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Protein

UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-alanine ligase

Gene

Sked_22840

Organism
Sanguibacter keddieii (strain ATCC 51767 / DSM 10542 / NCFB 3025 / ST-74)
Status
Unreviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Involved in cell wall formation. Catalyzes the final step in the synthesis of UDP-N-acetylmuramoyl-pentapeptide, the precursor of murein.UniRule annotation

Catalytic activityi

ATP + UDP-N-acetylmuramoyl-L-alanyl-gamma-D-glutamyl-L-lysine + D-alanyl-D-alanine = ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-gamma-D-glutamyl-L-lysyl-D-alanyl-D-alanine.UniRule annotation

Pathwayi

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-diaminopimelate-D-alanyl-D-alanine ligase activity Source: InterPro
  3. UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase activity Source: UniProtKB-EC

GO - Biological processi

  1. cell cycle Source: UniProtKB-KW
  2. cell division Source: UniProtKB-KW
  3. cell wall organization Source: UniProtKB-KW
  4. peptidoglycan biosynthetic process Source: UniProtKB-UniPathway
  5. regulation of cell shape Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

LigaseUniRule annotationImported

Keywords - Biological processi

Cell cycle, Cell divisionUniRule annotation, Cell shape, Cell wall biogenesis/degradationUniRule annotation, Peptidoglycan synthesisUniRule annotation

Keywords - Ligandi

ATP-bindingUniRule annotation, Nucleotide-binding

Enzyme and pathway databases

BioCyciSKED446469:GHD3-2280-MONOMER.
UniPathwayiUPA00219.

Names & Taxonomyi

Protein namesi
Recommended name:
UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-alanine ligaseUniRule annotation (EC:6.3.2.10UniRule annotation)
Gene namesi
Ordered Locus Names:Sked_22840Imported
OrganismiSanguibacter keddieii (strain ATCC 51767 / DSM 10542 / NCFB 3025 / ST-74)Imported
Taxonomic identifieri446469 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesMicrococcineaeSanguibacteraceaeSanguibacter
ProteomesiUP000000322: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Family & Domainsi

Sequence similaritiesi

Belongs to the MurCDEF family.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000268120.
KOiK01929.
OMAiGNRNNEI.

Family and domain databases

Gene3Di3.40.1190.10. 1 hit.
3.40.1390.10. 1 hit.
3.90.190.20. 1 hit.
InterProiIPR004101. Mur_ligase_C.
IPR013221. Mur_ligase_cen.
IPR000713. Mur_ligase_N.
IPR005863. UDP-N-AcMur-pentapeptide_synth.
[Graphical view]
PANTHERiPTHR23135:SF3. PTHR23135:SF3. 1 hit.
PfamiPF01225. Mur_ligase. 1 hit.
PF02875. Mur_ligase_C. 1 hit.
PF08245. Mur_ligase_M. 1 hit.
[Graphical view]
SUPFAMiSSF53244. SSF53244. 1 hit.
SSF53623. SSF53623. 1 hit.
SSF63418. SSF63418. 1 hit.
TIGRFAMsiTIGR01143. murF. 1 hit.

Sequencei

Sequence statusi: Complete.

D1BIM2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIDLTAAEVA DLTGGTLAAP HTTRVHGPTV VDSRKVSAGA LFVALVGESS
60 70 80 90 100
DGHDHAAGAV ADGAALVLAS RELDGLPCVV VDDVTRALGL VAAEHLRRLR
110 120 130 140 150
ASGPLQVAAV TGSVGKTTTK DLLGHVLSES GPTVMPRASF NNEIGLPLTV
160 170 180 190 200
LEAGPETRYL VLEMGASAPG DLTYLTGIAP PDVSIVLAVG AAHLGGFGGL
210 220 230 240 250
DGVARAKSEI VTGLRPEGVA VLNADDHRVV AMRDLAPSRV VTFGTVRDAG
260 270 280 290 300
VRAHRVSVDR LGRVSCQVVV GERTADLSLR LVGEHHLTNA LAALTAAVEL
310 320 330 340 350
GVDLDTAAAL VSSVDAASPH RMAVTERPDG VTVIDDSYNA NPDSMRAALK
360 370 380 390 400
ALAVVAGRDR RSVAVLGEML ELGPDSRDQH DEIGRLVVRL NIKLLVVVGA
410 420 430 440 450
GASGLADGAT QEGSWGEEVV EVEDVPAAER FLAEELRSGD VVLVKSSNGS
460
GLWRLGDALT AVTS
Length:464
Mass (Da):47,650
Last modified:January 19, 2010 - v1
Checksum:i1F2A4BC78AFF2AB1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001819 Genomic DNA. Translation: ACZ22199.1.
RefSeqiYP_003315033.1. NC_013521.1.

Genome annotation databases

EnsemblBacteriaiACZ22199; ACZ22199; Sked_22840.
GeneIDi8633919.
KEGGiske:Sked_22840.
PATRICi32393020. VBISanKed123513_2253.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001819 Genomic DNA. Translation: ACZ22199.1.
RefSeqiYP_003315033.1. NC_013521.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACZ22199; ACZ22199; Sked_22840.
GeneIDi8633919.
KEGGiske:Sked_22840.
PATRICi32393020. VBISanKed123513_2253.

Phylogenomic databases

HOGENOMiHOG000268120.
KOiK01929.
OMAiGNRNNEI.

Enzyme and pathway databases

UniPathwayiUPA00219.
BioCyciSKED446469:GHD3-2280-MONOMER.

Family and domain databases

Gene3Di3.40.1190.10. 1 hit.
3.40.1390.10. 1 hit.
3.90.190.20. 1 hit.
InterProiIPR004101. Mur_ligase_C.
IPR013221. Mur_ligase_cen.
IPR000713. Mur_ligase_N.
IPR005863. UDP-N-AcMur-pentapeptide_synth.
[Graphical view]
PANTHERiPTHR23135:SF3. PTHR23135:SF3. 1 hit.
PfamiPF01225. Mur_ligase. 1 hit.
PF02875. Mur_ligase_C. 1 hit.
PF08245. Mur_ligase_M. 1 hit.
[Graphical view]
SUPFAMiSSF53244. SSF53244. 1 hit.
SSF53623. SSF53623. 1 hit.
SSF63418. SSF63418. 1 hit.
TIGRFAMsiTIGR01143. murF. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 51767 / DSM 10542 / NCFB 3025 / ST-74Imported.

Entry informationi

Entry nameiD1BIM2_SANKS
AccessioniPrimary (citable) accession number: D1BIM2
Entry historyi
Integrated into UniProtKB/TrEMBL: January 19, 2010
Last sequence update: January 19, 2010
Last modified: March 4, 2015
This is version 42 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteomeImported

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.