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D0JB65 (D0JB65_BLASB) Unreviewed, UniProtKB/TrEMBL

Last modified May 1, 2013. Version 26. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
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Protein attributes

Sequence length374 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Exchanges the guanine residue with 7-aminomethyl-7-deazaguanine in tRNAs with GUN anticodons (tRNA-Asp, -Asn, -His and -Tyr). After this exchange, a cyclopentendiol moiety is attached to the 7-aminomethyl group of 7-deazaguanine, resulting in the hypermodified nucleoside queuosine (Q) (7-(((4,5-cis-dihydroxy-2-cyclopenten-1-yl)amino)methyl)-7-deazaguanosine) By similarity. RuleBase RU003777 SAAS SAAS004803 HAMAP-Rule MF_00168

Catalytic activity

Guanine34 in tRNA + 7-aminomethyl-7-carbaguanine = 7-aminomethyl-7-carbaguanine34 in tRNA + guanine. SAAS SAAS004803 HAMAP-Rule MF_00168

Guanine34 in tRNA + queuine = queuosine34 in tRNA + guanine. SAAS SAAS004803 HAMAP-Rule MF_00168

[tRNA]-guanine + queuine = [tRNA]-queuine + guanine. RuleBase RU003777

Cofactor

Binds 1 zinc ion per subunit By similarity. RuleBase RU003777 SAAS SAAS004803 HAMAP-Rule MF_00168

Pathway

tRNA modification; tRNA-queuosine biosynthesis. HAMAP-Rule MF_00168 SAAS SAAS004803

Sequence similarities

Belongs to the queuine tRNA-ribosyltransferase family. RuleBase RU003777 HAMAP-Rule MF_00168

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Sites

Active site901Nucleophile By similarity HAMAP-Rule MF_00168
Metal binding3381Zinc By similarity HAMAP-Rule MF_00168
Binding site911Substrate By similarity HAMAP-Rule MF_00168

Sequences

Sequence LengthMass (Da)Tools
D0JB65 [UniParc].

Last modified December 15, 2009. Version 1.
Checksum: E760518CCAF503D3

FASTA37442,976
        10         20         30         40         50         60 
MKFNLIKTDR FSKARAGILK TDHGKIETPI FMPVASKGYV KSVPIHEIHK ISKIILGNTY 

        70         80         90        100        110        120 
HLYLKPGIEV FHKAGGIHSF MNWNESILTD SGGFQVFSMR KLNKITENGV VFKSIINGSY 

       130        140        150        160        170        180 
HFFSPEKSME IQRFIGGDII MAFDDCPPFP CSYQEAKKSI KKTHIWLKKC CSYLQNHPEI 

       190        200        210        220        230        240 
YNYKQSFFPI LQGSIYPDLR KNSAKEISLL ETEGYAIGGL SLGEEKEQTQ NIIDLVTDIL 

       250        260        270        280        290        300 
PKEKPRYLMG VGNPVDILEG ISLGIDMFDC VIPTRNGRHG MLFTWKGIMN MKNKKWEKDF 

       310        320        330        340        350        360 
SCLDEFGNSY VDQSYSKSYV RHLFLSRDNL AKEIASIHNL SFYSDLIQQA RVHIMNNQFF 

       370 
SWKESVIPLL KKRL

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References

[1]"Evolutionary convergence and nitrogen metabolism in Blattabacterium strain Bge, primary endosymbiont of the cockroach Blattella germanica."
Lopez-Sanchez M.J., Neef A., Pereto J., Patino-Navarrete R., Pignatelli M., Latorre A., Moya A.
PLoS Genet. 5:E1000721-E1000721(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Bge.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP001487 Genomic DNA. Translation: ACY40403.1.
RefSeqYP_003284013.1. NC_013454.1.

3D structure databases

ModBaseSearch...

Protein-protein interaction databases

STRING331104.BLBBGE_394.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaACY40403; ACY40403; BLBBGE_394.
GeneID8554953.
KEGGbbl:BLBBGE_394.
PATRIC31959420. VBIBlaSp132937_0372.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0343.
HOGENOMHOG000223473.
KOK00773.
OMAMSPERSI.

Enzyme and pathway databases

BioCycBSP331104:GH6K-398-MONOMER.
UniPathwayUPA00392.

Family and domain databases

Gene3D3.20.20.105. 1 hit.
HAMAPMF_00168. Q_tRNA_Tgt.
InterProIPR004803. Queuine_tRNA-ribosylTrfase.
IPR002616. tRNA_ribo_trans.
[Graphical view]
PANTHERPTHR11962. PTHR11962. 1 hit.
PfamPF01702. TGT. 1 hit.
[Graphical view]
SUPFAMSSF51713. tRNA_ribo_trans. 1 hit.
TIGRFAMsTIGR00430. Q_tRNA_tgt. 1 hit.
TIGR00449. tgt_general. 1 hit.
ProtoNetSearch...

Entry information

Entry nameD0JB65_BLASB
AccessionPrimary (citable) accession number: D0JB65
Entry history
Integrated into UniProtKB/TrEMBL: December 15, 2009
Last sequence update: December 15, 2009
Last modified: May 1, 2013
This is version 26 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info