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D0LDT1

- PSB_GORB4

UniProt

D0LDT1 - PSB_GORB4

Protein

Proteasome subunit beta

Gene

prcB

Organism
Gordonia bronchialis (strain ATCC 25592 / DSM 43247 / JCM 3198 / NCTC 10667) (Rhodococcus bronchialis)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 30 (01 Oct 2014)
      Sequence version 1 (15 Dec 2009)
      Previous versions | rss
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    Functioni

    Component of the proteasome core, a large protease complex with broad specificity involved in protein degradation.UniRule annotation

    Catalytic activityi

    Cleavage of peptide bonds with very broad specificity.UniRule annotation

    Enzyme regulationi

    The formation of the proteasomal ATPase ARC-20S proteasome complex, likely via the docking of the C-termini of ARC into the intersubunit pockets in the alpha-rings, may trigger opening of the gate for substrate entry. Interconversion between the open-gate and close-gate conformations leads to a dynamic regulation of the 20S proteasome proteolysis activity.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei63 – 631NucleophileUniRule annotation

    GO - Molecular functioni

    1. threonine-type endopeptidase activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. modification-dependent protein catabolic process Source: UniProtKB-HAMAP
    2. proteasomal protein catabolic process Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Hydrolase, Protease, Threonine protease

    Enzyme and pathway databases

    BioCyciGBRO526226:GHJF-2496-MONOMER.
    UniPathwayiUPA00997.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Proteasome subunit betaUniRule annotation (EC:3.4.25.1UniRule annotation)
    Alternative name(s):
    20S proteasome beta subunitUniRule annotation
    Proteasome core protein PrcBUniRule annotation
    Gene namesi
    Name:prcBUniRule annotation
    Ordered Locus Names:Gbro_2463
    OrganismiGordonia bronchialis (strain ATCC 25592 / DSM 43247 / JCM 3198 / NCTC 10667) (Rhodococcus bronchialis)
    Taxonomic identifieri526226 [NCBI]
    Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeGordoniaceaeGordonia
    ProteomesiUP000001219: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell
    2. proteasome core complex, beta-subunit complex Source: UniProtKB-HAMAP

    Keywords - Cellular componenti

    Cytoplasm, Proteasome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Propeptidei1 – 6262Removed in mature form; by autocatalysisUniRule annotationPRO_0000397512Add
    BLAST
    Chaini63 – 292230Proteasome subunit betaPRO_0000397513Add
    BLAST

    Keywords - PTMi

    Autocatalytic cleavage, Zymogen

    Interactioni

    Subunit structurei

    The 20S proteasome core is composed of 14 alpha and 14 beta subunits that assemble into four stacked heptameric rings, resulting in a barrel-shaped structure. The two inner rings, each composed of seven catalytic beta subunits, are sandwiched by two outer rings, each composed of seven alpha subunits. The catalytic chamber with the active sites is on the inside of the barrel. Has a gated structure, the ends of the cylinder being occluded by the N-termini of the alpha-subunits. Is capped by the proteasome-associated ATPase, ARC.UniRule annotation

    Protein-protein interaction databases

    STRINGi526226.Gbro_2463.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the peptidase T1B family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0638.
    HOGENOMiHOG000245308.
    KOiK03433.
    OMAiFQVELEH.
    OrthoDBiEOG6XM79W.

    Family and domain databases

    Gene3Di3.60.20.10. 1 hit.
    HAMAPiMF_02113_B. Proteasome_B_B.
    InterProiIPR029055. Ntn_hydrolases_N.
    IPR022483. Pept_T1A_Psome_suB_actinobac.
    IPR001353. Proteasome_sua/b.
    IPR023333. Proteasome_suB-type.
    [Graphical view]
    PfamiPF00227. Proteasome. 1 hit.
    [Graphical view]
    SUPFAMiSSF56235. SSF56235. 1 hit.
    TIGRFAMsiTIGR03690. 20S_bact_beta. 1 hit.
    PROSITEiPS51476. PROTEASOME_BETA_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    D0LDT1-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSESLGSVPG SLRSGVISPG LSLGADISSF TEYLRAHAPD QLPQARAEAM    50
    GLHRAADDIP HGTTIVAVSY PGGVILAGDR RATMGNLIAT RDVKKVYITD 100
    EYSAAGIAGT AGIAIEMVRL FAVELEHYEK LEGVPLTLDG KVSRLASMVR 150
    GNLGAALQGL AAVPLLVGYD IDHDDPTERG RIFSFDVAGD RHEEFGGYQA 200
    IGSGSVFAKS SLKKLYRHDL DEASALAIAV EALYDAADDD SATGGPDIVR 250
    KIFPLAAVVD AGGAREVDAE SIEAAARAIV ERRAAEHEGG PR 292
    Length:292
    Mass (Da):30,578
    Last modified:December 15, 2009 - v1
    Checksum:iBC8FA8DC46C6E6A7
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP001802 Genomic DNA. Translation: ACY21704.1.
    RefSeqiWP_012834259.1. NC_013441.1.
    YP_003273597.1. NC_013441.1.

    Genome annotation databases

    EnsemblBacteriaiACY21704; ACY21704; Gbro_2463.
    GeneIDi8551827.
    KEGGigbr:Gbro_2463.
    PATRICi32184537. VBIGorBro114338_2519.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP001802 Genomic DNA. Translation: ACY21704.1 .
    RefSeqi WP_012834259.1. NC_013441.1.
    YP_003273597.1. NC_013441.1.

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 526226.Gbro_2463.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ACY21704 ; ACY21704 ; Gbro_2463 .
    GeneIDi 8551827.
    KEGGi gbr:Gbro_2463.
    PATRICi 32184537. VBIGorBro114338_2519.

    Phylogenomic databases

    eggNOGi COG0638.
    HOGENOMi HOG000245308.
    KOi K03433.
    OMAi FQVELEH.
    OrthoDBi EOG6XM79W.

    Enzyme and pathway databases

    UniPathwayi UPA00997 .
    BioCyci GBRO526226:GHJF-2496-MONOMER.

    Family and domain databases

    Gene3Di 3.60.20.10. 1 hit.
    HAMAPi MF_02113_B. Proteasome_B_B.
    InterProi IPR029055. Ntn_hydrolases_N.
    IPR022483. Pept_T1A_Psome_suB_actinobac.
    IPR001353. Proteasome_sua/b.
    IPR023333. Proteasome_suB-type.
    [Graphical view ]
    Pfami PF00227. Proteasome. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56235. SSF56235. 1 hit.
    TIGRFAMsi TIGR03690. 20S_bact_beta. 1 hit.
    PROSITEi PS51476. PROTEASOME_BETA_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 25592 / DSM 43247 / JCM 3198 / NCTC 10667.

    Entry informationi

    Entry nameiPSB_GORB4
    AccessioniPrimary (citable) accession number: D0LDT1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 10, 2010
    Last sequence update: December 15, 2009
    Last modified: October 1, 2014
    This is version 30 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. Peptidase families
      Classification of peptidase families and list of entries
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3