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D0KZL6 (D0KZL6_HALNC) Unreviewed, UniProtKB/TrEMBL

Last modified May 1, 2013. Version 28. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Methionine--tRNA ligase HAMAP-Rule MF_00098
EC=6.1.1.10 HAMAP-Rule MF_00098
Alternative name(s):
Methionyl-tRNA synthetase HAMAP-Rule MF_00098
Gene names
Name:metG HAMAP-Rule MF_00098
Ordered Locus Names:Hneap_1053
OrganismHalothiobacillus neapolitanus (strain ATCC 23641 / c2) (Thiobacillus neapolitanus) [Complete proteome] [HAMAP]
Taxonomic identifier555778 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Gammaproteobacteria › Chromatiales › Halothiobacillaceae › Halothiobacillus ›

Protein attributes

Sequence length685 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation By similarity. HAMAP-Rule MF_00098 SAAS SAAS023458

Catalytic activity

ATP + L-methionine + tRNA(Met) = AMP + diphosphate + L-methionyl-tRNA(Met). HAMAP-Rule MF_00098 SAAS SAAS023458

Cofactor

Binds 1 zinc ion per subunit By similarity. HAMAP-Rule MF_00098 SAAS SAAS023458

Subunit structure

Homodimer By similarity. HAMAP-Rule MF_00098 SAAS SAAS023458

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00098 SAAS SAAS023458.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family. MetG type 1 subfamily. HAMAP-Rule MF_00098

Contains 1 tRNA-binding domain. HAMAP-Rule MF_00098 SAAS SAAS023458

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Regions

Domain583 – 685103tRNA-binding By similarity HAMAP-Rule MF_00098
Motif16 – 2611"HIGH" region By similarity HAMAP-Rule MF_00098
Motif332 – 3365"KMSKS" region By similarity HAMAP-Rule MF_00098

Sites

Metal binding1471Zinc By similarity HAMAP-Rule MF_00098
Metal binding1501Zinc By similarity HAMAP-Rule MF_00098
Metal binding1601Zinc By similarity HAMAP-Rule MF_00098
Metal binding1631Zinc By similarity HAMAP-Rule MF_00098
Binding site3351ATP By similarity HAMAP-Rule MF_00098

Sequences

Sequence LengthMass (Da)Tools
D0KZL6 [UniParc].

Last modified December 15, 2009. Version 1.
Checksum: EDCFF8C411D354DA

FASTA68576,615
        10         20         30         40         50         60 
MTLQAPRRIL VTSALPYANG PLHLGHIIET IQTDIWVRAQ KLFGNECRYL CADDTHGTPI 

        70         80         90        100        110        120 
MLKAQQEGIT PEELITRIGN SHKADFADFM IGFDIFHSTH SEENRRMASE IFKRLDARGL 

       130        140        150        160        170        180 
ISRKTIKQAY DPEKEMFLPD RFIKGECPKC GALDQYGDNC EKCGATYSPT ELKNPRSVLS 

       190        200        210        220        230        240 
GATPVERDSE HYFFDLPKMQ DRLKQWLSED RVQPAIRAKL QEWFDSGLQA WDISRDAPYF 

       250        260        270        280        290        300 
GFEIPGAPGK FFYVWLDAPI GYIGAFLKLA ESTGLDFDEY WGENAQTELI HFIGKDIAYF 

       310        320        330        340        350        360 
HTLFWPATLM GAGLRTPTAV YAHGFLTLNG EKMSKSRGTF IQARTYLNHL DPQALRYYFA 

       370        380        390        400        410        420 
AKLSSGIDDI DLSLEDFRLR VNSDLVGKVV NIASRCAGFI HKQFNSELSA ELAHSELHEQ 

       430        440        450        460        470        480 
LVAAAESIKT RYERRETGQA MREIMALADL VNQYIDEKKP WAMAKSPDSL AAVQGVCTDG 

       490        500        510        520        530        540 
LNAFRILMTY LTPVLPAMSA KAAQFLNLAE FRWSDLARPM VGHKINAFEP LMTRIEQTAI 

       550        560        570        580        590        600 
DALIKATQEE AQAMNQANTT PLTEVKAPRA EIEPIAEEIT IDQFTAVDLR VARIVNAEHV 

       610        620        630        640        650        660 
EGAAKLLRLT LDIDEEKTRQ VFAGIKSAYD PATLVGRHTV MVANLKPRQM KFGLSEGMVL 

       670        680 
AAGDGKGGLF ILSPDEGATA GMRVK

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References

[1]"Complete sequence of Halothiobacillus neapolitanus c2."
US DOE Joint Genome Institute
Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Tice H., Bruce D., Goodwin L., Pitluck S., Davenport K., Brettin T., Detter J.C., Han C., Tapia R., Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N. expand/collapse author list , Kerfeld C., Cannon G., Heinhort S.
Submitted (OCT-2009) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 23641 / c2.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP001801 Genomic DNA. Translation: ACX95889.1.
RefSeqYP_003262936.1. NC_013422.1.

3D structure databases

ProteinModelPortalD0KZL6.
ModBaseSearch...

Protein-protein interaction databases

STRING555778.Hneap_1053.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaACX95889; ACX95889; Hneap_1053.
GeneID8534200.
KEGGhna:Hneap_1053.
PATRIC32205972. VBIHalNea120669_1062.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0143.
HOGENOMHOG000200400.
KOK01874.
OMAHEIAPFK.

Enzyme and pathway databases

BioCycHNEA555778:GIVV-1088-MONOMER.

Family and domain databases

Gene3D2.40.50.140. 1 hit.
3.40.50.620. 2 hits.
HAMAPMF_00098. Met_tRNA_synth_type1.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR004495. Met-tRNA-synth_Ia_bsu_C.
IPR023458. Met-tRNA_ligase_1.
IPR014758. Met-tRNA_synth.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR012340. NA-bd_OB-fold.
IPR014729. Rossmann-like_a/b/a_fold.
IPR002547. tRNA-bd_dom.
IPR009080. tRNAsynth_1a_anticodon-bd.
[Graphical view]
PfamPF09334. tRNA-synt_1g. 1 hit.
PF01588. tRNA_bind. 1 hit.
[Graphical view]
PRINTSPR01041. TRNASYNTHMET.
SUPFAMSSF50249. Nucleic_acid_OB. 1 hit.
SSF47323. tRNAsyn_1a_bind. 1 hit.
TIGRFAMsTIGR00398. metG. 1 hit.
TIGR00399. metG_C_term. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
PS50886. TRBD. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameD0KZL6_HALNC
AccessionPrimary (citable) accession number: D0KZL6
Entry history
Integrated into UniProtKB/TrEMBL: December 15, 2009
Last sequence update: December 15, 2009
Last modified: May 1, 2013
This is version 28 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info