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D2A8Y2 (D2A8Y2_SHIF2) Unreviewed, UniProtKB/TrEMBL

Last modified May 1, 2013. Version 29. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Ribosomal RNA small subunit methyltransferase B HAMAP-Rule MF_01856
EC=2.1.1.176 HAMAP-Rule MF_01856
Alternative name(s):
16S rRNA m5C967 methyltransferase HAMAP-Rule MF_01856
rRNA (cytosine-C(5)-)-methyltransferase RsmB HAMAP-Rule MF_01856
Gene names
Name:rsmB HAMAP-Rule MF_01856 EMBL ADA75641.1
Synonyms:sun HAMAP-Rule MF_01856
Ordered Locus Names:SFxv_3636 EMBL ADA75641.1
OrganismShigella flexneri serotype X (strain 2002017) [Complete proteome] [HAMAP] EMBL ADA75641.1
Taxonomic identifier591020 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Shigella ›

Protein attributes

Sequence length436 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Specifically methylates the cytosine at position 967 (m5C967) of 16S rRNA By similarity. HAMAP-Rule MF_01856 SAAS SAAS023267

Catalytic activity

S-adenosyl-L-methionine + cytosine(967) in 16S rRNA = S-adenosyl-L-homocysteine + 5-methylcytosine(967) in 16S rRNA. HAMAP-Rule MF_01856 SAAS SAAS023267

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_01856 SAAS SAAS023267.

Sequence similarities

Belongs to the methyltransferase superfamily. RsmB/NOP family. HAMAP-Rule MF_01856

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Regions

Region261 – 2677S-adenosyl-L-methionine binding By similarity HAMAP-Rule MF_01856

Sites

Active site3821Nucleophile By similarity HAMAP-Rule MF_01856
Binding site2841S-adenosyl-L-methionine By similarity HAMAP-Rule MF_01856
Binding site3101S-adenosyl-L-methionine By similarity HAMAP-Rule MF_01856
Binding site3291S-adenosyl-L-methionine By similarity HAMAP-Rule MF_01856

Sequences

Sequence LengthMass (Da)Tools
D2A8Y2 [UniParc].

Last modified February 9, 2010. Version 1.
Checksum: 9091D419A5722F74

FASTA43649,264
        10         20         30         40         50         60 
MPSIFILMKK QRNLRSMAAQ AVEQVVEQGQ SLSNILPPLQ QKVSDKDKAL LQELCFGVLR 

        70         80         90        100        110        120 
TLSQLDWLIN KLMARPMTGK QRTVHYLIMV GLYQLLYTRI PPHAALAETV EGAIAIKRPQ 

       130        140        150        160        170        180 
LKGLINGVLR QFQRRQEELL AEFNTRDARY LHPSWLLKRL QKAYPEQWQS IAEANNQRPP 

       190        200        210        220        230        240 
MWLRINRTHH SRDSWLALLD EAGMKGFPHA DYPDAVRLET PAPVHALPGF EDGWVTVQDA 

       250        260        270        280        290        300 
SAQGCMTWLA PQNGEHILDL CAAPGGKTTH ILEVAPEAQV VAVDIDEQRL SRIYDNLKRL 

       310        320        330        340        350        360 
GMKATVKQGD GRYPSQWCGE QQFDRILLDA PCSATGVIRR HPDIKWLRRD RDIPELAQLQ 

       370        380        390        400        410        420 
SEILDAIWPH LKSGGTLVYA TCSVLPEENS LQIKAFLQRT ADAELCETGT PEQPGKQNQP 

       430 
GAEEGDGFFY AKLIKK

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References

[1]"Emergence of a new multidrug resistance serotype X variant in an epidemic clone of Shigella flexneri."
Ye C., Lan R., Xia S., Zhang J., Sun Q., Zhang S., Jing H., Wang L., Li Z., Zhou Z., Zhao A., Cui Z., Cao J., Jin D., Huang L., Wang Y., Luo X., Bai X. expand/collapse author list , Wang Y., Wang P., Xu Q., Xu J.
J. Clin. Microbiol. 48:419-426(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 2002017 EMBL ADA75641.1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP001383 Genomic DNA. Translation: ADA75641.1.
RefSeqYP_005728934.1. NC_017328.1.

3D structure databases

ProteinModelPortalD2A8Y2.
ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaADA75641; ADA75641; SFxv_3636.
GeneID12319499.
KEGGsfe:SFxv_3636.
PATRIC36749255. VBIShiFle60233_3910.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMHOG000037300.
KOK03500.

Family and domain databases

Gene3D1.10.940.10. 1 hit.
HAMAPMF_01856. 16SrRNA_methyltr_B.
InterProIPR001678. Fmu/NOL1/Nop2p.
IPR018314. Fmu/NOL1/Nop2p_CS.
IPR006027. NusB_RsmB_TIM44.
IPR023267. RCMT.
IPR004573. rRNA_ssu_MeTfrase_B.
IPR023541. rRNA_ssu_MeTfrase_B_ent.
[Graphical view]
PfamPF01189. Nol1_Nop2_Fmu. 1 hit.
PF01029. NusB. 1 hit.
[Graphical view]
PRINTSPR02008. RCMTFAMILY.
SUPFAMSSF48013. NusB_RsmB_TIM44. 1 hit.
TIGRFAMsTIGR00563. rsmB. 1 hit.
PROSITEPS01153. NOL1_NOP2_SUN. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameD2A8Y2_SHIF2
AccessionPrimary (citable) accession number: D2A8Y2
Entry history
Integrated into UniProtKB/TrEMBL: February 9, 2010
Last sequence update: February 9, 2010
Last modified: May 1, 2013
This is version 29 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info