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D2B4S0 (D2B4S0_STRRD) Unreviewed, UniProtKB/TrEMBL

Last modified June 11, 2014. Version 37. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry infoCustomize order

Names and origin

Protein namesRecommended name:
Lipoyl synthase HAMAP-Rule MF_00206

EC=2.8.1.8 HAMAP-Rule MF_00206
Alternative name(s):
Lip-syn HAMAP-Rule MF_00206
Lipoate synthase HAMAP-Rule MF_00206
Lipoic acid synthase HAMAP-Rule MF_00206
Sulfur insertion protein LipA HAMAP-Rule MF_00206
Gene names
Name:lipA HAMAP-Rule MF_00206
Ordered Locus Names:Sros_2640 EMBL ACZ85606.1
OrganismStreptosporangium roseum (strain ATCC 12428 / DSM 43021 / JCM 3005 / NI 9100) [Complete proteome] [HAMAP] EMBL ACZ85606.1
Taxonomic identifier479432 [NCBI]
Taxonomic lineageBacteriaActinobacteriaActinobacteridaeActinomycetalesStreptosporangineaeStreptosporangiaceaeStreptosporangium

Protein attributes

Sequence length309 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives By similarity. HAMAP-Rule MF_00206

Catalytic activity

Protein N(6)-(octanoyl)lysine + 2 sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = protein N(6)-(lipoyl)lysine + 2 (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine. HAMAP-Rule MF_00206

Cofactor

Binds 2 4Fe-4S clusters per subunit. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity. HAMAP-Rule MF_00206

Pathway

Protein modification; protein lipoylation via endogenous pathway; protein N(6)-(lipoyl)lysine from octanoyl-[acyl-carrier-protein]: step 2/2. HAMAP-Rule MF_00206

Subcellular location

Cytoplasm By similarity SAAS SAAS003698 HAMAP-Rule MF_00206.

Sequence similarities

Belongs to the radical SAM superfamily. Lipoyl synthase family. HAMAP-Rule MF_00206

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Sites

Metal binding551Iron-sulfur 1 (4Fe-4S) By similarity HAMAP-Rule MF_00206
Metal binding601Iron-sulfur 1 (4Fe-4S) By similarity HAMAP-Rule MF_00206
Metal binding661Iron-sulfur 1 (4Fe-4S) By similarity HAMAP-Rule MF_00206
Metal binding811Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity HAMAP-Rule MF_00206
Metal binding851Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity HAMAP-Rule MF_00206
Metal binding881Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity HAMAP-Rule MF_00206

Sequences

Sequence LengthMass (Da)Tools
D2B4S0 [UniParc].

Last modified February 9, 2010. Version 1.
Checksum: 63909FAC07A9B8C0

FASTA30934,775
        10         20         30         40         50         60 
MTVAPEGRKL LRLEVRNAET PIEKKPEWIK TKFRTGPNHT ELRALVKREG LHTVCQEAGC 

        70         80         90        100        110        120 
PNISECWEDR EATFLIGGDQ CTRRCDFCQI DTGKPKEYDT DEPRRVAESV RQMGLNYATV 

       130        140        150        160        170        180 
TGVARDDLPD GGAWLYAETA RQIHALLPGC GVELLVPDFN GDQGQLEEVF SAAPEVFAHN 

       190        200        210        220        230        240 
VETVPRIFKR IRPGFRYERS LGVITKAREA GLVTKSNLIL GMGEDREEVV QAMRDLHEAG 

       250        260        270        280        290        300 
CDLLTVTQYL RPTPRHHPVD RWVKPEEFVE LQKEAEAIGF AGVLSGPLVR SSYRAGRLYK 


QAIEARQHA 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP001814 Genomic DNA. Translation: ACZ85606.1.
RefSeqYP_003338349.1. NC_013595.1.

3D structure databases

ProteinModelPortalD2B4S0.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaACZ85606; ACZ85606; Sros_2640.
GeneID8665926.
KEGGsro:Sros_2640.
PATRIC32467249. VBIStrRos112010_2618.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMHOG000235997.
KOK03644.
OMAEWLRRPI.
OrthoDBEOG6038ZS.

Enzyme and pathway databases

BioCycSROS479432:GI0V-2662-MONOMER.
UniPathwayUPA00538; UER00593.

Family and domain databases

Gene3D3.20.20.70. 1 hit.
HAMAPMF_00206. Lipoyl_synth.
InterProIPR013785. Aldolase_TIM.
IPR006638. Elp3/MiaB/NifB.
IPR003698. Lipoyl_synth.
IPR007197. rSAM.
[Graphical view]
PANTHERPTHR10949. PTHR10949. 1 hit.
PfamPF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFPIRSF005963. Lipoyl_synth. 1 hit.
SMARTSM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsTIGR00510. lipA. 1 hit.
ProtoNetSearch...

Entry information

Entry nameD2B4S0_STRRD
AccessionPrimary (citable) accession number: D2B4S0
Entry history
Integrated into UniProtKB/TrEMBL: February 9, 2010
Last sequence update: February 9, 2010
Last modified: June 11, 2014
This is version 37 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)