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D2B4S0

- D2B4S0_STRRD

UniProt

D2B4S0 - D2B4S0_STRRD

Protein

Lipoyl synthase

Gene

lipA

Organism
Streptosporangium roseum (strain ATCC 12428 / DSM 43021 / JCM 3005 / NI 9100)
Status
Unreviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 39 (01 Oct 2014)
      Sequence version 1 (09 Feb 2010)
      Previous versions | rss
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    Functioni

    Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives.UniRule annotation

    Catalytic activityi

    Protein N(6)-(octanoyl)lysine + 2 sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = protein N(6)-(lipoyl)lysine + 2 (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.UniRule annotation

    Cofactori

    Binds 2 4Fe-4S clusters per subunit. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi55 – 551Iron-sulfur 1 (4Fe-4S)UniRule annotation
    Metal bindingi60 – 601Iron-sulfur 1 (4Fe-4S)UniRule annotation
    Metal bindingi66 – 661Iron-sulfur 1 (4Fe-4S)UniRule annotation
    Metal bindingi81 – 811Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation
    Metal bindingi85 – 851Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation
    Metal bindingi88 – 881Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation

    GO - Molecular functioni

    1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-HAMAP
    2. lipoate synthase activity Source: UniProtKB-HAMAP
    3. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. protein lipoylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    TransferaseUniRule annotationImported

    Keywords - Ligandi

    4Fe-4SUniRule annotation, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionineUniRule annotation

    Enzyme and pathway databases

    BioCyciSROS479432:GI0V-2662-MONOMER.
    UniPathwayiUPA00538; UER00593.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Lipoyl synthaseUniRule annotation (EC:2.8.1.8UniRule annotation)
    Alternative name(s):
    Lip-synUniRule annotation
    Lipoate synthaseUniRule annotation
    Lipoic acid synthaseUniRule annotation
    Sulfur insertion protein LipAUniRule annotation
    Gene namesi
    Name:lipAUniRule annotation
    Ordered Locus Names:Sros_2640Imported
    OrganismiStreptosporangium roseum (strain ATCC 12428 / DSM 43021 / JCM 3005 / NI 9100)Imported
    Taxonomic identifieri479432 [NCBI]
    Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesStreptosporangineaeStreptosporangiaceaeStreptosporangium
    ProteomesiUP000002029: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    CytoplasmUniRule annotation

    Structurei

    3D structure databases

    ProteinModelPortaliD2B4S0.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the radical SAM superfamily. Lipoyl synthase family.UniRule annotation

    Phylogenomic databases

    HOGENOMiHOG000235997.
    KOiK03644.
    OMAiEWLRRPI.
    OrthoDBiEOG6038ZS.

    Family and domain databases

    Gene3Di3.20.20.70. 1 hit.
    HAMAPiMF_00206. Lipoyl_synth.
    InterProiIPR013785. Aldolase_TIM.
    IPR006638. Elp3/MiaB/NifB.
    IPR003698. Lipoyl_synth.
    IPR007197. rSAM.
    [Graphical view]
    PANTHERiPTHR10949. PTHR10949. 1 hit.
    PfamiPF04055. Radical_SAM. 1 hit.
    [Graphical view]
    PIRSFiPIRSF005963. Lipoyl_synth. 1 hit.
    SMARTiSM00729. Elp3. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR00510. lipA. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    D2B4S0-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTVAPEGRKL LRLEVRNAET PIEKKPEWIK TKFRTGPNHT ELRALVKREG    50
    LHTVCQEAGC PNISECWEDR EATFLIGGDQ CTRRCDFCQI DTGKPKEYDT 100
    DEPRRVAESV RQMGLNYATV TGVARDDLPD GGAWLYAETA RQIHALLPGC 150
    GVELLVPDFN GDQGQLEEVF SAAPEVFAHN VETVPRIFKR IRPGFRYERS 200
    LGVITKAREA GLVTKSNLIL GMGEDREEVV QAMRDLHEAG CDLLTVTQYL 250
    RPTPRHHPVD RWVKPEEFVE LQKEAEAIGF AGVLSGPLVR SSYRAGRLYK 300
    QAIEARQHA 309
    Length:309
    Mass (Da):34,775
    Last modified:February 9, 2010 - v1
    Checksum:i63909FAC07A9B8C0
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP001814 Genomic DNA. Translation: ACZ85606.1.
    RefSeqiWP_012889351.1. NC_013595.1.
    YP_003338349.1. NC_013595.1.

    Genome annotation databases

    EnsemblBacteriaiACZ85606; ACZ85606; Sros_2640.
    GeneIDi8665926.
    KEGGisro:Sros_2640.
    PATRICi32467249. VBIStrRos112010_2618.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP001814 Genomic DNA. Translation: ACZ85606.1 .
    RefSeqi WP_012889351.1. NC_013595.1.
    YP_003338349.1. NC_013595.1.

    3D structure databases

    ProteinModelPortali D2B4S0.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ACZ85606 ; ACZ85606 ; Sros_2640 .
    GeneIDi 8665926.
    KEGGi sro:Sros_2640.
    PATRICi 32467249. VBIStrRos112010_2618.

    Phylogenomic databases

    HOGENOMi HOG000235997.
    KOi K03644.
    OMAi EWLRRPI.
    OrthoDBi EOG6038ZS.

    Enzyme and pathway databases

    UniPathwayi UPA00538 ; UER00593 .
    BioCyci SROS479432:GI0V-2662-MONOMER.

    Family and domain databases

    Gene3Di 3.20.20.70. 1 hit.
    HAMAPi MF_00206. Lipoyl_synth.
    InterProi IPR013785. Aldolase_TIM.
    IPR006638. Elp3/MiaB/NifB.
    IPR003698. Lipoyl_synth.
    IPR007197. rSAM.
    [Graphical view ]
    PANTHERi PTHR10949. PTHR10949. 1 hit.
    Pfami PF04055. Radical_SAM. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF005963. Lipoyl_synth. 1 hit.
    SMARTi SM00729. Elp3. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR00510. lipA. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 12428 / DSM 43021 / JCM 3005 / NI 9100Imported.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 12428 / DSM 43021 / JCM 3005 / NI 9100Imported.

    Entry informationi

    Entry nameiD2B4S0_STRRD
    AccessioniPrimary (citable) accession number: D2B4S0
    Entry historyi
    Integrated into UniProtKB/TrEMBL: February 9, 2010
    Last sequence update: February 9, 2010
    Last modified: October 1, 2014
    This is version 39 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiUnreviewed (UniProtKB/TrEMBL)

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteomeImported

    External Data

    Dasty 3