D1C2Z5 (D1C2Z5_SPHTD) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
Methionyl-tRNA formyltransferase HAMAP-Rule MF_00182
EC=2.1.2.9 HAMAP-Rule MF_00182

Gene names

Name:	fmt HAMAP-Rule MF_00182
Ordered Locus Names:	Sthe_1176 EMBL ACZ38612.1

Organism

Sphaerobacter thermophilus (strain DSM 20745 / S 6022) [Complete proteome] [HAMAP] EMBL ACZ38612.1

Taxonomic identifier

479434 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Chloroflexi › Thermomicrobia › Sphaerobacteridae › Sphaerobacterales › Sphaerobacterineae › Sphaerobacteraceae › Sphaerobacter › Sphaerobacter thermophilus

Protein attributes

Sequence length	314 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Modifies the free amino group of the aminoacyl moiety of methionyl-tRNA(fMet). The formyl group appears to play a dual role in the initiator identity of N-formylmethionyl-tRNA by: (I) promoting its recognition by IF2 and (II) impairing its binding to EFTu-GTP By similarity. HAMAP-Rule MF_00182 SAAS SAAS005794
Catalytic activity	10-formyltetrahydrofolate + L-methionyl-tRNA(fMet) = tetrahydrofolate + N-formylmethionyl-tRNA(fMet). HAMAP-Rule MF_00182 SAAS SAAS005794
Sequence similarities	Belongs to the fmt family. HAMAP-Rule MF_00182

Ontologies

Keywords
Biological process	Protein biosynthesis HAMAP-Rule MF_00182 SAAS SAAS005794
Domain	Signal EMBL ACZ38612.1
Molecular function	Methyltransferase HAMAP-Rule MF_00182 SAAS SAAS005794 Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	translational initiation Inferred from electronic annotation. Source: GOC
Molecular_function	methionyl-tRNA formyltransferase activity Inferred from electronic annotation. Source: HAMAP methyltransferase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 23	23	Potential EMBL ACZ38612.1
	Chain	24 – 314	291	Potential		PRO_5000537677
Regions
	Region	112 – 115	4	Tetrahydrofolate (THF) binding By similarity HAMAP-Rule MF_00182

Sequences

	Sequence		Length	Mass (Da)
	D1C2Z5 [UniParc]. Last modified January 19, 2010. Version 1. Checksum: 3BBACF9AA3D417ED	FASTA	314	33,662
	10 20 30 40 50 60 MAISVVFLGS PAFAVPSLRA LALDARFTIP LVVTQPDRPA GRGRRPRPPA VKDAAIELGL 70 80 90 100 110 120 PVFQPETLRD PAAVERLAAA VPDVLVVVAY GEILRQSVLD LAPLGCLNVH PSLLPRYRGS 130 140 150 160 170 180 SPVQAAILNG DTETGISIIK LVRRMDAGPI VAQRRVPLDG TETAGTLSER LANLAAEMLP 190 200 210 220 230 240 DVVAAWVAGE LEAEPQDDAA ATYTRELTTA DARIDWGKDA AEIERLVRAM QPWPKAWSIL 250 260 270 280 290 300 EGRRLAVLAC DISHKPSTEP PGTINVSARP PRVATGTTDL VLLRVQPEGK REMAAEDWAR 310 GARLPHGARF APVE

References

[1]

"The complete chromosome 1 of Sphaerobacter thermophilus DSM 20745." Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Kyrpides N., Mavromatis K., Ivanova N., Mikhailova N., LaButti K.M., Clum A., Sun H.I., Brettin T., Detter J.C. , Han C., Larimer F., Land M., Hauser L., Markowitz V., Cheng J.F., Hugenholtz P., Woyke T., Wu D., Steenblock K., Schneider S., Pukall R., Goeker M., Klenk H.P., Eisen J.A. Submitted (NOV-2009) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 20745 / S 6022.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001823 Genomic DNA. Translation: ACZ38612.1.
RefSeq	YP_003319434.1. NC_013523.1.
3D structure databases
ProteinModelPortal	D1C2Z5.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ACZ38612; ACZ38612; Sthe_1176.
GeneID	8638573.
KEGG	sti:Sthe_1176.
PATRIC	32425101. VBISphThe120955_1190.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HOG000261177.
KO	K00604.
OMA	GITLMQM.
Family and domain databases
Gene3D	3.10.25.10. 1 hit. 3.40.50.170. 1 hit.
HAMAP	MF_00182. Formyl_trans.
InterPro	IPR005794. Fmt. IPR005793. Formyl_trans_C. IPR002376. Formyl_transf_N. IPR011034. Formyl_transferase_C-like. IPR015518. Met_tRNA_Form_TA-like. [Graphical view]
PANTHER	PTHR11138. PTHR11138. 1 hit.
Pfam	PF02911. Formyl_trans_C. 1 hit. PF00551. Formyl_trans_N. 1 hit. [Graphical view]
SUPFAM	SSF50486. FMT_C_like. 1 hit. SSF53328. formyl_transf. 1 hit.
TIGRFAMs	TIGR00460. fmt. 1 hit.
ProtoNet	Search...

Entry information

Entry name

D1C2Z5_SPHTD

Accession

Primary (citable) accession number: D1C2Z5

Entry history

Integrated into UniProtKB/TrEMBL:	January 19, 2010
Last sequence update:	January 19, 2010
Last modified:	May 1, 2013
This is version 26 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)