D1BS17 (MSHC_XYLCX) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
L-cysteine:1D-myo-inositol 2-amino-2-deoxy-alpha-D-glucopyranoside ligase
Short name=L-Cys:GlcN-Ins ligase
EC=6.3.1.13

Alternative name(s):
Mycothiol ligase
Short name=MSH ligase

Gene names

Name:	mshC
Ordered Locus Names:	Xcel_1479

Organism

Xylanimonas cellulosilytica (strain DSM 15894 / CECT 5975 / LMG 20990 / XIL07) [Complete proteome] [HAMAP]

Taxonomic identifier

446471 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Actinobacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Micrococcineae › Promicromonosporaceae › Xylanimonas › Xylanimonas cellulosilytica

Protein attributes

Sequence length	404 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the ATP-dependent condensation of GlcN-Ins and L-cysteine to form L-Cys-GlcN-Ins By similarity. HAMAP-Rule MF_01697
Catalytic activity	1-O-(2-amino-2-deoxy-alpha-D-glucopyranosyl)-1D-myo-inositol + L-cysteine + ATP = 1-O-(2-(L-cysteinamido)-2-deoxy-alpha-D-glucopyranosyl)-1D-myo-inositol + AMP + diphosphate. HAMAP-Rule MF_01697
Cofactor	Binds 1 zinc ion per subunit By similarity.
Subunit structure	Monomer By similarity.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family. MshC subfamily.

Ontologies

Keywords
Ligand	ATP-binding Metal-binding Nucleotide-binding Zinc
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	mycothiol biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW cysteine-glucosaminylinositol ligase activity Inferred from electronic annotation. Source: HAMAP zinc ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 404	404	L-cysteine:1D-myo-inositol 2-amino-2-deoxy-alpha-D-glucopyranoside ligase HAMAP-Rule MF_01697		PRO_0000400494
Regions
	Region	45 – 48	4	Cysteinyl adenylate binding By similarity
	Region	83 – 85	3	Cysteinyl adenylate binding By similarity
	Region	248 – 250	3	Cysteinyl adenylate binding By similarity
	Motif	47 – 57	11	"HIGH" region By similarity
	Motif	185 – 190	6	"ERGGDP" region By similarity
	Motif	286 – 290	5	"KMSKS" region By similarity
Sites
	Metal binding	45	1	Zinc By similarity
	Metal binding	230	1	Zinc By similarity
	Metal binding	255	1	Zinc By similarity
	Binding site	60	1	Cysteinyl adenylate By similarity
	Binding site	226	1	Cysteinyl adenylate By similarity
	Binding site	280	1	Cysteinyl adenylate; via amide nitrogen and carbonyl oxygen By similarity

Sequences

	Sequence		Length	Mass (Da)
	D1BS17 [UniParc]. Last modified January 19, 2010. Version 1. Checksum: CEB3050CBEEFA536	FASTA	404	43,002
	10 20 30 40 50 60 MRTWPTPDVP PLPRTGAPAP VLVHDSTTQS LVEAAPGATA TLYACGITPY DATHLGHANT 70 80 90 100 110 120 YLAFDLLQRA WLDAGKTVVY TSNVTDVDDP LLERATATGV DWRELAREQT ELYRTDMTAL 130 140 150 160 170 180 RMLPPATWTG AVESIPAVVE AVTALLDAGA AYRVDADVYA DLSADPGFGR VAGLDDATMR 190 200 210 220 230 240 ALFAERGGDP DRPGKKHPLD PALWRGEQPG EPSWDGGKLG PGRPGWHIEC AVIARDGLGL 250 260 270 280 290 300 PFDVQGGGAD LLFPHHEMST SHARLLAGGA ARVHVHAGLL AYDGHKMSKS RGNLVFVSRL 310 320 330 340 350 360 LAAGTDPMTV RLALLAHHYR EEWEWTDVEL RTAQRRLDTW TSAILSTHDD GEPADTVLDA 370 380 390 400 VRAALAADLD APAALAAVDR WAANPGGDAG VVVATVDALL GIEL

References

[1]

"The complete chromosome of Xylanimonas cellulosilytica DSM 15894." US DOE Joint Genome Institute (JGI-PGF) Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Kyrpides N., Mavromatis K., Ivanova N., Mikhailova N., Foster B., Clum A., Brettin T., Detter J.C., Han C. , Larimer F., Land M., Hauser L., Markowitz V., Cheng J.F., Hugenholtz P., Woyke T., Wu D., Gehrich-Schroeter G., Schneider S., Pukall S.R., Klenk H.P., Eisen J.A. Submitted (NOV-2009) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 15894 / CECT 5975 / LMG 20990 / XIL07.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001821 Genomic DNA. Translation: ACZ30509.1.
RefSeq	YP_003326067.1. NC_013530.1.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ACZ30509; ACZ30509; Xcel_1479.
GeneID	8649001.
KEGG	xce:Xcel_1479.
PATRIC	32551959. VBIXylCel24446_1518.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HOG000245251.
KO	K15526.
OMA	DVHYVQN.
Family and domain databases
Gene3D	3.40.50.620. 1 hit.
HAMAP	MF_01697. MshC.
InterPro	IPR024909. Cys-tRNA/MSH_ligase. IPR017812. Mycothiol_ligase_MshC. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
PANTHER	PTHR10890. PTHR10890. 1 hit.
Pfam	PF01406. tRNA-synt_1e. 1 hit. [Graphical view]
PRINTS	PR00983. TRNASYNTHCYS.
TIGRFAMs	TIGR03447. mycothiol_MshC. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MSHC_XYLCX

Accession

Primary (citable) accession number: D1BS17

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 2, 2010
Last sequence update:	January 19, 2010
Last modified:	May 1, 2013
This is version 24 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program