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D1BDU2 (D1BDU2_SANKS) Unreviewed, UniProtKB/TrEMBL

Last modified January 25, 2012. Version 19. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesSubmitted name:
DNA topoisomerase I EMBL ACZ23163.1
Gene names
Ordered Locus Names:Sked_32680
OrganismSanguibacter keddieii (strain ATCC 51767 / DSM 10542 / NCFB 3025 / ST-74) [Complete proteome] [HAMAP] EMBL ACZ23163.1
Taxonomic identifier446469 [NCBI]
Taxonomic lineageBacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Micrococcineae › Sanguibacteraceae › Sanguibacter

Protein attributes

Sequence length962 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand than undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone By similarity. SAAS SAAS000380

Catalytic activity

ATP-independent breakage of single-stranded DNA, followed by passage and rejoining. SAAS SAAS005733

Sequence similarities

Contains 1 Toprim domain. SAAS SAAS023406

Sequences

Sequence LengthMass (Da)Tools
D1BDU2 [UniParc].

Last modified January 19, 2010. Version 1.
Checksum: FAEFEFC1733A7511

FASTA962104,236
        10         20         30         40         50         60 
MSAGRKLVIV ESPAKARTIA GYLGDDYEVE ASVGHIRDLP QPSEMPADMK KGPFGKFAVN 

        70         80         90        100        110        120 
VDGGFEPYYE VYADKKKKVA ELKRALKNAD ELYLATDEDR EGEAIAWHLL QELKPKVPVH 

       130        140        150        160        170        180 
RMVFHEITRE AINRALENTR EIDGRMVDAQ ETRRILDRLY GYEVSPVLWR KVRQGLSAGR 

       190        200        210        220        230        240 
VQSVATRLVV ERERERMAFV TANYWDVSGD FTTQDGPDAG EWFSAKLVQV DGARVASGRD 

       250        260        270        280        290        300 
FTDAGELKSP QGVVHLDEQA AHALVTALAD QPAAVRSLET KPYTRRPAAP FTTSTLQQEA 

       310        320        330        340        350        360 
SRKLRMSSRQ AMRTAQTLYE NGYITYMRTD SPSLSTQATD AARRQAAELY GPEYVPSSPR 

       370        380        390        400        410        420 
VYASKAKGAQ EAHEAIRPAG DSFRTPAQVS REISGDQFKL YELIWKRTVA SQMGDARGST 

       430        440        450        460        470        480 
ASVRLGVAGT LPAGAPSIGD AGPGSQTDAV FAASGTVITF RGFLAAYEEG RDTDRYDSAD 

       490        500        510        520        530        540 
STGTSKKAEG DARLPQMAEG DAMTSRELSA DGHSTSAPPR FTEASLVKAL EERGIGRPST 

       550        560        570        580        590        600 
YASTISTIQD RGYVLSRGQA LVPSWLAFAV IRLLEEHFGQ LIDYDFTAEM EADLDAIAAG 

       610        620        630        640        650        660 
DKERVAWLTQ FYFGDDTEGS DEGLRQLVED LGDIDAREIN SVPIGEGITL RVGRYGPYIE 

       670        680        690        700        710        720 
DTAAEVAEGE NPPRASVPDE VAPDELTVAK ARELLETQGD GDIELGVDPS TQTTIVAKAG 

       730        740        750        760        770        780 
RYGPYVTELL PEPEFEEGLS AAALKRAKAA LPKPRTASLL KTQSLQTITL DDALQLLSLP 

       790        800        810        820        830        840 
RVVGVDPESG TEITAQNGRY GPYLKRGTDS RTLPSEESMF TVTLAEALEI YAQPKRGRGA 

       850        860        870        880        890        900 
TAAPPLKELG EDPTSQKPIV IKDGRFGAYV TDGTTNRTLP KDITIEAITH EQAVELLAEK 

       910        920        930        940        950        960 
RAKGPVKKTT RATAAKKTPA KKTPAKATAA KSTTARSTTT KTATAKKAPA KKAPAKKAAP 


EA

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Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP001819 Genomic DNA. Translation: ACZ23163.1.
RefSeqYP_003315997.1. NC_013521.1.

3D structure databases

ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID8634901.
GenomeReviewsGene locus Sked_32680 in contig CP001819_GR.
KEGGske:Sked_32680.
PATRIC32394999. VBISanKed123513_3227.

Organism-specific databases

CMRSearch...

Phylogenomic databases

OMASTEPVIG.

Family and domain databases

InterProIPR000380. Topo_IA.
IPR003601. Topo_IA_2.
IPR023406. Topo_IA_AS.
IPR013497. Topo_IA_cen.
IPR013824. Topo_IA_cen_sub1.
IPR013825. Topo_IA_cen_sub2.
IPR023405. Topo_IA_core_domain.
IPR003602. Topo_IA_DNA-bd.
IPR005733. TopoI_bac-type.
IPR006171. Toprim_domain.
[Graphical view]
Gene3DG3DSA:1.10.460.10. Topo_IA_cen_sub1. 2 hits.
G3DSA:2.70.20.10. Topo_IA_cen_sub2. 2 hits.
KOK03168.
PANTHERPTHR11390. Topo_IA. 1 hit.
PfamPF01131. Topoisom_bac. 1 hit.
PF01751. Toprim. 1 hit.
[Graphical view]
PRINTSPR00417. PRTPISMRASEI.
SMARTSM00437. TOP1Ac. 1 hit.
SM00436. TOP1Bc. 1 hit.
SM00493. TOPRIM. 1 hit.
[Graphical view]
SUPFAMSSF56712. Topo_IA_core. 1 hit.
TIGRFAMsTIGR01051. TopA_bact. 1 hit.
PROSITEPS00396. TOPOISOMERASE_I_PROK. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameD1BDU2_SANKS
AccessionPrimary (citable) accession number: D1BDU2
Entry history
Integrated into UniProtKB/TrEMBL: January 19, 2010
Last sequence update: January 19, 2010
Last modified: January 25, 2012
This is version 19 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)
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