D0KQM8 (AGLU_SULS9) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Alpha-glucosidase
EC=3.2.1.20

Alternative name(s):
Maltase

Gene names

Name:	malA
Ordered Locus Names:	Ssol_0793

Organism

Sulfolobus solfataricus (strain 98/2) [Complete proteome] [HAMAP]

Taxonomic identifier

555311 [NCBI]

Taxonomic lineage

cellular organisms › Archaea › Crenarchaeota › Thermoprotei › Sulfolobales › Sulfolobaceae › Sulfolobus › Sulfolobus solfataricus

Protein attributes

Sequence length	700 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Major soluble alpha-glucosidase. Ref.1
Catalytic activity	Hydrolysis of terminal, non-reducing (1->4)-linked alpha-D-glucose residues with release of alpha-D-glucose.
Subcellular location	Cytoplasm.
Induction	Expressed during growth on maltose. Ref.1
Sequence similarities	Belongs to the glycosyl hydrolase 31 family.
Biophysicochemical properties	pH dependence: Optimum pH is 4.5 for maltose hydrolysis, and 5.5 for glucogen hydrolysis. Ref.1

Ontologies

Keywords
Cellular component	Cytoplasm
Molecular function	Glycosidase Hydrolase
Technical term	Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological_process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	carbohydrate binding Inferred from electronic annotation. Source: InterPro maltose alpha-glucosidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 700	700	Alpha-glucosidase		PRO_0000391339
Sites
	Active site	320	1	By similarity
	Active site	323	1	By similarity
	Active site	416	1	Proton donor By similarity
Experimental info
	Sequence conflict	694 – 700	7	Missing in AAC38215. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	D0KQM8 [UniParc]. Last modified December 15, 2009. Version 1. Checksum: 8F329E799CA542B7	FASTA	700	81,141
	10 20 30 40 50 60 MQTIKIYENK GVYKVVIGEP FPPIEFPLEQ KISSNKSLSE LGLTIVQQGN KVIVEKSLDL 70 80 90 100 110 120 KEHIIGLGEK AFELDRKRKR YVMYNVDAGA YKKYQDPLYV SIPLFISVKD GVATGYFFNS 130 140 150 160 170 180 ASKVIFDVGL EEYDKVIVTI PEDSVEFYVI EGPRIEDVLE KYTELTGKPF LPPMWAFGYM 190 200 210 220 230 240 ISRYSYYPQD KVVELVDIMQ KEGFRVAGVF LDIHYMDSYK LFTWHPYRFP EPKKLIDELH 250 260 270 280 290 300 KRNVKLITIV DHGIRVDQNY SPFLSGMGKF CEIESGELFV GKMWPGTTVY PDFFREDTRE 310 320 330 340 350 360 WWAGLISEWL SQGVDGIWLD MNEPTDFSRA IEIRDVLSSL PVQFRDDRLV TTFPDNVVHY 370 380 390 400 410 420 LRGKRVKHEK VRNAYPLYEA MATFKGFRTS HRNEIFILSR AGYAGIQRYA FIWTGDNTPS 430 440 450 460 470 480 WDDLKLQLQL VLGLSISGVP FVGCDIGGFQ GRNFAEIDNS MDLLVKYYAL ALFFPFYRSH 490 500 510 520 530 540 KATDGIDTEP VFLPDYYKEK VKEIVELRYK FLPYIYSLAL EASEKGHPVI RPLFYEFQDD 550 560 570 580 590 600 DDMYRIEDEY MVGKYLLYAP IVSKEESRLV TLPRGKWYNY WNGEIINGKS VVKSTHELPI 610 620 630 640 650 660 YLREGSIIPL EGDELIVYGE TSFKRYDNAE ITSSSNEIKF SREIYVSKLT ITSEKPVSKI 670 680 690 700 IVDDSKEIQV EKTMQNTYVA KINQKIRGKI NLEGSVLKQS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular characterization of the alpha-glucosidase gene (malA) from the hyperthermophilic archaeon Sulfolobus solfataricus." Rolfsmeier M., Haseltine C., Bini E., Clark A., Blum P. J. Bacteriol. 180:1287-1295(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-20 AND 552-561, FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, INDUCTION.
[2]	"Complete sequence of Sulfolobus solfataricus 98/2." US DOE Joint Genome Institute Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Tice H., Bruce D., Goodwin L., Pitluck S., Munk A.C., Brettin T., Detter J.C., Han C., Tapia R., Larimer F., Land M., Hauser L., Kyrpides N., Ovchinnikova G., Mead D. Submitted (OCT-2009) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 98/2.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF042494 Genomic DNA. Translation: AAC38215.1. CP001800 Genomic DNA. Translation: ACX91047.1.
RefSeq	YP_005642648.1. NC_017274.1.
3D structure databases
ModBase	Search...
Protein family/group databases
CAZy	GH31. Glycoside Hydrolase Family 31.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ACX91047; ACX91047; Ssol_0793.
GeneID	12258172.
KEGG	sol:Ssol_0793.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HOG000033867.
KO	K01187.
OMA	FVITRAA.
Family and domain databases
Gene3D	3.20.20.70. 2 hits.
InterPro	IPR013785. Aldolase_TIM. IPR011013. Gal_mutarotase_SF_dom. IPR000322. Glyco_hydro_31. IPR025887. Glyco_hydro_31_N_dom. IPR017853. Glycoside_hydrolase_SF. [Graphical view]
PANTHER	PTHR22762. PTHR22762. 1 hit.
Pfam	PF13802. Gal_mutarotas_2. 1 hit. PF01055. Glyco_hydro_31. 1 hit. [Graphical view]
SUPFAM	SSF74650. Gal_mut_like. 1 hit. SSF51445. Glyco_hydro_cat. 1 hit.
PROSITE	PS00129. GLYCOSYL_HYDROL_F31_1. 1 hit. PS00707. GLYCOSYL_HYDROL_F31_2. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AGLU_SULS9

Accession

Primary (citable) accession number: D0KQM8
Secondary accession number(s): O59645

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 9, 2010
Last sequence update:	December 15, 2009
Last modified:	May 1, 2013
This is version 26 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program