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Protein

NAD kinase

Gene

nadK

Organism
Pectobacterium wasabiae (strain WPP163)
Status
Unreviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Involved in the regulation of the intracellular balance of NAD and NADP, and is a key enzyme in the biosynthesis of NADP. Catalyzes specifically the phosphorylation on 2'-hydroxyl of the adenosine moiety of NAD to yield NADP.UniRule annotation

Catalytic activityi

ATP + NAD+ = ADP + NADP+.UniRule annotationSAAS annotation

Cofactori

a divalent metal cationUniRule annotationSAAS annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei79 – 791Proton acceptorUniRule annotation
Binding sitei164 – 1641NADUniRule annotation
Binding sitei181 – 1811NADUniRule annotation
Binding sitei183 – 1831NADUniRule annotation
Binding sitei253 – 2531NADUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi79 – 802NADUniRule annotation
Nucleotide bindingi153 – 1542NADUniRule annotation
Nucleotide bindingi194 – 1996NADUniRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. metal ion binding Source: UniProtKB-HAMAP
  3. NAD+ kinase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. NAD metabolic process Source: InterPro
  2. NADP biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

KinaseUniRule annotationSAAS annotation, Transferase

Keywords - Ligandi

ATP-bindingUniRule annotationSAAS annotation, NADUniRule annotationSAAS annotation, NADPUniRule annotationSAAS annotation, Nucleotide-binding

Enzyme and pathway databases

BioCyciPWAS561231:GHO0-989-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
NAD kinaseUniRule annotationSAAS annotation (EC:2.7.1.23UniRule annotationSAAS annotation)
Alternative name(s):
ATP-dependent NAD kinaseUniRule annotation
Gene namesi
Name:nadKUniRule annotation
Ordered Locus Names:Pecwa_0964Imported
OrganismiPectobacterium wasabiae (strain WPP163)Imported
Taxonomic identifieri561231 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaePectobacterium
ProteomesiUP000000315 Componenti: Chromosome

Subcellular locationi

  1. Cytoplasm UniRule annotationSAAS annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

CytoplasmUniRule annotationSAAS annotation

Interactioni

Protein-protein interaction databases

STRINGi561231.Pecwa_0964.

Family & Domainsi

Sequence similaritiesi

Belongs to the NAD kinase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0061.
HOGENOMiHOG000227221.
KOiK00858.
OMAiGMVELRV.
OrthoDBiEOG6PZXDR.

Family and domain databases

Gene3Di2.60.200.30. 1 hit.
3.40.50.10330. 1 hit.
HAMAPiMF_00361. NAD_kinase.
InterProiIPR017438. ATP-NAD_kinase_dom_1.
IPR017437. ATP-NAD_kinase_PpnK-typ_all-b.
IPR016064. NAD/diacylglycerol_kinase.
IPR002504. NADK.
[Graphical view]
PANTHERiPTHR20275. PTHR20275. 1 hit.
PfamiPF01513. NAD_kinase. 1 hit.
[Graphical view]
SUPFAMiSSF111331. SSF111331. 1 hit.

Sequencei

Sequence statusi: Complete.

D0KIF9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNTPLTMNRP FNCIGIVGHP RHPTALATHE MLYHWLTEKG YSVLIEQQIA
60 70 80 90 100
RELNLKNAPT GSLADIGQQA DLAVVVGGDG NMLGAARVLS RYDIKVIGVN
110 120 130 140 150
RGNLGFLTDL DPDHAQQQLS DVLDGHYLSE QRFMLEAHVC RANQPDSIST
160 170 180 190 200
AINEVVLHPG KVAHMIEFEV YIDDKFAFSQ RSDGLIIATP TGSTAYSLSA
210 220 230 240 250
GGPILTPSLE AIALVPMFPH TLSARPLVIN SSSTIRLKFS CITNDLEISC
260 270 280 290
DSQIALPVQE GEEVLIRRSE HHLNLIHPKN YSYFNTLSSK LGWSKKLF
Length:298
Mass (Da):32,793
Last modified:December 15, 2009 - v1
Checksum:i717EC4F6DB0488B5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001790 Genomic DNA. Translation: ACX86781.1.
RefSeqiYP_003258388.1. NC_013421.1.

Genome annotation databases

EnsemblBacteriaiACX86781; ACX86781; Pecwa_0964.
KEGGipwa:Pecwa_0964.
PATRICi32276116. VBIPecWas23660_0905.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001790 Genomic DNA. Translation: ACX86781.1.
RefSeqiYP_003258388.1. NC_013421.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi561231.Pecwa_0964.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACX86781; ACX86781; Pecwa_0964.
KEGGipwa:Pecwa_0964.
PATRICi32276116. VBIPecWas23660_0905.

Phylogenomic databases

eggNOGiCOG0061.
HOGENOMiHOG000227221.
KOiK00858.
OMAiGMVELRV.
OrthoDBiEOG6PZXDR.

Enzyme and pathway databases

BioCyciPWAS561231:GHO0-989-MONOMER.

Family and domain databases

Gene3Di2.60.200.30. 1 hit.
3.40.50.10330. 1 hit.
HAMAPiMF_00361. NAD_kinase.
InterProiIPR017438. ATP-NAD_kinase_dom_1.
IPR017437. ATP-NAD_kinase_PpnK-typ_all-b.
IPR016064. NAD/diacylglycerol_kinase.
IPR002504. NADK.
[Graphical view]
PANTHERiPTHR20275. PTHR20275. 1 hit.
PfamiPF01513. NAD_kinase. 1 hit.
[Graphical view]
SUPFAMiSSF111331. SSF111331. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: WPP163Imported.

Entry informationi

Entry nameiD0KIF9_PECWW
AccessioniPrimary (citable) accession number: D0KIF9
Entry historyi
Integrated into UniProtKB/TrEMBL: December 15, 2009
Last sequence update: December 15, 2009
Last modified: April 29, 2015
This is version 40 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteomeImported

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.