D1ZBF6 (AMPP3_SORMK) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable Xaa-Pro aminopeptidase PEPP
EC=3.4.11.9

Alternative name(s):
Aminoacylproline aminopeptidase
Prolidase

Gene names

Name:	PEPP
ORF Names:	SMAC_02260

Organism

Sordaria macrospora (strain ATCC MYA-333 / DSM 997 / K(L3346) / K-hell) [Complete proteome]

Taxonomic identifier

771870 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Pezizomycotina › leotiomyceta › sordariomyceta › Sordariomycetes › Sordariomycetidae › Sordariales › Sordariaceae › Sordaria › Sordaria macrospora

Protein attributes

Sequence length	591 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the removal of a penultimate prolyl residue from the N-termini of peptides By similarity.
Catalytic activity	Release of any N-terminal amino acid, including proline, that is linked to proline, even from a dipeptide or tripeptide.
Cofactor	Binds 2 manganese ions per subunit By similarity.
Sequence similarities	Belongs to the peptidase M24B family.

Ontologies

Keywords
Ligand	Manganese Metal-binding
Molecular function	Aminopeptidase Hydrolase Metalloprotease Protease
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	cellular process Inferred from electronic annotation. Source: InterPro proteolysis Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	aminopeptidase activity Inferred from electronic annotation. Source: UniProtKB-KW manganese ion binding Inferred from electronic annotation. Source: InterPro metallopeptidase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 591	591	Probable Xaa-Pro aminopeptidase PEPP		PRO_0000411889
Sites
	Metal binding	322	1	Manganese 2 By similarity
	Metal binding	333	1	Manganese 1 By similarity
	Metal binding	333	1	Manganese 2 By similarity
	Metal binding	511	1	Manganese 1 By similarity
	Metal binding	552	1	Manganese 1 By similarity
	Metal binding	552	1	Manganese 2 By similarity

Sequences

	Sequence		Length	Mass (Da)
	D1ZBF6 [UniParc]. Last modified February 9, 2010. Version 1. Checksum: 3E0D85D67C9EFA35	FASTA	591	66,317
	10 20 30 40 50 60 MANSDEIHYD LVEDDEFDIV NIVVEGLDET SIHSPPPSVS AATHGGVKNP SFSQRRTSGR 70 80 90 100 110 120 HDFQKWLHEE LGKYPAKTHA QKVVEELDAP KSGLIYLRGF NEPLYPYSDQ GPPFRQQRHF 130 140 150 160 170 180 FYLSGADFPG CAVTYDIPRQ HLIVWIRRND PRLSLWYGTT PSIEEVKSKS DVSDVRYIDD 190 200 210 220 230 240 VTKYLHANLT PDTTLFLLHP DQTPKLEPPS LNLRHRFKGP KIDTTSLLPA IEAARVIKTP 250 260 270 280 290 300 HEISLIRRAV ALTSLAHRMV LQRIKHLSNE REAHAVFEGF CISQGAPRQS YAVIAASGAN 310 320 330 340 350 360 ASTLHYEAND QPLEGKQTML LDAGCEWGCY ASDVTRTFPL KGKWTKEGEE IYKVVERMQR 370 380 390 400 410 420 ETIDAIRPGR LYYKLHLVAC LVAVEELMKL GILHNGTRTE ILAKGTVAAF FPHGLGHHVG 430 440 450 460 470 480 LEVHDVSGRE RLLLSSSSSS GLSRQAISGS RRLPPPRNMK REQVTPEDLA AMYREAMMDT 490 500 510 520 530 540 AGDEVEQSAV APPPPYRGRQ RLKENMVVTV EPGIYFHRPY IQSFFLSNPD HAKYINTKVL 550 560 570 580 590 DRYWDVGGVR IEDCILVTKD GYENLTTAPK GKEALKIINA GIPGFPCDGK A

References

[1]

"De novo assembly of a 40 Mb eukaryotic genome from short sequence reads: Sordaria macrospora, a model organism for fungal morphogenesis." Nowrousian M., Stajich J.E., Chu M., Engh I., Espagne E., Halliday K., Kamerewerd J., Kempken F., Knab B., Kuo H.-C., Osiewacz H.D., Poeggeler S., Read N.D., Seiler S., Smith K.M., Zickler D., Kueck U., Freitag M. PLoS Genet. 6:E1000891-E1000891(2010) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC MYA-333 / DSM 997 / K(L3346) / K-hell.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CABT02000023 Genomic DNA. Translation: CCC12038.1.
RefSeq	XP_003350547.1. XM_003350499.1.
3D structure databases
ProteinModelPortal	D1ZBF6.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	10808095.
KEGG	smp:SMAC_02260.
Phylogenomic databases
KO	K14213.
Family and domain databases
Gene3D	3.90.230.10. 2 hits.
InterPro	IPR007865. Aminopep_P_N. IPR000994. Pept_M24_structural-domain. IPR001131. Peptidase_M24B_aminopep-P_CS. [Graphical view]
Pfam	PF05195. AMP_N. 1 hit. PF00557. Peptidase_M24. 1 hit. [Graphical view]
SMART	SM01011. AMP_N. 1 hit. [Graphical view]
SUPFAM	SSF55920. Peptidase_M24_cat_core. 1 hit.
PROSITE	PS00491. PROLINE_PEPTIDASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AMPP3_SORMK

Accession

Primary (citable) accession number: D1ZBF6
Secondary accession number(s): F7W336

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 27, 2011
Last sequence update:	February 9, 2010
Last modified:	March 6, 2013
This is version 24 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program