D2B6P9 (D2B6P9_STRRD) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
DNA polymerase IV HAMAP-Rule MF_01113
Short name=Pol IV HAMAP-Rule MF_01113
EC=2.7.7.7 HAMAP-Rule MF_01113

Gene names

Name:	dinB HAMAP-Rule MF_01113
Ordered Locus Names:	Sros_2856 EMBL ACZ85813.1

Organism

Streptosporangium roseum (strain ATCC 12428 / DSM 43021 / JCM 3005 / NI 9100) [Complete proteome] [HAMAP] EMBL ACZ85813.1

Taxonomic identifier

479432 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Actinobacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Streptosporangineae › Streptosporangiaceae › Streptosporangium › Streptosporangium roseum

Protein attributes

Sequence length	406 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Poorly processive, error-prone DNA polymerase involved in untargeted mutagenesis. Copies undamaged DNA at stalled replication forks, which arise in vivo from mismatched or misaligned primer ends. These misaligned primers can be extended by PolIV. Exhibits no 3'-5' exonuclease (proofreading) activity. May be involved in translesional synthesis, in conjunction with the beta clamp from PolIII By similarity. HAMAP-Rule MF_01113
Catalytic activity	Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1). HAMAP-Rule MF_01113 SAAS SAAS024728
Cofactor	Binds 2 magnesium ions per subunit By similarity. HAMAP-Rule MF_01113
Subunit structure	Monomer By similarity. HAMAP-Rule MF_01113
Subcellular location	Cytoplasm By similarity HAMAP-Rule MF_01113.
Sequence similarities	Belongs to the DNA polymerase type-Y family. HAMAP-Rule MF_01113 Contains 1 umuC domain. HAMAP-Rule MF_01113 SAAS SAAS024728

Ontologies

Keywords
Biological process	DNA damage DNA repair RuleBase RU003987 SAAS SAAS024728 HAMAP-Rule MF_01113 DNA replication SAAS SAAS024728 HAMAP-Rule MF_01113
Cellular component	Cytoplasm SAAS SAAS017961 HAMAP-Rule MF_01113
Ligand	DNA-binding SAAS SAAS024728 HAMAP-Rule MF_01113 Magnesium SAAS SAAS024728 HAMAP-Rule MF_01113 Metal-binding SAAS SAAS024728 HAMAP-Rule MF_01113
Molecular function	DNA-directed DNA polymerase SAAS SAAS024728 HAMAP-Rule MF_01113 EMBL ACZ85813.1 Mutator protein HAMAP-Rule MF_01113 Nucleotidyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	DNA repair Inferred from electronic annotation. Source: HAMAP DNA-dependent DNA replication Inferred from electronic annotation. Source: HAMAP
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	DNA-directed DNA polymerase activity Inferred from electronic annotation. Source: HAMAP damaged DNA binding Inferred from electronic annotation. Source: InterPro magnesium ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Regions
	Domain	17 – 197	181	UmuC By similarity HAMAP-Rule MF_01113
Sites
	Active site	116	1	By similarity HAMAP-Rule MF_01113
	Metal binding	21	1	Magnesium By similarity HAMAP-Rule MF_01113
	Metal binding	115	1	Magnesium By similarity HAMAP-Rule MF_01113
	Site	26	1	Substrate discrimination By similarity HAMAP-Rule MF_01113

Sequences

	Sequence		Length	Mass (Da)
	D2B6P9 [UniParc]. Last modified February 9, 2010. Version 1. Checksum: 1C7DB6439C0935BA	FASTA	406	43,698
	10 20 30 40 50 60 MPRPGSDLGA DDTGCPILHV DMDAFYASVE LRERPELRGT PVVIGAPGAR GVVLSATYEA 70 80 90 100 110 120 RRFGVHSAMP MTRARRLCPQ AVIIPPSRGK YAEVSRGVME IFHTFTPEVE PIASDEAFLD 130 140 150 160 170 180 VGGARKRLGP PAAIAAMIRE QVVRKHGITC SVGVANSKFV AKLASQHCKP DGLLVVPAGR 190 200 210 220 230 240 VVDFLHPLPV GALWGVGERT EQSLVRLGIR TVGDLAGVPV ATLQRQLGQA AGAHLAALAW 250 260 270 280 290 300 GRDERPVTPH APDKSIGAEE TFATDIGDPV KIRVELLRLS ERVAARLREG GHVGRTVSVK 310 320 330 340 350 360 LRRADFSTIS RSRTLREPTD VAQTLYATAC DLYAAAGLDR DRLRLVGVRV ENLSPAGEAT 370 380 390 400 RQLGLGERET GWREAEQAMD RAARRFGRDV VLPASLVRPP FDGMAP

References

[1]

"Complete genome sequence of Streptosporangium roseum type strain (NI 9100T)." Nolan M., Sikorski J., Jando M., Lucas S., Lapidus A., Glavina Del Rio T., Chen F., Tice H., Pitluck S., Cheng J.F., Chertkov O., Sims D., Meincke L., Brettin T., Han C., Detter J.C., Bruce D., Goodwin L. , Land M., Hauser L., Chang Y.J., Jeffries C.D., Ivanova N., Mavromatis K., Mikhailova N., Chen A., Palaniappan K., Chain P., Rohde M., Goker M., Bristow J., Eisen J.A., Markowitz V., Hugenholtz P., Kyrpides N.C., Klenk H.P. Stand. Genomic Sci. 2:29-37(2010) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 12428 / DSM 43021 / JCM 3005 / NI 9100.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001814 Genomic DNA. Translation: ACZ85813.1.
RefSeq	YP_003338556.1. NC_013595.1.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ACZ85813; ACZ85813; Sros_2856.
GeneID	8666142.
KEGG	sro:Sros_2856.
PATRIC	32467683. VBIStrRos112010_2835.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HOG000082707.
KO	K02346.
OMA	SAMSSRH.
Family and domain databases
Gene3D	3.30.1490.100. 1 hit.
HAMAP	MF_01113. DNApol_IV.
InterPro	IPR017961. DNA_pol_Y-fam_little_finger. IPR001126. DNA_repair_prot_UmuC-like. IPR017963. DNA_repair_prot_UmuC-like_N. IPR022880. DNApol_IV. IPR024728. PolY_HhH_motif. [Graphical view]
Pfam	PF00817. IMS. 1 hit. PF11799. IMS_C. 1 hit. PF11798. IMS_HHH. 1 hit. [Graphical view]
SUPFAM	SSF100879. DNA_pol_Y-fam_little_finger. 1 hit.
PROSITE	PS50173. UMUC. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

D2B6P9_STRRD

Accession

Primary (citable) accession number: D2B6P9

Entry history

Integrated into UniProtKB/TrEMBL:	February 9, 2010
Last sequence update:	February 9, 2010
Last modified:	May 1, 2013
This is version 28 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)