D1BIM2 (D1BIM2_SANKS) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-alanine ligase RuleBase RU004136
EC=6.3.2.10 RuleBase RU004136

Gene names

Ordered Locus Names:

Sked_22840 EMBL ACZ22199.1

Organism

Sanguibacter keddieii (strain ATCC 51767 / DSM 10542 / NCFB 3025 / ST-74) [Complete proteome] [HAMAP] EMBL ACZ22199.1

Taxonomic identifier

446469 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Actinobacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Micrococcineae › Sanguibacteraceae › Sanguibacter › Sanguibacter keddieii

Protein attributes

Sequence length	464 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Involved in cell wall formation. Catalyzes the final step in the synthesis of UDP-N-acetylmuramoyl-pentapeptide, the precursor of murein By similarity.
Catalytic activity	ATP + UDP-N-acetylmuramoyl-L-alanyl-gamma-D-glutamyl-L-lysine + D-alanyl-D-alanine = ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-gamma-D-glutamyl-L-lysyl-D-alanyl-D-alanine.
Pathway	Cell wall biogenesis; peptidoglycan biosynthesis.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the MurCDEF family. RuleBase RU003630

Ontologies

Keywords
Biological process	Cell cycle Cell division RuleBase RU004136 Cell shape Cell wall biogenesis/degradation RuleBase RU004136 Peptidoglycan synthesis RuleBase RU004136
Ligand	ATP-binding RuleBase RU003630 Nucleotide-binding
Molecular function	Ligase RuleBase RU003630 EMBL ACZ22199.1
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase activity Inferred from electronic annotation. Source: EC UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-diaminopimelate-D-alanyl-D-alanine ligase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequences

	Sequence		Length	Mass (Da)
	D1BIM2 [UniParc]. Last modified January 19, 2010. Version 1. Checksum: 1F2A4BC78AFF2AB1	FASTA	464	47,650
	10 20 30 40 50 60 MIDLTAAEVA DLTGGTLAAP HTTRVHGPTV VDSRKVSAGA LFVALVGESS DGHDHAAGAV 70 80 90 100 110 120 ADGAALVLAS RELDGLPCVV VDDVTRALGL VAAEHLRRLR ASGPLQVAAV TGSVGKTTTK 130 140 150 160 170 180 DLLGHVLSES GPTVMPRASF NNEIGLPLTV LEAGPETRYL VLEMGASAPG DLTYLTGIAP 190 200 210 220 230 240 PDVSIVLAVG AAHLGGFGGL DGVARAKSEI VTGLRPEGVA VLNADDHRVV AMRDLAPSRV 250 260 270 280 290 300 VTFGTVRDAG VRAHRVSVDR LGRVSCQVVV GERTADLSLR LVGEHHLTNA LAALTAAVEL 310 320 330 340 350 360 GVDLDTAAAL VSSVDAASPH RMAVTERPDG VTVIDDSYNA NPDSMRAALK ALAVVAGRDR 370 380 390 400 410 420 RSVAVLGEML ELGPDSRDQH DEIGRLVVRL NIKLLVVVGA GASGLADGAT QEGSWGEEVV 430 440 450 460 EVEDVPAAER FLAEELRSGD VVLVKSSNGS GLWRLGDALT AVTS

References

[1]

"Complete genome sequence of Sanguibacter keddieii type strain (ST-74)." Ivanova N., Sikorski J., Sims D., Brettin T., Detter J.C., Han C., Lapidus A., Copeland A., Glavina Del Rio T., Nolan M., Chen F., Lucas S., Tice H., Cheng J.F., Bruce D., Goodwin L., Pitluck S., Pati A. , Mavrommatis K., Chen A., Palaniappan K., D'haeseleer P., Chain P., Bristow J., Eisen J.A., Markowitz V., Hugenholtz P., Goker M., Pukall R., Klenk H.P., Kyrpides N.C. Stand. Genomic Sci. 1:110-118(2009) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 51767 / DSM 10542 / NCFB 3025 / ST-74.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001819 Genomic DNA. Translation: ACZ22199.1.
RefSeq	YP_003315033.1. NC_013521.1.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ACZ22199; ACZ22199; Sked_22840.
GeneID	8633919.
KEGG	ske:Sked_22840.
PATRIC	32393020. VBISanKed123513_2253.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HOG000268120.
KO	K01929.
OMA	GNRNNEI.
Enzyme and pathway databases
BioCyc	SKED446469:GHD3-2280-MONOMER.
UniPathway	UPA00219.
Family and domain databases
Gene3D	3.40.1190.10. 1 hit. 3.90.190.20. 1 hit.
InterPro	IPR004101. Mur_ligase_C. IPR013221. Mur_ligase_cen. IPR000713. Mur_ligase_N. IPR005863. UDP-N-AcMur-pentapeptide_synth. [Graphical view]
PANTHER	PTHR23135:SF3. PTHR23135:SF3. 1 hit.
Pfam	PF01225. Mur_ligase. 1 hit. PF02875. Mur_ligase_C. 1 hit. PF08245. Mur_ligase_M. 1 hit. [Graphical view]
SUPFAM	SSF53244. Mur_ligase_C. 1 hit. SSF53623. Mur_ligase_cen. 1 hit.
TIGRFAMs	TIGR01143. murF. 1 hit.
ProtoNet	Search...

Entry information

Entry name

D1BIM2_SANKS

Accession

Primary (citable) accession number: D1BIM2

Entry history

Integrated into UniProtKB/TrEMBL:	January 19, 2010
Last sequence update:	January 19, 2010
Last modified:	May 1, 2013
This is version 34 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)