Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

D0FPH9 (D0FPH9_ERWPE) Unreviewed, UniProtKB/TrEMBL

Last modified June 11, 2014. Version 29. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry infoCustomize order

Names and origin

Protein namesRecommended name:
D-alanine--D-alanine ligase HAMAP-Rule MF_00047

EC=6.3.2.4 HAMAP-Rule MF_00047
Alternative name(s):
D-Ala-D-Ala ligase HAMAP-Rule MF_00047
D-alanylalanine synthetase HAMAP-Rule MF_00047
Gene names
Name:ddl HAMAP-Rule MF_00047 EMBL CAX54524.1
Ordered Locus Names:EpC_07450 EMBL CAX54524.1
OrganismErwinia pyrifoliae (strain Ep1/96) [Complete proteome] [HAMAP]
Taxonomic identifier634499 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeErwinia

Protein attributes

Sequence length306 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Cell wall formation By similarity. HAMAP-Rule MF_00047 SAAS SAAS011095

Catalytic activity

ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine. HAMAP-Rule MF_00047 SAAS SAAS011095

Cofactor

Binds 2 magnesium or manganese ions per subunit By similarity. HAMAP-Rule MF_00047 SAAS SAAS011095

Pathway

Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP-Rule MF_00047 SAAS SAAS011095

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00047 SAAS SAAS011095.

Sequence similarities

Belongs to the D-alanine--D-alanine ligase family. HAMAP-Rule MF_00047

Contains 1 ATP-grasp domain. HAMAP-Rule MF_00047

Contains ATP-grasp domain. SAAS SAAS011095

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Regions

Domain101 – 303203ATP-grasp By similarity HAMAP-Rule MF_00047
Nucleotide binding134 – 18956ATP By similarity HAMAP-Rule MF_00047

Sites

Metal binding2571Magnesium or manganese 1 By similarity HAMAP-Rule MF_00047
Metal binding2701Magnesium or manganese 1 By similarity HAMAP-Rule MF_00047
Metal binding2701Magnesium or manganese 2 By similarity HAMAP-Rule MF_00047
Metal binding2721Magnesium or manganese 2 By similarity HAMAP-Rule MF_00047

Sequences

Sequence LengthMass (Da)Tools
D0FPH9 [UniParc].

Last modified November 24, 2009. Version 1.
Checksum: AF80CB16DE6FE3AA

FASTA30632,873
        10         20         30         40         50         60 
MAEKVAVLLG GTSAERDVSL LSGAAVLKGL QEAGIDAHAV DIRDFPVTRL NEEGFDKAFI 

        70         80         90        100        110        120 
ALHGRGGEDG TLQGVLELLD IPYTGSGVMA SAITMDKLRS KYLWQGCGLP VSPFIALNRV 

       130        140        150        160        170        180 
QMDAGLDAQM MTSINALGLP LFVKPSREGS SVGISRVNQT SELQAALVEA FRHDDEVLVE 

       190        200        210        220        230        240 
AFLSGPEYTV GVLGSDILPS IRIQTSSEFY DYDAKYISDE TQYFCPSGLS ADQETELREL 

       250        260        270        280        290        300 
TIAAWRALGC SGWGRVDVMM GADGHFYLLE VNTSPGMTSH SLVPMAAKQA GMTFSQLVVR 


ILELAD 

« Hide

References

[1]"Genome comparison of the epiphytic bacteria Erwinia billingiae and E. tasmaniensis with the pear pathogen E. pyrifoliae."
Kube M., Migdoll A.M., Gehring I., Heitmann K., Mayer Y., Kuhl H., Knaust F., Geider K., Reinhardt R.
BMC Genomics 11:393-393(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Ep1/96 EMBL CAX54524.1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
FP236842 Genomic DNA. Translation: CAX54524.1.
RefSeqYP_002647775.1. NC_012214.1.

3D structure databases

ProteinModelPortalD0FPH9.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING634499.EpC_07450.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAX54524; CAX54524; EpC_07450.
GeneID8541075.
KEGGepy:EpC_07450.
PATRIC20419692. VBIErwPyr67807_0757.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMHOG000011592.
KOK01921.
OMAEDPARES.
OrthoDBEOG6ND0KB.

Enzyme and pathway databases

BioCycEPYR634499:GJIP-786-MONOMER.
UniPathwayUPA00219.

Family and domain databases

Gene3D3.30.1490.20. 1 hit.
3.30.470.20. 2 hits.
3.40.50.20. 1 hit.
HAMAPMF_00047. Dala_Dala_lig.
InterProIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR000291. D-Ala_lig_Van_CS.
IPR005905. D_ala_D_ala.
IPR011095. Dala_Dala_lig_C.
IPR011127. Dala_Dala_lig_N.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PANTHERPTHR23132. PTHR23132. 1 hit.
PfamPF07478. Dala_Dala_lig_C. 1 hit.
PF01820. Dala_Dala_lig_N. 2 hits.
[Graphical view]
SUPFAMSSF52440. SSF52440. 1 hit.
TIGRFAMsTIGR01205. D_ala_D_alaTIGR. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
PS00843. DALA_DALA_LIGASE_1. 1 hit.
PS00844. DALA_DALA_LIGASE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameD0FPH9_ERWPE
AccessionPrimary (citable) accession number: D0FPH9
Entry history
Integrated into UniProtKB/TrEMBL: November 24, 2009
Last sequence update: November 24, 2009
Last modified: June 11, 2014
This is version 29 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)