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D0JAF7 (D0JAF7_BLASB) Unreviewed, UniProtKB/TrEMBL

Last modified December 14, 2011. Version 15. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase HAMAP MF_00172
EC=2.1.1.14 HAMAP MF_00172
Alternative name(s):
Cobalamin-independent methionine synthase HAMAP MF_00172
Methionine synthase, vitamin-B12 independent isozyme HAMAP MF_00172
Gene names
Name:metE HAMAP MF_00172 EMBL ACY40145.1
Ordered Locus Names:BLBBGE_114
OrganismBlattabacterium sp. subsp. Blattella germanica (strain Bge) (Blattella germanica symbiotic bacterium) [Complete proteome] [HAMAP]
Taxonomic identifier331104 [NCBI]
Taxonomic lineageBacteria › Bacteroidetes › Flavobacteriia › Flavobacteriales › Blattabacteriaceae › Blattabacterium

Protein attributes

Sequence length767 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the transfer of a methyl group from 5-methyltetrahydrofolate to homocysteine resulting in methionine formation By similarity. HAMAP MF_00172 SAAS SAAS013215

Catalytic activity

5-methyltetrahydropteroyltri-L-glutamate + L-homocysteine = tetrahydropteroyltri-L-glutamate + L-methionine. HAMAP MF_00172 SAAS SAAS013215

Cofactor

Binds 1 zinc ion per subunit By similarity. HAMAP MF_00172

Pathway

Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; L-methionine from L-homocysteine (MetE route): step 1/1. HAMAP MF_00172 SAAS SAAS013215

Sequence similarities

Belongs to the vitamin-B12 independent methionine synthase family. HAMAP MF_00172

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Sites

Metal binding6491Zinc By similarity HAMAP MF_00172
Metal binding6511Zinc By similarity HAMAP MF_00172
Metal binding7341Zinc By similarity HAMAP MF_00172

Sequences

Sequence LengthMass (Da)Tools
D0JAF7 [UniParc].

Last modified December 15, 2009. Version 1.
Checksum: D28E4D6EC9149A39

FASTA76789,662
        10         20         30         40         50         60 
MLKHNLGYPR IGIQRELKKA CEAYWSQKID SNALFEVGKK IRKENWKMQE MANLDLIPCN 

        70         80         90        100        110        120 
DFSFYDHVLD MSLLLGAIPE SYLSVPMIQN HIDLYFSMAR GFQKNQWDIK AMEMTKWFNT 

       130        140        150        160        170        180 
NYHYIVPEFD KNQKFSIFSK KIFDELEESK KLLKSIKKIK PVLIGPVSYL FLGKEKEKSF 

       190        200        210        220        230        240 
HRMDLIENLV PTYTKIINEL KNKGVDWIQL DEPILVLDMS EKEKEAFQYA YKEISKSCIG 

       250        260        270        280        290        300 
TNILLTSYFD GILENISLLR DVFVKALHID LVEDSEQLEK ILFFVKESKM ILSLGIIDGR 

       310        320        330        340        350        360 
NIWKNNYTDS IKKIEKTIEF IGEDRVMIAP NCSLLHVPIN IEYEHFIHED IKNRMSFARQ 

       370        380        390        400        410        420 
KIDELSDLEC IIKGNKDILL NNFSLLEKSK KSSIFHDKKI KERATQIKDK DLQRENPFHI 

       430        440        450        460        470        480 
RQKKQKEKFR LPLFPTTTIG SFPQTKEIRR LRNQFRKKEL SKEEYDQKIQ SFIVDVIKKQ 

       490        500        510        520        530        540 
EEVDLDVLVH GEFERTDMVE YFSDKLKGIL STENGWVQSY GSRCVKPPII YGDVSRIVDM 

       550        560        570        580        590        600 
TVDWICFAQS KTKKLMKGML TGPVTILQWS FVRNDQPVSH TAYQIAWSIR EEVLSLEKSG 

       610        620        630        640        650        660 
IQIIQIDEPA LREGLPLKHK NWKSYLDWSI KAFRISSSGV KDETQIHTHM CYSEFNDILE 

       670        680        690        700        710        720 
NIADMDADVI TMETSRSKME LLKAFSIFSY PNEIGPGVYD IHSPRIPTIE EIFDLIEKAS 

       730        740        750        760 
KKLPPRNIWV NPDCGLKTRK WEEVLKSLKN MTDAAKIARL KLANQSS

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References

[1]"Evolutionary convergence and nitrogen metabolism in Blattabacterium strain Bge, primary endosymbiont of the cockroach Blattella germanica."
Lopez-Sanchez M.J., Neef A., Pereto J., Patino-Navarrete R., Pignatelli M., Latorre A., Moya A.
PLoS Genet. 5:E1000721-E1000721(2009) [PubMed: 19911043] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP001487 Genomic DNA. Translation: ACY40145.1.
RefSeqYP_003283755.1. NC_013454.1.

3D structure databases

ProteinModelPortalD0JAF7.
ModBaseSearch...

Protein-protein interaction databases

STRINGD0JAF7.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID8554673.
GenomeReviewsGene locus BLBBGE_114 in contig CP001487_GR.
KEGGbbl:BLBBGE_114.
PATRIC31958858. VBIBlaSp132937_0109.

Organism-specific databases

CMRSearch...

Phylogenomic databases

OMARNIWRAN.

Family and domain databases

HAMAPMF_00172. Meth_synth.
[Tree]
InterProIPR013215. Cbl-indep_Met_Synth_N.
IPR006276. Cobalamin-indep_Met_synthase.
IPR002629. Methionine_synth.
[Graphical view]
KOK00549.
PfamPF08267. Meth_synt_1. 1 hit.
PF01717. Meth_synt_2. 1 hit.
[Graphical view]
PIRSFPIRSF000382. MeTrfase_B12_ind. 1 hit.
TIGRFAMsTIGR01371. Met_syn_B12ind. 1 hit.
ProtoNetSearch...

Entry information

Entry nameD0JAF7_BLASB
AccessionPrimary (citable) accession number: D0JAF7
Entry history
Integrated into UniProtKB/TrEMBL: December 15, 2009
Last sequence update: December 15, 2009
Last modified: December 14, 2011
This is version 15 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info