Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

D0KQM8 (AGLU_SULS9) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 30. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Alpha-glucosidase

EC=3.2.1.20
Alternative name(s):
Maltase
Gene names
Name:malA
Ordered Locus Names:Ssol_0793
OrganismSulfolobus solfataricus (strain 98/2) [Complete proteome] [HAMAP]
Taxonomic identifier555311 [NCBI]
Taxonomic lineageArchaeaCrenarchaeotaThermoproteiSulfolobalesSulfolobaceaeSulfolobus

Protein attributes

Sequence length700 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Major soluble alpha-glucosidase. Ref.1

Catalytic activity

Hydrolysis of terminal, non-reducing (1->4)-linked alpha-D-glucose residues with release of alpha-D-glucose.

Subcellular location

Cytoplasm.

Induction

Expressed during growth on maltose. Ref.1

Sequence similarities

Belongs to the glycosyl hydrolase 31 family.

Biophysicochemical properties

pH dependence:

Optimum pH is 4.5 for maltose hydrolysis, and 5.5 for glucogen hydrolysis. Ref.1

Ontologies

Keywords
   Cellular componentCytoplasm
   Molecular functionGlycosidase
Hydrolase
   Technical termComplete proteome
Direct protein sequencing
Gene Ontology (GO)
   Biological_processcarbohydrate metabolic process

Inferred from electronic annotation. Source: InterPro

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functioncarbohydrate binding

Inferred from electronic annotation. Source: InterPro

maltose alpha-glucosidase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 700700Alpha-glucosidase
PRO_0000391339

Sites

Active site3201 By similarity
Active site3231 By similarity
Active site4161Proton donor By similarity

Experimental info

Sequence conflict694 – 7007Missing in AAC38215. Ref.1

Sequences

Sequence LengthMass (Da)Tools
D0KQM8 [UniParc].

Last modified December 15, 2009. Version 1.
Checksum: 8F329E799CA542B7

FASTA70081,141
        10         20         30         40         50         60 
MQTIKIYENK GVYKVVIGEP FPPIEFPLEQ KISSNKSLSE LGLTIVQQGN KVIVEKSLDL 

        70         80         90        100        110        120 
KEHIIGLGEK AFELDRKRKR YVMYNVDAGA YKKYQDPLYV SIPLFISVKD GVATGYFFNS 

       130        140        150        160        170        180 
ASKVIFDVGL EEYDKVIVTI PEDSVEFYVI EGPRIEDVLE KYTELTGKPF LPPMWAFGYM 

       190        200        210        220        230        240 
ISRYSYYPQD KVVELVDIMQ KEGFRVAGVF LDIHYMDSYK LFTWHPYRFP EPKKLIDELH 

       250        260        270        280        290        300 
KRNVKLITIV DHGIRVDQNY SPFLSGMGKF CEIESGELFV GKMWPGTTVY PDFFREDTRE 

       310        320        330        340        350        360 
WWAGLISEWL SQGVDGIWLD MNEPTDFSRA IEIRDVLSSL PVQFRDDRLV TTFPDNVVHY 

       370        380        390        400        410        420 
LRGKRVKHEK VRNAYPLYEA MATFKGFRTS HRNEIFILSR AGYAGIQRYA FIWTGDNTPS 

       430        440        450        460        470        480 
WDDLKLQLQL VLGLSISGVP FVGCDIGGFQ GRNFAEIDNS MDLLVKYYAL ALFFPFYRSH 

       490        500        510        520        530        540 
KATDGIDTEP VFLPDYYKEK VKEIVELRYK FLPYIYSLAL EASEKGHPVI RPLFYEFQDD 

       550        560        570        580        590        600 
DDMYRIEDEY MVGKYLLYAP IVSKEESRLV TLPRGKWYNY WNGEIINGKS VVKSTHELPI 

       610        620        630        640        650        660 
YLREGSIIPL EGDELIVYGE TSFKRYDNAE ITSSSNEIKF SREIYVSKLT ITSEKPVSKI 

       670        680        690        700 
IVDDSKEIQV EKTMQNTYVA KINQKIRGKI NLEGSVLKQS 

« Hide

References

« Hide 'large scale' references
[1]"Molecular characterization of the alpha-glucosidase gene (malA) from the hyperthermophilic archaeon Sulfolobus solfataricus."
Rolfsmeier M., Haseltine C., Bini E., Clark A., Blum P.
J. Bacteriol. 180:1287-1295(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-20 AND 552-561, FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, INDUCTION.
[2]"Complete sequence of Sulfolobus solfataricus 98/2."
US DOE Joint Genome Institute
Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Tice H., Bruce D., Goodwin L., Pitluck S., Munk A.C., Brettin T., Detter J.C., Han C., Tapia R., Larimer F., Land M., Hauser L., Kyrpides N., Ovchinnikova G., Mead D.
Submitted (OCT-2009) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 98/2.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF042494 Genomic DNA. Translation: AAC38215.1.
CP001800 Genomic DNA. Translation: ACX91047.1.
RefSeqYP_005642648.1. NC_017274.1.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protein family/group databases

CAZyGH31. Glycoside Hydrolase Family 31.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaACX91047; ACX91047; Ssol_0793.
GeneID12258172.
KEGGsol:Ssol_0793.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMHOG000033867.
KOK01187.
OMAMQMVRAT.

Enzyme and pathway databases

BioCycSSOL555311:GLLY-794-MONOMER.

Family and domain databases

Gene3D3.20.20.70. 2 hits.
InterProIPR013785. Aldolase_TIM.
IPR011013. Gal_mutarotase_SF_dom.
IPR000322. Glyco_hydro_31.
IPR025887. Glyco_hydro_31_N_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamPF13802. Gal_mutarotas_2. 1 hit.
PF01055. Glyco_hydro_31. 1 hit.
[Graphical view]
SUPFAMSSF51445. SSF51445. 2 hits.
SSF74650. SSF74650. 1 hit.
PROSITEPS00129. GLYCOSYL_HYDROL_F31_1. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameAGLU_SULS9
AccessionPrimary (citable) accession number: D0KQM8
Secondary accession number(s): O59645
Entry history
Integrated into UniProtKB/Swiss-Prot: February 9, 2010
Last sequence update: December 15, 2009
Last modified: February 19, 2014
This is version 30 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries