D1Z4I6 (LIPA_SORMK) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Lipoyl synthase, mitochondrial
EC=2.8.1.8

Alternative name(s):
Lipoate synthase
Short name=LS
Short name=Lip-syn
Lipoic acid synthase

Gene names

ORF Names:

SMAC_01256

Organism

Sordaria macrospora (strain ATCC MYA-333 / DSM 997 / K(L3346) / K-hell) [Complete proteome]

Taxonomic identifier

771870 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Pezizomycotina › leotiomyceta › sordariomyceta › Sordariomycetes › Sordariomycetidae › Sordariales › Sordariaceae › Sordaria › Sordaria macrospora

Protein attributes

Sequence length	445 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives By similarity. HAMAP-Rule MF_03123
Catalytic activity	Protein N(6)-(octanoyl)lysine + 2 sulfur + 2 S-adenosyl-L-methionine = protein N(6)-(lipoyl)lysine + 2 L-methionine + 2 5'-deoxyadenosine. HAMAP-Rule MF_03123
Cofactor	Binds 2 4Fe-4S clusters per subunit. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.
Pathway	Protein modification; protein lipoylation via endogenous pathway; protein N(6)-(lipoyl)lysine from octanoyl-[acyl-carrier-protein]: step 2/2. HAMAP-Rule MF_03123
Subcellular location	Mitochondrion Potential HAMAP-Rule MF_03123.
Sequence similarities	Belongs to the radical SAM superfamily. Lipoyl synthase family.

Ontologies

Keywords
Cellular component	Mitochondrion
Domain	Transit peptide
Ligand	4Fe-4S Iron Iron-sulfur Metal-binding S-adenosyl-L-methionine
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	protein lipoylation Inferred from electronic annotation. Source: HAMAP
Cellular_component	mitochondrion Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: HAMAP lipoate synthase activity Inferred from electronic annotation. Source: HAMAP metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 40	40	Mitochondrion Potential
	Chain	41 – 445	405	Lipoyl synthase, mitochondrial HAMAP-Rule MF_03123		PRO_0000398293
Sites
	Metal binding	157	1	Iron-sulfur 1 (4Fe-4S) By similarity
	Metal binding	162	1	Iron-sulfur 1 (4Fe-4S) By similarity
	Metal binding	168	1	Iron-sulfur 1 (4Fe-4S) By similarity
	Metal binding	188	1	Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity
	Metal binding	192	1	Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity
	Metal binding	195	1	Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity

Sequences

	Sequence		Length	Mass (Da)
	D1Z4I6 [UniParc]. Last modified February 9, 2010. Version 1. Checksum: CC87656ED8D9D672	FASTA	445	48,591
	10 20 30 40 50 60 MRPGSWRVIT HYGFTGPIQR LQAPLRRSLA RAAALSTRSY ATIPSAPSSQ PTSQESSPAA 70 80 90 100 110 120 SASASASAPA TKPRPTYFKD TTLASLDDFI ANQSSAAPLA PSEAYTLRTA QVGPAGKKRT 130 140 150 160 170 180 ITRLPEWLKT PIPSAGANPE FAKIKADLRG LNLHTVCEEA RCPNIGECWG GSNKAAATAT 190 200 210 220 230 240 IMLMGDTCTR GCRFCSVKTS RKPPPLDPHE PENTAEALAR WGLGYVVLTS VDRDDLADGG 250 260 270 280 290 300 ARHFAETIRR IKQKKPTLLV EALTGDFMGD LDMVKIVADS GLDVYAHNVE TVENLTPYVR 310 320 330 340 350 360 DRRATFRQSL KVLEHVKNVR GKEGIITKTS IMLGLGETEE ELWDALRELR KVDVDVVTFG 370 380 390 400 410 420 QYMRPTKRHL AVEKYITPDE FELWRQRALD MGFLYCASGP LVRSSYKAGE AFIENVLKKR 430 440 ASERVVSEAL GQAVAAEEAT SAKSA

References

[1]

"De novo assembly of a 40 Mb eukaryotic genome from short sequence reads: Sordaria macrospora, a model organism for fungal morphogenesis." Nowrousian M., Stajich J.E., Chu M., Engh I., Espagne E., Halliday K., Kamerewerd J., Kempken F., Knab B., Kuo H.-C., Osiewacz H.D., Poeggeler S., Read N.D., Seiler S., Smith K.M., Zickler D., Kueck U., Freitag M. PLoS Genet. 6:E1000891-E1000891(2010) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC MYA-333 / DSM 997 / K(L3346) / K-hell.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CABT02000004 Genomic DNA. Translation: CCC07689.1.
RefSeq	XP_003352422.1. XM_003352374.1.
3D structure databases
ProteinModelPortal	D1Z4I6.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	10810058.
KEGG	smp:SMAC_01256.
Phylogenomic databases
KO	K03644.
Enzyme and pathway databases
UniPathway	UPA00538; UER00593.
Family and domain databases
Gene3D	3.20.20.70. 1 hit.
HAMAP	MF_00206. Lipoyl_synth.
InterPro	IPR013785. Aldolase_TIM. IPR006638. Elp3/MiaB/NifB. IPR003698. Lipoyl_synth. IPR007197. rSAM. [Graphical view]
PANTHER	PTHR10949. PTHR10949. 1 hit.
Pfam	PF04055. Radical_SAM. 1 hit. [Graphical view]
SMART	SM00729. Elp3. 1 hit. [Graphical view]
TIGRFAMs	TIGR00510. lipA. 1 hit.
ProtoNet	Search...

Entry information

Entry name

LIPA_SORMK

Accession

Primary (citable) accession number: D1Z4I6
Secondary accession number(s): F7VQA7

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 5, 2010
Last sequence update:	February 9, 2010
Last modified:	April 3, 2013
This is version 24 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program