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Protein

Amidophosphoribosyltransferase

Gene

purF

Organism
Lactococcus lactis subsp. lactis (strain KF147)
Status
Unreviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the formation of phosphoribosylamine from phosphoribosylpyrophosphate (PRPP) and glutamine.UniRule annotation

Catalytic activityi

5-phospho-beta-D-ribosylamine + diphosphate + L-glutamate = L-glutamine + 5-phospho-alpha-D-ribose 1-diphosphate + H2O.UniRule annotation

Cofactori

Mg2+UniRule annotationNote: Binds 1 Mg2+ ion per subunit.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei39 – 391For GATase activityUniRule annotation
Active sitei39 – 391NucleophileUniRule annotation
Metal bindingi322 – 3221MagnesiumUniRule annotation
Metal bindingi384 – 3841MagnesiumUniRule annotation
Metal bindingi385 – 3851MagnesiumUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

GlycosyltransferaseUniRule annotation, Transferase

Keywords - Biological processi

Purine biosynthesisUniRule annotation

Keywords - Ligandi

MagnesiumUniRule annotation, Metal-bindingUniRule annotation

Enzyme and pathway databases

BioCyciLLAC684738:GI3F-1696-MONOMER.
UniPathwayiUPA00074; UER00124.

Protein family/group databases

MEROPSiC44.A01.

Names & Taxonomyi

Protein namesi
Recommended name:
AmidophosphoribosyltransferaseUniRule annotation (EC:2.4.2.14UniRule annotation)
Short name:
ATaseUniRule annotation
Alternative name(s):
Glutamine phosphoribosylpyrophosphate amidotransferaseUniRule annotation
Gene namesi
Name:purFUniRule annotationImported
Ordered Locus Names:LLKF_1649Imported
OrganismiLactococcus lactis subsp. lactis (strain KF147)Imported
Taxonomic identifieri684738 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeLactococcus
ProteomesiUP000001886 Componenti: Chromosome

Structurei

3D structure databases

ProteinModelPortaliD2BKG6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini39 – 259221Glutamine amidotransferase type-2UniRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 glutamine amidotransferase type-2 domain.UniRule annotation
In the C-terminal section; belongs to the purine/pyrimidine phosphoribosyltransferase family.UniRule annotation

Keywords - Domaini

Glutamine amidotransferaseUniRule annotation

Phylogenomic databases

HOGENOMiHOG000033688.
KOiK00764.
OMAiIAGVNVH.
OrthoDBiEOG6KT2Q1.

Family and domain databases

Gene3Di3.40.50.2020. 1 hit.
3.60.20.10. 1 hit.
HAMAPiMF_01931. PurF.
InterProiIPR017932. GATase_2_dom.
IPR029055. Ntn_hydrolases_N.
IPR000836. PRibTrfase_dom.
IPR029057. PRTase-like.
IPR005854. PurF.
[Graphical view]
PfamiPF00156. Pribosyltran. 1 hit.
[Graphical view]
PIRSFiPIRSF000485. Amd_phspho_trans. 1 hit.
SUPFAMiSSF53271. SSF53271. 1 hit.
SSF56235. SSF56235. 1 hit.
TIGRFAMsiTIGR01134. purF. 1 hit.
PROSITEiPS51278. GATASE_TYPE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

D2BKG6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFGKEQTLDL RVLGKNSSNE NSWWQQHPDD ERSHLNEECG LFGVWGHPDA
60 70 80 90 100
ARLTYFGLHA LQHRGQEGAG ILVNNNGKLN RHRGLGLVTE VFRDEKDLEE
110 120 130 140 150
LTGSSAIGHV RYATAGSANI NNIQPFQFEF HDGALGLAHN GNLTNAQSLR
160 170 180 190 200
CELEKSGAIF SSNSDTEILM HLIRRSHHPE FMGRVKEALN TVKGGFAYLI
210 220 230 240 250
MTENSIVAAL DPNGFRPLSI GKMSNGALVV ASETCAFDVV GATWIQDVQP
260 270 280 290 300
GEIIEINDVG IHVDQFTDST NMTICSMEYI YFARPDSNIA GVNVHTARKR
310 320 330 340 350
SGKILAQEAQ IDADIVIGVP NSSLSAASGY AEESGLPYEM GLIKNQYVAR
360 370 380 390 400
TFIQPTQELR EQGVRMKLSA VRGVVEGKRV IMVDDSIVRG TTSRRIVKLL
410 420 430 440 450
KDAGAAEVHV AIASPALKYP CFYGIDIQDR DELIAATHTT DEIRKAIGAD
460 470 480 490
SLTYLSQSGL VEAIGHDKLC LSYFDGEYPT PLYDYEADYL ESLAKNSVK
Length:499
Mass (Da):54,774
Last modified:February 9, 2010 - v1
Checksum:i8CBAA401909E7722
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001834 Genomic DNA. Translation: ADA65272.1.
RefSeqiWP_012898143.1. NC_013656.1.
YP_003354072.1. NC_013656.1.

Genome annotation databases

EnsemblBacteriaiADA65272; ADA65272; LLKF_1649.
KEGGillk:LLKF_1649.
PATRICi32251797. VBILacLac141273_1666.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001834 Genomic DNA. Translation: ADA65272.1.
RefSeqiWP_012898143.1. NC_013656.1.
YP_003354072.1. NC_013656.1.

3D structure databases

ProteinModelPortaliD2BKG6.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

MEROPSiC44.A01.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiADA65272; ADA65272; LLKF_1649.
KEGGillk:LLKF_1649.
PATRICi32251797. VBILacLac141273_1666.

Phylogenomic databases

HOGENOMiHOG000033688.
KOiK00764.
OMAiIAGVNVH.
OrthoDBiEOG6KT2Q1.

Enzyme and pathway databases

UniPathwayiUPA00074; UER00124.
BioCyciLLAC684738:GI3F-1696-MONOMER.

Family and domain databases

Gene3Di3.40.50.2020. 1 hit.
3.60.20.10. 1 hit.
HAMAPiMF_01931. PurF.
InterProiIPR017932. GATase_2_dom.
IPR029055. Ntn_hydrolases_N.
IPR000836. PRibTrfase_dom.
IPR029057. PRTase-like.
IPR005854. PurF.
[Graphical view]
PfamiPF00156. Pribosyltran. 1 hit.
[Graphical view]
PIRSFiPIRSF000485. Amd_phspho_trans. 1 hit.
SUPFAMiSSF53271. SSF53271. 1 hit.
SSF56235. SSF56235. 1 hit.
TIGRFAMsiTIGR01134. purF. 1 hit.
PROSITEiPS51278. GATASE_TYPE_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Genome-scale genotype-phenotype matching of two Lactococcus lactis isolates from plants identifies mechanisms of adaptation to the plant niche."
    Siezen R.J., Starrenburg M.J., Boekhorst J., Renckens B., Molenaar D., van Hylckama Vlieg J.E.
    Appl. Environ. Microbiol. 74:424-436(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: KF147Imported.

Entry informationi

Entry nameiD2BKG6_LACLK
AccessioniPrimary (citable) accession number: D2BKG6
Entry historyi
Integrated into UniProtKB/TrEMBL: February 9, 2010
Last sequence update: February 9, 2010
Last modified: May 27, 2015
This is version 44 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteomeImported

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.