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Protein

Geraniol 8-hydroxylase

Gene

CYP76B10

Organism
Swertia mussotii (Felwort)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Hydroxylase involved in the biosynthesis of hydroxygeraniol, a precursor of the iridoid monoterpenoid swertiamarin.1 Publication

Miscellaneous

The recommended numbering of geraniol gives (6E)-8-hydroxygeraniol as the product rather than 10-hydroxygeraniol as used in most publications.

Catalytic activityi

Geraniol + NADPH + O2 = (6E)-8-hydroxygeraniol + NADP+ + H2O.1 Publication

Cofactori

hemeBy similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi438Iron (heme axial ligand)By similarity1

GO - Molecular functioni

GO - Biological processi

  • monoterpenoid biosynthetic process Source: UniProtKB

Keywordsi

Molecular functionMonooxygenase, Oxidoreductase
LigandHeme, Iron, Metal-binding, NADP

Enzyme and pathway databases

BRENDAi1.14.13.152. 12614.

Names & Taxonomyi

Protein namesi
Recommended name:
Geraniol 8-hydroxylase (EC:1.14.13.152)
Alternative name(s):
Cytochrome P450 76B10
Geraniol 10-hydroxylase
Short name:
SmG10H
Gene namesi
Name:CYP76B10
Synonyms:G10H
OrganismiSwertia mussotii (Felwort)
Taxonomic identifieri137888 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeasteridslamiidsGentianalesGentianaceaeGentianeaeSwertia

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei5 – 25HelicalSequence analysisAdd BLAST21

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004189201 – 495Geraniol 8-hydroxylaseAdd BLAST495

Expressioni

Tissue specificityi

Expressed in leaves, stems and roots.1 Publication

Inductioni

Up-regulated by methyl jasmonate treatment.1 Publication

Structurei

3D structure databases

ProteinModelPortaliD1MI46.
SMRiD1MI46.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the cytochrome P450 family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

KOiK15099.

Family and domain databases

Gene3Di1.10.630.10. 1 hit.
InterProiView protein in InterPro
IPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
IPR036396. Cyt_P450_sf.
PfamiView protein in Pfam
PF00067. p450. 1 hit.
PRINTSiPR00463. EP450I.
PR00385. P450.
SUPFAMiSSF48264. SSF48264. 1 hit.
PROSITEiView protein in PROSITE
PS00086. CYTOCHROME_P450. 1 hit.

Sequencei

Sequence statusi: Complete.

D1MI46-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDFDFLTIAI GFLFTITLYQ ALNFFSRKSK NLPPGPSPLP LIGNLHLLGD
60 70 80 90 100
QPHKSLAKLA KKHGPIMGLQ LGQVTTIVVT SSGMAKEVLQ KQDLAFSSRS
110 120 130 140 150
IPNAIHAHDQ YKYSVIWLPV ASRWRGLRKA LNSNMFSGNR LDANQHLRSR
160 170 180 190 200
KVQELIAYCR KSSQTGDAID VGRAAFRTSL NLLSNTMFSK DLTDPYSDSA
210 220 230 240 250
KEFKDLVWNV MVEAGKPNLV DYFPLLDKVD PQGIRKRMTI HFGKILELFG
260 270 280 290 300
GLIDERLQQK KAKGVNDDVL DVLLTTSEES PEEIDRTHIQ RMCLDLFVAG
310 320 330 340 350
TDTTSSTLEW AMSEMLKNPE KMKAAQAELA QVIGKGKAVE EADLARLPYL
360 370 380 390 400
RCAIKETLRI HPPVPLLIPR RTEQEVEVCG YTVPKNSQVL VNVWAISRDD
410 420 430 440 450
AIWKDPLSFK PERFLESELE MRGKDFELIP FGAGRRICPG LPLAVRMVPV
460 470 480 490
MLGSLLNSFD WKLEGGIAPK DLDMEEKFGI TLQKAHPLRA VATPL
Length:495
Mass (Da):55,456
Last modified:January 19, 2010 - v1
Checksum:iC84723BF767B49F6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
GU168041 mRNA. Translation: ACZ48680.1.

Genome annotation databases

KEGGiag:ACZ48680.

Similar proteinsi

Entry informationi

Entry nameiC76BA_SWEMU
AccessioniPrimary (citable) accession number: D1MI46
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 5, 2012
Last sequence update: January 19, 2010
Last modified: October 25, 2017
This is version 31 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families