D0GED0 (D0GED0_BRUML) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
Ketol-acid reductoisomerase HAMAP MF_00435
EC=1.1.1.86 HAMAP MF_00435

Alternative name(s):
Acetohydroxy-acid isomeroreductase HAMAP MF_00435
Alpha-keto-beta-hydroxylacil reductoisomerase HAMAP MF_00435

Gene names

Name:	ilvC HAMAP MF_00435
ORF Names:	BASG_00556 EMBL EEZ17711.1

Organism

Brucella melitensis bv. 2 str. 63/9 EMBL EEZ17711.1

Taxonomic identifier

520465 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Brucellaceae › Brucella

Protein attributes

Sequence length	339 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	(2R,3R)-2,3-dihydroxy-3-methylpentanoate + NADP+ = (S)-2-hydroxy-2-ethyl-3-oxobutanoate + NADPH. HAMAP MF_00435 (R)-2,3-dihydroxy-3-methylbutanoate + NADP+ = (S)-2-hydroxy-2-methyl-3-oxobutanoate + NADPH. HAMAP MF_00435
Pathway	Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 2/4. HAMAP MF_00435 Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 2/4. HAMAP MF_00435
Sequence similarities	Belongs to the ketol-acid reductoisomerase family. HAMAP MF_00435

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Branched-chain amino acid biosynthesis HAMAP MF_00435
Ligand	NADP HAMAP MF_00435
Molecular function	Isomerase EMBL EEZ17711.1 Oxidoreductase HAMAP MF_00435
Gene Ontology (GO)
Biological process	isoleucine biosynthetic process Inferred from electronic annotation. Source: HAMAP valine biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	coenzyme binding Inferred from electronic annotation. Source: InterPro isomerase activity Inferred from electronic annotation. Source: UniProtKB-KW ketol-acid reductoisomerase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Sites
	Active site	108	1	By similarity HAMAP MF_00435

Sequences

	Sequence		Length	Mass (Da)
	D0GED0 [UniParc]. Last modified December 15, 2009. Version 1. Checksum: 1A4967D9205EEE92	FASTA	339	37,080
	10 20 30 40 50 60 MRVYYDRDAD VNLIKSKKVV IVGYGSQGRA HALNLKDSGA ANVRVALREG SATVQKAQAD 70 80 90 100 110 120 GFEVMNVADA AKWADLMMMA TPDELQADIY RDHIHNNLRD GAAIAFAHGL NVHCGLIEPK 130 140 150 160 170 180 KTVDVMMIAP KGPGHTVRGE YQKGGGVPCL IAIHQDASGN AHDLALSYAS GVGGGRSGVI 190 200 210 220 230 240 ETTFKEECET DLFGEQAVLC GGVVELIRTG FEVLVEAGYA PEMAYFECLN EMKLIVDLIY 250 260 270 280 290 300 EGGIANMNYS ISNTAEWGEY VTGPRIITAE TKAEMKRVLK DIQTGKFTSD WMQEWKAGAA 310 320 330 RFKGIRRLND AHQIEEVGGK LRAMMPWIEK NKLVDKARN

References

[1]

"The Genome Sequence of Brucella melitensis bv. 2 str. 63/9." The Broad Institute Genome Sequencing Platform Ward D., Young S.K., Kodira C.D., Zeng Q., Koehrsen M., Alvarado L., Berlin A., Borenstein D., Chen Z., Engels R., Freedman E., Gellesch M., Goldberg J., Griggs A., Gujja S., Heiman D., Hepburn T., Howarth C. , Jen D., Larson L., Lewis B., Mehta T., Park D., Pearson M., Roberts A., Saif S., Shea T., Shenoy N., Sisk P., Stolte C., Sykes S., Walk T., White J., Yandava C., Whatmore A.M., Perrett L.L., O'Callaghan D., Nusbaum C., Galagan J., Birren B. Submitted (NOV-2009) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE. Strain: 63/9 EMBL EEZ17711.1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	ACEM01000033 Genomic DNA. Translation: EEZ17711.1.
3D structure databases
ProteinModelPortal	D0GED0.
SMR	D0GED0. Positions 1-328.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
PATRIC	35219873. VBIBruMel34509_2011.
Phylogenomic databases
OMA	MAYAKGI.
Family and domain databases
HAMAP	MF_00435. IlvC. [Tree]
InterPro	IPR008927. 6-PGluconate_DH_C-like. IPR013023. AcH_isomrdctse. IPR000506. AcH_isomrdctse_C. IPR013328. DH_multihelical. IPR013116. IlvN. [Graphical view]
Gene3D	G3DSA:1.10.1040.10. Opine_DH. 1 hit.
PANTHER	PTHR21371. AcH_isomrdctse. 1 hit.
Pfam	PF01450. IlvC. 1 hit. PF07991. IlvN. 1 hit. [Graphical view]
SUPFAM	SSF48179. 6DGDH_C_like. 1 hit.
TIGRFAMs	TIGR00465. IlvC. 1 hit.
ProtoNet	Search...

Entry information

Entry name

D0GED0_BRUML

Accession

Primary (citable) accession number: D0GED0

Entry history

Integrated into UniProtKB/TrEMBL:	December 15, 2009
Last sequence update:	December 15, 2009
Last modified:	December 14, 2011
This is version 14 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)