D1Z9B4 (DAPB_SORMK) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable dipeptidyl-aminopeptidase B
Short name=DPAP B
EC=3.4.14.5

Gene names

Name:	DAPB
ORF Names:	SMAC_05958

Organism

Sordaria macrospora (strain ATCC MYA-333 / DSM 997 / K(L3346) / K-hell) [Complete proteome]

Taxonomic identifier

771870 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Pezizomycotina › leotiomyceta › sordariomyceta › Sordariomycetes › Sordariomycetidae › Sordariales › Sordariaceae › Sordaria › Sordaria macrospora

Protein attributes

Sequence length	924 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Type IV dipeptidyl-peptidase which removes N-terminal dipeptides sequentially from polypeptides having unsubstituted N-termini provided that the penultimate residue is proline By similarity.
Catalytic activity	Release of an N-terminal dipeptide, Xaa-Yaa-|-Zaa-, from a polypeptide, preferentially when Yaa is Pro, provided Zaa is neither Pro nor hydroxyproline.
Subcellular location	Vacuole membrane; Single-pass type II membrane protein By similarity. Note: Lysosome-like vacuoles By similarity.
Sequence similarities	Belongs to the peptidase S9B family.

Ontologies

Keywords
Cellular component	Membrane Vacuole
Domain	Signal-anchor Transmembrane Transmembrane helix
Molecular function	Aminopeptidase Hydrolase Protease Serine protease
PTM	Glycoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	proteolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW vacuolar membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	aminopeptidase activity Inferred from electronic annotation. Source: UniProtKB-KW serine-type endopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 924	924	Probable dipeptidyl-aminopeptidase B		PRO_0000412163
Regions
	Topological domain	1 – 111	111	Cytoplasmic Potential
	Transmembrane	112 – 132	21	Helical; Signal-anchor for type II membrane protein; Potential
	Topological domain	133 – 924	792	Vacuolar Potential
Sites
	Active site	768	1	Charge relay system By similarity
	Active site	845	1	Charge relay system By similarity
	Active site	878	1	Charge relay system By similarity
Amino acid modifications
	Glycosylation	231	1	N-linked (GlcNAc...) Potential
	Glycosylation	364	1	N-linked (GlcNAc...) Potential
	Glycosylation	827	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	D1Z9B4 [UniParc]. Last modified February 9, 2010. Version 1. Checksum: 70999F5B74E9B34C	FASTA	924	104,058
	10 20 30 40 50 60 MPPFTYSDDT LRSGRDRFRD HSPSQHRRSM SQETDSSAST TSIVFDRIQE RLDTKEFTPR 70 80 90 100 110 120 GTDGDDDGSL KDELNNDDLE TGPFLGNADS SSRSDQRSPG DGQRMDRSLR RWLFIVSGVL 130 140 150 160 170 180 VATWVIGLFV FVSSKAYKPS SSFAHDPQAT VTHGTGKKVT LDQVLNNQWR AKSHSISWIA 190 200 210 220 230 240 GANGEDGLLL EKEGVGKDYL VVEDVRAQNP SSVQASKSKA LIKEKLFEFA NKTYWPSITV 250 260 270 280 290 300 PSRDLKKVLL ATDVKNNWRH SYYAVYWIFD VETQQVEPLV PYDVEARLQL ASWSPTSDAI 310 320 330 340 350 360 VYTRDNNMFL RKLGSDKIVQ ITRDGSADVF NGVPDWVYEE EVLASGVATW WSEDGQYVAF 370 380 390 400 410 420 LRTNETGVPE YPIQYFVSRP SGEEPKPGEE NYPEVRQIKY PKAGAHNPIV DLKFYDVKRG 430 440 450 460 470 480 DVFSVDISGR FADDDRLITE VVWAGKQVLI KETNRVSDVM RVVLVDVGSR TGKAVRTVDV 490 500 510 520 530 540 NAIDGGWFEI SHKTKFIPAD PVNGRPDDGY VDTIIHDNGD HLAYFTPLDN PEPIMLTSGD 550 560 570 580 590 600 YEVVDAPSAV DLQRNLVYFV STKESSIQRH VYQVKLTGED MTPVTDTSKE GYYAISFSTG 610 620 630 640 650 660 AGYAMVSYQG PDIPWQKVIS TPSNPDKYEY VVEENKDLAE AAKKHELPIN IYGTINVDGV 670 680 690 700 710 720 DLNYVERRPP HFDKNKKYPV LFQQYSGPVS QTVKKTFAVD FQSFVAAGLG YICVTVDGRG 730 740 750 760 770 780 TGFIGRKNRV IIRGDLGHWE SHDQIAAAKH WAQKDYIDED RLAIWGWSYG GYMTLKTLEQ 790 800 810 820 830 840 DAGQTFKYGM AVAPVTDWRF YDSIYTERYM RTPQTNLEGY DSAAVTNATA LSQNVRFLLM 850 860 870 880 890 900 HGVADDNVHM QNSLTLLDAL DQRSVENYDV HVFPDSDHGI YFHNANRIVF DKLTNWLVNA 910 920 FNGEWLKIAN AQPNGMKKRA APTA

References

[1]

"De novo assembly of a 40 Mb eukaryotic genome from short sequence reads: Sordaria macrospora, a model organism for fungal morphogenesis." Nowrousian M., Stajich J.E., Chu M., Engh I., Espagne E., Halliday K., Kamerewerd J., Kempken F., Knab B., Kuo H.-C., Osiewacz H.D., Poeggeler S., Read N.D., Seiler S., Smith K.M., Zickler D., Kueck U., Freitag M. PLoS Genet. 6:E1000891-E1000891(2010) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC MYA-333 / DSM 997 / K(L3346) / K-hell.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CABT02000006 Genomic DNA. Translation: CCC08602.1.
RefSeq	XP_003351079.1. XM_003351031.1.
3D structure databases
ProteinModelPortal	D1Z9B4.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	10808655.
KEGG	smp:SMAC_05958.
Phylogenomic databases
KO	K01282.
Family and domain databases
InterPro	IPR002471. Pept_S9_AS. IPR001375. Peptidase_S9. IPR002469. Peptidase_S9B. [Graphical view]
Pfam	PF00930. DPPIV_N. 1 hit. PF00326. Peptidase_S9. 1 hit. [Graphical view]
PROSITE	PS00708. PRO_ENDOPEP_SER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DAPB_SORMK

Accession

Primary (citable) accession number: D1Z9B4
Secondary accession number(s): F7VTE6

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 27, 2011
Last sequence update:	February 9, 2010
Last modified:	October 3, 2012
This is version 21 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program