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D0ZKZ7 (D0ZKZ7_SALT1) Unreviewed, UniProtKB/TrEMBL

Last modified May 1, 2013. Version 28. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
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Protein attributes

Sequence length572 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction: proline is first activated by ATP to form Pro-AMP and then transferred to the acceptor end of tRNA(Pro). As ProRS can inadvertently accommodate and process non-cognate amino acids such as alanine and cysteine, to avoid such errors it has two additional distinct editing activities against alanine. One activity is designated as 'pretransfer' editing and involves the tRNA(Pro)-independent hydrolysis of activated Ala-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Ala-tRNA(Pro). The misacylated Cys-tRNA(Pro) is not edited by ProRS By similarity. HAMAP-Rule MF_01569 SAAS SAAS023717

Catalytic activity

ATP + L-proline + tRNA(Pro) = AMP + diphosphate + L-prolyl-tRNA(Pro). HAMAP-Rule MF_01569 SAAS SAAS023717

Subunit structure

Homodimer By similarity. HAMAP-Rule MF_01569 SAAS SAAS023717

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_01569 SAAS SAAS023717.

Domain

Consists of three domains: the N-terminal catalytic domain, the editing domain and the C-terminal anticodon-binding domain By similarity. HAMAP-Rule MF_01569

Sequence similarities

Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 1 subfamily. HAMAP-Rule MF_01569

Sequences

Sequence LengthMass (Da)Tools
D0ZKZ7 [UniParc].

Last modified January 19, 2010. Version 1.
Checksum: DCD34C0EFDE33CEC

FASTA57263,540
        10         20         30         40         50         60 
MRTSQYLLST LKETPADAEV ISHQLMLRAG MIRKLASGLY TWLPTGLRVL KKVENIVREE 

        70         80         90        100        110        120 
MNNAGAIEVS MPVVQPADLW QESGRWEQYG PELLRFVDRG ERPFVLGPTH EEVITDLVRN 

       130        140        150        160        170        180 
ELSSYKQLPL NFFQIQTKFR DEVRPRFGVM RSREFLMKDA YSFHTSQESL QETYDAMYAA 

       190        200        210        220        230        240 
YSRIFSRMGL DFRAVQADTG SIGGNASHEF QVLAQSGEDD IVFSDVSDYA ANIELAEAIA 

       250        260        270        280        290        300 
PQTPRAAATQ EMTLVDTPNA KTIAELVEQF NLPIEKTVKT LLVKAVKDSK SPLVALLVRG 

       310        320        330        340        350        360 
DHELNEVKAE KLPHVASPLT FATEEEIRAV INAGPGSLGP VNMPIPVIID RTVAAMSDFA 

       370        380        390        400        410        420 
AGANIDGKHY FGINWDRDVA TPVVADIRNV VAGDPSPDGQ GTLLIKRGIE VGHIFQLGTK 

       430        440        450        460        470        480 
YSEALKASVQ GEDGRNQILT MGCYGIGVTR VVAAAIEQNF DERGIVWPDA IAPFQVAILP 

       490        500        510        520        530        540 
MNMHKSFRVQ ELAEKLYSEL RAQGIEVLMD DRKERPGVMF ADMELIGIPH TIVIGDRNLD 

       550        560        570 
NDDIEYKYRR SGEKSLIKTG DIVDYLVKAI KG

« Hide

References

[1]"Short-term Signatures of Evolutionary Change in the Salmonella enterica serovar Typhimurium 14028 Genome."
Jarvik T., Smillie C., Groisman E.A., Ochman H.
J. Bacteriol. 192:560-567(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 14028s / SGSC 2262.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP001363 Genomic DNA. Translation: ACY86817.1.
RefSeqYP_005235913.1. NC_016856.1.

3D structure databases

ProteinModelPortalD0ZKZ7.
ModBaseSearch...

Proteomic databases

PRIDED0ZKZ7.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaACY86817; ACY86817; STM14_0284.
GeneID11758406.
KEGGseo:STM14_0284.
PATRIC36731788. VBISalEnt101322_0392.

Organism-specific databases

CMRSearch...

Phylogenomic databases

KOK01881.

Family and domain databases

Gene3D3.40.50.800. 1 hit.
3.90.960.10. 1 hit.
HAMAPMF_01569. Pro_tRNA_synth_type1.
InterProIPR002314. aa-tRNA-synt_IIb_cons-dom.
IPR006195. aa-tRNA-synth_II.
IPR004154. Anticodon-bd.
IPR002316. Pro-tRNA-ligase_IIa.
IPR004500. Pro-tRNA-synth_IIa_bac-type.
IPR023717. Pro-tRNA-Synthase_IIa_type1.
IPR007214. YbaK/aa-tRNA-synth-assoc-dom.
[Graphical view]
PANTHERPTHR11451:SF3. PTHR11451:SF3. 1 hit.
PfamPF03129. HGTP_anticodon. 1 hit.
PF00587. tRNA-synt_2b. 1 hit.
PF04073. YbaK. 1 hit.
[Graphical view]
PRINTSPR01046. TRNASYNTHPRO.
SUPFAMSSF52954. Anticodon_bd. 1 hit.
SSF55826. YbaK/aa-tRNA-synth-assoc-reg. 1 hit.
TIGRFAMsTIGR00409. proS_fam_II. 1 hit.
PROSITEPS50862. AA_TRNA_LIGASE_II. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameD0ZKZ7_SALT1
AccessionPrimary (citable) accession number: D0ZKZ7
Entry history
Integrated into UniProtKB/TrEMBL: January 19, 2010
Last sequence update: January 19, 2010
Last modified: May 1, 2013
This is version 28 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)
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