Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

D1J7E5 (D1J7E5_MYCHP) Unreviewed, UniProtKB/TrEMBL

Last modified April 16, 2014. Version 33. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry infoCustomize order

Names and origin

Protein namesRecommended name:
Ribosomal RNA small subunit methyltransferase A HAMAP-Rule MF_00607

EC=2.1.1.182 HAMAP-Rule MF_00607
Alternative name(s):
16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))-dimethyltransferase HAMAP-Rule MF_00607
16S rRNA dimethyladenosine transferase HAMAP-Rule MF_00607
16S rRNA dimethylase HAMAP-Rule MF_00607
S-adenosylmethionine-6-N', N'-adenosyl(rRNA) dimethyltransferase HAMAP-Rule MF_00607
Gene names
Name:rsmA HAMAP-Rule MF_00607
Synonyms:ksgA HAMAP-Rule MF_00607 EMBL CAX37142.1
Ordered Locus Names:MHO_0080 EMBL CAX37142.1
OrganismMycoplasma hominis (strain ATCC 23114 / NBRC 14850 / NCTC 10111 / PG21) [Complete proteome] [HAMAP] EMBL CAX37142.1
Taxonomic identifier347256 [NCBI]
Taxonomic lineageBacteriaTenericutesMollicutesMycoplasmataceaeMycoplasma

Protein attributes

Sequence length257 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Specifically dimethylates two adjacent adenosines (A1518 and A1519) in the loop of a conserved hairpin near the 3'-end of 16S rRNA in the 30S particle. May play a critical role in biogenesis of 30S subunits By similarity. HAMAP-Rule MF_00607 SAAS SAAS020596

Catalytic activity

4 S-adenosyl-L-methionine + adenine(1518)/adenine(1519) in 16S rRNA = 4 S-adenosyl-L-homocysteine + N(6)-dimethyladenine(1518)/N(6)-dimethyladenine(1519) in 16S rRNA. HAMAP-Rule MF_00607 SAAS SAAS020596

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00607 SAAS SAAS023165.

Sequence similarities

Belongs to the class I-like SAM-binding methyltransferase superfamily. rRNA adenine N(6)-methyltransferase family. RsmA subfamily. HAMAP-Rule MF_00607

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Sites

Binding site111S-adenosyl-L-methionine; via carbonyl oxygen By similarity HAMAP-Rule MF_00607
Binding site131S-adenosyl-L-methionine; via amide nitrogen By similarity HAMAP-Rule MF_00607
Binding site371S-adenosyl-L-methionine; via carbonyl oxygen By similarity HAMAP-Rule MF_00607
Binding site581S-adenosyl-L-methionine By similarity HAMAP-Rule MF_00607
Binding site821S-adenosyl-L-methionine By similarity HAMAP-Rule MF_00607
Binding site991S-adenosyl-L-methionine By similarity HAMAP-Rule MF_00607

Sequences

Sequence LengthMass (Da)Tools
D1J7E5 [UniParc].

Last modified January 19, 2010. Version 1.
Checksum: BD6EDED4667893CA

FASTA25729,513
        10         20         30         40         50         60 
MIKALKSLGQ NFLSNKNIQK EIVKAANVVG KNIIEIGPGM GAITDQMADV VNRLVCIEFD 

        70         80         90        100        110        120 
KRLYEFLLQK NYSSNVEILN QDFLQTDLLK YTDFEVIGNI PYNITSDIIF KLLDNAKNIN 

       130        140        150        160        170        180 
KITLMVQKEV ADRICCGAGN SQYSKLAASI QLLYEPKYLF TVKAKEFNPA PKVDSAVIQL 

       190        200        210        220        230        240 
NLIKNNDFIW NNQVEILKFI KQMFQYKRKT LLNNFPSNLK QKISDFLKSN ELDLNIRAEK 

       250 
ITKEISINLF KFINNKN 

« Hide

References

[1]"Life on arginine for Mycoplasma hominis: clues from its minimal genome and comparison with other human urogenital mycoplasmas."
Pereyre S., Sirand-Pugnet P., Beven L., Charron A., Renaudin H., Barre A., Avenaud P., Jacob D., Couloux A., Barbe V., de Daruvar A., Blanchard A., Bebear C.
PLoS Genet. 5:E1000677-E1000677(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 23114 / NBRC 14850 / NCTC 10111 / PG21.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
FP236530 Genomic DNA. Translation: CAX37142.1.
RefSeqYP_003302545.1. NC_013511.1.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAX37142; CAX37142; MHO_0080.
GeneID8595423.
KEGGmho:MHO_0080.
PATRIC32273075. VBIMycHom120329_0008.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMHOG000227962.
KOK02528.
OMARLSAMLY.
OrthoDBEOG66F08Z.

Enzyme and pathway databases

BioCycMHOM347256:GBZD-8-MONOMER.

Family and domain databases

Gene3D1.10.8.100. 1 hit.
HAMAPMF_00607. 16SrRNA_methyltr_A.
InterProIPR023165. rRNA_Ade_diMease-like.
IPR020596. rRNA_Ade_Mease_Trfase_CS.
IPR001737. rRNA_Ade_methylase_transferase.
IPR020598. rRNA_Ade_methylase_Trfase_N.
IPR011530. rRNA_adenine_dimethylase.
[Graphical view]
PANTHERPTHR11727. PTHR11727. 1 hit.
PfamPF00398. RrnaAD. 1 hit.
[Graphical view]
SMARTSM00650. rADc. 1 hit.
[Graphical view]
TIGRFAMsTIGR00755. ksgA. 1 hit.
PROSITEPS01131. RRNA_A_DIMETH. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameD1J7E5_MYCHP
AccessionPrimary (citable) accession number: D1J7E5
Entry history
Integrated into UniProtKB/TrEMBL: January 19, 2010
Last sequence update: January 19, 2010
Last modified: April 16, 2014
This is version 33 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)