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D0LKT8 (D0LKT8_HALO1) Unreviewed, UniProtKB/TrEMBL

Last modified June 11, 2014. Version 28. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
D-alanine--D-alanine ligase HAMAP-Rule MF_00047

EC=6.3.2.4 HAMAP-Rule MF_00047
Alternative name(s):
D-Ala-D-Ala ligase HAMAP-Rule MF_00047
D-alanylalanine synthetase HAMAP-Rule MF_00047
Gene names
Name:ddl HAMAP-Rule MF_00047
Ordered Locus Names:Hoch_4160 EMBL ACY16658.1
OrganismHaliangium ochraceum (strain DSM 14365 / JCM 11303 / SMP-2) [Complete proteome] [HAMAP] EMBL ACY16658.1
Taxonomic identifier502025 [NCBI]
Taxonomic lineageBacteriaProteobacteriaDeltaproteobacteriaMyxococcalesNannocystineaeKofleriaceaeHaliangium

Protein attributes

Sequence length333 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Cell wall formation By similarity. HAMAP-Rule MF_00047 SAAS SAAS011095

Catalytic activity

ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine. HAMAP-Rule MF_00047 SAAS SAAS011095

Cofactor

Binds 2 magnesium or manganese ions per subunit By similarity. HAMAP-Rule MF_00047 SAAS SAAS011095

Pathway

Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP-Rule MF_00047 SAAS SAAS011095

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00047 SAAS SAAS011095.

Sequence similarities

Belongs to the D-alanine--D-alanine ligase family. HAMAP-Rule MF_00047

Contains 1 ATP-grasp domain. HAMAP-Rule MF_00047

Contains ATP-grasp domain. SAAS SAAS011095

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Regions

Domain102 – 299198ATP-grasp By similarity HAMAP-Rule MF_00047
Nucleotide binding130 – 18556ATP By similarity HAMAP-Rule MF_00047

Sites

Metal binding2531Magnesium or manganese 1 By similarity HAMAP-Rule MF_00047
Metal binding2661Magnesium or manganese 1 By similarity HAMAP-Rule MF_00047
Metal binding2661Magnesium or manganese 2 By similarity HAMAP-Rule MF_00047
Metal binding2681Magnesium or manganese 2 By similarity HAMAP-Rule MF_00047

Sequences

Sequence LengthMass (Da)Tools
D0LKT8 [UniParc].

Last modified December 15, 2009. Version 1.
Checksum: 0873D345D18696C9

FASTA33335,989
        10         20         30         40         50         60 
MKSRRIGVLM GGLSSEKAIS LATGDAVMAS LEDRGYDVQK IFVDRDVDMA LRQANIDVAF 

        70         80         90        100        110        120 
IALHGRFGED GCIQGLLETM GIPYTGSGVM ASALAMNKAK SKEMFRLHNL PTPSYYVLNR 

       130        140        150        160        170        180 
VDEHDVLGCH GDFGYPVVVK PLTEGSSVGV SLASTPEELL AACERAFVFD HSVVVERFVE 

       190        200        210        220        230        240 
GMEVSVAVLE DRALGAVEVA SEGPLFDYGA KYTSGATEYI IPPRLSPERY RGVLTQAVRA 

       250        260        270        280        290        300 
HLALDCSGAS RVDMIVSPTG NEYILEVNTL PALAPRSLLP KIAVAAGMDF DDLVEAILLG 

       310        320        330 
ARLGQTRERG ERRGTTRPFS GPERRHVAVV EHH 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP001804 Genomic DNA. Translation: ACY16658.1.
RefSeqYP_003268551.1. NC_013440.1.

3D structure databases

ProteinModelPortalD0LKT8.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING502025.Hoch_4160.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaACY16658; ACY16658; Hoch_4160.
GeneID8546563.
KEGGhoh:Hoch_4160.
PATRIC32198095. VBIHalOch22000_4172.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG1181.
HOGENOMHOG000011592.
KOK01921.
OrthoDBEOG6ND0KB.

Enzyme and pathway databases

BioCycHOCH502025:GI43-4193-MONOMER.
UniPathwayUPA00219.

Family and domain databases

Gene3D3.30.1490.20. 1 hit.
3.30.470.20. 2 hits.
3.40.50.20. 1 hit.
HAMAPMF_00047. Dala_Dala_lig.
InterProIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR000291. D-Ala_lig_Van_CS.
IPR005905. D_ala_D_ala.
IPR011095. Dala_Dala_lig_C.
IPR011127. Dala_Dala_lig_N.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PANTHERPTHR23132. PTHR23132. 1 hit.
PfamPF07478. Dala_Dala_lig_C. 1 hit.
PF01820. Dala_Dala_lig_N. 2 hits.
[Graphical view]
SUPFAMSSF52440. SSF52440. 1 hit.
TIGRFAMsTIGR01205. D_ala_D_alaTIGR. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
PS00843. DALA_DALA_LIGASE_1. 1 hit.
PS00844. DALA_DALA_LIGASE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameD0LKT8_HALO1
AccessionPrimary (citable) accession number: D0LKT8
Entry history
Integrated into UniProtKB/TrEMBL: December 15, 2009
Last sequence update: December 15, 2009
Last modified: June 11, 2014
This is version 28 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)