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D0R3X8 (D0R3X8_LACJF) Unreviewed, UniProtKB/TrEMBL

Last modified May 1, 2013. Version 25. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Dihydroorotase HAMAP-Rule MF_00220
Short name=DHOase HAMAP-Rule MF_00220
EC=3.5.2.3 HAMAP-Rule MF_00220
Gene names
Name:pyrC HAMAP-Rule MF_00220 EMBL CAX66791.1
Ordered Locus Names:FI9785_921 EMBL CAX66791.1
OrganismLactobacillus johnsonii (strain FI9785) [Complete proteome] [HAMAP] EMBL CAX66791.1
Taxonomic identifier633699 [NCBI]
Taxonomic lineageBacteria › Firmicutes › Bacilli › Lactobacillales › Lactobacillaceae › Lactobacillus ›

Protein attributes

Sequence length425 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

(S)-dihydroorotate + H2O = N-carbamoyl-L-aspartate. HAMAP-Rule MF_00220

Cofactor

Binds 2 zinc ions per subunit By similarity. HAMAP-Rule MF_00220 SAAS SAAS004722

Pathway

Pyrimidine metabolism; UMP biosynthesis via de novo pathway; (S)-dihydroorotate from bicarbonate: step 3/3. HAMAP-Rule MF_00220 SAAS SAAS002195

Subunit structure

Homodimer By similarity. HAMAP-Rule MF_00220 SAAS SAAS002195

Sequence similarities

Belongs to the DHOase family. Type 2 subfamily. HAMAP-Rule MF_00220

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Sites

Metal binding561Zinc 1 By similarity HAMAP-Rule MF_00220
Metal binding581Zinc 1 By similarity HAMAP-Rule MF_00220
Metal binding1381Zinc 1; via carbamate group By similarity HAMAP-Rule MF_00220
Metal binding1381Zinc 2; via carbamate group By similarity HAMAP-Rule MF_00220
Metal binding1751Zinc 2 By similarity HAMAP-Rule MF_00220
Metal binding2281Zinc 2 By similarity HAMAP-Rule MF_00220
Metal binding3011Zinc 1 By similarity HAMAP-Rule MF_00220

Amino acid modifications

Modified residue1381N6-carboxylysine By similarity HAMAP-Rule MF_00220

Sequences

Sequence LengthMass (Da)Tools
D0R3X8 [UniParc].

Last modified December 15, 2009. Version 1.
Checksum: 73F247BD115CFBE6

FASTA42546,169
        10         20         30         40         50         60 
MTTVIKNGTV YQNGHLIKAD VLIDGQKIKS IGTDLEGEEI IDASGMIVSP GLVDVHVHYR 

        70         80         90        100        110        120 
DPGQTYKEDI KTGSQAAARG GFTTVGAMPN VTPVPNTAEL MEKMVRENQK KGLVHILQYG 

       130        140        150        160        170        180 
PVTKDETTDI IPDYAELKKA GAFALSNDGH GVQSAQTMYL AMQKAKENNL IIAAHAQDDS 

       190        200        210        220        230        240 
LFNKGIVNEG IAAEKLDLPP VTELAETTQI SRDLLLAQKT GVHYHICHVS TKTSVELVRL 

       250        260        270        280        290        300 
AKARGIKVTC EVAPHHILLT DSDIPEDDPY FKMNPPLRSK EDQAALLVGM LDGTIDLIAT 

       310        320        330        340        350        360 
DHAPHAKAEK EGSMRGAAFG ITGSETAFST LYTRFVKEEK VFTLEQLLSL LTDKPATVFG 

       370        380        390        400        410        420 
IKSAGLLAPG ENADIAIFDI EHQKEIKEED FKSKGVNTPF TGQKVYGETV MTFVDGKVVY 


QRGTK

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References

[1]"Complete genome sequence of Lactobacillus johnsonii FI9785, a competitive exclusion agent against pathogens in poultry."
Wegmann U., Overweg K., Horn N., Goesmann A., Narbad A., Gasson M.J., Shearman C.
J. Bacteriol. 191:7142-7143(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: FI9785 EMBL CAX66791.1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		FN298497 Genomic DNA. Translation: CAX66791.1.
RefSeqYP_003293058.1. NC_013504.1.

3D structure databases

ProteinModelPortalD0R3X8.
ModBaseSearch...

Protein-protein interaction databases

STRING633699.FI9785_921.

Protein family/group databases

MEROPSM38.972.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAX66791; CAX66791; FI9785_921.
GeneID8571244.
KEGGljf:FI9785_921.
PATRIC32246433. VBILacJoh137736_0848.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0044.
HOGENOMHOG000219142.
KOK01465.

Enzyme and pathway databases

BioCycLJOH633699:GJ95-859-MONOMER.
UniPathwayUPA00070; UER00117.

Family and domain databases

HAMAPMF_00220_B. PyrC_type2_B.
InterProIPR006680. Amidohydro_1.
IPR004722. DHOase.
IPR002195. Dihydroorotase_CS.
IPR011059. Metal-dep_hydrolase_composite.
[Graphical view]
PfamPF01979. Amidohydro_1. 1 hit.
[Graphical view]
SUPFAMSSF51338. Metalo_hydrolase. 1 hit.
TIGRFAMsTIGR00857. pyrC_multi. 1 hit.
PROSITEPS00483. DIHYDROOROTASE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameD0R3X8_LACJF
AccessionPrimary (citable) accession number: D0R3X8
Entry history
Integrated into UniProtKB/TrEMBL: December 15, 2009
Last sequence update: December 15, 2009
Last modified: May 1, 2013
This is version 25 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)
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