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Protein

Enolase

Gene

eno

Organism
Blattabacterium sp. subsp. Periplaneta americana (strain BPLAN) (Periplaneta americana symbiotic bacterium)
Status
Unreviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the reversible conversion of 2-phosphoglycerate into phosphoenolpyruvate. It is essential for the degradation of carbohydrates via glycolysis.UniRule annotation

Catalytic activityi

2-phospho-D-glycerate = phosphoenolpyruvate + H2O.UniRule annotation

Cofactori

Mg2+UniRule annotation

Enzyme regulationi

The covalent binding to the substrate causes inactivation of the enzyme, and possibly serves as a signal for the export of the protein.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei154 – 1541SubstrateUniRule annotation
Binding sitei163 – 1631SubstrateUniRule annotation
Active sitei204 – 2041Proton donorUniRule annotation
Metal bindingi241 – 2411MagnesiumUniRule annotation
Metal bindingi290 – 2901MagnesiumUniRule annotation
Binding sitei290 – 2901SubstrateUniRule annotation
Metal bindingi317 – 3171MagnesiumUniRule annotation
Binding sitei317 – 3171SubstrateUniRule annotation
Active sitei342 – 3421Proton acceptorUniRule annotation
Binding sitei342 – 3421Substrate (covalent); in inhibited formUniRule annotation
Binding sitei393 – 3931SubstrateUniRule annotation

GO - Molecular functioni

  1. magnesium ion binding Source: UniProtKB-HAMAP
  2. phosphopyruvate hydratase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. glycolytic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

LyaseUniRule annotation

Keywords - Biological processi

GlycolysisUniRule annotation

Keywords - Ligandi

MagnesiumUniRule annotation, Metal-bindingUniRule annotation

Enzyme and pathway databases

BioCyciBSP600809:GHSF-374-MONOMER.
UniPathwayiUPA00109; UER00187.

Names & Taxonomyi

Protein namesi
Recommended name:
EnolaseUniRule annotation (EC:4.2.1.11UniRule annotation)
Alternative name(s):
2-phospho-D-glycerate hydro-lyaseUniRule annotation
2-phosphoglycerate dehydrataseUniRule annotation
Gene namesi
Name:enoUniRule annotationImported
Ordered Locus Names:BPLAN_372Imported
OrganismiBlattabacterium sp. subsp. Periplaneta americana (strain BPLAN) (Periplaneta americana symbiotic bacterium)Imported
Taxonomic identifieri600809 [NCBI]
Taxonomic lineageiBacteriaBacteroidetesFlavobacteriiaFlavobacterialesBlattabacteriaceaeBlattabacterium
ProteomesiUP000002225 Componenti: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation. Secreted UniRule annotation. Cell surface UniRule annotation
Note: Fractions of enolase are present in both the cytoplasm and on the cell surface. The export of enolase possibly depends on the covalent binding to the substrate; once secreted, it remains attached to the cell surface.UniRule annotation

GO - Cellular componenti

  1. cell surface Source: UniProtKB-SubCell
  2. extracellular region Source: UniProtKB-SubCell
  3. phosphopyruvate hydratase complex Source: InterPro
Complete GO annotation...

Keywords - Cellular componenti

CytoplasmUniRule annotation, SecretedUniRule annotation

Interactioni

Protein-protein interaction databases

STRINGi600809.BPLAN_372.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni369 – 3724Substrate bindingUniRule annotation

Sequence similaritiesi

Belongs to the enolase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0148.
HOGENOMiHOG000072174.
KOiK01689.
OMAiNLPLYRY.
OrthoDBiEOG65J589.

Family and domain databases

Gene3Di3.20.20.120. 1 hit.
3.30.390.10. 1 hit.
HAMAPiMF_00318. Enolase.
InterProiIPR000941. Enolase.
IPR020810. Enolase_C.
IPR029065. Enolase_C-like.
IPR020809. Enolase_CS.
IPR020811. Enolase_N.
IPR029017. Enolase_N_like.
[Graphical view]
PANTHERiPTHR11902. PTHR11902. 1 hit.
PfamiPF00113. Enolase_C. 1 hit.
PF03952. Enolase_N. 1 hit.
[Graphical view]
PIRSFiPIRSF001400. Enolase. 1 hit.
PRINTSiPR00148. ENOLASE.
SUPFAMiSSF51604. SSF51604. 1 hit.
SSF54826. SSF54826. 1 hit.
TIGRFAMsiTIGR01060. eno. 1 hit.
PROSITEiPS00164. ENOLASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

D0J9G5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSKIKTIKAR QILDSRGNPT VEVDVVTEKN VLGRASVPSG ASKGENEAFE
60 70 80 90 100
LRDGGEKFLG NGVMKAVHNV NNIIAPELIG FSVMDQISID KLILDLDGTK
110 120 130 140 150
NKKRLGANAI LGVSLAVVKA ASKELNLPLY KYIGGVHAHI LPIPLMNIVN
160 170 180 190 200
GGRHSDAPIA FQEFMIVPMR ANTFLDAIQM GYKVFYKLKN ILSKKGLSTN
210 220 230 240 250
VGDEGGFSSN FNGIEDVLDN ILEAIHQANY EPYEQIGLAL DCAASEFYQD
260 270 280 290 300
EKYNYSKFEK ENKEKVERSK EEHVNYLSYL TRRYPIISIE DGMDQNDWEG
310 320 330 340 350
WKMLTRELGK EVLLVGDDLF VTKVEKLNEG IKEGIANSIL IKVNQVGTLT
360 370 380 390 400
ETIATINTGK ENGYCNIISH RSGDTEDSFI ADLSVALNIG RIKTGSICRS
410 420
ERTSKYNQLL RIEDMLGKNS HYPEWTG
Length:427
Mass (Da):47,443
Last modified:December 15, 2009 - v1
Checksum:i18E2F2EED017663E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001429 Genomic DNA. Translation: ACX83986.1.
RefSeqiWP_012821513.1. NC_013418.2.
YP_003257129.1. NC_013418.2.

Genome annotation databases

EnsemblBacteriaiACX83986; ACX83986; BPLAN_372.
KEGGibpi:BPLAN_372.
PATRICi31960689. VBIBlaSp127104_0376.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001429 Genomic DNA. Translation: ACX83986.1.
RefSeqiWP_012821513.1. NC_013418.2.
YP_003257129.1. NC_013418.2.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi600809.BPLAN_372.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACX83986; ACX83986; BPLAN_372.
KEGGibpi:BPLAN_372.
PATRICi31960689. VBIBlaSp127104_0376.

Phylogenomic databases

eggNOGiCOG0148.
HOGENOMiHOG000072174.
KOiK01689.
OMAiNLPLYRY.
OrthoDBiEOG65J589.

Enzyme and pathway databases

UniPathwayiUPA00109; UER00187.
BioCyciBSP600809:GHSF-374-MONOMER.

Family and domain databases

Gene3Di3.20.20.120. 1 hit.
3.30.390.10. 1 hit.
HAMAPiMF_00318. Enolase.
InterProiIPR000941. Enolase.
IPR020810. Enolase_C.
IPR029065. Enolase_C-like.
IPR020809. Enolase_CS.
IPR020811. Enolase_N.
IPR029017. Enolase_N_like.
[Graphical view]
PANTHERiPTHR11902. PTHR11902. 1 hit.
PfamiPF00113. Enolase_C. 1 hit.
PF03952. Enolase_N. 1 hit.
[Graphical view]
PIRSFiPIRSF001400. Enolase. 1 hit.
PRINTSiPR00148. ENOLASE.
SUPFAMiSSF51604. SSF51604. 1 hit.
SSF54826. SSF54826. 1 hit.
TIGRFAMsiTIGR01060. eno. 1 hit.
PROSITEiPS00164. ENOLASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Nitrogen recycling and nutritional provisioning by Blattabacterium, the cockroach endosymbiont."
    Sabree Z.L., Kambhampati S., Moran N.A.
    Proc. Natl. Acad. Sci. U.S.A. 106:19521-19526(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: BPLANImported.

Entry informationi

Entry nameiD0J9G5_BLASP
AccessioniPrimary (citable) accession number: D0J9G5
Entry historyi
Integrated into UniProtKB/TrEMBL: December 15, 2009
Last sequence update: December 15, 2009
Last modified: April 1, 2015
This is version 38 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteomeImported

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.