Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

ATP-dependent 6-phosphofructokinase

Gene

pfkA

Organism
Rhodothermus marinus (strain ATCC 43812 / DSM 4252 / R-10) (Rhodothermus obamensis)
Status
Unreviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.UniRule annotation

Catalytic activityi

ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate.UniRule annotation

Cofactori

Mg2+UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei14 – 141ATP; via amide nitrogenUniRule annotation
Metal bindingi113 – 1131Magnesium; catalyticUniRule annotation
Sitei114 – 1141Important for substrate specificity; cannot use PPi as phosphoryl donorUniRule annotation
Active sitei137 – 1371Proton acceptorUniRule annotation
Binding sitei172 – 1721Substrate; shared with dimeric partnerUniRule annotation
Binding sitei232 – 2321SubstrateUniRule annotation
Binding sitei276 – 2761Substrate; shared with dimeric partnerUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi77 – 782ATPUniRule annotation
Nucleotide bindingi112 – 1154ATPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

KinaseUniRule annotation, Transferase

Keywords - Biological processi

GlycolysisUniRule annotationSAAS annotation

Keywords - Ligandi

ATP-bindingUniRule annotation, MagnesiumUniRule annotationSAAS annotation, Metal-bindingUniRule annotationSAAS annotation, Nucleotide-binding

Enzyme and pathway databases

BioCyciRMAR518766:GJJ8-306-MONOMER.
UniPathwayiUPA00109; UER00182.

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent 6-phosphofructokinaseUniRule annotation (EC:2.7.1.11UniRule annotation)
Short name:
ATP-PFKUniRule annotation
Short name:
PhosphofructokinaseUniRule annotation
Alternative name(s):
PhosphohexokinaseUniRule annotation
Gene namesi
Name:pfkAUniRule annotation
Ordered Locus Names:Rmar_0296Imported
OrganismiRhodothermus marinus (strain ATCC 43812 / DSM 4252 / R-10) (Rhodothermus obamensis)Imported
Taxonomic identifieri518766 [NCBI]
Taxonomic lineageiBacteriaBacteroidetesBacteroidetes Order II. Incertae sedisRhodothermaceaeRhodothermus
ProteomesiUP000002221 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotationSAAS annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

CytoplasmUniRule annotationSAAS annotation

Interactioni

Subunit structurei

Homodimer or homotetramer.UniRule annotation

Protein-protein interaction databases

STRINGi518766.Rmar_0296.

Structurei

3D structure databases

ProteinModelPortaliD0MDK8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni135 – 1373Substrate bindingUniRule annotation
Regioni179 – 1813Substrate bindingUniRule annotation
Regioni282 – 2854Substrate bindingUniRule annotation

Sequence similaritiesi

Belongs to the phosphofructokinase type A (PFKA) family. Mixed-substrate PFK group III subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0205.
HOGENOMiHOG000248869.
KOiK00850.
OrthoDBiEOG644ZRM.

Family and domain databases

HAMAPiMF_01976. Phosphofructokinase_III.
InterProiIPR022953. ATP_PFK.
IPR012003. ATP_PFK_prok-type.
IPR015912. Phosphofructokinase_CS.
IPR000023. Phosphofructokinase_dom.
IPR012829. Phosphofructokinase_III.
[Graphical view]
PfamiPF00365. PFK. 1 hit.
[Graphical view]
PIRSFiPIRSF000532. ATP_PFK_prok. 1 hit.
PRINTSiPR00476. PHFRCTKINASE.
SUPFAMiSSF53784. SSF53784. 1 hit.
TIGRFAMsiTIGR02483. PFK_mixed. 1 hit.
PROSITEiPS00433. PHOSPHOFRUCTOKINASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

D0MDK8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPRRLRIGLL TGGGDCPGMN AVIRAVTKSL ILQANAEVIG FEDGYEGLIE
60 70 80 90 100
GRFRTLEFQD VSGILTRGGT ILGTSNRANP FRYYRRGEAD VSAEVIALYR
110 120 130 140 150
ELELDGIVAI GGDGTMTIAH GLSERGLRFI GVPKTIDNDI WGTERTFGFD
160 170 180 190 200
TAVHIATEAI DRLHTTAQSH HRVMICETMG RYAGWIALYA GVAAGADVIL
210 220 230 240 250
IPELPFDVEV VAEVCRERES DGHRFTIIVV AEGARPEGGT FHVRERVPES
260 270 280 290 300
PDPIRLGGIG YELERQLRDR LRSEVRTTVL GHVQRGGTPT AYDRSLASAF
310 320 330 340 350
GAYAAALVQA EQYGCMVALR DGRLTTVPLA EVAGRTRTVP LDHPMLGAAL
360 370
AVGTSLGVRA LTSPLVGAEP TTPLA
Length:375
Mass (Da):40,372
Last modified:December 15, 2009 - v1
Checksum:iB58BD59CD47DBC11
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001807 Genomic DNA. Translation: ACY47201.1.
RefSeqiWP_012842813.1. NC_013501.1.
YP_003289589.1. NC_013501.1.

Genome annotation databases

EnsemblBacteriaiACY47201; ACY47201; Rmar_0296.
KEGGirmr:Rmar_0296.
PATRICi32314609. VBIRhoMar93821_0293.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001807 Genomic DNA. Translation: ACY47201.1.
RefSeqiWP_012842813.1. NC_013501.1.
YP_003289589.1. NC_013501.1.

3D structure databases

ProteinModelPortaliD0MDK8.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi518766.Rmar_0296.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACY47201; ACY47201; Rmar_0296.
KEGGirmr:Rmar_0296.
PATRICi32314609. VBIRhoMar93821_0293.

Phylogenomic databases

eggNOGiCOG0205.
HOGENOMiHOG000248869.
KOiK00850.
OrthoDBiEOG644ZRM.

Enzyme and pathway databases

UniPathwayiUPA00109; UER00182.
BioCyciRMAR518766:GJJ8-306-MONOMER.

Family and domain databases

HAMAPiMF_01976. Phosphofructokinase_III.
InterProiIPR022953. ATP_PFK.
IPR012003. ATP_PFK_prok-type.
IPR015912. Phosphofructokinase_CS.
IPR000023. Phosphofructokinase_dom.
IPR012829. Phosphofructokinase_III.
[Graphical view]
PfamiPF00365. PFK. 1 hit.
[Graphical view]
PIRSFiPIRSF000532. ATP_PFK_prok. 1 hit.
PRINTSiPR00476. PHFRCTKINASE.
SUPFAMiSSF53784. SSF53784. 1 hit.
TIGRFAMsiTIGR02483. PFK_mixed. 1 hit.
PROSITEiPS00433. PHOSPHOFRUCTOKINASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 43812 / DSM 4252 / R-10Imported.

Entry informationi

Entry nameiD0MDK8_RHOM4
AccessioniPrimary (citable) accession number: D0MDK8
Entry historyi
Integrated into UniProtKB/TrEMBL: December 15, 2009
Last sequence update: December 15, 2009
Last modified: May 27, 2015
This is version 43 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteomeImported

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.