID	Cluster name	Size
D2BKH9	P19368 A0A2A8DDY7 Q548I9 A0A199YPI2 G6FAQ2 T0V9V4 U5PPN2 A0A0B8QQD7 U6EMU3 A0A0B8QV10 +42	Lactococcus lactis subsp. lactis (strain IL1403) (Streptococcus lactis) Lactococcus lactis Lactococcus lactis subsp. lactis (Streptococcus lactis) Lactococcus lactis RTB018 Lactococcus lactis subsp. lactis CNCM I-1631 Lactococcus lactis subsp. lactis bv. diacetylactis str. TIFN2 Lactococcus lactis subsp. lactis KLDS 4.0325 Lactococcus lactis subsp. lactis Dephy 1 Lactococcus lactis subsp. cremoris KW2 Lactococcus lactis subsp. cremoris (Streptococcus cremoris) And more	279	UniRef90_P19368	Cluster: Thymidylate synthase	53

Protein	Similar proteins	Organisms	Length	Cluster ID	Cluster name	Size
D2BKH9	Q5XCM3 Q8P1D1 Q1JHC5 Q1J744 A2RF20 Q48U25 A0A2J9PH55 A0A0H3BZN7 UPI00092E41B2 UPI0007C3CF7B +769	Streptococcus pyogenes serotype M6 (strain ATCC BAA-946 / MGAS10394) Streptococcus pyogenes serotype M18 (strain MGAS8232) Streptococcus pyogenes serotype M2 (strain MGAS10270) Streptococcus pyogenes serotype M4 (strain MGAS10750) Streptococcus pyogenes serotype M5 (strain Manfredo) Streptococcus pyogenes serotype M28 (strain MGAS6180) Streptococcus pyogenes Streptococcus pyogenes serotype M49 (strain NZ131) Streptococcus pyogenes serotype M12 (strain MGAS2096) Streptococcus pyogenes serotype M12 (strain MGAS9429) And more	279	UniRef50_Q5XCM3	Cluster: Thymidylate synthase	780

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	CP001834 Genomic DNA. Translation: ADA65285.1.
NCBI Reference Sequences More...RefSeqⁱ	WP_003130015.1. NC_013656.1.

3D structure databases

ProteinModelPortalⁱ	D2BKH9.
SMRⁱ	D2BKH9.
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

EnsemblBacteriaⁱ	ADA65285; ADA65285; LLKF_1662.
KEGGⁱ	llk:LLKF_1662.

Phylogenomic databases

HOGENOMⁱ	HOG000257898.
KEGG Orthology (KO) More...KOⁱ	K00560.
OMAⁱ	WNEWEVG.

Enzyme and pathway databases

UniPathwayⁱ	UPA00575.

UniPathwayⁱ

UPA00575.

Family and domain databases

Conserved Domains Database More...CDDⁱ	cd00351. TS_Pyrimidine_HMase. 1 hit.
Gene3Dⁱ	3.30.572.10. 1 hit.
HAMAPⁱ	MF_00008. Thymidy_synth_bact. 1 hit.
InterProⁱ	View protein in InterPro IPR023451. Thymidate_synth/dCMP_Mease. IPR036926. Thymidate_synth/dCMP_Mease_sf. IPR000398. Thymidylate_synthase. IPR020940. Thymidylate_synthase_AS.
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF00303. Thymidylat_synt. 1 hit.
PRINTSⁱ	PR00108. THYMDSNTHASE.
SUPFAMⁱ	SSF55831. SSF55831. 1 hit.
PROSITEⁱ	View protein in PROSITE PS00091. THYMIDYLATE_SYNTHASE. 1 hit.
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	D2BKH9_LACLK
Accessionⁱ	D2BKH9Primary (citable) accession number: D2BKH9
Entry historyⁱ	Integrated into UniProtKB/TrEMBL:	February 9, 2010
	Last sequence update:	February 9, 2010
	Last modified:	March 28, 2018
	This is version 64 of the entry and version 1 of the sequence. See complete history.
Entry statusⁱ	Unreviewed (UniProtKB/TrEMBL)

Miscellaneousⁱ

Caution

Lacks conserved residue(s) required for the propagation of feature annotation.UniRule annotation

Keywords - Technical termⁱ

Complete proteomeImported

UniProtKB

UniParc

Help

UniRef

Proteomes

Annotation systems

Supporting data

UniProtKB - D2BKH9 (D2BKH9_LACLK)

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Thymidylate synthase

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">‘Function’</a> section describes the metabolic pathway(s) associated with a protein.<p><a href='/help/pathway' target='_top'>More...</a></p>Pathwayi: dTTP biosynthesis

Sites

Regions

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Biological processi

Enzyme and pathway databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Cellular componenti

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Phylogenomic databases

Family and domain databases

Sequence databases

Genome annotation databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protocols and materials databases

Genome annotation databases

Phylogenomic databases

Enzyme and pathway databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Caution

Your basket is currently empty. ⁱ

Functionⁱ

Pathwayⁱ: dTTP biosynthesis

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

GO - Cellular componentⁱ

Interactionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ