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Protein

DNA polymerase IV

Gene

dinB

Organism
Streptosporangium roseum (strain ATCC 12428 / DSM 43021 / JCM 3005 / NI 9100)
Status
Unreviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Poorly processive, error-prone DNA polymerase involved in untargeted mutagenesis. Copies undamaged DNA at stalled replication forks, which arise in vivo from mismatched or misaligned primer ends. These misaligned primers can be extended by PolIV. Exhibits no 3'-5' exonuclease (proofreading) activity. May be involved in translesional synthesis, in conjunction with the beta clamp from PolIII.UniRule annotation

Catalytic activityi

Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).UniRule annotationSAAS annotation

Cofactori

Mg2+UniRule annotationSAAS annotationNote: Binds 2 magnesium ions per subunit.UniRule annotationSAAS annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi21 – 211MagnesiumUniRule annotation
Sitei26 – 261Substrate discriminationUniRule annotation
Metal bindingi115 – 1151MagnesiumUniRule annotation
Active sitei116 – 1161UniRule annotation

GO - Molecular functioni

  1. damaged DNA binding Source: InterPro
  2. DNA-directed DNA polymerase activity Source: UniProtKB-HAMAP
  3. magnesium ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. DNA-dependent DNA replication Source: UniProtKB-HAMAP
  2. DNA repair Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

DNA-directed DNA polymeraseUniRule annotationSAAS annotation, Mutator proteinUniRule annotation, Nucleotidyltransferase, Transferase

Keywords - Biological processi

DNA damage, DNA repairUniRule annotation, DNA replicationUniRule annotationSAAS annotation

Keywords - Ligandi

DNA-bindingUniRule annotationSAAS annotation, MagnesiumUniRule annotationSAAS annotation, Metal-bindingUniRule annotationSAAS annotation

Enzyme and pathway databases

BioCyciSROS479432:GI0V-2878-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA polymerase IVUniRule annotation (EC:2.7.7.7UniRule annotation)
Short name:
Pol IVUniRule annotation
Gene namesi
Name:dinBUniRule annotation
Ordered Locus Names:Sros_2856Imported
OrganismiStreptosporangium roseum (strain ATCC 12428 / DSM 43021 / JCM 3005 / NI 9100)Imported
Taxonomic identifieri479432 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesStreptosporangineaeStreptosporangiaceaeStreptosporangium
ProteomesiUP000002029 Componenti: Chromosome

Subcellular locationi

Cytoplasm UniRule annotationSAAS annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

CytoplasmUniRule annotationSAAS annotation

Interactioni

Subunit structurei

Monomer.UniRule annotationSAAS annotation

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini17 – 197181UmuCUniRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the DNA polymerase type-Y family.UniRule annotation
Contains 1 umuC domain.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000082707.
KOiK02346.
OMAiLWIFANG.
OrthoDBiEOG6FZ4D8.

Family and domain databases

Gene3Di3.30.1490.100. 1 hit.
HAMAPiMF_01113. DNApol_IV.
InterProiIPR017961. DNA_pol_Y-fam_little_finger.
IPR001126. DNA_repair_prot_UmuC-like.
IPR017963. DNA_repair_prot_UmuC-like_N.
IPR022880. DNApol_IV.
IPR024728. PolY_HhH_motif.
[Graphical view]
PfamiPF00817. IMS. 1 hit.
PF11799. IMS_C. 1 hit.
PF11798. IMS_HHH. 1 hit.
[Graphical view]
SUPFAMiSSF100879. SSF100879. 1 hit.
PROSITEiPS50173. UMUC. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

D2B6P9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPRPGSDLGA DDTGCPILHV DMDAFYASVE LRERPELRGT PVVIGAPGAR
60 70 80 90 100
GVVLSATYEA RRFGVHSAMP MTRARRLCPQ AVIIPPSRGK YAEVSRGVME
110 120 130 140 150
IFHTFTPEVE PIASDEAFLD VGGARKRLGP PAAIAAMIRE QVVRKHGITC
160 170 180 190 200
SVGVANSKFV AKLASQHCKP DGLLVVPAGR VVDFLHPLPV GALWGVGERT
210 220 230 240 250
EQSLVRLGIR TVGDLAGVPV ATLQRQLGQA AGAHLAALAW GRDERPVTPH
260 270 280 290 300
APDKSIGAEE TFATDIGDPV KIRVELLRLS ERVAARLREG GHVGRTVSVK
310 320 330 340 350
LRRADFSTIS RSRTLREPTD VAQTLYATAC DLYAAAGLDR DRLRLVGVRV
360 370 380 390 400
ENLSPAGEAT RQLGLGERET GWREAEQAMD RAARRFGRDV VLPASLVRPP

FDGMAP
Length:406
Mass (Da):43,698
Last modified:February 9, 2010 - v1
Checksum:i1C7DB6439C0935BA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001814 Genomic DNA. Translation: ACZ85813.1.
RefSeqiYP_003338556.1. NC_013595.1.

Genome annotation databases

EnsemblBacteriaiACZ85813; ACZ85813; Sros_2856.
GeneIDi8666142.
KEGGisro:Sros_2856.
PATRICi32467683. VBIStrRos112010_2835.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001814 Genomic DNA. Translation: ACZ85813.1.
RefSeqiYP_003338556.1. NC_013595.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACZ85813; ACZ85813; Sros_2856.
GeneIDi8666142.
KEGGisro:Sros_2856.
PATRICi32467683. VBIStrRos112010_2835.

Phylogenomic databases

HOGENOMiHOG000082707.
KOiK02346.
OMAiLWIFANG.
OrthoDBiEOG6FZ4D8.

Enzyme and pathway databases

BioCyciSROS479432:GI0V-2878-MONOMER.

Family and domain databases

Gene3Di3.30.1490.100. 1 hit.
HAMAPiMF_01113. DNApol_IV.
InterProiIPR017961. DNA_pol_Y-fam_little_finger.
IPR001126. DNA_repair_prot_UmuC-like.
IPR017963. DNA_repair_prot_UmuC-like_N.
IPR022880. DNApol_IV.
IPR024728. PolY_HhH_motif.
[Graphical view]
PfamiPF00817. IMS. 1 hit.
PF11799. IMS_C. 1 hit.
PF11798. IMS_HHH. 1 hit.
[Graphical view]
SUPFAMiSSF100879. SSF100879. 1 hit.
PROSITEiPS50173. UMUC. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 12428 / DSM 43021 / JCM 3005 / NI 9100Imported.

Entry informationi

Entry nameiD2B6P9_STRRD
AccessioniPrimary (citable) accession number: D2B6P9
Entry historyi
Integrated into UniProtKB/TrEMBL: February 9, 2010
Last sequence update: February 9, 2010
Last modified: March 4, 2015
This is version 40 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteomeImported

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.