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Protein

Ribosomal RNA small subunit methyltransferase A

Gene

rsmA

Organism
Mycoplasma hominis (strain ATCC 23114 / NBRC 14850 / NCTC 10111 / PG21)
Status
Unreviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Specifically dimethylates two adjacent adenosines (A1518 and A1519) in the loop of a conserved hairpin near the 3'-end of 16S rRNA in the 30S particle. May play a critical role in biogenesis of 30S subunits.UniRule annotationSAAS annotation

Catalytic activityi

4 S-adenosyl-L-methionine + adenine(1518)/adenine(1519) in 16S rRNA = 4 S-adenosyl-L-homocysteine + N(6)-dimethyladenine(1518)/N(6)-dimethyladenine(1519) in 16S rRNA.UniRule annotationSAAS annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei11 – 111S-adenosyl-L-methionine; via carbonyl oxygenUniRule annotation
Binding sitei13 – 131S-adenosyl-L-methionine; via amide nitrogenUniRule annotation
Binding sitei37 – 371S-adenosyl-L-methionine; via carbonyl oxygenUniRule annotation
Binding sitei58 – 581S-adenosyl-L-methionineUniRule annotation
Binding sitei82 – 821S-adenosyl-L-methionineUniRule annotation
Binding sitei99 – 991S-adenosyl-L-methionineUniRule annotation

GO - Molecular functioni

  1. 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))-dimethyltransferase activity Source: UniProtKB-EC
  2. RNA binding Source: UniProtKB-KW
  3. rRNA (adenine-N6,N6-)-dimethyltransferase activity Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

MethyltransferaseUniRule annotationSAAS annotation, Transferase

Keywords - Biological processi

rRNA processingUniRule annotationSAAS annotation

Keywords - Ligandi

RNA-bindingUniRule annotationSAAS annotation, S-adenosyl-L-methionineUniRule annotationSAAS annotation

Enzyme and pathway databases

BioCyciMHOM347256:GBZD-8-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Ribosomal RNA small subunit methyltransferase AUniRule annotationSAAS annotation (EC:2.1.1.182UniRule annotationSAAS annotation)
Alternative name(s):
16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))-dimethyltransferaseUniRule annotation
16S rRNA dimethyladenosine transferaseUniRule annotation
16S rRNA dimethylaseUniRule annotation
S-adenosylmethionine-6-N', N'-adenosyl(rRNA) dimethyltransferaseUniRule annotation
Gene namesi
Name:rsmAUniRule annotation
Synonyms:ksgAUniRule annotationImported
Ordered Locus Names:MHO_0080Imported
OrganismiMycoplasma hominis (strain ATCC 23114 / NBRC 14850 / NCTC 10111 / PG21)Imported
Taxonomic identifieri347256 [NCBI]
Taxonomic lineageiBacteriaTenericutesMollicutesMycoplasmataceaeMycoplasma
ProteomesiUP000002631: Chromosome

Subcellular locationi

Cytoplasm UniRule annotationSAAS annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

CytoplasmUniRule annotationSAAS annotation

Family & Domainsi

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. rRNA adenine N(6)-methyltransferase family. RsmA subfamily.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000227962.
KOiK02528.
OMAiTAYEVDT.
OrthoDBiEOG66F08Z.

Family and domain databases

Gene3Di1.10.8.100. 1 hit.
3.40.50.150. 1 hit.
HAMAPiMF_00607. 16SrRNA_methyltr_A.
InterProiIPR001737. KsgA/Erm.
IPR023165. rRNA_Ade_diMease-like.
IPR020596. rRNA_Ade_Mease_Trfase_CS.
IPR020598. rRNA_Ade_methylase_Trfase_N.
IPR011530. rRNA_adenine_dimethylase.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PANTHERiPTHR11727. PTHR11727. 1 hit.
PfamiPF00398. RrnaAD. 1 hit.
[Graphical view]
SMARTiSM00650. rADc. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00755. ksgA. 1 hit.
PROSITEiPS01131. RRNA_A_DIMETH. 1 hit.
PS51689. SAM_RNA_A_N6_MT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

D1J7E5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIKALKSLGQ NFLSNKNIQK EIVKAANVVG KNIIEIGPGM GAITDQMADV
60 70 80 90 100
VNRLVCIEFD KRLYEFLLQK NYSSNVEILN QDFLQTDLLK YTDFEVIGNI
110 120 130 140 150
PYNITSDIIF KLLDNAKNIN KITLMVQKEV ADRICCGAGN SQYSKLAASI
160 170 180 190 200
QLLYEPKYLF TVKAKEFNPA PKVDSAVIQL NLIKNNDFIW NNQVEILKFI
210 220 230 240 250
KQMFQYKRKT LLNNFPSNLK QKISDFLKSN ELDLNIRAEK ITKEISINLF

KFINNKN
Length:257
Mass (Da):29,513
Last modified:January 19, 2010 - v1
Checksum:iBD6EDED4667893CA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FP236530 Genomic DNA. Translation: CAX37142.1.
RefSeqiYP_003302545.1. NC_013511.1.

Genome annotation databases

EnsemblBacteriaiCAX37142; CAX37142; MHO_0080.
GeneIDi8595423.
KEGGimho:MHO_0080.
PATRICi32273075. VBIMycHom120329_0008.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FP236530 Genomic DNA. Translation: CAX37142.1.
RefSeqiYP_003302545.1. NC_013511.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAX37142; CAX37142; MHO_0080.
GeneIDi8595423.
KEGGimho:MHO_0080.
PATRICi32273075. VBIMycHom120329_0008.

Phylogenomic databases

HOGENOMiHOG000227962.
KOiK02528.
OMAiTAYEVDT.
OrthoDBiEOG66F08Z.

Enzyme and pathway databases

BioCyciMHOM347256:GBZD-8-MONOMER.

Family and domain databases

Gene3Di1.10.8.100. 1 hit.
3.40.50.150. 1 hit.
HAMAPiMF_00607. 16SrRNA_methyltr_A.
InterProiIPR001737. KsgA/Erm.
IPR023165. rRNA_Ade_diMease-like.
IPR020596. rRNA_Ade_Mease_Trfase_CS.
IPR020598. rRNA_Ade_methylase_Trfase_N.
IPR011530. rRNA_adenine_dimethylase.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PANTHERiPTHR11727. PTHR11727. 1 hit.
PfamiPF00398. RrnaAD. 1 hit.
[Graphical view]
SMARTiSM00650. rADc. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00755. ksgA. 1 hit.
PROSITEiPS01131. RRNA_A_DIMETH. 1 hit.
PS51689. SAM_RNA_A_N6_MT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Life on arginine for Mycoplasma hominis: clues from its minimal genome and comparison with other human urogenital mycoplasmas."
    Pereyre S., Sirand-Pugnet P., Beven L., Charron A., Renaudin H., Barre A., Avenaud P., Jacob D., Couloux A., Barbe V., de Daruvar A., Blanchard A., Bebear C.
    PLoS Genet. 5:E1000677-E1000677(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 23114 / NBRC 14850 / NCTC 10111 / PG21Imported.

Entry informationi

Entry nameiD1J7E5_MYCHP
AccessioniPrimary (citable) accession number: D1J7E5
Entry historyi
Integrated into UniProtKB/TrEMBL: January 19, 2010
Last sequence update: January 19, 2010
Last modified: March 4, 2015
This is version 42 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteomeImported

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.