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D1BDI5 (D1BDI5_SANKS) Unreviewed, UniProtKB/TrEMBL

Last modified January 25, 2012. Version 17. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Glutamate--tRNA ligase HAMAP MF_00022
EC=6.1.1.17 HAMAP MF_00022
Alternative name(s):
Glutamyl-tRNA synthetase HAMAP MF_00022
Gene names
Name:gltX HAMAP MF_00022
Ordered Locus Names:Sked_11010
OrganismSanguibacter keddieii (strain ATCC 51767 / DSM 10542 / NCFB 3025 / ST-74) [Complete proteome] [HAMAP] EMBL ACZ21047.1
Taxonomic identifier446469 [NCBI]
Taxonomic lineageBacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Micrococcineae › Sanguibacteraceae › Sanguibacter

Protein attributes

Sequence length509 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) By similarity. HAMAP MF_00022

Catalytic activity

ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu). HAMAP MF_00022

Subunit structure

Monomer By similarity. HAMAP MF_00022

Subcellular location

Cytoplasm By similarity HAMAP MF_00022.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family. HAMAP MF_00022

Ontologies

Keywords
   Biological processProtein biosynthesis HAMAP MF_00022
   Cellular componentCytoplasm HAMAP MF_00022
   LigandATP-binding HAMAP MF_00022
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase HAMAP MF_00022 EMBL ACZ21047.1
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological processglutamyl-tRNA aminoacylation

Inferred from electronic annotation. Source: HAMAP

   Cellular componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functionATP binding

Inferred from electronic annotation. Source: HAMAP

glutamate-tRNA ligase activity

Inferred from electronic annotation. Source: HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Regions

Motif26 – 3611"HIGH" region By similarity HAMAP MF_00022
Motif270 – 2745"KMSKS" region By similarity HAMAP MF_00022

Sites

Binding site2731ATP By similarity HAMAP MF_00022

Sequences

Sequence LengthMass (Da)Tools
D1BDI5 [UniParc].

Last modified January 19, 2010. Version 1.
Checksum: F9379AB79B7E81AA

FASTA50956,107
        10         20         30         40         50         60 
MYVSVETTPA AVATPSGSAV RVRFCPSPTG TPHVGLIRTA LFNWAYARHT GGTFVFRIED 

        70         80         90        100        110        120 
TDAERDSEES YQQLLDALRW LGLDWDEGIE VGGPHEPYRQ SQRSDIYQDV IRRLVEGGYA 

       130        140        150        160        170        180 
YESFTTPEET EARNVAAGRP KVMGYDGFDR DLTDEQKAGF RAEGREPVLR LRMPDEDVTV 

       190        200        210        220        230        240 
TDLVRGDVTF KAGTVPDYVI VRANGQPLYT LVNPVDDALM EITHVLRGED LLSSTPRQVV 

       250        260        270        280        290        300 
LYRALLEIGV AKVVPAFGHL PYVMGEGNKK LSKRDPESNL FLHRERGFTP EGLLNYLALL 

       310        320        330        340        350        360 
GWSISADNDI FTVDELVAAF DVADVLPNPA RFDVKKAEAI NATHLRRLDG DDFRNRLVPY 

       370        380        390        400        410        420 
LHAAGLVPAD SYADLAPEVQ ALLDAGAPLV QERMTLLGEA PGMLGFLFVA DDAVVVEEAA 

       430        440        450        460        470        480 
RTALREEAAE VLDRSIATLE ALEDFGPTAQ QDALKAELVE AMGVKPKFAF APLRVAITGR 

       490        500 
RVSPPLFESM EILGKERSLV RLRALRASL

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP001819 Genomic DNA. Translation: ACZ21047.1.
RefSeqYP_003313881.1. NC_013521.1.

3D structure databases

ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID8632737.
GenomeReviewsGene locus Sked_11010 in contig CP001819_GR.
KEGGske:Sked_11010.
PATRIC32390636. VBISanKed123513_1081.

Organism-specific databases

CMRSearch...

Phylogenomic databases

OMAMAHIPLI.

Family and domain databases

HAMAPMF_00022_B. Glu_tRNA_synth_B.
[Tree]
InterProIPR008925. aa-tRNA-synth_I_codon-bd.
IPR020751. aa-tRNA-synth_I_codon-bd_sub2.
IPR004527. Glu-tRNA-synth_Ib_bac/mito.
IPR000924. Glu/Gln-tRNA-synth_Ib.
IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
Gene3DG3DSA:1.10.1160.10. Glu/Gln-tRNA-synth_Ic_a-bdl. 1 hit.
G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 2 hits.
G3DSA:1.10.10.350. tRNA_synt_bd. 1 hit.
KOK01885.
PANTHERPTHR10119. Glu_tRNA-synt_1c. 1 hit.
PTHR10119:SF1. PTHR10119:SF1. 1 hit.
PfamPF00749. tRNA-synt_1c. 1 hit.
[Graphical view]
PRINTSPR00987. TRNASYNTHGLU.
SUPFAMSSF48163. tRNA-synt_bind. 1 hit.
TIGRFAMsTIGR00464. GltX_bact. 1 hit.
ProtoNetSearch...

Entry information

Entry nameD1BDI5_SANKS
AccessionPrimary (citable) accession number: D1BDI5
Entry history
Integrated into UniProtKB/TrEMBL: January 19, 2010
Last sequence update: January 19, 2010
Last modified: January 25, 2012
This is version 17 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)
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