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Protein

D-inositol 3-phosphate glycosyltransferase

Gene

mshA

Organism
Gordonia bronchialis (strain ATCC 25592 / DSM 43247 / JCM 3198 / NCTC 10667) (Rhodococcus bronchialis)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the transfer of a N-acetyl-glucosamine moiety to 1D-myo-inositol 3-phosphate to produce 1D-myo-inositol 2-acetamido-2-deoxy-glucopyranoside 3-phosphate in the mycothiol biosynthesis pathway.UniRule annotation

Catalytic activityi

UDP-N-acetyl-D-glucosamine + 1D-myo-inositol 3-phosphate = UDP + 1-O-(2-acetamido-2-deoxy-alpha-D-glucopyranosyl)-1D-myo-inositol 3-phosphate.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei16 – 1611D-inositol 3-phosphateUniRule annotation
Binding sitei30 – 301UDP-GlcNAc; via amide nitrogenUniRule annotation
Binding sitei85 – 8511D-inositol 3-phosphateUniRule annotation
Binding sitei118 – 11811D-inositol 3-phosphateUniRule annotation
Binding sitei142 – 14211D-inositol 3-phosphateUniRule annotation
Binding sitei162 – 16211D-inositol 3-phosphateUniRule annotation
Binding sitei236 – 2361UDP-GlcNAcUniRule annotation
Binding sitei241 – 2411UDP-GlcNAcUniRule annotation
Binding sitei302 – 3021UDP-GlcNAc; via amide nitrogen and carbonyl oxygenUniRule annotation
Metal bindingi311 – 3111Magnesium; via carbonyl oxygenUniRule annotation
Metal bindingi312 – 3121Magnesium; via carbonyl oxygenUniRule annotation
Metal bindingi314 – 3141Magnesium; via carbonyl oxygenUniRule annotation
Binding sitei324 – 3241UDP-GlcNAcUniRule annotation
Binding sitei332 – 3321UDP-GlcNAcUniRule annotation
Metal bindingi338 – 3381MagnesiumUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

BioCyciGBRO526226:GHJF-996-MONOMER.

Protein family/group databases

CAZyiGT4. Glycosyltransferase Family 4.

Names & Taxonomyi

Protein namesi
Recommended name:
D-inositol 3-phosphate glycosyltransferase (EC:2.4.1.250UniRule annotation)
Alternative name(s):
N-acetylglucosamine-inositol-phosphate N-acetylglucosaminyltransferaseUniRule annotation
Short name:
GlcNAc-Ins-P N-acetylglucosaminyltransferaseUniRule annotation
Gene namesi
Name:mshAUniRule annotation
Ordered Locus Names:Gbro_0989
OrganismiGordonia bronchialis (strain ATCC 25592 / DSM 43247 / JCM 3198 / NCTC 10667) (Rhodococcus bronchialis)
Taxonomic identifieri526226 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesGordoniaceaeGordonia
Proteomesi
  • UP000001219 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 458458D-inositol 3-phosphate glycosyltransferasePRO_0000400126Add
BLAST

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi526226.Gbro_0989.

Structurei

3D structure databases

ProteinModelPortaliD0L476.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni22 – 232UDP-GlcNAc bindingUniRule annotation
Regioni27 – 3261D-inositol 3-phosphate bindingUniRule annotation

Sequence similaritiesi

Belongs to the glycosyltransferase group 1 family. MshA subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4107R5M. Bacteria.
COG0438. LUCA.
HOGENOMiHOG000077288.
KOiK15521.
OMAiHTMAKVK.
OrthoDBiPOG091H0AO3.

Family and domain databases

HAMAPiMF_01695. MshA. 1 hit.
InterProiIPR001296. Glyco_trans_1.
IPR028098. Glyco_trans_4-like_N.
IPR017814. Mycothiol_biosynthesis_MshA.
[Graphical view]
PfamiPF13439. Glyco_transf_4. 1 hit.
PF00534. Glycos_transf_1. 1 hit.
[Graphical view]
TIGRFAMsiTIGR03449. mycothiol_MshA. 1 hit.

Sequencei

Sequence statusi: Complete.

D0L476-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTTGPLPRRV ALISVHTSPL AQPGTGDAGG MNVYVWQTAT RLARRGVEVE
60 70 80 90 100
IFTRATSSSD APRVAAAPRV TVRNVVAGPF EGLDKRDLPA QLCAFTAGVL
110 120 130 140 150
RAEAAQEPGY YDLIHSHYWL SGQVGWLARD RWGVPLVHTA HTLAAVKNAS
160 170 180 190 200
LAAGDTAEPQ LRVIGEQQVV DEADRLIANT ETEASELISM YGADPARIDV
210 220 230 240 250
VTPGADLDCY TPGPREMART SLGLDQNEAI VTFVGRIQPL KAPDLLIEAA
260 270 280 290 300
APLIRRSRTS RRPVRVLIVG GPSGSGLDRP TALIDLAHDL GIADAVTFLP
310 320 330 340 350
PQAPARLADV YRASNLVAVP SHSESFGLVA IEAQACGTPV LAADVGGLSV
360 370 380 390 400
AVAGGRTGVL VGSHAVGDWT NALEKALAQP DRLAEMGRNA RVHAEQFSWD
410 420 430 440 450
HTVDAMLSSY SRAMQSFAAR NPDAAQSVAA QNVTGSSSRT RRPWRRRRST

LLPMTGRS
Length:458
Mass (Da):48,663
Last modified:December 15, 2009 - v1
Checksum:iC636E4A1499D4566
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001802 Genomic DNA. Translation: ACY20300.1.
RefSeqiWP_012832879.1. NC_013441.1.

Genome annotation databases

EnsemblBacteriaiACY20300; ACY20300; Gbro_0989.
KEGGigbr:Gbro_0989.
PATRICi32181485. VBIGorBro114338_1022.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001802 Genomic DNA. Translation: ACY20300.1.
RefSeqiWP_012832879.1. NC_013441.1.

3D structure databases

ProteinModelPortaliD0L476.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi526226.Gbro_0989.

Protein family/group databases

CAZyiGT4. Glycosyltransferase Family 4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACY20300; ACY20300; Gbro_0989.
KEGGigbr:Gbro_0989.
PATRICi32181485. VBIGorBro114338_1022.

Phylogenomic databases

eggNOGiENOG4107R5M. Bacteria.
COG0438. LUCA.
HOGENOMiHOG000077288.
KOiK15521.
OMAiHTMAKVK.
OrthoDBiPOG091H0AO3.

Enzyme and pathway databases

BioCyciGBRO526226:GHJF-996-MONOMER.

Family and domain databases

HAMAPiMF_01695. MshA. 1 hit.
InterProiIPR001296. Glyco_trans_1.
IPR028098. Glyco_trans_4-like_N.
IPR017814. Mycothiol_biosynthesis_MshA.
[Graphical view]
PfamiPF13439. Glyco_transf_4. 1 hit.
PF00534. Glycos_transf_1. 1 hit.
[Graphical view]
TIGRFAMsiTIGR03449. mycothiol_MshA. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiMSHA_GORB4
AccessioniPrimary (citable) accession number: D0L476
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 2, 2010
Last sequence update: December 15, 2009
Last modified: September 7, 2016
This is version 42 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.