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Protein

Bifunctional enzyme Fae/Hps

Gene

fae

Organism
Methanocella paludicola (strain DSM 17711 / JCM 13418 / NBRC 101707 / SANAE)
Status
Unreviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the condensation of formaldehyde with tetrahydromethanopterin (H4MPT) to 5,10-methylenetetrahydromethanopterin.UniRule annotation
Catalyzes the reversible formation of ribulose-5-phosphate and formaldehyde from 3-hexulose-6-phosphate.UniRule annotation

Catalytic activityi

5,6,7,8-tetrahydromethanopterin + formaldehyde = 5,10-methylenetetrahydromethanopterin + H2O.UniRule annotation
D-arabino-hex-3-ulose 6-phosphate = D-ribulose 5-phosphate + formaldehyde.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei17 – 171Proton donorUniRule annotation
Binding sitei19 – 191SubstrateUniRule annotation
Binding sitei48 – 481Substrate; via carbonyl oxygenUniRule annotation
Binding sitei66 – 661SubstrateUniRule annotation
Binding sitei68 – 681SubstrateUniRule annotation
Binding sitei83 – 831SubstrateUniRule annotation

GO - Molecular functioni

  1. carbon-nitrogen lyase activity Source: InterPro
  2. hexulose-6-phosphate synthase activity Source: UniProtKB-HAMAP
  3. hydro-lyase activity Source: UniProtKB-HAMAP
  4. orotidine-5'-phosphate decarboxylase activity Source: InterPro

GO - Biological processi

  1. 'de novo' pyrimidine nucleobase biosynthetic process Source: InterPro
  2. carbohydrate biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

LyaseUniRule annotation

Keywords - Biological processi

Carbohydrate metabolismUniRule annotation

Enzyme and pathway databases

BioCyciMPAL304371:GI7G-2965-MONOMER.
UniPathwayiUPA00293.

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional enzyme Fae/HpsUniRule annotation
Gene namesi
Name:faeImported
Synonyms:fae-hpsUniRule annotation, hpsImported
Ordered Locus Names:MCP_2904Imported
OrganismiMethanocella paludicola (strain DSM 17711 / JCM 13418 / NBRC 101707 / SANAE)Imported
Taxonomic identifieri304371 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanomicrobiaMethanocellalesMethanocellaceaeMethanocella
ProteomesiUP000001882 Componenti: Chromosome

Structurei

3D structure databases

ProteinModelPortaliD1Z2Q4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 161161Formaldehyde-activating enzymeUniRule annotationAdd
BLAST
Regioni162 – 3962353-hexulose-6-phosphate synthaseUniRule annotationAdd
BLAST

Sequence similaritiesi

In the C-terminal section; belongs to the HPS/KGPDC family. HPS subfamily.UniRule annotation
In the N-terminal section; belongs to the formaldehyde-activating enzyme family.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000286408.
KOiK13812.
OMAiYNYGATK.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
3.30.230.60. 1 hit.
HAMAPiMF_01268. Fae_Hps.
InterProiIPR013785. Aldolase_TIM.
IPR020868. Bifunctional_enzyme_fae/hps.
IPR014826. HCHO-activating_enzyme.
IPR001754. OMPdeCOase_dom.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR011060. RibuloseP-bd_barrel.
[Graphical view]
PfamiPF08714. Fae. 1 hit.
PF00215. OMPdecase. 1 hit.
[Graphical view]
SMARTiSM00934. OMPdecase. 1 hit.
[Graphical view]
SUPFAMiSSF51366. SSF51366. 1 hit.
SSF54211. SSF54211. 1 hit.
TIGRFAMsiTIGR03126. one_C_fae. 1 hit.

Sequencei

Sequence statusi: Complete.

D1Z2Q4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MYLVGEALIG EGNEVAHIDL LVGDKAGPVG MAFANGMTNM SAGHTPLLAV
60 70 80 90 100
VRPNLIPKPA TLIVPKVTVK NLEQAAQIFG PAQAAVAKAV ADAVEEGIIP
110 120 130 140 150
KDQVESIVII VSVFVHPAAK DYTRIYKYNY GATRLALVRA MESFPPVDKV
160 170 180 190 200
TFEKDRGTHA IMGYKIMRLW DPPYLQIAID APDLGVVERV LMQAPKNDHI
210 220 230 240 250
IIEAGTPLIK RYGLEVISKI RAIKKDAFIV ADLKTLDTGN LEARMAADAT
260 270 280 290 300
ADAVVCSGLA PLETIEKFCE EARKVGIYSI IDMLNVDNPA KVVEALKHKP
310 320 330 340 350
DIVELHRGID TEGQKAEHAW GNIAGIKKAA GGKKLLVAVA GGVKVENVEV
360 370 380 390
AMKGGADILV VGRAITNAKD IEGATRAFLR AMHKDEIDQY RIMTDF
Length:396
Mass (Da):42,680
Last modified:February 9, 2010 - v1
Checksum:i956B14C37D4C77E4
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP011532 Genomic DNA. Translation: BAI62976.1.
RefSeqiYP_003357959.1. NC_013665.1.

Genome annotation databases

EnsemblBacteriaiBAI62976; BAI62976; MCP_2904.
GeneIDi8682577.
KEGGimpd:MCP_2904.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP011532 Genomic DNA. Translation: BAI62976.1.
RefSeqiYP_003357959.1. NC_013665.1.

3D structure databases

ProteinModelPortaliD1Z2Q4.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAI62976; BAI62976; MCP_2904.
GeneIDi8682577.
KEGGimpd:MCP_2904.

Phylogenomic databases

HOGENOMiHOG000286408.
KOiK13812.
OMAiYNYGATK.

Enzyme and pathway databases

UniPathwayiUPA00293.
BioCyciMPAL304371:GI7G-2965-MONOMER.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
3.30.230.60. 1 hit.
HAMAPiMF_01268. Fae_Hps.
InterProiIPR013785. Aldolase_TIM.
IPR020868. Bifunctional_enzyme_fae/hps.
IPR014826. HCHO-activating_enzyme.
IPR001754. OMPdeCOase_dom.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR011060. RibuloseP-bd_barrel.
[Graphical view]
PfamiPF08714. Fae. 1 hit.
PF00215. OMPdecase. 1 hit.
[Graphical view]
SMARTiSM00934. OMPdecase. 1 hit.
[Graphical view]
SUPFAMiSSF51366. SSF51366. 1 hit.
SSF54211. SSF54211. 1 hit.
TIGRFAMsiTIGR03126. one_C_fae. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Genome sequence of a mesophilic hydrogenotrophic methanogen Methanocella paludicola, the first cultivated representative of the order Methanocellales."
    Sakai S., Takaki Y., Shimamura S., Sekine M., Tajima T., Kosugi H., Ichikawa N., Tasumi E., Hiraki A.T., Shimizu A., Kato Y., Nishiko R., Mori K., Fujita N., Imachi H., Takai K.
    PLoS ONE 6:E22898-E22898(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: DSM 17711 / JCM 13418 / NBRC 101707 / SANAEImported.

Entry informationi

Entry nameiD1Z2Q4_METPS
AccessioniPrimary (citable) accession number: D1Z2Q4
Entry historyi
Integrated into UniProtKB/TrEMBL: February 9, 2010
Last sequence update: February 9, 2010
Last modified: February 4, 2015
This is version 30 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzymeUniRule annotation, Reference proteomeImported

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.