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D0NP70

- LIPA_PHYIT

UniProt

D0NP70 - LIPA_PHYIT

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Protein
Lipoyl synthase, mitochondrial
Gene
PITG_14852
Organism
Phytophthora infestans (strain T30-4) (Potato late blight fungus)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives By similarity.UniRule annotation

Catalytic activityi

Protein N(6)-(octanoyl)lysine + 2 sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = protein N(6)-(lipoyl)lysine + 2 (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.UniRule annotation

Cofactori

Binds 2 4Fe-4S clusters per subunit. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi116 – 1161Iron-sulfur 1 (4Fe-4S) By similarity
Metal bindingi121 – 1211Iron-sulfur 1 (4Fe-4S) By similarity
Metal bindingi127 – 1271Iron-sulfur 1 (4Fe-4S) By similarity
Metal bindingi147 – 1471Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi151 – 1511Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi154 – 1541Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity

GO - Molecular functioni

  1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-HAMAP
  2. lipoate synthase activity Source: UniProtKB-HAMAP
  3. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. protein lipoylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

UniPathwayiUPA00538; UER00593.

Names & Taxonomyi

Protein namesi
Recommended name:
Lipoyl synthase, mitochondrial (EC:2.8.1.8)
Alternative name(s):
Lipoate synthase
Short name:
LS
Short name:
Lip-syn
Lipoic acid synthase
Gene namesi
ORF Names:PITG_14852
OrganismiPhytophthora infestans (strain T30-4) (Potato late blight fungus)
Taxonomic identifieri403677 [NCBI]
Taxonomic lineageiEukaryotaStramenopilesOomycetesPeronosporalesPhytophthora
ProteomesiUP000006643: Partially assembled WGS sequence

Subcellular locationi

Mitochondrion Reviewed prediction UniRule annotation

GO - Cellular componenti

  1. mitochondrion Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 1919Mitochondrion Reviewed prediction
Add
BLAST
Chaini20 – 383364Lipoyl synthase, mitochondrialUniRule annotation
PRO_0000398245Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliD0NP70.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

HOGENOMiHOG000235998.
KOiK03644.
OMAiPEEPYNT.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_00206. Lipoyl_synth.
InterProiIPR013785. Aldolase_TIM.
IPR006638. Elp3/MiaB/NifB.
IPR003698. Lipoyl_synth.
IPR007197. rSAM.
[Graphical view]
PANTHERiPTHR10949. PTHR10949. 1 hit.
PfamiPF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFiPIRSF005963. Lipoyl_synth. 1 hit.
SMARTiSM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00510. lipA. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

D0NP70-1 [UniParc]FASTAAdd to Basket

« Hide

MHASTLTRCM RVAQNARCLS TAAASVQVTH SERGARLAAL RERLAEETRQ    50
GPTFAEQALS LEDFAFEADA APGTKPSRKP NASNRKPKWL KAQPTQGANY 100
ERLRKSVKSL GLSTVCEEAK CPNIGECWGG GKDGIATATI MLMGDTCTRG 150
CSFCAVKTSR KPKPLDIEEP NKVAEAIAAW GLDYIVFTSV DRDDYEDLGA 200
GHFAKTVSTL RAKLPEILIE CLTPDFQGHD NLIDQVATSG LDVFAHNMET 250
VERLQRRVRD YRANYKQSLH VLERAKVAAP HLVTKTSLML GVGERNEDLF 300
QTLRDLRNSG VDVVTFGQYL RPSTKHMPVK SYVTPEAFAE WQKVAEQMGF 350
LYVASGPMVR SSYKAGEFFM KNLLKNRKTQ VVA 383
Length:383
Mass (Da):42,420
Last modified:December 15, 2009 - v1
Checksum:i6D29FBE4DC34315F
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
DS028150 Genomic DNA. Translation: EEY62412.1.
RefSeqiXP_002899048.1. XM_002899002.1.
UniGeneiPin.6624.

Genome annotation databases

EnsemblProtistsiPITG_14852T0; PITG_14852T0; PITG_14852.
GeneIDi9467967.
KEGGipif:PITG_14852.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
DS028150 Genomic DNA. Translation: EEY62412.1 .
RefSeqi XP_002899048.1. XM_002899002.1.
UniGenei Pin.6624.

3D structure databases

ProteinModelPortali D0NP70.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblProtistsi PITG_14852T0 ; PITG_14852T0 ; PITG_14852 .
GeneIDi 9467967.
KEGGi pif:PITG_14852.

Phylogenomic databases

HOGENOMi HOG000235998.
KOi K03644.
OMAi PEEPYNT.

Enzyme and pathway databases

UniPathwayi UPA00538 ; UER00593 .

Family and domain databases

Gene3Di 3.20.20.70. 1 hit.
HAMAPi MF_00206. Lipoyl_synth.
InterProi IPR013785. Aldolase_TIM.
IPR006638. Elp3/MiaB/NifB.
IPR003698. Lipoyl_synth.
IPR007197. rSAM.
[Graphical view ]
PANTHERi PTHR10949. PTHR10949. 1 hit.
Pfami PF04055. Radical_SAM. 1 hit.
[Graphical view ]
PIRSFi PIRSF005963. Lipoyl_synth. 1 hit.
SMARTi SM00729. Elp3. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR00510. lipA. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Genome sequence and analysis of the Irish potato famine pathogen Phytophthora infestans."
    The Broad Institute Genome Sequencing Platform
    Haas B.J., Kamoun S., Zody M.C., Jiang R.H., Handsaker R.E., Cano L.M., Grabherr M., Kodira C.D., Raffaele S., Torto-Alalibo T., Bozkurt T.O., Ah-Fong A.M., Alvarado L., Anderson V.L., Armstrong M.R., Avrova A., Baxter L., Beynon J.
    , Boevink P.C., Bollmann S.R., Bos J.I., Bulone V., Cai G., Cakir C., Carrington J.C., Chawner M., Conti L., Costanzo S., Ewan R., Fahlgren N., Fischbach M.A., Fugelstad J., Gilroy E.M., Gnerre S., Green P.J., Grenville-Briggs L.J., Griffith J., Grunwald N.J., Horn K., Horner N.R., Hu C.H., Huitema E., Jeong D.H., Jones A.M., Jones J.D., Jones R.W., Karlsson E.K., Kunjeti S.G., Lamour K., Liu Z., Ma L., Maclean D., Chibucos M.C., McDonald H., McWalters J., Meijer H.J., Morgan W., Morris P.F., Munro C.A., O'Neill K., Ospina-Giraldo M., Pinzon A., Pritchard L., Ramsahoye B., Ren Q., Restrepo S., Roy S., Sadanandom A., Savidor A., Schornack S., Schwartz D.C., Schumann U.D., Schwessinger B., Seyer L., Sharpe T., Silvar C., Song J., Studholme D.J., Sykes S., Thines M., van de Vondervoort P.J., Phuntumart V., Wawra S., Weide R., Win J., Young C., Zhou S., Fry W., Meyers B.C., van West P., Ristaino J., Govers F., Birch P.R., Whisson S.C., Judelson H.S., Nusbaum C.
    Nature 461:393-398(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: T30-4.

Entry informationi

Entry nameiLIPA_PHYIT
AccessioniPrimary (citable) accession number: D0NP70
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 5, 2010
Last sequence update: December 15, 2009
Last modified: June 11, 2014
This is version 28 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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