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D1Z4I6

- LIPA_SORMK

UniProt

D1Z4I6 - LIPA_SORMK

Protein

Lipoyl synthase, mitochondrial

Gene

SMAC_01256

Organism
Sordaria macrospora (strain ATCC MYA-333 / DSM 997 / K(L3346) / K-hell)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 30 (01 Oct 2014)
      Sequence version 1 (09 Feb 2010)
      Previous versions | rss
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    Functioni

    Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives.UniRule annotation

    Catalytic activityi

    Protein N(6)-(octanoyl)lysine + 2 sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = protein N(6)-(lipoyl)lysine + 2 (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.

    Cofactori

    Binds 2 4Fe-4S clusters per subunit. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi157 – 1571Iron-sulfur 1 (4Fe-4S)UniRule annotation
    Metal bindingi162 – 1621Iron-sulfur 1 (4Fe-4S)UniRule annotation
    Metal bindingi168 – 1681Iron-sulfur 1 (4Fe-4S)UniRule annotation
    Metal bindingi188 – 1881Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation
    Metal bindingi192 – 1921Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation
    Metal bindingi195 – 1951Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation

    GO - Molecular functioni

    1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-HAMAP
    2. lipoate synthase activity Source: UniProtKB-HAMAP
    3. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. protein lipoylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Transferase

    Keywords - Ligandi

    4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

    Enzyme and pathway databases

    UniPathwayiUPA00538; UER00593.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Lipoyl synthase, mitochondrialUniRule annotation (EC:2.8.1.8UniRule annotation)
    Alternative name(s):
    Lipoate synthaseUniRule annotation
    Short name:
    LSUniRule annotation
    Short name:
    Lip-synUniRule annotation
    Lipoic acid synthaseUniRule annotation
    Gene namesi
    ORF Names:SMAC_01256
    OrganismiSordaria macrospora (strain ATCC MYA-333 / DSM 997 / K(L3346) / K-hell)
    Taxonomic identifieri771870 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaSordariomycetesSordariomycetidaeSordarialesSordariaceaeSordaria
    ProteomesiUP000001881: Unassembled WGS sequence

    Subcellular locationi

    Mitochondrion UniRule annotation

    GO - Cellular componenti

    1. mitochondrion Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Mitochondrion

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transit peptidei1 – 4040MitochondrionUniRule annotationAdd
    BLAST
    Chaini41 – 445405Lipoyl synthase, mitochondrialPRO_0000398293Add
    BLAST

    Structurei

    3D structure databases

    ProteinModelPortaliD1Z4I6.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the radical SAM superfamily. Lipoyl synthase family.UniRule annotation

    Keywords - Domaini

    Transit peptide

    Phylogenomic databases

    KOiK03644.
    OrthoDBiEOG79KPR7.

    Family and domain databases

    Gene3Di3.20.20.70. 1 hit.
    HAMAPiMF_00206. Lipoyl_synth.
    InterProiIPR013785. Aldolase_TIM.
    IPR006638. Elp3/MiaB/NifB.
    IPR003698. Lipoyl_synth.
    IPR007197. rSAM.
    [Graphical view]
    PANTHERiPTHR10949. PTHR10949. 1 hit.
    PfamiPF04055. Radical_SAM. 1 hit.
    [Graphical view]
    SMARTiSM00729. Elp3. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR00510. lipA. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    D1Z4I6-1 [UniParc]FASTAAdd to Basket

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    MRPGSWRVIT HYGFTGPIQR LQAPLRRSLA RAAALSTRSY ATIPSAPSSQ    50
    PTSQESSPAA SASASASAPA TKPRPTYFKD TTLASLDDFI ANQSSAAPLA 100
    PSEAYTLRTA QVGPAGKKRT ITRLPEWLKT PIPSAGANPE FAKIKADLRG 150
    LNLHTVCEEA RCPNIGECWG GSNKAAATAT IMLMGDTCTR GCRFCSVKTS 200
    RKPPPLDPHE PENTAEALAR WGLGYVVLTS VDRDDLADGG ARHFAETIRR 250
    IKQKKPTLLV EALTGDFMGD LDMVKIVADS GLDVYAHNVE TVENLTPYVR 300
    DRRATFRQSL KVLEHVKNVR GKEGIITKTS IMLGLGETEE ELWDALRELR 350
    KVDVDVVTFG QYMRPTKRHL AVEKYITPDE FELWRQRALD MGFLYCASGP 400
    LVRSSYKAGE AFIENVLKKR ASERVVSEAL GQAVAAEEAT SAKSA 445
    Length:445
    Mass (Da):48,591
    Last modified:February 9, 2010 - v1
    Checksum:iCC87656ED8D9D672
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CABT02000004 Genomic DNA. Translation: CCC07689.1.
    RefSeqiXP_003352422.1. XM_003352374.1.

    Genome annotation databases

    GeneIDi10810058.
    KEGGismp:SMAC_01256.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CABT02000004 Genomic DNA. Translation: CCC07689.1 .
    RefSeqi XP_003352422.1. XM_003352374.1.

    3D structure databases

    ProteinModelPortali D1Z4I6.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 10810058.
    KEGGi smp:SMAC_01256.

    Phylogenomic databases

    KOi K03644.
    OrthoDBi EOG79KPR7.

    Enzyme and pathway databases

    UniPathwayi UPA00538 ; UER00593 .

    Family and domain databases

    Gene3Di 3.20.20.70. 1 hit.
    HAMAPi MF_00206. Lipoyl_synth.
    InterProi IPR013785. Aldolase_TIM.
    IPR006638. Elp3/MiaB/NifB.
    IPR003698. Lipoyl_synth.
    IPR007197. rSAM.
    [Graphical view ]
    PANTHERi PTHR10949. PTHR10949. 1 hit.
    Pfami PF04055. Radical_SAM. 1 hit.
    [Graphical view ]
    SMARTi SM00729. Elp3. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR00510. lipA. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "De novo assembly of a 40 Mb eukaryotic genome from short sequence reads: Sordaria macrospora, a model organism for fungal morphogenesis."
      Nowrousian M., Stajich J.E., Chu M., Engh I., Espagne E., Halliday K., Kamerewerd J., Kempken F., Knab B., Kuo H.-C., Osiewacz H.D., Poeggeler S., Read N.D., Seiler S., Smith K.M., Zickler D., Kueck U., Freitag M.
      PLoS Genet. 6:E1000891-E1000891(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC MYA-333 / DSM 997 / K(L3346) / K-hell.

    Entry informationi

    Entry nameiLIPA_SORMK
    AccessioniPrimary (citable) accession number: D1Z4I6
    Secondary accession number(s): F7VQA7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 5, 2010
    Last sequence update: February 9, 2010
    Last modified: October 1, 2014
    This is version 30 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3